LOCUS       stock3865_pEW76         4290 bp    DNA     circular UNA 11-JUL-2019
DEFINITION  YFP-miniTurboID-linker in pGGB000.
ACCESSION   urn.local.eva-sophiewallner.6d-an08gz2
VERSION     urn.local.eva-sophiewallner.6d-an08gz2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            201..1061
                     /label="Ampicilin"
     gene            1216..1835
                     /label="pBR322_Origin"
     promoter        2144..2173
                     /label="Lac_promoter"
     primer          2171..2189
                     /label="primer_seq_GG1_for"
     misc_feature    2182..2198
                     /feature_type="Misc."
                     /label="Lac_operator"
     misc_feature    2208..2231
                     /feature_type="Misc."
                     /label="pGEM-T easy [2653-2674]"
     primer          2212..2229
                     /label="SP6_primer"
     misc_feature    2231..2234
                     /feature_type="Misc."
                     /label="Auxiliary bases for SP6"
     misc_feature    2248..3859
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:2c-aka7ctn"
                     /label="YFP-miniTID (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     ligation        2248..2251
                     /label="Ligation"
     misc_feature    <2248..2250
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Primer          <2248..2264
                     /created_by="eva-sophiewallner"
                     /label="1I3"
     misc_feature    2248..2251
                     /feature_type="Misc."
                     /label="02 BC_Oligopaa"
     CDS             2251..2967
                     /note="yellow fluorescent protein"
                     /modified_by="eva-sophiewallner"
                     /label="yellow fluorescent protein"
     misc_feature    2251..2983
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:6j-an08h04"
                     /label="Stock1312 pENTR/D-TOPO YFP (-stop) extraction
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <2251..2254
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:6j-an08h04"
                     /label="Stock1312 pENTR/D-TOPO YFP (-stop) - Source_1
                     (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     source          join(2971..>2983,<2251..2254)
                     /invitrogen=662
                     /label="Source_1"
     source          <2251..>2967
                     /organism="Gateway binary vector R4pGWB440"
                     /mol_type="other DNA"
                     /db_xref="taxon:477453"
                     /label="source Gateway binary vector R4pGWB440"
     misc_feature    2455..2460
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    2678..2683
                     /Recognition_pattern="W^GTACW"
                     /annotation_group="WGTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     misc_feature    2960..2965
                     /Recognition_pattern="W^GTACW"
                     /annotation_group="WGTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     protein_binding complement(2971..2975)
                     /invitrogen=2460000
                     /label="TOPO binding site"
     misc_feature    2978..2985
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, SgsI"
                     /note="Geneious type: restriction site"
     ligation        2980..2983
                     /label="Ligation"
     motif           <2980..2985
                     /created_by="User"
                     /modified_by="User"
                     /label="AscI site"
     misc_feature    2980..>3855
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:6i-an08h04"
                     /label="V5-hBirA-miniTurbo PCR (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     polylinker      2986..3009
                     /created_by="User"
                     /modified_by="User"
                     /label="GS linker"
     misc            3010..3831
                     /created_by="User"
                     /modified_by="User"
                     /label="BirA cassette"
     source          <3010..>3831
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    3013..3054
                     /note="V5 tag"
                     /label="V5 tag"
     misc_feature    3055..3060
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    3055..3060
                     /note="NheI"
                     /label="NheI"
     CDS             3061..3831
                     /codon_start=1
                     /note="BirA (humanized)"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDAC
                     IAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGA
                     DKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="BirA (humanized) CDS"
     misc_feature    3064..3066
                     /note="Q65P"
                     /label="Q65P"
     misc_feature    3094..3099
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    3130..3135
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    3130..3132
                     /note="I87V"
                     /label="I87V"
     misc_feature    complement(3174..3179)
                     /Recognition_pattern="CTCTTC(1/4)"
                     /annotation_group="CTCTTC"
                     /label="EarI"
                     /note="Geneious type: restriction site"
     misc_feature    3214..3216
                     /note="G115G"
                     /label="G115G"
     misc_feature    3223..3225
                     /note="R118S"
                     /label="R118S"
     misc_feature    3289..3291
                     /note="E141K"
                     /label="E141K"
     misc_difference 3292..3294
                     /note="Q142R"
                     /label="Q142R difference"
     misc_feature    3315..3320
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    3316..3318
                     /note="S150G"
                     /label="S150G"
     misc_feature    3319..3321
                     /note="L151P"
                     /label="L151P"
     misc_feature    3346..3348
                     /note="V160A"
                     /label="V160A"
     misc_feature    3403..3408
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    3442..3444
                     /note="T192A"
                     /label="T192A"
     misc_feature    3448..3450
                     /note="K194I"
                     /label="K194I"
     misc_feature    3493..3495
                     /note="M209V"
                     /label="M209V"
     misc_feature    3544..3549
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    3636..3641
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     misc_feature    3701..3706
                     /Recognition_pattern="T^GATCA"
                     /annotation_group="TGATCA"
                     /label="BclI"
                     /note="Geneious type: restriction site"
     misc_structure  3739..3741
                     /note="G291G"
                     /label="G291G misc structure"
     misc_feature    3781..3783
                     /note="I305V"
                     /label="I305V"
     misc_feature    3832..>3859
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     polylinker      3832..3855
                     /created_by="User"
                     /label="GS linker"
     Primer          complement(3841..>3859)
                     /created_by="eva-sophiewallner"
                     /label="1I4"
     ligation        3856..3859
                     /label="Ligation"
     misc_feature    3856..2251
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:1o-akaoi2r"
                     /label="pEW2 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3873..3876
                     /feature_type="Misc."
                     /label="Auxiliary bases for T7"
     misc_feature    3873..3896
                     /feature_type="Misc."
                     /label="pGEM-T easy [2653-2674]"
     promoter        3877..3895
                     /label="T7_promoter"
     CDS             3897..4142
                     /label="Open reading frame1"
     primer          3905..3927
                     /label="M13_pUC_fwd_primer"
     gene            3905..4048
                     /label="LacZ_a"
     primer          complement(3915..3932)
                     /label="primer_seq_GG1_rev"
     primer          4240..4262
                     /label="pGEX_3_primer"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaaac atggtgagca agggcgagga gctgttcacc
     2281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     2461 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2521 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2701 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2821 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     2881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2941 actctcggca tggacgagct gtacaaggct aagggtgggc gcgccggtgg aggcggttca
     3001 ggaggtggca tgggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc
     3061 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     3121 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     3181 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     3241 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     3301 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     3361 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     3421 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     3481 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     3541 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     3601 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     3661 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     3721 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     3781 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gggaggcggt
     3841 ggaagcggcg gaggtggctt gagaccgaat tctcgcccta tagtgagtcg tattacctgg
     3901 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3961 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4021 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     4081 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4141 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4201 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4261 ggttttcacc gtcatcaccg aaacgcgcga
//
