LOCUS       Str-KDEL_CD63-SBP-mCherry.xdna    6635 bp      DNA     circular   06/06/2024
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2538..2659
                     /label=CD63
                     /SerialCloner_Color=&h00AC27C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4478..4498)
                     /label=M13-rev
                     /SerialCloner_Color=&h00008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     867..886
                     /label=T7
                     /SerialCloner_Color=&h00008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(4866..5494)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h004682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(4504..4526)
                     /label=LacO
                     /SerialCloner_Color=&h006495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(5646..6305)
                     /label=AmpR
                     /SerialCloner_Color=&h008FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1081..1557
                     /label=Core Streptavidin
                     /SerialCloner_Color=&h000000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1567..1578
                     /label=KDEL
                     /SerialCloner_Color=&h00C0066E
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3524..4129
                     /label=CD63
                     /SerialCloner_Color=&h00981FC0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6545..6573)
                     /label=Amp prom
                     /SerialCloner_Color=&h004682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4531..4560)
                     /label=lac
                     /SerialCloner_Color=&h004682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     intron          1614..1909
                     /label=synth int
                     /SerialCloner_Color=&h004682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2666..2776
                     /label=SBP tag
                     /SerialCloner_Color=&h00008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2785..3490
                     /label=mCherry
                     /SerialCloner_Color=&h00C01212
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT 
       61 GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG 
      121 TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT 
      181 CTGCTTAGGG TTAGGCGTTT TGCGCTGCTT CGCGATGTAC GGGCCAGATA TACGCGTTGA 
      241 CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA 
      301 TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC 
      361 GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT 
      421 TTCCATTGAC GTCAATGGGT GGACTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA 
      481 GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG 
      541 CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA 
      601 GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG 
      661 TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG 
      721 CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG 
      781 GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTCTCTGGCT AACTAGAGAA 
      841 CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT ATAGGGAGAC CCAAGCTTGG 
      901 TACCGAGCTC GGATCGATAT CTGCGGCCTA GCTAGCGCTT AAGGCCTGTT AAccggtATG 
      961 GATGTATGCG TCCGTCTTGC CCTGTGGCTC CTCTGGGGAC TCCTCCTGCA CCAGGGCCAG 
     1021 AGCCTCAGCC ATAGTCACAG TGAGAAGGCG ACAGGAACCA GCTCGGGCGC Cggtttaaac 
     1081 GACCCTAGCA AAGACTCAaa agCTCAGGTG TCCGCTGCCG AGGCTGGCAT TACTGGAACA 
     1141 TGGTACAATC AGCTCGGGAG CACCTTTATT GTGACTGCTG GAGCCGATGG AGCCCTCACC 
     1201 GGAACATACG AATCTGCTGT GGGAAACGCC GAATCACGGT ACGTCCTCAC TGGCCGATAC 
     1261 GATAGTGCCC CTGCCACCGA CGGATCTGGG ACTGCCCTGG GATGGACTGT CGCTTGGAAA 
     1321 AACAACTACC GGAATGCTCA TTCTGCCACA ACATGGAGTG GACAGTACGT GGGAGGCGCT 
     1381 GAGGCTAGAA TCAATACACA GTGGCTGCTC ACATCTGGCA CAACCGAGGC AAATGCTTGG 
     1441 AAATCCACCC TGGTGGGACA TGACACATTC ACCAAAGTGA AACCCTCCGC CGCTTCAATC 
     1501 GATGCCGCCA AAAAAGCCGG AGTCAACAAC GGCAATCCTC TGGATGCCGT CCAGCAGctc 
     1561 ggaccgAAAG ATGAACTGTG AgcggccGCA TAGATAACTG ATCCAGTGTG CTGGAATTAA 
     1621 TTCGCTGTCT GCGAGGGCCA GCTGTTGGGG TGAGTACTCC CTCTCAAAAG CGGGCATGAC 
     1681 TTCTGCGCTA AGATTGTCAG TTTCCAAAAA CGAGGAGGAT TTGATATTCA CCTGGCCCGC 
     1741 GGTGATGCCT TTGAGGGTGG CCGCGTCCAT CTGGTCAGAA AAGACAATCT TTTTGTTGTC 
     1801 AAGCTTGAGG TGTGGCAGGC TTGAGATCTG GCCATACACT TGAGTGACAA TGACATCCAC 
     1861 TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGGTCCAAC TGCAGGTCGA GCATGCATCT 
     1921 AGGGCGGCCA ATTCCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT 
     1981 TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG 
     2041 GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT 
     2101 CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG 
     2161 AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC 
     2221 CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG 
     2281 CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT 
     2341 CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG 
     2401 ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG 
     2461 GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA 
     2521 ACccgggaGG cgcgccATGG CGGTGGAAGG AGGAATGAAA TGTGTGAAGT TCTTGCTCTA 
     2581 CGTCCTCCTG CTGGCCTTTT GCGCCTGTGC AGTGGGACTG ATTGCCGTGG GTGTCGGGGC 
     2641 ACAGAGATCT CTAGCCACCg aattcGACGA GAAGACCACT GGTTGGCGAG GTGGACACGT 
     2701 TGTTGAAGGA CTGGCTGGGG AACTTGAACA ACTTCGTGCA CGACTGGAGC ATCACCCACA 
     2761 AGGTCAACGT GAACCAcctg caggtGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT 
     2821 CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA 
     2881 GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT 
     2941 GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG 
     3001 CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC 
     3061 CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC 
     3121 CCAGGACTCC TCCCTaCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA 
     3181 CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA 
     3241 GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA 
     3301 GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA 
     3361 GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA 
     3421 CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA 
     3481 GCTGTACAAG ggccggccaA CCGGTGGAGC TCGAATCAGA TCTCTTGTCC TGAGTCAGAC 
     3541 CATAATCCAG GGGGCTACCC CTGGCTCTCT GTTGCCAGTG GTCATCATCG CAGTGGGTGT 
     3601 CTTCCTCTTC CTGGTGGCTT TTGTGGGCTG CTGCGGGGCC TGCAAGGAGA ACTATTGTCT 
     3661 TATGATCACG TTTGCCATCT TTCTGTCTCT TATCATGTTG GTGGAGGTGG CCGCAGCCAT 
     3721 TGCTGGCTAT GTGTTTAGAG ATAAGGTGAT GTCAGAGTTT AATAACAACT TCCGGCAGCA 
     3781 GATGGAGAAT TACCCGAAAA ACAACCACAC TGCTTCGATC CTGGACAGGA TGCAGGCAGA 
     3841 TTTTAAGTGC TGTGGGGCTG CTAACTACAC AGATTGGGAG AAAATCCCTT CCATGTCGAA 
     3901 GAACCGAGTC CCCGACTCCT GCTGCATTAA TGTTACTGTG GGCTGTGGGA TTAATTTCAA 
     3961 CGAGAAGGCG ATCCATAAGG AGGGCTGTGT GGAGAAGATT GGGGGCTGGC TGAGGAAAAA 
     4021 TGTGCTGGTG GTAGCTGCAG CAGCCCTTGG AATTGCTTTT GTCGAGGTTT TGGGAATTGT 
     4081 CTTTGCCTGC TGCCTCGTGA AGAGTATCAG AAGTGGCTAC GAGGTGATGT AGttaatTAA 
     4141 ctctagATAA CTGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA 
     4201 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA 
     4261 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 
     4321 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 
     4381 ACGCGTCGAG TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC 
     4441 TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG 
     4501 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA 
     4561 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT 
     4621 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA 
     4681 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 
     4741 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 
     4801 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 
     4861 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 
     4921 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 
     4981 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 
     5041 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC 
     5101 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 
     5161 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 
     5221 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 
     5281 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 
     5341 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 
     5401 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 
     5461 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 
     5521 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 
     5581 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 
     5641 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 
     5701 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 
     5761 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 
     5821 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 
     5881 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 
     5941 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 
     6001 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 
     6061 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 
     6121 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 
     6181 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 
     6241 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 
     6301 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 
     6361 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 
     6421 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 
     6481 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6541 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6601 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGA
//