LOCUS       Exported                7294 bp ds-DNA     circular SYN 20-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    614..1876
                     /label=ARG1
     rep_origin      complement(2014..2469)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2861..2884)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        2931..3595
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     CDS             3642..3671
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      complement(4133..4721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4892..5752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5884..7226
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcaa taagagcgct atgttagtca ctactgacgg
      241 ctctccagtc atttatgtga ttttttagtg actcatgtcg catttggccc gtttttttcc
      301 gctgtcgcaa cctatttcca ttaacggtgc cgtatggaag agtcatttaa aggcaggaga
      361 gagagattac tcatcttcat tggatcagat tgatgactgc gtagcggcag atagtgtaat
      421 ctgagcagtt gcgagaccca gactggcact gtctcaatag tatattaatg ggcatacatt
      481 cgtactccct tgttcttgcc cacagttctc tctctcttta cttcttgtat cttgtctccc
      541 cattgtgcag cgataaggaa cattgttcta atatacacgg atacaaaaga aatacacata
      601 attgcataaa ataatgtcta agggaaaagt ttgtttggct tattctggtg gtttagatac
      661 ctccgtcatt ttggcttggc tactagacca aggctacgaa gttgtagctt tcatggctaa
      721 tgtagggcaa gaagaagatt tcgatgccgc caaggaaaag gccttgaaga tcggtgcctg
      781 caagttcgtt tgtgtggatt gtcgtgaaga ttttgtcaag gatattctat tcccagctgt
      841 acaggtcaac gctgtgtacg aagacgttta tctgttgggt acttctttgg caagacctgt
      901 tattgccaaa gcccaaattg acgtcgctaa acaggagggc tgtttcgcgg tctctcatgg
      961 ttgtaccggt aaaggtaatg atcaaatcag attcgaattg tcattttacg ctctgaagcc
     1021 agacgttaag tgtattacac catggagaat gcctgaattt ttcgaaagat ttgctggcag
     1081 aaaggatttg ttagactatg ctgcacaaaa gggtattccc gtcgcccaaa ccaaggccaa
     1141 gccatggtct actgacgaaa accaagccca catttcttac gaggcaggta tcttggaaga
     1201 cccagatacc accccaccaa aggacatgtg gaaattgatc gtcgatccaa tggatgctcc
     1261 ggaccaacca caagatttga ccattgactt tgaacgtggt cttccagtca agttgaccta
     1321 caccgacaac aagacttcca aggaagtttc cgttaccaag cctttggatg ttttcttggc
     1381 cgcatccaac ttagcaaggg ccaacggtgt tggtagaatt gatattgtag aagatcgtta
     1441 cattaacttg aaatccagag gttgttacga acaggctcca ttgactgttt tgagaaaagc
     1501 tcatgttgat ttggaaggtt tgactttaga caaagaagtc cgtcaattga gagactcatt
     1561 cgtcacacca aactactcca gattgatata taacggttcc tacttcaccc cagagtgtga
     1621 gtacatcaga tctatgatcc aaccatccca aaatagcgtt aacggtactg tcagggttag
     1681 actgtataag ggtaacgtca tcattctggg cagatctaca aagactgaaa agttgtacga
     1741 tccgacagaa tcctctatgg atgagttgac cggtttctta cctaccgata ccaccggttt
     1801 cattgccatc caggccatta gaattaaaaa atacggtgaa tccaaaaaaa ccaaaggtga
     1861 agagttgact ttgtaagtcc gctagttcat cgcctcaaga tagataacga tctcttcctc
     1921 cacctcctat ttctgcacac tcttgtgatg taacccggga atatgcggtg tgaaataccg
     1981 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa
     2041 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     2101 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     2161 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     2221 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2281 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2341 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     2401 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     2461 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     2521 ctcttcgcta ttacgccagc tgaattggag cgacctcatg ctatacctga gaaagcaacc
     2581 tgacctacag gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac
     2641 tttaaaattt gtatacactt atttttttta taacttattt aataataaaa atcataaatc
     2701 ataagaaatt cgcttattta gaagtgtcaa caacgtatct accaacgatt tgaccctttt
     2761 ccatcttttc gtaaatttct ggcaaggtag acaagccgac aaccttgatt ggagacttga
     2821 ccaaacctct ggcgaagaat tgttaattaa gagctcagat cttatcgtcg tcatccttgt
     2881 aatccatcga tactagtgcg gccgcccttt agtgagggtt gaattcgaat tttcaaaaat
     2941 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc
     3001 accatataca tatccatata catatccata tctaatctta cttatatgtt gtggaaatgt
     3061 aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct
     3121 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc
     3181 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
     3241 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
     3301 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
     3361 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc
     3421 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac
     3481 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag
     3541 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaaccccgg
     3601 atccgtaata cgactcacta tagggcccgg gcgtcgacat ggaacagaag ttgatttccg
     3661 aagaagacct cgagtaagct tggtaccgcg gctagctaag atccgctcta accgaaaagg
     3721 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     3781 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     3841 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag atccagctgc
     3901 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4561 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5881 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     5941 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     6001 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     6061 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     6121 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     6181 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     6241 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     6301 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     6361 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     6421 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     6481 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     6541 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     6601 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     6661 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     6721 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
     6781 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
     6841 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
     6901 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
     6961 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
     7021 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
     7081 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
     7141 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
     7201 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
     7261 tataaaaata ggcgtatcac gaggcccttt cgtc
//
