LOCUS       pCHW00014_VP16-NLS-pUC5 2945 bp ds-DNA     linear       30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(239..307)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     378..395
                     /label="M13-fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    443..700
                     /label="VP16-NLS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             443..700
                     /label="Translation 443-700"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGGPKKKRKV*"
     primer_bind     complement(765..785)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(791..813)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(817..847)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(1153..1835)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tGGTCTCATT CGGCCCCCCC GACCGATGTC AGCCTGGGGG ACGAGCTCCA
      481 CTTAGACGGC GAGGACGTGG CGATGGCGCA TGCCGACGCG CTAGACGATT TCGATCTGGA
      541 CATGTTGGGG GACGGGGATT CCCCGGGTCC GGGATTTACC CCCCACGACT CCGCCCCCTA
      601 CGGCGCTCTG GATATGGCCG ACTTCGAGTT TGAGCAGATG TTTACCGATG CCCTTGGAAT
      661 TGACGAGTAC GGTGGGCCTA AAAAGAAGCG TAAAGTCTAA GCTTAGAGAC Catcggatcc
      721 cgggcccgtc gactgcagag gcctgcatgc aagcttggct cgagcatggt catagctgtt
      781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      841 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      901 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1861 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1921 gtctgacagt tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     1981 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     2041 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     2101 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     2161 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     2221 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     2281 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     2341 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     2401 atcaggatat tcttctaata cctggaatgc tgttttccca gggatcgcag tggtgagtaa
     2461 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     2521 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     2581 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     2641 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     2701 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca
     2761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2881 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2941 tcgtc
//