LOCUS       GR721_-_pCAN1int.ISceI 10020 bp ds-DNA     circular     30-NOV-2023
DEFINITION  .
KEYWORDS    "creator:Ria Deshpande" "marker:AmpR"
FEATURES             Location/Qualifiers
     primer          180..199
                     /label=2792
                     /note="sequence: CTTAGGGTTAGGCGTTTTGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    242..247
                     /label="EcoRIsite"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    248..927
                     /label="TDH3 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          260..277
                     /label=1622
                     /note="sequence: ATCATTATCAATACTGCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          846..870
                     /label=186
                     /note="sequence: ATTCTACTTTTATAGTTAGTCTTTT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             928..1635
                     /label="I-SceI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             928..1635
                     /label="Translation 928-1635"
                     /translation="MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQFEWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNNKKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLRNKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK*"
     primer          1636..1661
                     /label=6376
                     /note="sequence: ataaagcaatcttgatgaggataatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      1636..1859
                     /label="TDH1 terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1816..1832)
                     /label=1623
                     /note="sequence: GACGTTCTCGCCATAAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1860..1865
                     /label="EcoRIsite"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1866..1897
                     /label="8903 - GR-CAN1.US-fwd"
                     /note="sequence: TATATCTTTAACAGATTCCAAACCCTAAAGTG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1866..2365
                     /label="CAN1 upsteam flank"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1879..1899)
                     /label="C900 - GR-CAN1.USflank-rvs"
                     /note="sequence: GACACTTTAGGGTTTGGAATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1884..1915
                     /label="E063 - GR-CAN1.USflank-fwd"
                     /note="sequence: CAAACCCTAAAGTGTCCGAATTTTCAATAGGG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2334..2365)
                     /label="8904 - GR-CAN1.US-rvs"
                     /note="sequence: TGCTATGCCTTTTTTTTTTTTTGTTTTTACAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2366..3065
                     /label="SLD3p"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3066..3093
                     /label=846
                     /note="sequence: ATGGACTACAAAGACAAAGCATTAAACG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3066..6029
                     /label="Translation 3066-6029"
                     /translation="MDYKDKALNDLRNVYADFDSLPLDFRQILIKDRATLLQKEDVEKKILERQEDAKKYAEYLKQSEIPERISLPNIKRHKGVSISFEETSEDMVLEPRPFIFDGLNIRCFRRETIFSLKNKILNMVKESSSFKNVSRQSVSFMYFKIFNKGKVIASTKSVNIYEDKIDERLEDLCNNFDDVLKKIIDVTYGYESLFVSETYSYVIFYAKSIYFPQPRCVNNWGNNIPNILTFDSFKLFTANKNNVSCIKQCSRFLWQKDFNTLEEMIEYKNGNICIVTPQLHINDVRDIKSFNDIRLYSESPIKTFSVIDNTITYLFYFKEHLGVIFNITKSRHDRRVTKFSPLSKFSDVKNITVCFDIESYFDPEKESNQVNIPFICCASIIYNKVIGNIVDFEGRDCVAQMIEYVVDICGELNISSVELIAHNGGGYDFHYILSSMYNPAAIKNILIRNNSFISFNFAHDGVKFSVKDSYSFLSCSLANASKAFLNEGTFKKTDFPHHDLKTADDLYKVYKEWSSVNTEINHVVEKEKLLITSEHIVNFTKNDKSKTLIEWSKDYCRNDVLVLSKVWLEFKNAVEDIFNCELVDQTMTLAGLSYKLFQANMPFDVELRHPNKEDYFNMREALIGGRCISVNGIYRDVLCLDVKSLYPASMAFYDQPYGSFKRVSSRPKDELGIYYVRVTTNRNNKSNFFPIRSHNKITYNNFEESTYIAWYTNVDIDIGLSEGHNIEYIPFDSYGNIGYSWSKKGKIFEKYIRDVLYKLKIKYEKQNNKVKRNVIKKIMNSLWGKFAQKWVNFEYFIKSEDDIDFESEEAYKIWDTDFMLIKKIKESIYSSKPIQNGVFTLSWARYHMKSIWDAGAKEGAECIYSDTDSIFVHKEHFKKNAKFMLNGLKVPIIGSEVGQSELECEFDKLLCAGKKQYMGFYTYFQDGKPCIKEKKRFKGIPSNYIIPELYAHLLSGADKEAKIQYLKFRREWGSVKGYIENKTVKAT*"
     misc_feature    3066..6029
                     /label="BadBoy2.TPDNAP (trxsgk23)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          3688..3713
                     /label=9383
                     /note="sequence: caatctactttccacaacctagatgc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4485..4487
                     /label="L474S"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4527..4529
                     /label="E488G"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4968..4970
                     /label="K635R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5103..5105
                     /label="P680T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5322..5324
                     /label="K753R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5394..5396
                     /label="I777K"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5547..5549
                     /label="T828I"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    5763..5765
                     /label="L900S"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6003..6030
                     /label="B030"
                     /note="sequence: gaaaataagactgtcaaagccacctaac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      6036..6201
                     /label="ADH1 terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6162..6221
                     /label="8911 - GR-TEFp-fwd_GO:ADH1t"
                     /note="sequence: taggtcaggttgctttctcaggtatagcatgaggtcgctcgacatggaggcccagaatac"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(6178..6201)
                     /label=3651
                     /note="sequence: gagcgacctcatgctatacctgag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        6202..6545
                     /label="Ashbya gossypii TEF promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     gene            6202..7321
                     /label="yeast selectable marker conferring nourseothricin resistance"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             6546..7118
                     /label="Streptomyces noursei nat1 CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     terminator      7124..7321
                     /label="Ashbya gossypii TEF terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(7300..7359)
                     /label="8912 - GR-TEFt-rvs_GO:CAN1.DS"
                     /note="sequence: GGCGTGGAAATGTGATCAAAGGTAATAAAACGTCATATcagtatagcgaccagcattcac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          7322..7354
                     /label="8901 - GR-CAN1.DS-fwd"
                     /note="sequence: ATATGACGTTTTATTACCTTTGATCACATTTCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    7322..7821
                     /label="CAN1 downstream flanks"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(7771..7814)
                     /label="E062 - GR-CAN1.DSflank-rvs"
                     /note="sequence: TTTGTTTTCTAATTTAGAATACTCACCTAACTCAGACATTATCG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          7782..7841
                     /label="8905 - GR-intBackbone-fwd_GO:CAN1.DS"
                     /note="sequence: GAGTTAGGTGAGTATTCTAAATTAGAAAACAAAGAGCTTAgaattcCCGTCGACCTCTAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(7784..7821)
                     /label="8902 - GR-CAN1.DS-rvs"
                     /note="sequence: TAAGCTCTTTGTTTTCTAATTTAGAATACTCACCTAAC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7822..7827
                     /label="EcoRI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(7863..7879)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     primer          complement(7876..7898)
                     /label=2864
                     /note="sequence: agcggataacaatttcacacagg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    7887..7903
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        complement(7911..7917)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7918..7935)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(7936..7941)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    7956..7977
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     rep_origin      complement(8265..8853)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(9024..9884)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     CDS             complement(9024..9884)
                     /label="Translation 9884-9024"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          9027..9054
                     /label="C692"
                     /note="sequence: ccaatgcttaatcagtgaggcacctatc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(9027..9054)
                     /label="C693"
                     /note="sequence: gataggtgcctcactgattaagcattgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(9054..9081)
                     /label="A541"
                     /note="sequence: ggatgaacgaaatagacagatcgctgag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          9233..9292
                     /label=3051
                     /note="sequence: GTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          9238..9257
                     /label=1533
                     /note="sequence: cctgcaactttatccgcctc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(9270..9289)
                     /label=3943
                     /note="sequence: TAGCTTCCCGGCAACAATTA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(9270..9292)
                     /label=4944
                     /note="sequence: ctctagcttcccggcaacaatta"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(9885..9989)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          9911..9940
                     /label="C694"
                     /note="sequence: GCATTTATCAGGGTTATTGTCTCATGAGCG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(9911..9940)
                     /label="C691"
                     /note="sequence: CGCTCATGAGACAATAACCCTGATAAATGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          9918..9939
                     /label=3172
                     /note="sequence: TCAGGGTTATTGTCTCATGAGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    9920..10020
                     /label="Sequenced on 6/25"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(9963..9989)
                     /label=5943
                     /note="sequence: cgcggaacccctatttgtttatttttc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG
       61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
      121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
      181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGgtgag
      241 tgaattcCAG TTCGAGTTTA TCATTATCAA TACTGCCATT TCAAAGAATA CGTAAATAAT
      301 TAATAGTAGT GATTTTCCTA ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT
      361 AACCCGTACA TGCCCAAAAT AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC
      421 TGGGTGAACA GTTTATTCCT GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG
      481 CATCCAGAAA AAAAAAGAAT CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT
      541 CATAGGTCCA TTCTCTTAGC GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG
      601 GCACAACCTC AATGGAGTGA TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA
      661 CCCACGCATG TATCTATCTC ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT
      721 TGGAAAAAGC TGAAAAAAAA GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT
      781 AATAAGTATA TAAAGACGGT AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT
      841 CTTAAATTCT ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAA GAACTTAGTT
      901 TCGAATAAAC ACACATAAAC AAACAAAatg aagaacatca agaagaacca agttatgaac
      961 ttgggtccaa actctaagtt gttgaaggaa tacaagtctc aattgatcga attgaacatc
     1021 gaacaattcg aagctggtat cggtttgatc ttgggtgacg cttacatccg atctcgcgac
     1081 gaaggtaaga cctactgtat gcaattcgaa tggaagaaca aggcttacat ggaccacgtt
     1141 tgtttgttgt acgaccaatg ggttttgtct ccaccacaca agaaggaaag agttaaccac
     1201 ttgggtaact tggttatcac ctggggtgct caaaccttca agcaccaagc tttcaacaag
     1261 ttggctaact tgttcatcgt taacaacaag aaaaccatcc caaacaactt ggttgaaaac
     1321 tacttgaccc caatgtcttt ggcttactgg ttcatggacg acggtggtaa gtgggactac
     1381 aacaagaact ctaccaacaa gtctatcgtt ttgaacaccc aatctttcac cttcgaagaa
     1441 gttgaatact tggttaaggg tttgagaaac aagttccaat tgaactgtta cgttaagatc
     1501 aacaagaaca agccaatcat ctacatcgac tctatgtctt acttgatctt ctacaacttg
     1561 atcaagccat acttgatccc acaaatgatg tacaagttgc caaacaccat ctcttctgaa
     1621 accttcttga agtaaataaa gcaatcttga tgaggataat gatttttttt tgaatataca
     1681 taaatactac cgtttttctg ctagattttg tgatgacgta aataagtaca tattactttt
     1741 taagccaaga caagattaag cattaacttt acccttttct ttctaagttt caatattagt
     1801 tatcactgtt taaaagttat ggcgagaacg tcggcggtta aaatatatta ccctgaacgg
     1861 aattcTATAT CTTTAACAGA TTCCAAACCC TAAAGTGTCC GAATTTTCAA TAGGGCGAAC
     1921 TTGAAGAATA ACCAAGGTCA ATAATATATC TTTTAGTATA ACCCTGAAAT TTGCCCTATA
     1981 GAAATCTAGG GTTTCTGTGT GGTTTCCGGG TGAGTCATAC GGCTTTTTTG AATTTCTTTT
     2041 TTTGCAGTTG TCTCTATCAA TGAAAATTTC GAGGAAGACG ATAAGGTTAA GATAAGTAGA
     2101 TAAGAGAATG ATACGAGATA AAGCACAAAT TAGCAGAAAG AAGAGTGGTT GCGAACAGAG
     2161 TAAACCGAAT CAGGGAATCC CTTTTTGCAA AAACATCAAT TATCCTTTTC TTTTTTTTAC
     2221 GTATATATCT GGAACAGAAA TATATAAGTT ACTATTATAC TTATAGTTGG ATCCAGTTTT
     2281 TAATCTGTCG TCAATCGAAA GTTTATTTCA GAGTTCTTCA GACTTCTTAA CTCCTGTAAA
     2341 AACAAAAAAA AAAAAAGGCA TAGCATCCGA GTGAGCAATT CAGGATACCG ACAGCAGTAG
     2401 TGTGGATAGA TTGTGCAAGA CTAGTAACTG GTAAAGGGCT CCCTGATAAG GCCTTGGAGT
     2461 GCTCCATGAT TCTCGGAGTC ATATTTGCCG TTTTATCATT AATCAGAAAC ACTTATAGAG
     2521 ATTACGGATA CGGGTGGATA TTATATATTC CGTCTGGTGT AGCAGTCGGT GTTGGTATAT
     2581 TTAATTCTCC CAGTTTTACA ATTGCAAGAT TCATCGGCGG GTGGGCTTCG CATTTTTGGT
     2641 TGAAGAATCA TAGGGGTGAC TTAAATGCGA AAACAAAAAT GATTGTATTC AGTTCGGGGT
     2701 TGGTCTTAGG TGAAGGTATC TTTAGCGTAA TAAACATGCT CTTCATCTGC TTAAATGTCC
     2761 CTCACTATTA GGTATATAAT ATTTTACTAA TAAAGAATTT ACAAAGTTTA CAAATTTTAA
     2821 ATTATTTACT CATCACATAA TTCGATGTCT TAGATTTCCC TGTATATATA TTTCTAGGTG
     2881 ACAAAAATCC TAGGCAAAAT GTTATATTAT ACGCGACCTT GAAATATCGG CTATCTTGGT
     2941 GTACAACATG GTCCATTTAA GAAAAATAAA AAAAAGTGAT GGGCACTGTT AAAACAGGCA
     3001 GAAGCCTCAT TCATAACTGC TGAAGGGTTA AGATCGTATA GGTAGGAACG ATCTGACCTC
     3061 TCATAatgga ctacaaagac aaagcattaa acgacttgag aaacgtatac gcagacttcg
     3121 actccttgcc attggacttc agacaaatct taattaagga tagagctaca ttgttgcaaa
     3181 aggaagacgt tgaaaagaaa attttggaaa gacaagaaga tgccaaaaag tacgctgaat
     3241 acttgaagca atcagaaatc ccagaaagaa tctccttacc taacatcaag agacataagg
     3301 gtgtatcaat ctcctttgaa gaaacatctg aagatatggt cttggaacca agacctttta
     3361 tcttcgacgg tttgaacatc agatgtttca gaagagaaac catattcagt ttgaaaaata
     3421 agattttgaa catggttaag gaatcttcat cctttaaaaa cgtttcaaga caaagtgtat
     3481 cttttatgta cttcaaaatt tttaataagg gtaaagttat tgcaagtaca aagtctgtta
     3541 acatctatga agataagatc gacgaaagat tggaagattt gtgtaacaac ttcgatgacg
     3601 tattgaaaaa gattatagac gtcacctatg gttacgaatc cttattcgtt agtgaaactt
     3661 actcttacgt aatattctat gctaaatcaa tctactttcc acaacctaga tgcgttaaca
     3721 actggggtaa caacatccca aacatcttga cattcgattc ctttaagctt ttcaccgcaa
     3781 acaaaaacaa cgttagttgt atcaagcaat gttcaagatt tttgtggcaa aaagatttca
     3841 acactttaga agaaatgata gaatacaaga acggtaatat ttgtatcgtc acaccacaat
     3901 tgcatatcaa cgatgttaga gacatcaagt cattcaacga tatcagattg tactcagaat
     3961 cccctattaa aacattttcc gttattgata acactatcac atatttgttt tacttcaagg
     4021 aacatttggg tgttattttt aacatcacta agtcaagaca tgatagaaga gtaacaaaat
     4081 tttcaccatt gtccaagttc agtgacgtca aaaatatcac tgtttgtttc gatatcgaaa
     4141 gttacttcga cccagaaaag gaatctaacc aagttaacat ccctttcatt tgttgcgctt
     4201 caatcatcta taataaggtt attggtaaca tcgtcgattt tgaaggtaga gactgtgtcg
     4261 cacaaatgat cgaatacgtt gtagatattt gcggtgaatt gaacataagt tctgttgaat
     4321 tgatcgccca taacggtggt ggttatgatt tccactacat cttgtcatcc atgtataatc
     4381 cagctgcaat taaaaacata ttgatcagaa acaactcttt tatttctttt aatttcgcac
     4441 atgatggtgt caagttctct gttaaggaca gttactcatt tttgtCgtgt agtttggcca
     4501 acgcttctaa agcattttta aacgaagGaa cttttaagaa aactgatttc cctcatcacg
     4561 acttgaaaac tgccgatgac ttatataagg tctacaagga atggagttct gttaacacag
     4621 aaattaacca tgtcgttgaa aaggaaaagt tgttgatcac ctctgaacac atcgttaact
     4681 tcaccaagaa cgataagagt aagactttga tcgaatggtc taaggattac tgtagaaacg
     4741 acgtattggt cttgtctaag gtttggttgg agtttaaaaa cgctgtagaa gatattttta
     4801 actgcgaatt ggtagaccaa accatgactt tggctggttt gtcatacaag ttgttccaag
     4861 caaacatgcc attcgatgtc gaattgagac atcctaataa ggaagactac ttcaacatga
     4921 gagaagcatt gattggtggt agatgtatct ctgttaacgg tatctacaGg gatgttttgt
     4981 gcttagacgt aaaaTCATTA TATCCAgctt ctatggcttt ctatgatcaa ccttacggtt
     5041 cttttaagag agtttcatcc agaccaaaag atgaattggg tatctattac gttagagtaa
     5101 ccAcaaacag aaacaacaag tcaaactttt tccctatcag atcccataat aagatcactt
     5161 acaacaactt cgaagaatca acttatattg cttggtacac aaacgttgat atTgacattg
     5221 gtttgtcaga aggtcacaac atcgaataca tccctttcga ttcctatggt aacattggtt
     5281 actcatggtc caaaaagggt aaaatttttg aaaagtacat caGggatgtt ttgtacaaat
     5341 tgaagataaa atatgaaaag caaaataaca aggtaaaaag aaatgtcatc aaaaAGatca
     5401 tgaactcatt gtggggtaaa ttcgcccaaa agtgggttaa cttcgaatac ttcatcaagt
     5461 cagaagatga cattgatttc gaatccgaag aagcatataa aatttgggat acagacttca
     5521 tgttgatcaa aaagattaag gaatctaTct acagttctaa gccaatacaa aacggtgttt
     5581 ttactttaag ttgggccaga tatcacatga agtctatttg ggatgcaggt gccaaagaag
     5641 gtgctgaatg tatctattca gatacagact ccatattcgt tcataaggaa cacttcaaaa
     5701 agaatgcaaa gtttatgttg aacggtttga aagtaccaat catcggttct gaagtcggtc
     5761 aatCTgaatt agaatgtgaa tttgataaat tgttgtgcgc cggtaaaaag caatacatgg
     5821 gtttctacac ttacttccaa gatggtaaac cttgcatcaa ggaaaagaaa agattcaaag
     5881 gtataccaag taactacatc atacctgaat tgtatgctca cttgttatct ggtgcagata
     5941 aggaagccaa aattcaatAt ttaaaattca gaagagaatg gggttccgtc aagggttaca
     6001 tagaaaataa gactgtcaaa gccacctaaC TCGAGGcgaa tttcttatga tttatgattt
     6061 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     6121 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     6181 aggtatagca tgaggtcgct cgacatggag gcccagaata ccctccttga cagtcttgac
     6241 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     6301 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     6361 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     6421 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     6481 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     6541 caaccatggg taccactctt gacgacacgg cttaccggta ccgcaccagt gtcccggggg
     6601 acgccgaggc catcgaggca ctggatgggt ccttcaccac cgacaccgtc ttccgcgtca
     6661 ccgccaccgg ggacggcttc accctgcggg aggtgccggt ggacccgccc ctgaccaagg
     6721 tgttccccga cgacgaatcg gacgacgaat cggacgacgg ggaggacggc gacccggact
     6781 cccggacgtt cgtcgcgtac ggggacgacg gcgacctggc gggcttcgtg gtcgtctcgt
     6841 actccggctg gaaccgccgg ctgaccgtcg aggacatcga ggtcgccccg gagcaccggg
     6901 ggcacggggt cgggcgcgcg ttgatggggc tcgcgacgga gttcgcccgc gagcggggcg
     6961 ccgggcacct ctggctggag gtcaccaacg tcaacgcacc ggcgatccac gcgtaccggc
     7021 ggatggggtt caccctctgc ggcctggaca ccgccctgta cgacggcacc gcctcggacg
     7081 gcgagcaggc gctctacatg agcatgccct gcccctaatc agtactgaca ataaaaagat
     7141 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     7201 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     7261 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     7321 gATATGACGT TTTATTACCT TTGATCACAT TTCCACGCCA TTTCGCATTC TCACCCTCAT
     7381 AAGTCATACA CCGAAAAGAA AGTTTAAGGG ATCAATGAGC TTACTATAAT CTCAGTATAT
     7441 TTATTTTTAT CGATGATTCA CCACAACAAT CTTGCTCCCG AAAAGAAAGC AGACGGAGTA
     7501 GAAGCATTTG AAACTCCTTC AGACCTTCAA GTATATATAT ATATATATAT ATATGTATAT
     7561 GTGTACATTT TCACGCTAAT ACTAATGTAT AATTAGAAGA TAATTTTTAC TCATTTTTCG
     7621 TTATCTTCAC GTCACCCGAA CCTAGAACCA AATGTCATTT TCACGATATG TAAATAGTGA
     7681 AATAGGCAAA AACGCCAAAA AGTAGTAAGC GCAACATACA CTAAACCATT AAAGAATATC
     7741 TCGACCAGAA TCTAACAGAT ATACATGTTC CGATAATGTC TGAGTTAGGT GAGTATTCTA
     7801 AATTAGAAAA CAAAGAGCTT AgaattcCCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA
     7861 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
     7921 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
     7981 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA
     8041 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC
     8101 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
     8161 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
     8221 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
     8281 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
     8341 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
     8401 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA
     8461 TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
     8521 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
     8581 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
     8641 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
     8701 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
     8761 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
     8821 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
     8881 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     8941 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     9001 TATGAGTAAA CTTGGTCTGA CAGttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9061 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9121 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
     9181 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9241 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9301 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9361 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9421 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9481 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9541 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9601 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9661 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9721 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9781 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9841 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatACTCTT CCTTTTTCAA
     9901 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     9961 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC
//