LOCUS       evoBxb1                 5801 bp    DNA     circular UNK 04-JUN-2024
DEFINITION  .
ACCESSION   <unknown
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             771..825
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.078485687903971"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     promoter        complement(841..938)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="96.07843137254902"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     promoter        1051..1081
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lac UV5 promoter"
     protein_bind    complement(1085..1107)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     enhancer        1135..1438
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
                     /label="CMV enhancer"
     promoter        1439..1642
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="CMV promoter"
     promoter        1736..1757
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.5830618892508146"
                     /fragment="True"
                     /other="promoter"
                     /label="SR-alpha promoter"
     promoter        1758..1776
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="SP6 promoter"
     intron          3355..3469
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="33.23699421965318"
                     /fragment="True"
                     /other="intron"
                     /label="large T intron"
     intron          complement(3539..3568)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="45.45454545454545"
                     /fragment="True"
                     /other="intron"
                     /label="small t intron"
     CDS             3568..3705
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="6.497175141242938"
                     /fragment="True"
                     /other="CDS"
                     /label="large T antigen"
     polyA_signal    complement(3728..3862)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.265"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
                     /label="SV40 poly(A) signal"
     CDS             complement(3867..3916)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.62962962962963"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     CDS             3916..3986
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="2.333990795529257"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZ"
     rep_origin      3988..4442
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.781"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     promoter        4468..4524
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="55.88235294117647"
                     /fragment="True"
                     /other="promoter"
                     /label="AmpR promoter"
     promoter        4677..4728
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="72.22222222222221"
                     /fragment="True"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             4729..5589
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     protein_bind    complement(5691..5724)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="97.059"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="loxP"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
      841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      961 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1021 aggccctttc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     1081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agcatcgtgc aggtcgttac
     1141 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     1201 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     1261 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     1321 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     1381 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     1441 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1501 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1561 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1621 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga ggcgccatcc
     1681 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagccta
     1741 ggcttttgca aaaagctatt taggtgacac tatagaaggt acgcctgcag gtaccggtcc
     1801 ggaattcgcc cttatgagag ccctggtagt catccgcctg tcccgcgtca ccgatgctac
     1861 gacttcaccg gagcgtcagc tggagtcttg ccagcagctc tgcgcccagc gcggctggga
     1921 cgtcgtcggg gtagcggagg atctggacgt ctccggggcg gtcgatccgt tcgaccggaa
     1981 gcgcagaccg aacctggccc ggtggctagc gttcgaggag caaccgttcg acgcgatcgt
     2041 ggcgtaccgg gtagaccggt tgacccgatc gatccggcat ctgcagcagc tggtccactg
     2101 ggccgaggac cacaagaagc tggtcgtctc cgcgaccgaa gcgcacttcg atacgacgac
     2161 gccgtttgcg gcggtcgtca tcgcgcttat gggaacggtg gcgcagatgg aattagaagc
     2221 gatcaaagag cggaaccgtt cggctgcgca tttcaatatc cgcgccggga aataccgagg
     2281 atccctgccg ccgtggggat acctgcctac gcgcgtggac ggggagtggc ggctggtgcc
     2341 ggaccctgtg cagcgagagc gcatcctcga ggtgtatcac cgcgtcgtcg acaaccacga
     2401 gccgctgcac ctggtggccc acgacctgaa ccggcgtggt gtcctgtcgc cgaaggacta
     2461 cttcgcgcag ctgcaaggcc gcgagccgca gggccgggag tggtcggcta ccgcgctgaa
     2521 gcgatcgatg atctccgagg cgatgctcgg gtacgcgact ctgaacggta agaccgtccg
     2581 agacgacgac ggagccccgc tggtgcgggc tgagccgatc ctgacccgtg agcagctgga
     2641 ggcgctgcgc gccgagctcg tgaagacctc ccgggcgaag cccgcggtgt ctaccccgtc
     2701 gctgctgctg cgggtgttgt tctgtgcggt gtgcggggag cccgcgtaca agttcgccgg
     2761 gggaggacgt aagcacccgc gctaccgctg ccgctcgatg gggttcccga agcactgcgg
     2821 gaacggcacg gtggcgatgg ccgagtggga cgcgttctgc gaggagcagg tgctggatct
     2881 gctcggggac gcggagcgtc tggagaaagt ctgggtagcc ggctcggact ccgcggtcga
     2941 actcgcggag gtgaacgcgg agctggtgga cctgacgtcg ctgatcggct ccccggccta
     3001 ccgggccggc tctccgcagc gagaagcact ggatgcccgt attgcggcgc tggccgcgcg
     3061 gcaagaggag ctggagggtc tagaggctcg cccgtctggc tgggagtggc gcgagaccgg
     3121 gcagcggttc ggggactggt ggcgggagca ggacaccgcg gcaaagaaca cctggcttcg
     3181 gtcgatgaac gttcggctga cgttcgacgt ccgcggtggg ctgactcgca cgatcgactt
     3241 cggggatctg caggagtacg agcagcatct caggctcggc agcgtggtcg aacggctaca
     3301 caccgggatg tcgtagaagg gcgaattccc gggtcgacga gctcactagc ttgggatctt
     3361 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     3421 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactag ctgcatatgc
     3481 ttgctgcttg agagttttgc ttactgagta tgatttatga aaatattata cacaggagct
     3541 agtgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct
     3601 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     3661 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     3721 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3841 tgtatcttat catgtctgga tcgatcctgc attaatgaat cggccaacgc gcggggagag
     3901 gcggtttgcg tattggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     3961 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4141 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4201 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4261 cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa cactcaaccc
     4321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4441 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4501 cattcaaata tgtatccgct catgccaggt cttggactgg tgagaacggc ttgctcggca
     4561 gcttcgatgt gtgctggagg gagaataaag gtctaagatg tgcgatagag ggaagtcgca
     4621 ttgaattatg tgctgtgtag ggatcgctgg tatcaaatat gtgtgcccac ccctggcatg
     4681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgcc ataacttcgt
     5701 ataatgtatg ctatacgagg ttatggcatg accaaaatcc cttaacgtga gttttcgttc
     5761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
