LOCUS       Exported                5026 bp ds-DNA     circular SYN 15-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 2, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..30
                     /label=TP18
                     /label=TP18, with 5'-aatataGCT
     misc_feature    10..1161
                     /label=DONOR LEFT ARM
     CDS             1078..1173
                     /codon_start=1
                     /label=Exon
                     /translation="VIYTIFSPEFRQAFKRILFGGHRPVHYRSGKL"
     primer_bind     complement(1153..1181)
                     /label=TP20
     misc_feature    1162..1695
                     /label=INSERT-534 bp
     primer_bind     1162..1189
                     /label=TP21
     CDS             1174..1218
                     /label=C-linker
     primer_bind     complement(1176..1194)
                     /label=P32
     CDS             1219..1266
                     /label=GFP11
     CDS             1267..1299
                     /label=linker2
     CDS             1300..1347
                     /label=GFP11
     CDS             1363..1410
                     /label=GFP11-COPY2
     CDS             1426..1473
                     /label=GFP11-COPY3
     CDS             1489..1536
                     /label=GFP11-COPY4
     CDS             1552..1599
                     /label=GFP11-COPY5
     CDS             1615..1662
                     /label=GFP11-COPY6
     CDS             1666..1692
                     /label=HA
     primer_bind     complement(1668..1705)
                     /label=TP22
     primer_bind     1681..1717
                     /label=TP23
     misc_feature    1696..2798
                     /label=DONOR RIGHT ARM
     misc_feature    1699..1701
                     /label=PAM GGG to GGa
     misc_feature    complement(2777..2806)
                     /label=TP19, with 5'-aataGCGG
     primer_bind     complement(2865..2881)
                     /label=M13 rev
     primer_bind     complement(2878..2899)
                     /label=M13R
     rep_origin      complement(3053..3641)
                     /direction=LEFT
                     /label=ori
     protein_bind    complement(3758..3782)
                     /label=attB1
     CDS             complement(3882..4742)
                     /label=AmpR
     promoter        complement(4743..4847)
                     /label=AmpR promoter
     primer_bind     4961..4977
                     /label=M13 fwd
ORIGIN
        1 aattatgcta gctagaggac caggacgagc aggttggccc gcagcccaca accgcaacat
       61 cagcaatgac agcggcaggc accaacgagt cggaggacca atgcaaggcc aacggggtgg
      121 aggtcctgga ggatccgcag ttgcaacagc agctcgaaca agtgcagcag ctgcagaaat
      181 cggttaaatc cggcggtgga ggtggggcca gtacgtcaaa tgccaccaca attacgtcga
      241 tatcagcgct atcgccacaa acgccaacat cgcagggagt tggaatcgcc gccgccgccg
      301 ctggaccgat gacggccaaa accagcacgc tgacgagctg caaccagtcg catccgctat
      361 gcggcaccgc caacgaatcc ccatcgacac cagagccacg tagccgacaa ccgacgaccc
      421 cgcagcagca gccgcatcag caggcgcacc agcagcagca gcagcagcag caactctcca
      481 gcatcgccaa tccgatgcag aaggtgaaca agcgaaagga gaccctggag gccaaaaggg
      541 aacggaaggc ggccaagacg ctggccatca ttaccggcgc cttcgtcgtc tgctggctgc
      601 ccttcttcgt catggcactg accatgccgc tctgtgccgc ctgccagatc agcgattccg
      661 ttgcctcgct cttcctttgg ctgggctact tcaactccac cctcaatccg gtaaatggaa
      721 taataaataa atggctaagt aactgcagga atagtgcaaa actttgcata tatagagaaa
      781 ttttaagact tttaaacttc gcaacttgtc tgtgttcttt gaaaagagtt tttaaactat
      841 tagttacttt gaattttaat aatgaacttt taaaaatttg agagcatagt ttataataat
      901 atactcaaac tacagataga cttcttaatc ttaatctatt tcaaataatc agacaattta
      961 tgaaaaaaat tagagtttct taactcgttt tagttactta aattgttgtc aaatcaaatg
     1021 gttatgagtg cagttttcat tcccgtgcat tgttgattaa gtaattcccc cttgcaggtt
     1081 atttacacca tcttcagtcc ggagttccga caggccttca agcgcatcct cttcggcggc
     1141 caccgaccag tccactaccg cagcgggaag ctcggcgatg gaggaagcgg aggcgacgga
     1201 ggcagcggag gaggcagccg ggaccacatg gtgctgcacg agtacgtgaa cgccgccggc
     1261 atcactagcg gaggcgacgg aggcagcgga ggaggcagcc gggaccacat ggtgctgcac
     1321 gagtacgtga acgccgccgg catcactagc ggtagtggcg gccgtgacca catggtcctt
     1381 catgagtatg taaatgctgc tgggattaca ggtggctctg gaggtagaga tcatatggtt
     1441 ctccacgaat acgttaacgc cgcaggcatc actggcggta gtggaggacg cgaccatatg
     1501 gtactacatg aatatgtcaa tgcagccgga ataaccggag ggtccggagg ccgggatcac
     1561 atggtgctgc atgagtatgt gaacgcggcg ggtataactg gtgggtcggg cggacgagac
     1621 catatggtgc ttcacgaata cgtaaacgca gctggcatta ctagttaccc atacgatgtt
     1681 ccagattacg cttaaagcgg aaagctctag atagtgcatg cgaagaggcg aaggtgagtg
     1741 ctccttgggg aagccaatac tcggcaatga aggtgcactg acagaaaaat atatgaccca
     1801 cttctcacat aaccataaat actaacaaga atttctctgt taggttatga aattaatcat
     1861 aaagctgttc aaaagtacgc agtaaaattt tattgcttaa aacgttgcat acttttaggt
     1921 tactttaact tatttacatg gctttatcta gcagactttc gttattctag caattgttta
     1981 cttatattgt ttaaaaaata tgaaaattgt tttccatgca ccttgtcctt gtgtgccgca
     2041 gttattgtta ttgagttaga caatttagcc gacaaccatt ggaaacattt tgaatcgatt
     2101 caacgcacac gcaattgcat tctttttctg ctattgtccc tagctgacaa gtttcaattt
     2161 tcaatgctcg aagccagacc cgaaattgat attgcctgag ttgagtggaa agtgcagcag
     2221 tttgcaaagg aaccgaaacc attgtttagc tttaaaaaaa aaaaaaagaa ccaaagggga
     2281 gaactttctg aacctagagc caaagtttta tcagaccttt atcggacctg tcatctgcac
     2341 ccagcaaacc atccaagcaa ccgaccagcc atgttggtca atgcattaga attatgccac
     2401 acagctcggt ggaaacattg ccgcttgaaa agcaagctaa accgaaatct aaatcgggaa
     2461 atgggttttt atctacaaaa agtgagcggg aatggcgcgc acaagttttg aatagttacc
     2521 atttgagttt gctgaaggaa gagccatctg gatgtggcag cagttaaatc tttgccattc
     2581 attcgttttc atttgcaaaa ggcaacatga aagttaaatt gagtaacatt aatttgaaga
     2641 atttcctgct tagttgggcg ccccgccatt tcgatgaact tttctgtgaa tgcacaaagt
     2701 ttttgagcac cccaatgccc cccctcaatt cacacttcac taatgaatcc ttgcaattgt
     2761 aacatccttg cagacgcttc tgctaaaccg accagcggcc gcaattatag gctaggtgga
     2821 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
     2881 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
     2941 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
     3001 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3061 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3121 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3181 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3241 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3301 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3361 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3421 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3481 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3541 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3601 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3661 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     3721 tagggggctt caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt
     3781 gttgataatg gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4021 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4861 aaagtgccag atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     4921 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4981 gcataatccg cacgcatctg gaaggaagtg ccattccgcc tgacct
//
