LOCUS       C47_pACUH_5-HT2B       12662 bp DNA     circular SYN 21-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
     CDS             complement(994..1785)
                     /label=AmpR
     CDS             complement(1786..1854)
                     /label=AmpR
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     protein_bind    2434..2503
                     /label=attB
     misc_feature    3046..3076
                     /label=P element 3' end
     misc_feature    3077..3278
                     /label=P element 3' end
     protein_bind    3359..3453
                     /label=5X UAS
     protein_bind    3477..3571
                     /label=5X UAS
     promoter        3590..3828
                     /label=hsp70 promoter
     primer_bind     3685..3703
                     /label=P6
     primer_bind     3775..3796
                     /label=pCasper-hs
     misc_feature    3863..3872
                     /label=Kozak
     CDS             3869..6580
                     /codon_start=1
                     /label=5-HT2B
                     /translation="MEEDVYASLGAYNDSGGDDWSSSEHLVLWEEDETQRTTANATSRH
                     NQLHVARWNATGNATISATFEDVPFDANNYWALLALVLVLGTAAGNILVCLAIAWERRL
                     QNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMH
                     LCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHASVLVN
                     GTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWAS
                     GWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKE
                     PTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAA
                     THQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGE
                     QSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLP
                     KNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQN
                     HQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMC
                     SSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGT
                     PCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSA
                     TLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIR
                     LEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAECEERISHWVFDVVTWLGYASSMVN
                     PIFYTIFNKVFRQAFKKVLLCRYSSTSAWRPSR"
     primer_bind     6453..6471
                     /label=P103
     primer_bind     6533..6556
                     /label=P62
     primer_bind     complement(6533..6556)
                     /label=P61
     misc_feature    complement(6544..6546)
                     /label=PAM CGG to CGA
     misc_feature    complement(6547..6566)
                     /label=gRNA2
     CDS             6584..6628
                     /codon_start=1
                     /label=C-linker
                     /translation="GDGGSGGDGGSGGGS"
     primer_bind     complement(6586..6604)
                     /label=P32
     CDS             6629..6676
                     /codon_start=1
                     /label=GFP11
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6677..6709
                     /codon_start=1
                     /label=linker2
                     /translation="SGGDGGSGGGS"
     CDS             6710..6757
                     /codon_start=1
                     /label=GFP11
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6773..6820
                     /codon_start=1
                     /label=GFP11-COPY3
                     /label=GFP11-COPY2
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6836..6883
                     /codon_start=1
                     /label=GFP11-COPY4
                     /label=GFP11-COPY3
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6899..6946
                     /codon_start=1
                     /label=GFP11-COPY5
                     /label=GFP11-COPY4
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             6962..7009
                     /codon_start=1
                     /label=GFP11-COPY6
                     /label=GFP11-COPY5
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7025..7072
                     /codon_start=1
                     /label=GFP11-COPY7
                     /label=GFP11-COPY6
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(7064..7088)
                     /label=P75
     CDS             7076..7102
                     /codon_start=1
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7078..7105)
                     /label=TP43
     primer_bind     7106..7129
                     /label=TP44
     misc_feature    complement(7115..7117)
                     /label=PAM GGG to GGT
     misc_feature    complement(7118..7137)
                     /label=gRNA1
     primer_bind     complement(join(7175..7195,7196..7200))
                     /label=TP52, with 5'-aata
                     /label=TP52
     misc_feature    complement(7257..7276)
                     /label=P10
     intron          7291..7356
                     /label=small t intron
     CDS             7486..7506
                     /label=SV40 NLS
     polyA_signal    7778..7912
                     /label=SV40 poly(A) signal
     CDS             8417..8488
                     /label=mini-white
     CDS             8417..8488
                     /label=mini-white
     CDS             8489..8882
                     /label=mini-white
     CDS             8489..8882
                     /label=mini-white
     CDS             8883..9156
                     /label=mini-white
     CDS             8883..9156
                     /label=mini-white
     CDS             9157..9230
                     /label=mini-white
     CDS             9157..9230
                     /label=mini-white
     CDS             9231..9885
                     /label=mini-white
     CDS             9231..9885
                     /label=mini-white
     CDS             9886..9946
                     /label=mini-white
     CDS             9886..9946
                     /label=mini-white
     CDS             9947..10262
                     /label=mini-white
     CDS             9947..10262
                     /label=mini-white
     CDS             10263..10482
                     /label=mini-white
     CDS             10263..10482
                     /label=mini-white
     CDS             10483..10614
                     /label=mini-white
     CDS             10483..10614
                     /label=mini-white
     CDS             10615..10684
                     /label=mini-white
     CDS             10615..10684
                     /label=mini-white
     CDS             10685..11299
                     /label=mini-white
     CDS             10685..11299
                     /label=mini-white
     misc_feature    12077..12631
                     /label=P element 5' end
     misc_feature    12077..12631
                     /label=P element 5' end
     misc_feature    12632..12662
                     /label=P element 5' end
     misc_feature    12632..12662
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3001 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3061 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3121 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3181 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3241 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc atccaacgcg
     3301 ttgggagctc tccggatcca agctggccgc ggctcgatcc gcttgcatgc ctgcaggtcg
     3361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3421 tactgtcctc cgagcggagt actgtcctcc gagcggaagc ttgcatgcct gcaggtcgga
     3481 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3541 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     3601 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     3661 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     3721 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     3781 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     3841 ggaattccgg aatttcctcg aggccaccat ggaagaggat gtgtatgcct cgctaggtgc
     3901 ctacaacgac agcggtggcg acgattggag cagctcggag cacctggtcc tgtgggagga
     3961 ggatgagacg cagcgaacga ctgctaatgc caccagtcgg cataatcaac tgcatgtggc
     4021 caggtggaat gccaccggca atgcgaccat cagcgcgacc ttcgaggacg tacccttcga
     4081 tgcgaacaac tactgggccc tattggccct cgtcctcgtc ctgggaacag cggccgggaa
     4141 catccttgtc tgcctggcca tcgcctggga gcgccggctg cagaatgtga ccaactactt
     4201 ccttatgtcg ctggccatca ccgatctcat ggtcgccgtg ctggtcatgc cgctgggcat
     4261 ccttacgctg gtgaaaggct actttccttt gggctcggag cactgcctca cctggatctg
     4321 cctggatgta ctcttctgca cggccagcat catgcacctg tgcaccatct ccgtggacag
     4381 atatttatcg ctacgatacc caatgcgatt tggccggaat aaaacacgtc ggcgggtcac
     4441 tctcaaaatt gttttcgttt ggcttctgag catcgccatg agcttgccct tgagcttgat
     4501 gtactccaag aatcacgcct cggtgctggt gaatggaact tgccagatac cggatccggt
     4561 gtacaagctg gtcggctcca ttgtatgctt ctacattcca ctgggcgtga tgctgctgac
     4621 atattgcctg accgtccgac ttttggcccg gcagcgccag aatctgggtg gtggacagca
     4681 gacggcggcg gccactcccg gatgggccag tggatggctc ggccaggcac cggccttgga
     4741 acgacgatgc acctggcggc gccttttgaa accgggtcca ggtaacgcct cctcggtgct
     4801 gcacgcacac tcggccaatt caacggatac ggatctcagc actttggaca accacgagct
     4861 ctggctgccc gattccagca taaaggagcc aacgcccacg accatgacgg ccttgcatca
     4921 gttcggtgcc gagatgctga aactatcccg aggcctggag tcggtggcct cgtcatccac
     4981 caccggctca cccaccaaat ccgaattttc cctatcgaac cacctgcagc tgcagtaccc
     5041 cagcagccca cagcgatatg ccgccaccca ccagcagtcc caccaccaca accaccagca
     5101 gcatccggcc ggagtttacc accagcaggc atcgcccaaa ggtcgccacg gaaccacagt
     5161 tctgggtctg tccaccacca cgctgggcat cgagcgggag agcaccagga attccctggc
     5221 cagcagtcgc atgggcggcg aacagagcga tgggactctt tcacagcttt cccaacgatt
     5281 gcgagcgtat aaaaagcgac gaagagcttc gtcagctgtt cctggacgtg agaaacgtgc
     5341 cgggcatgag gacgcagacg aggatgtgga aacgcccacg tcaacgctgc gccgccacaa
     5401 gcgccataac agtctgccca agaacgcact ctatccgcga cacaccactg tacaggagag
     5461 cctcgatgac gacgacgacg aggaggagga gagcgcgcaa cagaaggatc agcagacgaa
     5521 gtcgaagatc tgccactcgg acactgagct agatcctccg cagaaggcga agcagtgcca
     5581 catagccggg cagaatcatc agtcggacta cctgcagctg ccctcggttt gcacttgtcc
     5641 gtattttggg gacagaccgc tccagaattg tgtgaaatcg gcggaggtga agattatttc
     5701 gtccgccttc cgagtgacca ccaccacgac ggcggtgagc agttcgccca gcgagatgga
     5761 gctactcatg tgcagtagtg gcaataaaaa gtcattgacc tccagtgtga gtgccggcat
     5821 aacaggtgga ccggcaggag cggctccagc cacagttggc tccaccctca gtccacactc
     5881 tgctcacaat cagggcagct cgctgacagt tcagagcgat ggtagtgggt acttggctgc
     5941 tccgggaact ccttgtcctg gccgacgaaa gttgagcatc tcaaagaccg cctcggtggt
     6001 cacgtgggat tcaagtcgtc atcgtcggcg gggcagcagt tttggcggag tgcgaacatc
     6061 cctgctgttg acgcccacca aaacagcgac cacctccaac tcatccacac cgttgagaag
     6121 atcggccacc ttgaggagtc accagaatat gaactaccag ggagcgggcg aatgtggtgg
     6181 caagaccagg acgacaacat cctcaccctg catgttgcag cgtcagcaga cggtgcgatc
     6241 tcatcactcg cggaactcca gcgtgatctc cagaaactcc tcgcgccacg gcaggatcat
     6301 caggctggag cagaaggcca ccaaggtgct gggagtggtg ttctttacgt tcgtaatcct
     6361 ctggtcgcca ttcttcgtcc tcaatctcct gcccacggtg tgcgcggagt gcgaggaacg
     6421 aattagccac tgggtcttcg acgtggtcac ctggctgggc tacgctagct ccatggtgaa
     6481 ccccatcttc tacaccatct tcaacaaggt gttccggcag gcgttcaaga aggtcctgct
     6541 gtgtcgctat tcgagcacga gtgcctggcg accgagcaga ctcggcgatg gaggaagcgg
     6601 aggcgacgga ggcagcggag gaggcagccg ggaccacatg gtgctgcacg agtacgtgaa
     6661 cgccgccggc atcactagcg gaggcgacgg aggcagcgga ggaggcagcc gggaccacat
     6721 ggtgctgcac gagtacgtga acgccgccgg catcactagc ggtagtggcg gccgtgacca
     6781 catggtcctt catgagtatg taaatgctgc tgggattaca ggtggctctg gaggtagaga
     6841 tcatatggtt ctccacgaat acgttaacgc cgcaggcatc actggcggta gtggaggacg
     6901 cgaccatatg gtactacatg aatatgtcaa tgcagccgga ataaccggag ggtccggagg
     6961 ccgggatcac atggtgctgc atgagtatgt gaacgcggcg ggtataactg gtgggtcggg
     7021 cggacgagac catatggtgc ttcacgaata cgtaaacgca gctggcatta ctagttaccc
     7081 atacgatgtt ccagattacg cttaataaca gacgaccgtt aaaccgggca aactgaagac
     7141 gaatacccac aagtgcgcca gtgtggattt tcaggattcc actgctccgg tgcggccgct
     7201 agactagtct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     7261 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     7321 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     7381 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     7441 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     7501 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     7561 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     7621 ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat
     7681 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7741 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccactagaa
     7921 ggcctaattc tagtatgtat gtaagttaat aaaacccttt tttggagaat gtagatttaa
     7981 aaaaacatat ttttttttta ttttttactg cactggacat cattgaactt atctgatcag
     8041 ttttaaattt acttcgatcc aagggtattt gaagtaccag gttctttcga ttacctctca
     8101 ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat
     8161 atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacc
     8221 tagcgtcaat gtccgccttc agttgcactt tgtcagcggt ttcgtgacga agctccaagc
     8281 ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt
     8341 tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt
     8401 gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa
     8461 acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc
     8521 acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac
     8581 ccacaaaaat cccaaaccgc aatcgcacaa acaaatagtg acacgaaaca gattattctg
     8641 gtagctgtgc tcgctatata agacaatttt taagatcata tcatgatcaa gacatctaaa
     8701 ggcattcatt ttcgactaca ttctttttta caaaaaatat aacaaccaga tattttaagc
     8761 tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcgtt
     8821 ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc
     8881 agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa
     8941 actacggcac gctcctgcca cccagtccgc cggaggactc cggttcaggg agcggccaac
     9001 tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc
     9061 cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac
     9121 acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct
     9181 gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt
     9241 ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct
     9301 gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg
     9361 actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca
     9421 gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt tccaggccat
     9481 ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat
     9541 ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa
     9601 aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc
     9661 gccgcttctg atctgcgatg agcccacctc cggactggac tcatttaccg cccacagcgt
     9721 cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca
     9781 gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt
     9841 agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt
     9901 ttattaaggg tatctagcat tacattacat ctcaactcct atccagcgtg ggtgcccagt
     9961 gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac
    10021 gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgct attagcaaag
    10081 tagcccggga tatggagcag ttgttggcca ccaaaaattt ggagaagcca ctggagcagc
    10141 cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc
    10201 gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa
    10261 cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc
    10321 gctagatttt agttagaatt gcctgattcc acacccttct tagttttttt caatgagatg
    10381 tatagtttat agttttgcag aaaataaata aatttcattt aactcgcgaa catgttgaag
    10441 atatgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
    10501 ggcctcatct ttttgggcca acaactcacg caagtgggcg tgatgaatat caacggagcc
    10561 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
    10621 cttgtttaga atacatttgc atattaataa tttactaact ttctaatgaa tcgattcgat
    10681 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
    10741 ctgtgacaca tactttctgg gcaaaacgat tgccgaatta ccgctttttc tcacagtgcc
    10801 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
    10861 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
    10921 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
    10981 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
    11041 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
    11101 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
    11161 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
    11221 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
    11281 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc
    11341 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
    11401 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
    11461 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
    11521 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
    11581 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
    11641 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
    11701 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
    11761 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
    11821 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
    11881 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
    11941 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
    12001 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
    12061 aactagaatt aattcggctg ctgctctaaa cgacgcattt cgtactccaa agtacgaatt
    12121 ttttccctca agctcttatt ttcattaaac aatgaacagg acctaacgca cagtcacgtt
    12181 attgtttaca taaatgattt tttttactat tcaaacttac tctgtttgtg tactcccact
    12241 ggtatagcct tcttttatct tttctggttc aggctctatc actttactag gtacggcatc
    12301 tgcgttgagt cgcctccttt taaatgtctg accttttgca ggtgcagcct tccactgcga
    12361 atcattaaag tgggtatcac aaatttggga gttttcacca aggctgcacc caaggctctg
    12421 ctcccacaat tttctcttaa tagcacactt cggcacgtga attaatttta ctccagtcac
    12481 agctttgcag caaaatttgc aatatttcat ttttttttat tccacgtaag ggttaatgtt
    12541 ttcaaaaaaa aattcgtccg cacacaacct ttcctctcaa caagcaaacg tgcactgaat
    12601 ttaagtgtat acttcggtaa gcttcggcta tcgacgggac caccttatgt tatttcatca
    12661 tg
//
