LOCUS       LHZ1494                 6369 bp ds-DNA     circular SYN 18-APR-2024
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, April 18, 2024 from SnapGene 3.2.1 to Vector 
            NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|994032000|
COMMENT     VNTDBDATE|1054944000|
COMMENT     VNTNAME|LHZ1494|
COMMENT     VNTAUTHORNAME|Triple Threat|
FEATURES             Location/Qualifiers
     promoter        8..386
                     /label=TEF Promoter
     CDS             387..1415
                     /codon_start=1
                     /label=HphMX4
     terminator      1416..1650
                     /label=TEF terminator
     misc_feature    1696..1828
                     /label=tRNA-gly
     misc_feature    1829..1849
                     /label=sg7RNA
     misc_RNA        1850..1925
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     terminator      1926..1945
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(1968..1974)
                     /label=T7 promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3040..3900)
                     /codon_start=1
                     /label=Lactamase-AmpC
     promoter        complement(3901..4005)
                     /label=AmpR promoter
     CDS             4392..5637
                     /codon_start=1
                     /label=ARS1
     misc_feature    5644..5776
                     /label=tRNA-gly
     misc_feature    5777..5797
                     /label=sg1RNA
     misc_RNA        5798..5873
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     terminator      5874..5893
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(5916..5934)
                     /label=T7 promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA 
                     polymerase"
     promoter        5948..6216
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_feature    6217..6237
                     /label=sg4RNA
     misc_RNA        6238..6313
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     terminator      6314..6333
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(6356..6362)
                     /label=T7 promoter
                     /note="T7 promoter promoter for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(6365..6369)
                     /label=M13F primer site
ORIGIN
        1 tctgtttagc ttgccttgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
       61 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
      121 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
      181 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
      241 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
      301 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
      361 cacatcacat ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc gcgacgtctg
      421 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
      481 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
      541 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
      601 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
      661 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
      721 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
      781 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
      841 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
      901 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
      961 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     1021 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     1081 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     1141 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     1201 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     1261 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     1321 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     1381 gacgccccag cactcgtccg agggcaaagg aataatcagt actgacaata aaaagattct
     1441 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     1501 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     1561 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     1621 gtcgattcga tactaacgcc gccatccagt gtcgaaaacg agctcgaatt catcgatgat
     1681 atcagatcca ctagtaattt ctcttctacc acgaactcga cactgtagtc tagtggttta
     1741 ggatactccc cttccactta acaagttgct tcctaaaaag aagtaacaag gcgagggagt
     1801 gacacgggtt cgaatcccgt cagtgtcagt acacagcatt ggaaataccg ttttagtact
     1861 ctggaaacag aatctactaa aacaaggcaa aatgccgtgt ttatctcgtc aacttgttgg
     1921 cgagattttt ttgtttttta tgtctgcggc cgcggtaccc aattcgccct atagggccta
     1981 tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt
     2041 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2461 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2701 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4021 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4081 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4141 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4201 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     4261 gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta
     4321 atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa cgcggccgcc
     4381 agctgaagct tatcgattga agttttgtcc aactatccac tatggatatg cgttttgttg
     4441 attaacctta aataacacgt atttcgcatt ttccaaaagc cttttttcat aactacaaac
     4501 taactatttt tgtttatttt acatcagtaa attatgcgca gaaaatatgt aaggctatat
     4561 actcaatata gtggaagagc ctctggctac ttaatctggg ttcatatatt ctgtcagtgg
     4621 tatagtaaat ttaaatagtg aatttgggcg catgtagaga gatcttttga ttaataaccc
     4681 agtatttgat tcttgaatgt tatttgtctc ttattaagta tttcagtttg atttatatat
     4741 tttgaaagta attgttgctt taccttccaa acaataaaaa aatataaaaa aatgtaaaaa
     4801 atataataaa tattaaataa aatactactt gtttgaaaat cagagaaaat ccaccaaaat
     4861 atcaatcatt tcaaggattt ccgaaccaag ttcgagatag tcctttaagg tcagtaaaat
     4921 tcagttgcac gtataacaga tatttttcat tttgttccaa tttaaaagtc ccccattttt
     4981 aaaattattg aaaataaaaa ttaaaaaatt aaaaggaatc tctctatgtc actttaaaat
     5041 aaataaattg aaaatgatat atcgttaaaa gtcaaccgca acaccaccta tttctaagag
     5101 gagagttcta aaaaaatcat agtaccacac aggtaaacta aaacagctaa attcaacata
     5161 atacctgtgg atataattac atacaaaaat ataattaaaa aaatacatta aaaataattt
     5221 atttttgtaa aacccataaa atatatttta ctttcggaac aactttttaa cttataattt
     5281 tgttttaaat aaaaacgttg tatttaaaaa taataaaata ttaagtaaaa atttaaagca
     5341 tatttattta aaatataaaa tactactaaa attactctaa acttcaaaat aaaaaaataa
     5401 taaattatat agttttaaaa ttaataattt gtatacacgt gaccagacca taatagtttt
     5461 cttttcttga aactgccatg aatttaatag atttttttta cacatattta cagtaagttt
     5521 tgtttttacg ttgaattaat attgtatacg tacttagaag gataacttcc aactatgaat
     5581 atgtaggtta aaaggtaaat agagaagcct tacttttatc agaaatgaaa ggagctcgtc
     5641 gacaatttct cttctaccac gaactcgaca ctgtagtcta gtggtttagg atactcccct
     5701 tccacttaac aagttgcttc ctaaaaagaa gtaacaaggc gagggagtga cacgggttcg
     5761 aatcccgtca gtgtcaaatt gggggaatta taagcgtgtt ttagtactct ggaaacagaa
     5821 tctactaaaa caaggcaaaa tgccgtgttt atctcgtcaa cttgttggcg agattttttt
     5881 gttttttatg tctgcggccg cggtacccaa ttcgccctat agtgagtcgt attagttaat
     5941 taacgcttct ttgaaaagat aatgtatgat tatgctttca ctcatattta tacagaaact
     6001 tgatgttttc tttcgagtat atacaaggtg attacatgta cgtttgaagt acaactctag
     6061 attttgtagt gccctcttgg gctagcggta aaggtgcgca ttttttcaca ccctacaatg
     6121 ttctgttcaa aagattttgg tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg
     6181 cgttcgaaac ttctccgcag tgaaagataa atgatctaga aaaacagtag tggaagggtt
     6241 ttagtactct ggaaacagaa tctactaaaa caaggcaaaa tgccgtgttt atctcgtcaa
     6301 cttgttggcg agattttttt gttttttatg tctgcggccg cggtacccaa ttcgccctat
     6361 aggcactgg
//
