LOCUS       pCMV4-HA-p31_com        5785 bp DNA     circular SYN 22-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
COMMENT     Sequence Label: pCMV4-HA-p31 comet
FEATURES             Location/Qualifiers
     source          1..5785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             690..779
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    1636..2258
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2287..2616
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2467..2602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2655..2751
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(2828..2846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2860..2876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2884..2900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2908..2914,2915..2932,2933..2938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2953..2974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3262..3850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4021..4812,4813..4881))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4882..4986)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5013..5468
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5609..5625
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cmvhacmtga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag aattgttttt atttttaatt ttctttcaaa tacttccatc gaattcagat
      661 ctggtaccac gcgtaccatg gatatctttt acccatacga tgttcctgac tatgcgggct
      721 atccctatga cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg
      781 ctcagatcga taagcttatg gcggcgccgg aggcggaggt tctgtcctca gccgcagtcc
      841 ctgatttgga gtggtatgag aagtccgaag aaactcacgc ctcccagata gaactacttg
      901 agacaagctc tacgcaggaa cctctcaacg cttcggaggc cttttgccca agagactgca
      961 tggtaccagt ggtgtttcct gggcctgtga gccaggaagg ctgctgtcag tttacttgtg
     1021 aacttctaaa gcatatcatg tatcaacgcc agcagctccc tctgccctat gaacagctta
     1081 agcactttta ccgaaaacct tctccccagg cagaggagat gctgaagaag aaacctcggg
     1141 ccaccactga ggtgagcagc aggaaatgcc aacaagccct ggcagaactg gagagtgtcc
     1201 tcagccacct ggaggacttc tttgcacgga cactagtacc gcgagtgctg attctccttg
     1261 ggggcaatgc cctaagcccc aaggagttct atgaactcga cttgtctctg ctggccccct
     1321 acagcgtgga ccagagcctg agcacagcag cttgtttgcg ccgtctcttc cgagccatat
     1381 tcatggctga tgcctttagc gagcttcagg ctcctccact catgggcacc gtcgtcatgg
     1441 cacagggaca ccgcaactgt ggagaagatt ggtttcgacc caagctcaac tatcgagtgc
     1501 ccagccgggg ccataaactg actgtgaccc tgtcatgtgg cagaccttcc atccgaacca
     1561 cggcttggga agactacatt tggttccagg caccagtgac atttaaaggc ttccgcgagt
     1621 gatctagagg atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     1681 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     1741 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     1801 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     1861 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     1921 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     1981 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     2041 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     2101 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     2161 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     2221 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg
     2281 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2341 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2401 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2461 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2521 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     2581 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac
     2641 cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc ggatccggtg
     2701 gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg
     2761 gaagtgttac ttctgctcta aaagctgctg caggtcgact ctagggccgc gtaccgagct
     2821 cgaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     2881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3061 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4981 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     5041 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5101 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5161 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5221 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5281 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5341 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5401 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5461 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5521 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5581 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgatctat
     5641 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     5701 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     5761 tcatgtccaa tatgaccgcc atgtt
//
