LOCUS       Exported                7033 bp ds-DNA     circular SYN 10-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   ????
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    524..1597
                     /label=THR1
     rep_origin      complement(1753..2208)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2600..2623)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        2670..3334
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             3381..3410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      complement(3872..4460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4631..5491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5623..6965
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcac cctgcaggct taaaattatc ggttctagcg
      241 atgttagagt acaacctctg aacgccgttc cttagcatct tttgttatta atcagctctc
      301 tgctttgcat taatttgacc acctgccctg aaacaccttt cttctagtcc acctgtgtag
      361 gctttttcac ctgaattgtt cattctcgat acgccctcac gccggatgaa aaatttgtgc
      421 aagagtcatc atctcgaaaa gatatggtga taaaaacaag tgtacttcta acctgcctaa
      481 tggttataac agtagcataa agtagatata cggactacag aaaatggttc gtgccttcaa
      541 aattaaagtt ccagcttctt ccgccaatat cggccctggt tatgatgttc ttggtgtcgg
      601 tctttctcta ttcttggagt tagatgtcac catcgactcc agccaagctc aggaaacaaa
      661 cgacgatccc aacaactgca agctgtctta cactaaagaa agtgaaggct attctacggt
      721 cccattgcgt tctgatgcta acctgattac caggactgcg ttatatgtgt tgcgttgcaa
      781 caatattaga aacttcccct ccggaaccaa agttcacgtc tccaacccaa tcccacttgg
      841 ccgtggattg ggttcctctg gtgcagcagt tgtggcaggt gttattttag gtaacgaagt
      901 ggcccaattg ggtttctcta agcaacgtat gttggactac tgtttgatga ttgaacgtca
      961 tccagacaac ataaccgctg ctatgatggg aggcttttgc ggttcattcc taagggactt
     1021 gaccccacaa gaggtggaaa gacgtgagat tccattggct gaggtgcttc cagaaccttc
     1081 tggtggtgaa gataccggtc tggttccccc attacctccc accgatatcg gtagacatgt
     1141 caaataccaa tggaaccccg ccattaaatg tattgcgatc atcccacagt tcgagttgtc
     1201 caccgccgac tccagaggcg ttcttccaaa agcctaccca acccaggact tggttttcaa
     1261 tctacaaaga ttggccgtct tgaccacagc tttgaccatg gacccaccta atgccgactt
     1321 aatctaccct gctatgcaag atcgtgtcca ccaaccttat agaaagacat tgatcccagg
     1381 tctcacggaa atcttatcat gtgtcacccc atccacatac cctggcctat tgggtatctg
     1441 cttgtcaggt gcaggcccaa ctatcttggc tttggccact gagaatttcg aagaaatctc
     1501 tcaagaaatt atcaacaggt tcgccaaaaa cggcatcaag tgctcctgga aactactgga
     1561 gcctgcctac gatggtgcaa gcgtcgaaca gcaatgaaat gtcctttacc cctagtatac
     1621 tgtgttaaat actattgtag accatataca tactgtaatt gaccgttaac ggttattatg
     1681 ctgagtcttc ttccgttacc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1741 taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     1801 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     1861 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     1921 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     1981 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2041 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     2101 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     2161 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     2221 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2281 gaattggagc gacctcatgc tatacctgag aaagcaacct gacctacagg aaagagttac
     2341 tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg tatacactta
     2401 ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc gcttatttag
     2461 aagtgtcaac aacgtatcta ccaacgattt gacccttttc catcttttcg taaatttctg
     2521 gcaaggtaga caagccgaca accttgattg gagacttgac caaacctctg gcgaagaatt
     2581 gttaattaag agctcagatc ttatcgtcgt catccttgta atccatcgat actagtgcgg
     2641 ccgcccttta gtgagggttg aattcgaatt ttcaaaaatt cttacttttt ttttggatgg
     2701 acgcaaagaa gtttaataat catattacat ggcattacca ccatatacat atccatatac
     2761 atatccatat ctaatcttac ttatatgttg tggaaatgta aagagcccca ttatcttagc
     2821 ctaaaaaaac cttctctttg gaactttcag taatacgctt aactgctcat tgctatattg
     2881 aagtacggat tagaagccgc cgagcgggtg acagccctcc gaaggaagac tctcctccgt
     2941 gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct
     3001 ccgaacaata aagattctac aatactagct tttatggtta tgaagaggaa aaattggcag
     3061 taacctggcc ccacaaacct tcaaatgaac gaatcaaatt aacaaccata ggatgataat
     3121 gcgattagtt ttttagcctt atttctgggg taattaatca gcgaagcgat gatttttgat
     3181 ctattaacag atatataaat gcaaaaactg cataaccact ttaactaata ctttcaacat
     3241 tttcggtttg tattacttct tattcaaatg taataaaagt atcaacaaaa aattgttaat
     3301 atacctctat actttaacgt caaggagaaa aaaccccgga tccgtaatac gactcactat
     3361 agggcccggg cgtcgacatg gaacagaagt tgatttccga agaagacctc gagtaagctt
     3421 ggtaccgcgg ctagctaaga tccgctctaa ccgaaaagga aggagttaga caacctgaag
     3481 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     3541 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     3601 tgcttgagaa ggttttggga cgctcgaaga tccagctgca ttaatgaatc ggccaacgcg
     3661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5581 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
     5641 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     5701 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
     5761 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     5821 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
     5881 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
     5941 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
     6001 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
     6061 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
     6121 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
     6181 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
     6241 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
     6301 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
     6361 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
     6421 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
     6481 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
     6541 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     6601 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     6661 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     6721 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     6781 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     6841 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     6901 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     6961 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7021 aggccctttc gtc
//
