LOCUS       Exported               10349 bp ds-DNA     circular SYN 09-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   ????
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             459..4637
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     rep_origin      complement(5069..5524)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5986..6650
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     rep_origin      complement(7188..7776)
                     /direction=LEFT
                     /label=pUC ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7947..8807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8939..10281
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat gccagcggaa ttccacttgc aattacataa
      241 aaaattccgg cggtttttcg cgtgtgactc aatgtcgaaa tacctgccta atgaacatga
      301 acatcgccca aatgtatttg aagacccgct gggagaagtt caagatatat aagtaacaag
      361 cagccaatag tataaaaaaa aatctgagtt tattaccttt cctggaattt cagtgaaaaa
      421 ctgctaatta tagagagata tcacagagtt actcactaat gactaacgaa aaggtctgga
      481 tagagaagtt ggataatcca actctttcag tgttaccaca tgacttttta cgcccacaac
      541 aagaacctta tacgaaacaa gctacatatt cgttacagct acctcagctc gatgtgcctc
      601 atgatagttt ttctaacaaa tacgctgtcg ctttgagtgt atgggctgca ttgatatata
      661 gagtaaccgg tgacgatgat attgttcttt atattgcgaa taacaaaatc ttaagattca
      721 atattcaacc aacgtggtca tttaatgagc tgtattctac aattaacaat gagttgaaca
      781 agctcaattc tattgaggcc aatttttcct ttgacgagct agctgaaaaa attcaaagtt
      841 gccaagacct ggaaaggacc cctcagttgt tccgtttggc ctttttggaa aaccaagatt
      901 tcaaattaga cgagttcaag catcatttag tggactttgc tttgaatttg gataccagta
      961 ataatgcgca tgttttgaac ttaatttata acagcttact gtattcgaat gaaagagtaa
     1021 ccattgttgc ggaccaattt actcaatatt tgactgctgc gctaagcgat ccatccaatt
     1081 gcataactaa aatctctctg atcaccgcat catccaagga tagtttacct gatccaacta
     1141 agaacttggg ctggtgcgat ttcgtggggt gtattcacga cattttccag gacaatgctg
     1201 aagccttccc agagagaacc tgtgttgtgg agactccaac actaaattcc gacaagtccc
     1261 gttctttcac ttatcgcgac atcaaccgca cttctaacat agttgcccat tatttgatta
     1321 aaacaggtat caaaagaggt gatgtagtga tgatctattc ttctaggggt gtggatttga
     1381 tggtatgtgt gatgggtgtc ttgaaagccg gcgcaacctt ttcagttatc gaccctgcat
     1441 atcccccagc cagacaaacc atttacttag gtgttgctaa accacgtggg ttgattgtta
     1501 ttagagctgc tggacaattg gatcaactag tagaagatta catcaatgat gaattggaga
     1561 ttgtttcaag aatcaattcc atcgctattc aagaaaatgg taccattgaa ggtggcaaat
     1621 tggacaatgg cgaggatgtt ttggctccat atgatcacta caaagacacc agaacaggtg
     1681 ttgtagttgg accagattcc aacccaaccc tatctttcac atctggttcc gaaggtattc
     1741 ctaagggtgt tcttggtaga catttttcct tggcttatta tttcaattgg atgtccaaaa
     1801 ggttcaactt aacagaaaat gataaattca caatgctgag cggtattgca catgatccaa
     1861 ttcaaagaga tatgtttaca ccattatttt taggtgccca attgtatgtc cctactcaag
     1921 atgatattgg tacaccgggc cgtttagcgg aatggatgag taagtatggt tgcacagtta
     1981 cccatttaac acctgccatg ggtcaattac ttactgccca agctactaca ccattcccta
     2041 agttacatca tgcgttcttt gtgggtgaca ttttaacaaa acgtgattgt ctgaggttac
     2101 aaaccttggc agaaaattgc cgtattgtta atatgtacgg taccactgaa acacagcgtg
     2161 cagtttctta tttcgaagtt aaatcaaaaa atgacgatcc aaactttttg aaaaaattga
     2221 aagatgtcat gcctgctggt aaaggtatgt tgaacgttca gctactagtt gttaacagga
     2281 acgatcgtac tcaaatatgt ggtattggcg aaataggtga gatttatgtt cgtgcaggtg
     2341 gtttggccga aggttataga ggattaccag aattgaataa agaaaaattt gtgaacaact
     2401 ggtttgttga aaaagatcac tggaattatt tggataagga taatggtgaa ccttggagac
     2461 aattctggtt aggtccaaga gatagattgt acagaacggg tgatttaggt cgttatctac
     2521 caaacggtga ctgtgaatgt tgcggtaggg ctgatgatca agttaaaatt cgtgggttca
     2581 gaatcgaatt aggagaaata gatacgcaca tttcccaaca tccattggta agagaaaaca
     2641 ttactttagt tcgcaaaaat gccgacaatg agccaacatt gatcacattt atggtcccaa
     2701 gatttgacaa gccagatgac ttgtctaagt tccaaagtga tgttccaaag gaggttgaaa
     2761 ctgaccctat agttaagggc ttaatcggtt accatctttt atccaaggac atcaggactt
     2821 tcttaaagaa aagattggct agctatgcta tgccttcctt gattgtggtt atggataaac
     2881 taccattgaa tccaaatggt aaagttgata agcctaaact tcaattccca actcccaagc
     2941 aattaaattt ggtagctgaa aatacagttt ctgaaactga cgactctcag tttaccaatg
     3001 ttgagcgcga ggttagagac ttatggttaa gtatattacc taccaagcca gcatctgtat
     3061 caccagatga ttcgtttttc gatttaggtg gtcattctat cttggctacc aaaatgattt
     3121 ttaccttaaa gaaaaagctg caagttgatt taccattggg cacaattttc aagtatccaa
     3181 cgataaaggc ctttgccgcg gaaattgaca gaattaaatc atcgggtgga tcatctcaag
     3241 gtgaggtcgt cgaaaatgtc actgcaaatt atgcggaaga cgccaagaaa ttggttgaga
     3301 cgctaccaag ttcgtacccc tctcgtgaat attttgttga acctaatagt gccgaaggaa
     3361 aaacaacaat taatgtgttt gttaccggtg tcacaggatt tctgggctcc tacatccttg
     3421 cagatttgtt aggacgttct ccaaagaact acagtttcaa agtgtttgcc cacgtcaggg
     3481 ccaaggatga agaagctgca tttgcaagat tacaaaaggc aggtatcacc tatggtactt
     3541 ggaacgaaaa atttgcctca aatattaaag ttgtattagg cgatttatct aaaagccaat
     3601 ttggtctttc agatgagaag tggatggatt tggcaaacac agttgatata attatccata
     3661 atggtgcgtt agttcactgg gtttatccat atgccaaatt gagggaccca aatgttattt
     3721 caactatcaa tgttatgagc ttagccgccg tcggcaagcc aaagttcttt gactttgttt
     3781 cctccacttc tactcttgac actgaatact actttaattt gtcagataaa cttgttagcg
     3841 aagggaagcc aggcatttta gaatcagacg atttaatgaa ctctgcaagc gggctcactg
     3901 gtggatatgg tcagtccaaa tgggctgctg agtacatcat tagacgtgca ggtgaaaggg
     3961 gcctacgtgg gtgtattgtc agaccaggtt acgtaacagg tgcctctgcc aatggttctt
     4021 caaacacaga tgatttctta ttgagatttt tgaaaggttc agtccaatta ggtaagattc
     4081 cagatatcga aaattccgtg aatatggttc cagtagatca tgttgctcgt gttgttgttg
     4141 ctacgtcttt gaatcctccc aaagaaaatg aattggccgt tgctcaagta acgggtcacc
     4201 caagaatatt attcaaagac tacttgtata ctttacacga ttatggttac gatgtcgaaa
     4261 tcgaaagcta ttctaaatgg aagaaatcat tggaggcgtc tgttattgac aggaatgaag
     4321 aaaatgcgtt gtatcctttg ctacacatgg tcttagacaa cttacctgaa agtaccaaag
     4381 ctccggaact agacgatagg aacgccgtgg catctttaaa gaaagacacc gcatggacag
     4441 gtgttgattg gtctaatgga ataggtgtta ctccagaaga ggttggtata tatattgcat
     4501 ttttaaacaa ggttggattt ttacctccac caactcataa tgacaaactt ccactgccaa
     4561 gtatagaact aactcaagcg caaataagtc tagttgcttc aggtgctggt gctcgtggaa
     4621 gctccgcagc agcttaaggt tgagcattac gtatgatatg tccatgtaca ataattaaat
     4681 atgaattagg agaaagactt agcttctttt cgggtgatgt cacttaaaaa ctccgagaat
     4741 aatatataat aagagaataa aatattagtt attgaataag aactgtaaat cagctggcgt
     4801 tagtctgcta atggcagctt catcttggtt tattgtagca tgaatcatat ttgccttttt
     4861 ttcctgtaat tcaatgattc ttgcttctat actatcctca atgcaaaacc ttgtgatctt
     4921 cacaggtcga tactgaccaa ttctatgaac tctatcacca ctttgccatt caacactagg
     4981 gttccaccat gggtctaaaa tgaatactcc cgggcctatg cggtgtgaaa taccgcacag
     5041 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
     5101 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5161 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5221 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5281 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5341 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5401 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5461 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5521 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5581 cgctattacg ccagctgaat tggagcgacc tcatgctata cctgagaaag caacctgacc
     5641 tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa
     5701 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
     5761 aaattcgctt atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc
     5821 ttttcgtaaa tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa
     5881 cctctggcga agaattgtta attaagagct cagatcttat cgtcgtcatc cttgtaatcc
     5941 atcgatacta gtgcggccgc cctttagtga gggttgaatt cgaattttca aaaattctta
     6001 cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca ttaccaccat
     6061 atacatatcc atatacatat ccatatctaa tcttacttat atgttgtgga aatgtaaaga
     6121 gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat acgcttaact
     6181 gctcattgct atattgaagt acggattaga agccgccgag cgggtgacag ccctccgaag
     6241 gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc
     6301 ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa
     6361 gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atgaacgaat caaattaaca
     6421 accataggat gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga
     6481 agcgatgatt tttgatctat taacagatat ataaatgcaa aaactgcata accactttaa
     6541 ctaatacttt caacattttc ggtttgtatt acttcttatt caaatgtaat aaaagtatca
     6601 acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac cccggatccg
     6661 taatacgact cactataggg cccgggcgtc gacatggaac agaagttgat ttccgaagaa
     6721 gacctcgagt aagcttggta ccgcggctag ctaagatccg ctctaaccga aaaggaagga
     6781 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
     6841 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
     6901 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaagatcca gctgcattaa
     6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7621 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     8941 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
     9001 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
     9061 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
     9121 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     9181 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     9241 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     9301 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     9361 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     9421 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     9481 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     9541 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     9601 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     9661 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     9721 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     9781 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg
     9841 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
     9901 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     9961 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
    10021 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
    10081 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
    10141 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
    10201 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
    10261 catttccttt gatattggat catactaaga aaccattatt atcatgacat taacctataa
    10321 aaataggcgt atcacgaggc cctttcgtc
//
