LOCUS       stock3864_pEW75         4308 bp    DNA     circular UNA 11-JUL-2019
DEFINITION  linker-miniTurboID-YFP in pGGD000.
ACCESSION   urn.local.eva-sophiewallner.4x-an08cnw
VERSION     urn.local.eva-sophiewallner.4x-an08cnw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     gene            201..1061
                     /label="Ampicilin"
     Region          1216..1835
                     /label="pBR322_Origin"
     promoter        2144..2173
                     /label="Lac_promoter"
     primer          2171..2189
                     /label="primer_seq_GG1_for"
     misc_feature    2182..2198
                     /feature_type="Misc."
                     /label="Lac_operon"
     misc_feature    2208..2231
                     /feature_type="Misc."
                     /label="pGEM-T easy [2653-2674]"
     primer          2212..2229
                     /label="SP6_primer"
     misc_feature    2231..2234
                     /feature_type="Misc."
                     /label="Auxiliary bases for SP6"
     misc_feature    2248..3877
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:2h-aka7iwl"
                     /label="BirAminiTID-YFP (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     Primer          <2248..2301
                     /created_by="eva-sophiewallner"
                     /label="1I1"
     misc_feature    <2248..2249
                     /Original_Bases="TCCCGCTGCTGAACGCTAAACAGATTCTGGGACAGCTGGAC
                     GGCGGGAGCGTGGCAGTCCTGCCTGTGGTCGACTCCACCAATCAGTACCTGCTGGATC
                     GAATCGGCGAGCTGAAGAGTGGGGATGCTTGCATTGCAGAATATCAGCAGGCAGGGAG
                     AGGAAGCAGAGGGAGGAAATGGTTCTCTCCTTTTGGAGCTAACCTGTACCTGAGTATG
                     TTTTGGCGCCTGAAGCGGGGACCAGCAGCAATCGGCCTGGGCCCGGTCATCGGAATTG
                     TCATGGCAGAAGCGCTGCGAAAGCTGGGAGCAGACAAGGTGCGAGTCAAATGGCCCAA
                     TGACCTGTATCTGCAGGATAGAAAGCTGGCAGGCATCCTGGTGGAGCTGGCCGGAATA
                     ACAGGCGATGCTGCACAGATCGTCATTGGCGCCGGGATTAACGTGGCTATGAGGCGCG
                     TGGAGGAAAGCGTGGTCAATCAGGGCTGGATCACACTGCAGGAAGCAGGGATTAACCT
                     GGACAGGAATACTCTGGCCGCTATGCTGATCCGAGAGCTGCGGGCAGCCCTGGAACTG
                     TTCGAGCAGGAAGGCCTGGCTCCATATCTGTCACGGTGGGAGAAGCTGGATAACTTCA
                     TCAATAGACCCGTGAAGCTGATCATTGGGGACAAAGAGATTTTCGGGATTAGCCGGGG
                     GATTGATAAACAGGGAGCCCTGCTGCTGGAACAGGACGGAGTTATCAAACCCTGGATG
                     GGCGGAGAAATCAGTCTGCGGTCTGCCGAAAAGGGAGGCGGTGGAAGCGGCGGAGGTT
                     CCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCT
                     GGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCC
                     ACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCT
                     GGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGA
                     CCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
                     CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCG
                     AGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGG
                     CAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATG
                     GCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGG
                     ACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCC
                     CGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCC
                     AACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC
                     TCGGCATGGACGAGCTGTACAAGT"
                     /label="TCCCGCTGCTGAACGCTAAACAGATTCTGGGACAGCTGGACGGCGGGAGC
                     GTGGCAGTCCTGCCTGTGGTCGACTCCACCAATCAGTACCTGCTGGATCGAATCGGCG
                     AGCTGAAGAGTGGGGATGCTTGCATTGCAGAATATCAGCAGGCAGGGAGAGGAAGCAG
                     AGGGAGGAAATGGTTCTCTCCTTTTGGAGCTAACCTGTACCTGAGTATGTTTTGGCGC
                     CTGAAGCGGGGACCAGCAGCAATCGGCCTGGGCCCGGTCATCGGAATTGTCATGGCAG
                     AAGCGCTGCGAAAGCTGGGAGCAGACAAGGTGCGAGTCAAATGGCCCAATGACCTGTA
                     TCTGCAGGATAGAAAGCTGGCAGGCATCCTGGTGGAGCTGGCCGGAATAACAGGCGAT
                     GCTGCACAGATCGTCATTGGCGCCGGGATTAACGTGGCTATGAGGCGCGTGGAGGAAA
                     GCGTGGTCAATCAGGGCTGGATCACACTGCAGGAAGCAGGGATTAACCTGGACAGGAA
                     TACTCTGGCCGCTATGCTGATCCGAGAGCTGCGGGCAGCCCTGGAACTGTTCGAGCAG
                     GAAGGCCTGGCTCCATATCTGTCACGGTGGGAGAAGCTGGATAACTTCATCAATAGAC
                     CCGTGAAGCTGATCATTGGGGACAAAGAGATTTTCGGGATTAGCCGGGGGATTGATAA
                     ACAGGGAGCCCTGCTGCTGGAACAGGACGGAGTTATCAAACCCTGGATGGGCGGAGAA
                     ATCAGTCTGCGGTCTGCCGAAAAGGGAGGCGGTGGAAGCGGCGGAGGTTCCATGGTGA
                     GCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGA
                     CGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGC
                     AAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCC
                     TCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAA
                     GCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATC
                     TTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACA
                     CCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCT
                     GGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAG
                     CAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG
                     TGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCT
                     GCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAG
                     CGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGG
                     ACGAGCTGTACAAGT"
                     /note="Geneious type: Editing History Replacement"
     ligation        2248..2251
                     /label="Ligation"
     misc_feature    2248..2251
                     /label="02 DE_Oligopaar"
     polylinker      2251..2274
                     /created_by="User"
                     /modified_by="User"
                     /label="GS linker"
     misc_feature    2252..>3877
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc            2275..3096
                     /created_by="User"
                     /modified_by="User"
                     /label="BirA cassette"
     source          <2275..>3096
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    2278..2319
                     /note="V5 tag"
                     /label="V5 tag"
     misc_feature    2320..2325
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI"
                     /note="Geneious type: restriction site"
     misc_feature    2320..2325
                     /note="NheI"
                     /label="NheI"
     CDS             2326..3096
                     /codon_start=1
                     /note="BirA (humanized)"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDAC
                     IAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGA
                     DKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="BirA (humanized) CDS"
     misc_feature    2329..2331
                     /note="Q65P"
                     /label="Q65P"
     misc_feature    2359..2364
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    2395..2400
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    2395..2397
                     /note="I87V"
                     /label="I87V"
     misc_feature    2479..2481
                     /note="G115G"
                     /label="G115G"
     misc_feature    2488..2490
                     /note="R118S"
                     /label="R118S"
     misc_feature    2554..2556
                     /note="E141K"
                     /label="E141K"
     misc_difference 2557..2559
                     /note="Q142R"
                     /label="Q142R difference"
     primer_bind     2560..2576
                     /Mismatches=0
                     /%GC="64.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /annotation_group="1111288746569031f37d647a-59c1-4680-8b62
                     -5276eca0c283"
                     /Sequence="GGACCAGCAGCAATCGG"
                     /created_by="primer3"
                     /label="oAM0041 BirA-seq1 "
     primer_bind     2560..2576
                     /Mismatches=0
                     /%GC="64.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.1"
                     /annotation_group="5024016097486521f839e9a-2240-4ff8-83a9-
                     2e9a0d7a39cb"
                     /Sequence="GGACCAGCAGCAATCGG"
                     /created_by="primer3"
                     /label="oAM0041 BirA-seq1 "
     misc_feature    2580..2585
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2581..2583
                     /note="S150G"
                     /label="S150G"
     misc_feature    2584..2586
                     /note="L151P"
                     /label="L151P"
     misc_feature    2611..2613
                     /note="V160A"
                     /label="V160A"
     misc_feature    2668..2673
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    2707..2709
                     /note="T192A"
                     /label="T192A"
     misc_feature    2713..2715
                     /note="K194I"
                     /label="K194I"
     misc_feature    complement(2758..2773)
                     /Mismatches=0
                     /%GC="68.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.8"
                     /annotation_group="11112888084271977e55a7d2-4523-4b2d-a6bf
                     -df85ae26b82e"
                     /Sequence="CGCGCCTCATAGCCAC"
                     /created_by="primer3"
                     /label="oAM0042 BirA-seq2 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2758..2773)
                     /Mismatches=0
                     /%GC="68.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.8"
                     /annotation_group="50240164745929113b1d5dd-9d78-48fc-91b1-
                     64a76f412d79"
                     /Sequence="CGCGCCTCATAGCCAC"
                     /created_by="primer3"
                     /label="oAM0042 BirA-seq2 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2758..2760
                     /note="M209V"
                     /label="M209V"
     misc_feature    2809..2814
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    2901..2906
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI"
                     /note="Geneious type: restriction site"
     misc_feature    2966..2971
                     /Recognition_pattern="T^GATCA"
                     /annotation_group="TGATCA"
                     /label="BclI"
                     /note="Geneious type: restriction site"
     misc_structure  3004..3006
                     /note="G291G"
                     /label="G291G misc structure"
     misc_feature    3046..3048
                     /note="I305V"
                     /label="I305V"
     polylinker      3097..3120
                     /created_by="User"
                     /label="GS linker"
     misc_feature    3122..3127
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    <3123..3873
                     /label="Insert"
                     /note="Geneious type: Inserted sequence"
     source          3123
                     /invitrogen=662
                     /label="Source_1"
     ORF             3124..3840
                     /created_by="User"
                     /label="YFP"
     misc_feature    complement(3169..3190)
                     /Mismatches=0
                     /%GC="72.7"
                     /Hairpin_Tm="45.8"
                     /Self_Dimer_Tm="15.2"
                     /Tm="69.6"
                     /annotation_group="1111288710429445b6c05abe-2e44-4617-bbba
                     -9d8d33bdd743"
                     /Sequence="CGTCGCCGTCCAGCTCGACCAG"
                     /created_by="primer3"
                     /label="EGFP-N"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3169..3190)
                     /Mismatches=0
                     /%GC="72.7"
                     /Hairpin_Tm="45.8"
                     /Self_Dimer_Tm="15.2"
                     /Tm="69.6"
                     /annotation_group="50240157379113053b0ad79-5a70-4392-aced-
                     3d08f2ea45b9"
                     /Sequence="CGTCGCCGTCCAGCTCGACCAG"
                     /created_by="primer3"
                     /label="EGFP-N"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3328..3333
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3417..3438)
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="61.6"
                     /annotation_group="1111288868447481e82df8f4-52a0-40ae-b4fe
                     -3be5490c20c1"
                     /Sequence="GCCGTCGTCCTTGAAGAAGATG"
                     /created_by="primer3"
                     /label="oAM0054 YFP R2 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3417..3438)
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="61.6"
                     /annotation_group="11112889031076386b51c2bb-2017-40b1-b09b
                     -48b27d3da751"
                     /Sequence="GCCGTCGTCCTTGAAGAAGATG"
                     /created_by="primer3"
                     /label="YFP R2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3417..3438)
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="61.6"
                     /annotation_group="502401708172938243a2e82-15ce-4b52-ae39-
                     33181cea0d8a"
                     /Sequence="GCCGTCGTCCTTGAAGAAGATG"
                     /created_by="primer3"
                     /label="oAM0054 YFP R2 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3417..3438)
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="32.9"
                     /Self_Dimer_Tm="None"
                     /Tm="61.6"
                     /annotation_group="502401768977813db4f7d30-0dd4-48c4-a39f-
                     bc84ef8ca530"
                     /Sequence="GCCGTCGTCCTTGAAGAAGATG"
                     /created_by="primer3"
                     /label="YFP R2"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3777..3798
                     /Mismatches=0
                     /%GC="59.1"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="63.4"
                     /annotation_group="1111288650538022ad62c90a-6f82-4144-9c28
                     -2c30e3754023"
                     /Sequence="CATGGTCCTGCTGGAGTTCGTG"
                     /created_by="primer3"
                     /label="EGFP-C"
     primer_bind     3777..3798
                     /Mismatches=0
                     /%GC="59.1"
                     /Hairpin_Tm="44.2"
                     /Self_Dimer_Tm="13.4"
                     /Tm="63.4"
                     /annotation_group="502401512664292d8f7626e-e71b-4cf6-a524-
                     4b8fcf9a5cb5"
                     /Sequence="CATGGTCCTGCTGGAGTTCGTG"
                     /created_by="primer3"
                     /label="EGFP-C"
     misc_feature    3846..3851
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     Primer          complement(3847..>3877)
                     /created_by="eva-sophiewallner"
                     /label="1I2"
     ORF             3850..3870
                     /created_by="User"
                     /label="classical NLS"
     ligation        3874..3877
                     /label="Ligation"
     misc_feature    3874..2251
                     /Source="referenceToDocument:
                     urn:local:eva-sophiewallner:8c-akamamt"
                     /label="pEW4 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3891..3914
                     /feature_type="Misc."
                     /label="pGEM-T easy [2653-2674]"
     misc_feature    3891..3894
                     /feature_type="Misc."
                     /label="Auxiliary bases for T7"
     promoter        3895..3913
                     /label="T7_promoter"
     gene            3915..4160
                     /label="Open reading frame1"
     primer          3923..3945
                     /label="M13_pUC_fwd_primer"
     gene            3923..4066
                     /label="LacZ_a"
     primer          complement(3933..3950)
                     /label="primer_seq_GG1_rev"
     primer          4258..4280
                     /label="pGEX_3_primer"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcatca ggtggaggcg gttcaggagg tggcatgggc
     2281 aagcccatcc ccaaccccct gctgggcctg gacagcaccg ctagcatccc gctgctgaac
     2341 gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac
     2401 tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt
     2461 gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga
     2521 gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg
     2581 ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg
     2641 cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg
     2701 gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg
     2761 gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca
     2821 gggattaacc tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc
     2881 ctggaactgt tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat
     2941 aacttcatca atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc
     3001 cgggggattg ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg
     3061 atgggcggag aaatcagtct gcggtctgcc gaaaagggag gcggtggaag cggcggaggt
     3121 tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3301 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     3361 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3721 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3841 gcttcggatc caaaaaagaa gagaaaggta tagctgctga gaccgaattc tcgccctata
     3901 gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3961 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4021 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4081 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     4141 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4201 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4261 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
