LOCUS       pMSCVneo                8155 bp    DNA     circular SYN 03-Oct-2017
DEFINITION  .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
            Catalog Nos. 631461, 634401
FEATURES             Location/Qualifiers
     LTR             1..515
                     /label="5' MMSV LTR"
     misc_feature    516..1403
                     /label="Extended packaging signal"
     frag            1405..3141
                     /date="29-Sep-2019"
                     /note="1 to 1737 of MSCVneo-HA-ER-HoxB8-EcoR1-Xho1"
     misc_feature    1406
                     /note="EcoR1"
     misc_feature    1417..1449
                     /note="HA-tag"
     misc_feature    1450..1455
                     /note="MLU1"
     CDS             1456..2397
                     /note="huER-HBD(282-595)G400V"
     misc_feature    1810..1812
                     /note="GLY-->VAL Mutation"
     misc_feature    2398..2403
                     /note="MLU1"
     CDS             2404..3129
                     /note="Hoxb8"
     misc_feature    3130..3135
                     /note="Double-STOP"
     misc_feature    3136..3141
                     /note="XHO1"
     misc_feature    <3142..3147
                     /note="MCS"
     promoter        3157..3656
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3670..4473
                     /label="NeoR"
     LTR             4578..5061
                     /label="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     rep_origin      complement(5602..6190)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6361..7221)
                     /label="AmpR"
     promoter        complement(7222..7326)
                     /note="AmpR promoter"
BASE COUNT  1859 A  2258 C  2192 G  1846 T  0 OTHER
ORIGIN
        1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
       61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
      121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
      181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
      241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC
      301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC
      361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA
      421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA
      481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA
      541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG
      601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT
      661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
      721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
      781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
      841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
      901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
      961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
     1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
     1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
     1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
     1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
     1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
     1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
     1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTC GCCACCATGG GATACCCATA CGATGTTCCG
     1441 GATTACGCTA CGCGTTCTGC TGGAGACATG AGAGCTGCCA ACCTTTGGCC AAGCCCGCTC
     1501 ATGATCAAAC GCTCTAAGAA GAACAGCCTG GCCTTGTCCC TGACGGCCGA CCAGATGGTC
     1561 AGTGCCTTGT TGGATGCTGA GCCCCCCATA CTCTATTCCG AGTATGATCC TACCAGACCC
     1621 TTCAGTGAAG CTTCGATGAT GGGCTTACTG ACCAACCTGG CAGACAGGGA GCTGGTTCAC
     1681 ATGATCAACT GGGCGAAGAG GGTGCCAGGC TTTGTGGATT TGACCCTCCA TGATCAGGTC
     1741 CACCTTCTAG AATGTGCCTG GCTAGAGATC CTGATGATTG GTCTCGTCTG GCGCTCCATG
     1801 GAGCACCCAG TGAAGCTACT GTTTGCTCCT AACTTGCTCT TGGACAGGAA CCAGGGAAAA
     1861 TGTGTAGAGG GCATGGTGGA GATCTTCGAC ATGCTGCTGG CTACATCATC TCGGTTCCGC
     1921 ATGATGAATC TGCAGGGAGA GGAGTTTGTG TGCCTCAAAT CTATTATTTT GCTTAATTCT
     1981 GGAGTGTACA CATTTCTGTC CAGCACCCTG AAGTCTCTGG AAGAGAAGGA CCATATCCAC
     2041 CGAGTCCTGG ACAAGATCAC AGACACTTTG ATCCACCTGA TGGCCAAGGC AGGCCTGACC
     2101 CTGCAGCAGC AGCACCAGCG GCTGGCCCAG CTCCTCCTCA TCCTCTCCCA CATCAGGCAC
     2161 ATGAGTAACA AAGGCATGGA GCATCTGTAC AGCATGAAGT GCAAGAACGT GGTGCCCCTC
     2221 TATGACCTGC TGCTGGAGAT GCTGGACGCC CACCGCCTAC ATGCGCCCAC TAGCCGTGGA
     2281 GGGGCATCCG TGGAGGAGAC GGACCAAAGC CACTTGGCCA CTGCGGGCTC TACTTCATCG
     2341 CATTCCTTGC AAAAGTATTA CATCACGGGG GAGGCAGAGG GTTTCCCTGC CACAGTCACG
     2401 CGTGGAAGCT CTTATTTCGT CAACTCACTG TTCTCCAAAT ACAAAACCGG GGAGTCCCTG
     2461 CGCCCCAATT ATTATGACTG CGGCTTCGCC CAGGACCTGG GCGGCCGACC CACCGTGGTG
     2521 TACGGTCCCA GCAGCGGCGG CAGCTTCCAG CACCCTTCGC AAATCCAGGA GTTCTACCAC
     2581 GGGCCATCGT CGCTGTCCAC AGCTCCCTAC CAGCAGAACC CGTGCGCCGT GGCGTGCCAC
     2641 GGCGACCCCG GCAACTTCTA CGGCTACGAC CCTCTGCAGC GCCAGAGCCT GTTCGGTGCG
     2701 CAGGATCCAG ACCTGGTGCA GTACGCAGAC TGCAAGCTCG CGGCAGCCAG CGGCCTGGGC
     2761 GAGGAGGCCG AGGGGTCTGA GCAGAGCCCG TCGCCCACAC AGCTCTTTCC CTGGATGCGC
     2821 CCTCAAGCCG CCGGACGCAG GCGAGGCCGC CAGACCTACA GTCGCTACCA GACCCTGGAG
     2881 CTGGAGAAGG AGTTCCTATT TAATCCCTAT CTGACTCGCA AGCGGAGGAT CGAGGTATCG
     2941 CACGCGCTGG GACTGACAGA GAGACAGGTC AAAATCTGGT TCCAGAATCG GAGAATGAAG
     3001 TGGAAAAAGG AGAACAACAA AGACAAGTTT CCCAGCAGTA AATGCGAGCA GGAGGAGCTG
     3061 GAGAAAGAGA AGCTGGAGCG GGCACCAGAG ACCGCCGAGC AGGGCGATGC GCAGAAGGGT
     3121 GACAAGAAGT AGTAACTCGA GAGATCTAAT TCTACCGGGT AGGGGAGGCG CTTTTCCCAA
     3181 GGCAGTCTGG AGCATGCGCT TTAGCAGCCC CGCTGGGCAC TTGGCGCTAC ACAAGTGGCC
     3241 TCTGGCCTCG CACACATTCC ACATCCACCG GTAGGCGCCA ACCGGCTCCG TTCTTTGGTG
     3301 GCCCCTTCGC GCCACCTTCT ACTCCTCCCC TAGTCAGGAA GTTCCCCCCC GCCCCGCAGC
     3361 TCGCGTCGTG CAGGACGTGA CAAATGGAAG TAGCACGTCT CACTAGTCTC GTGCAGATGG
     3421 ACAGCACCGC TGAGCAATGG AAGCGGGTAG GCCTTTGGGG CAGCGGCCAA TAGCAGCTTT
     3481 GCTCCTTCGC TTTCTGGGCT CAGAGGCTGG GAAGGGGTGG GTCCGGGGGC GGGCTCAGGG
     3541 GCGGGCTCAG GGGCGGGGCG GGCGCCCGAA GGTCCTCCGG AGGCCCGGCA TTCTGCACGC
     3601 TTCAAAAGCG CACGTCTGCC GCGCTGTTCT CCTCTTCCTC ATCTCCGGGC CTTTCGACCT
     3661 GCAGCCAATA TGGGATCGGC CATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
     3721 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
     3781 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
     3841 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
     3901 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
     3961 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
     4021 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
     4081 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
     4141 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
     4201 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
     4261 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG
     4321 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
     4381 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC
     4441 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGGGGATC CGTCGACCTG CAGCCAAGCT
     4501 TATCGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA
     4561 CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAATACATA
     4621 ACTGAGAATA GAGAAGTTCA GATCAAGGTT AGGAACAGAG AGACAGCAGA ATATGGGCCA
     4681 AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC
     4741 CAGATGCGGT CCCGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC
     4801 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC
     4861 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC
     4921 GCCAGTCCTC CGATAGACTG CGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA
     4981 GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA
     5041 CCCGTCAGCG GGGGTCTTTC ATGGGTAACA GTTTCTTGAA GTTGGAGAAC AACATTCTGA
     5101 GGGTAGGAGT CGAATATTAA GTAATCCTGA CTCAATTAGC CACTGTTTTG AATCCACATA
     5161 CTCCAATACT CCTGAAATAG TTCATTATGG ACAGCGCAGA AAGAGCTGGG GAGAATTGTG
     5221 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     5281 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     5341 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
     5401 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
     5461 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
     5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     6121 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     6181 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     6241 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     6301 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     6361 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     6421 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     6481 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     6541 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     6601 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     6661 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     6721 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     6781 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     6841 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     6901 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     6961 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     7021 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     7081 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     7141 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     7201 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     7261 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     7321 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC
     7381 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA
     7441 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA
     7501 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG
     7561 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT
     7621 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG
     7681 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
     7741 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
     7801 TAAAACGACG GCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC
     7861 ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC
     7921 CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG
     7981 GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGCGATTAG TCCAATTTGT TAAAGACAGG
     8041 ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA GCCTATAGAG
     8101 TACGAGCCAT AGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAA
//
