LOCUS       pSCL195                10936 bp    DNA     circular UNA 29-MAY-2024
DEFINITION  site2.
ACCESSION   urn.local...91-dups8d7
VERSION     urn.local...91-dups8d7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    42..242
                     /standard_name="dubious DED1 ORF"
     rep_origin      433..739
     promoter        927..945
                     /standard_name="T7_promoter"
     terminator      954..1178
                     /standard_name="Gal10 terminator"
     misc_feature    1179..2165
                     /standard_name="RT Ec86 Sc optimized"
     misc_feature    2139..2162
                     /standard_name="SV40 NLS"
     promoter        2166..2398
                     /standard_name="Gal10p"
     promoter        2399..2829
                     /standard_name="Gal1/10 promoter"
     CDS             2855..6994
                     /standard_name="Cas9"
     misc_feature    7006..7252
                     /standard_name="CYC1 Terminator"
     misc_feature    7722..8310
                     /standard_name="ori"
     gene            complement(8481..9341)
                     /standard_name="Ampicillin"
     site            9498..9725
                     /standard_name="site2"
     misc_feature    10001..10317
                     /standard_name="HIS3 promoter"
     gene            10318..38
                     /standard_name="HIS3"
     nc              10623..10809
                     /standard_name="deletion in by4742"
ORIGIN      
        1 gtaccaacga tgttccctcc accaaaggtg ttcttatgta gtgacaccga ttatttaaag
       61 ctgcagcata cgatatatat acatgtgtat atatgtatac ctatgaatgt cagtaagtat
      121 gtatacgaac agtatgatac tgaagatgac aaggtaatgc atcattctat acgtgtcatt
      181 ctgaacgagg cgcgctttcc ttttttcttt ttgctttttc tttttttttc tcttgaactc
      241 gacggatcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      301 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
      361 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      421 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      481 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      541 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      601 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      661 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      721 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc
      781 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      841 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      901 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgcatatct
      961 ttccatagat tttcaatttt tgatgtctcc atggtggtac agagaacttg taaacaattc
     1021 ggtccctaca tgtgaggaaa ttcgctgtga cacttttatc actgaactcc aaatttaaaa
     1081 aatagcataa aattcgttat acagcaaatc tatgtgttgc aattaagaac taaaagatat
     1141 agagtgcata ttttcaagaa ggatagtaag ctggcaaatc aagtcttagc cttgttcaat
     1201 gggttcttac cgtacttctt ttccaactta gagatgtagg taatcaatct tctgtgggac
     1261 ttagaatcaa cggacaagat gaaagacaac caacctctaa catgttcgat ttcggaggat
     1321 ttaccgcaga aaatatggtg gatcttggct ctgatttctt tgtacttttc tctaccaata
     1381 ccaacctttt cttgggagat aaccaaaccg gtaacttttc tttgggatct tggaccagaa
     1441 atacaggtct ttttggagtt gatgaccaaa ccttctgatg gaataatgga gaacaagaag
     1501 tctctggcct taacaacttt cttcatagat tgagcagaca aggtcaagtc atcagcgtat
     1561 ctagtgtata tcaaacctct agaaccagca taaccttgaa ttctgtagtc caacttggag
     1621 caaatcaagt tagccaattt tggagaagat ggagcacctt gtggcaacaa gttcttgtag
     1681 cagcaaatct tggtcaaaac agaggagatc aatctgttgt aacccaaaga atggaaaaca
     1741 ccgaaaacct tgttagcagt caaagatggg aagaaatctt ccaagtcgat gttcaaaatg
     1801 aagttggcac caatatgtgg agtggcattg ttcaagatgg attggtgctt ttcaaaaccg
     1861 atagaaaatg gggaggagga taacttgtcc aagatgtttc tcaaaaccca accttgtaaa
     1921 gccttcaatt ctctggatgg ttggtagata gttctcattc tcttttctgg acccttcttt
     1981 tcaacagtgt agattctgta tctgaaatcg gcggtgtaga tcaataatct caaggtttca
     2041 acagaaattc tggtagcctt agacatatcg tgcaagttgt tcatgactgg caaacccaaa
     2101 tttctcaatc tgaaggtgtt caagtattca gcggacttga cctttctttt cttctttggt
     2161 ggcatttata ttgaattttc aaaaattctt actttttttt tggatggacg caaagaagtt
     2221 taataatcat attacatggc attaccacca tatacatatc catatctaat cttacttata
     2281 tgttgtggaa atgtaaagag ccccattatc ttagcctaaa aaaaccttct ctttggaact
     2341 ttcagtaata cgcttaactg ctcattgcta tattgaagta cggattagaa gccgccgagc
     2401 gggcgacagc cctccgacgg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     2461 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     2521 tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa
     2581 ttaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc
     2641 tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatggaaa
     2701 agctgcataa ccactttaac taatactttc aacattttca gtttgtatta cttcttattc
     2761 aaatgtcata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
     2821 agaaaaaaca actagtggat cccccgggaa aaaaatggac aagaagtact ccattgggct
     2881 cgatatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag
     2941 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg
     3001 cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg
     3061 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga
     3121 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga
     3181 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca
     3241 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc
     3301 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct
     3361 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt
     3421 tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa
     3481 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct
     3541 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac
     3601 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga
     3661 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct
     3721 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa
     3781 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca
     3841 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga
     3901 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca
     3961 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct
     4021 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag
     4081 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt
     4141 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc
     4201 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc
     4261 agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca
     4321 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc
     4381 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata
     4441 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt
     4501 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt
     4561 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc
     4621 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa
     4681 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag
     4741 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa
     4801 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg
     4861 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc
     4921 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca
     4981 gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg
     5041 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa
     5101 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac
     5161 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa
     5221 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga
     5281 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga
     5341 catcaatcgg ctctccgact acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga
     5401 tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa
     5461 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc
     5521 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc
     5581 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa
     5641 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact
     5701 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga
     5761 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct
     5821 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt
     5881 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat
     5941 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga
     6001 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac
     6061 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat
     6121 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag
     6181 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa
     6241 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt
     6301 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat
     6361 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga
     6421 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg
     6481 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc
     6541 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc
     6601 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat
     6661 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa
     6721 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat
     6781 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac
     6841 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca
     6901 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag
     6961 cagggctgac cccaagaaga agaggaaggt gtgatctctt ctcgagtcat gtaattagtt
     7021 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     7081 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     7141 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     7201 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttcaccgcgg
     7261 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     7321 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc
     7381 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg
     7441 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7501 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7561 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7621 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7681 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7741 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7801 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7861 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7921 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7981 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8041 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8101 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8161 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8221 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8281 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8341 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8401 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8461 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8521 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8581 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8641 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8701 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8761 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8821 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8881 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8941 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9001 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9061 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9121 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9181 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9241 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9301 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9361 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9421 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtacct
     9481 ctgacacatg cagctcctaa cactcacagt gaaagaaatt acagtcgaaa cgtatcttaa
     9541 accatacctg catcagcaag taattcgagc ccgtgaatca ccgacttgct gaaactaacg
     9601 gtcagcagga cttgcggtcg acaggatgag cggttgtgtc gcatacgagt gctaaattcc
     9661 cctcactgtt acccagcgct acctcaatag attagcttat aagcacacct tgtttcacac
     9721 acctccggag acggtcacag cttgtacgtc taagaaacca ttattatcat gacattaacc
     9781 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     9841 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     9901 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     9961 tatgcggcat cagagcagat tgtactgaga gtgcaccata aattcccgtt ttaagagctt
    10021 ggtgagcgct aggagtcact gccaggtatc gtttgaacac ggcattagtc agggaagtca
    10081 taacacagtc ctttcccgca attttctttt tctattactc ttggcctcct ctagtacact
    10141 ctatattttt ttatgcctcg gtaatgattt tcattttttt ttttccccta gcggatgact
    10201 cttttttttt cttagcgatt ggcattatca cataatgaat tatacattat ataaagtaat
    10261 gtgatttctt cgaagaatat actaaaaaat gagcaggcaa gataaacgaa ggcaaagatg
    10321 acagagcaga aagccctagt aaagcgtatt acaaatgaaa ccaagattca gattgcgatc
    10381 tctttaaagg gtggtcccct agcgatagag cactcgatct tcccagaaaa agaggcagaa
    10441 gcagtagcag aacaggccac acaatcgcaa gtgattaacg tccacacagg tatagggttt
    10501 ctggaccata tgatacatgc tctggccaag cattccggct ggtcgctaat cgttgagtgc
    10561 attggtgact tacacataga cgaccatcac accactgaag actgcgggat tgctctcggt
    10621 caagctttta aagaggccct actggcgcgt ggagtaaaaa ggtttggatc aggatttgcg
    10681 cctttggatg aggcactttc cagagcggtg gtagatcttt cgaacaggcc gtacgcagtt
    10741 gtcgaacttg gtttgcaaag ggagaaagta ggagatctct cttgcgagat gatcccgcat
    10801 tttcttgaaa gctttgcaga ggctagcaga attaccctcc acgttgattg tctgcgaggc
    10861 aagaatgatc atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa
    10921 gccacctcgc ccaatg
//
