LOCUS       pET22b-GST-Kif1C        7719 bp DNA     circular SYN 31-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Ligation of : pET22b-HsCLASP2_EBBD-eGFP-6xHis.xdna [6107  nt] : 
            (SalI[5638] / NdeI[5202]) to PCR (NdeI-Kif1C-T1-SalI).xdna [1606  
            nt] : (NdeI[6] / SalI[1615])
FEATURES             Location/Qualifiers
     source          1..7719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=M13 origin
                     /note="color: #4682b4"
     rep_origin      complement(17..457)
                     /direction=LEFT
                     /label=F1 ori
                     /note="color: #4682b4"
     promoter        529..557
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             797..1456
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      1608..2236
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     misc_feature    complement(3648..4739)
                     /label=lacI
                     /note="color: #4682b4; direction: LEFT"
     primer_bind     5117..5136
                     /label=T7
                     /note="color: #008b8b; direction: RIGHT"
     misc_binding    5136..5158
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     CDS             5205..5858
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /note="color: #cc99b2"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     5206..6811
                     /note="note=""Fragment extracted from PCR 
                     (NdeI-Kif1C-T1-SalI).xdna [1606  nt] : (NdeI[6] / 
                     SalI[1615])"
                     /note="color: #84a4c0"
     CDS             5865..5888
                     /label=PreScission site
                     /note="color: #008000; direction: RIGHT"
     misc_feature    5897..5961
                     /label=insert from BamHI-TandemTEV-EcoRI Adaptor
                     /note="note=""Fragment extracted from BamHI-TandemTEV-EcoRI
                     Adaptor [65  nt] : (Uncut 5'[0] / Uncut 3'[65])"
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             5898..5918
                     /label=TEV site
                     /note="color: #008000; direction: RIGHT"
     CDS             5940..5960
                     /label=TEV site
                     /note="color: #008000; direction: RIGHT"
     misc_feature    5967..6809
                     /label=KIF1C(642-922)
                     /note="color: #ff9900; direction: RIGHT"
     CDS             6831..7544
                     /label=EGFP
                     /note="color: #32cd32; direction: RIGHT"
     CDS             7563..7580
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     terminator      7647..7694
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcc cctatactag
     5221 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag
     5281 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt
     5341 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa
     5401 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc
     5461 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg
     5521 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc
     5581 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg
     5641 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg
     5701 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta
     5761 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct
     5821 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggaa gttctgttcc
     5881 aggggcccct gggatccgag aacctctact tccaaggcga ctatgacatc ccaactacag
     5941 agaatctcta tttccagggt gaattcgaaa tggagaagag gctgcaggat ctggagaatc
     6001 agtaccggaa agaaaaggaa gaagccgatc ttctgctgga gcagcagcga ctgtatgcag
     6061 actcggacag cggggatgac tctgacaagc gctcttgtga agagagctgg aggctcatct
     6121 cctccttgcg ggagcagctg ccgcccacca cggtccagac cattgtcaaa cgctgtggtc
     6181 tgcccagcag tggcaagcgc agggcccctc gcagggttta tcagatcccc cagcgacgca
     6241 ggctgcaggg caaagacccc cgctgggcca ccatggctga cctgaagatg caggcggtga
     6301 aggagatctg ctacgaggtg gccctggctg acttccgcca cgggcgggct gagattgagg
     6361 ccctggccgc cctcaagatg cgggagctgt gtcgcaccta tggcaagcca gacggccccg
     6421 gagacgcctg gagggctgtg gcccgggatg tctgggacac tgtaggcgag gaggaaggag
     6481 gtggagctgg cagtggtggt ggcagtgagg agggagcccg aggggcggag gtggaggacc
     6541 tccgggccca catcgacaag ctgacgggga ttctgcagga ggtgaagctg cagaacagca
     6601 gcaaggaccg ggagctgcag gccctgcggg accgcatgct ccgcatggag agggtcatcc
     6661 ccctggccca ggatcatgag gatgagaatg aagaaggtgg tgaggtcccc tgggccccgc
     6721 ctgaaggatc agaggcagca gaggaggcag cccccagtga ccgcatgccg tcagcccggc
     6781 ccccctcgcc accactgtca agctgggagg gtcgactgaa ttccaccatg gtgagcaagg
     6841 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     6901 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     6961 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     7021 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     7081 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     7141 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     7201 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     7261 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     7321 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     7381 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     7441 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     7501 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgcg gccgcactcg
     7561 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     7621 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     7681 tgaggggttt tttgctgaaa ggaggaacta tatccggat
//
