LOCUS       pKDC.100                5547 bp    DNA     circular UNA 29-MAY-2024
DEFINITION  Yeast ncRNA + ribozymes backbone with PaqCI sites around 3' end of
            ncRNA.
ACCESSION   urn.local...16-d4vdn2g
VERSION     urn.local...16-d4vdn2g
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(5134..5547,1..90)
                     /standard_name="CEN/ARS"
     promoter        127..231
                     /standard_name="AmpR promoter"
     CDS             232..300
                     /standard_name="AmpR"
     CDS             301..1092
                     /standard_name="AmpR"
     rep_origin      1263..1851
     promoter        1907..2267
                     /standard_name="pGal7"
     misc_feature    2268..2314
                     /standard_name="HH-Ribozyme"
     misc_feature    2315
                     /standard_name="msr"
     misc_feature    2316..2330
                     /standard_name="crispey-ext"
     misc_feature    <2331..2411
                     /standard_name="msr"
     misc_feature    2331..2342
                     /standard_name="inverted repeat"
     misc_RNA        2401..>2423
                     /standard_name="msd"
     overhang        complement(2424..2427)
                     /created_by="katecrawford"
                     /label="4 bp overhang"
     motif           complement(2428..2434)
                     /created_by="katecrawford"
                     /label="PaqCI site"
     unsure          2435..2524
                     /created_by="katecrawford"
                     /modified_by="katecrawford"
                     /label="90 bp random spacer"
     motif           2525..2531
                     /created_by="katecrawford"
                     /label="PaqCI site"
     misc_feature    complement(<2534..2537)
                     /standard_name="linker grna"
     misc_feature    2536..2626
                     /standard_name="modified SpCas9 scaffold"
     misc_feature    2627..2697
                     /standard_name="HDV-Ribozyme"
     terminator      2698..3056
                     /standard_name="tGal7"
     promoter        complement(3070..3088)
                     /standard_name="T7_promoter"
     misc_feature    3100..3114
                     /standard_name="lacZ_a"
     CDS             3115
                     /standard_name="lacZα-Broken"
     CDS             3116..3476
                     /standard_name="lacZα"
     rep_origin      3259..3714
     CDS             complement(3845..4648)
                     /standard_name="URA3"
     promoter        complement(4649..4869)
                     /standard_name="URA3 promoter"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaaggg tgcgcatctg
     2281 atgagtccgt gaggacgaaa cgagctagct cgtcatgata agattccgta tgcgcaccct
     2341 tagcgagagg tttatcatta aggtcaacct ctggatgttg tttcggcatc ctgcattgaa
     2401 tctgagttac tgtctgtttt ccttgatgca ggtgtagctt gatgcggaag gagatctgga
     2461 atgaactggt ctatgtgaca gaaactgtgc aagtacctaa tctcgttagt gtaggttcta
     2521 gctacacctg catcagtttc agagctatgc tggaaacagc atagcaagtt gaaataaggc
     2581 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttgatg gccggcatgg
     2641 tcccagcctc ctcgctggcg ccggctgggc aacaccttcg ggtggcgaat gggacttaaa
     2701 gaaagtggaa tattcattca tatcatattt tttctattaa ctgcctggtt tcttttaaat
     2761 tttttattgg ttgtcgactt gaacggagtg acaatatata tatatatata tttaataatg
     2821 acatcattat ctgtaaatct gattcttaat gctattctag ttatgtaaga gtggtccttt
     2881 ccataaaaaa aaaaaaaaag aaaaaagaat tttaggaata caatgcagct tgtaagtaaa
     2941 atctggaata ttcatatcgc cacaacttct tatgcttata aaagcactaa tgcctgaatt
     3001 tatgttgaaa atatgtgtca caaataaaga aactgtgaca tctgacacat ttccacgtac
     3061 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     3121 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3181 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3241 tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3301 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3421 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3481 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3541 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3601 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3661 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat
     3721 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt aataactgat
     3781 ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag
     3841 ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac
     3901 gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa
     3961 tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc
     4021 ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc
     4081 cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt
     4141 caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta
     4201 acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa
     4261 caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt
     4321 atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt
     4381 cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca
     4441 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
     4501 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
     4561 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
     4621 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc
     4681 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
     4741 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
     4801 aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
     4861 tgaattgaaa agctgtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4921 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4981 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5041 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5101 ttaatgtcat gataataatg gtttcttagg acggatcgct tgcctgtaac ttacacgcgc
     5161 ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg
     5221 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa
     5281 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat
     5341 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa
     5401 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata
     5461 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt
     5521 ttacatcttc ggaaaacaaa aactatt
//
