LOCUS       Exported               10863 bp ds-DNA     circular SYN 13-JUN-2024
DEFINITION  PHAGE_N__EGFP_ATG9A_FL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1036..1066
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3601..3904
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3905..4108
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4188..4197
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4194..4910
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    4926..4950
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4953..7472
                     /label=ATG9A
     protein_bind    complement(7474..7498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7517..8016
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             8040..8438
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8480..8601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8627..9215
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9098..9109)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9290..9523
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        9728..9832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9833..10693
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tggagttccg
     3601 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3661 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3721 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3781 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3841 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3901 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3961 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4021 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4081 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     4141 ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgatctgcc accatggtga
     4201 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     4261 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4321 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4381 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4441 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4501 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4561 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4621 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4681 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4741 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4801 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4861 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gctcgaggtt
     4921 atcaaacaag tttgtacaaa aaagcaggct taatggcgca gtttgacact gaataccagc
     4981 gcctagaggc ctcctatagt gattcacccc caggggagga ggacctgttg gtgcacgtcg
     5041 ccgaggggag caagtcacct tggcaccata ttgaaaacct tgacctcttc ttctctcgag
     5101 tttataatct gcaccagaag aatggcttca catgtatgct catcggggag atctttgagc
     5161 tcatgcagtt cctctttgtg gttgccttca ctaccttcct ggtcagctgc gtggactatg
     5221 acatcctatt tgccaacaag atggtgaacc acagtcttca ccctactgaa cccgtcaagg
     5281 tcactctgcc agacgccttt ttgcctgctc aagtctgtag tgccaggatt caggaaaatg
     5341 gctcccttat caccatcctg gtcattgctg gtgtcttctg gatccaccgg cttatcaagt
     5401 tcatctataa catttgctgc tactgggaga tccactcctt ctacctgcac gctctgcgca
     5461 tccctatgtc tgcccttccg tattgcacgt ggcaagaagt gcaggcccgg atcgtgcaga
     5521 cgcagaagga gcaccagatc tgcatccaca aacgtgagct gacagaactg gacatctacc
     5581 accgcatcct ccgtttccag aactacatgg tggcactggt taacaaatcc ctcctgcctc
     5641 tgcgcttccg cctgcctggc ctcggggaag ctgtcttctt cacccgtggt ctcaagtaca
     5701 actttgagct gatcctcttc tggggacctg gctctctgtt tctcaatgaa tggagcctca
     5761 aggccgagta caaacgtggg gggcaacggc tagagctggc ccagcgcctc agcaaccgca
     5821 tcctgtggat tggcatcgct aacttcctgc tgtgccccct catcctcata tggcaaatcc
     5881 tctatgcctt cttcagctat gctgaggtgc tgaagcggga gccgggggcc ctgggagcac
     5941 gctgctggtc actctatggc cgctgctacc tccgccactt caacgagctg gagcacgagc
     6001 tgcagtcccg cctcaaccgt ggctacaagc ccgcctccaa gtacatgaat tgcttcttgt
     6061 cacctctttt gacactgctg gccaagaatg gagccttctt cgctggctcc atcctggctg
     6121 tgcttattgc cctcaccatt tatgacgaag atgtgttggc tgtggaacat gtgctgacca
     6181 ccgtcacact cctgggggtc accgtgaccg tgtgcaggtc ctttatcccg gaccagcaca
     6241 tggtgttctg ccctgagcag ctgctccgcg tgatcctcgc tcacatccac tacatgcctg
     6301 accactggca gggtaatgcc caccgctcgc agacccggga cgagtttgcc cagctcttcc
     6361 agtacaaggc agtgttcatt ttggaagagt tgctgagccc cattatcaca cccctcatcc
     6421 tcatcttctg cctgcgccca cgggccctgg agattataga cttcttccga aacttcaccg
     6481 tggaggtcgt tggtgtggga gatacctgct cctttgctca gatggatgtt cgccagcatg
     6541 gtcatcccca gtggctatct gctgggcaga cagaggcctc agtgtaccag caagctgagg
     6601 atggaaagac agagttgtca ctcatgcact ttgccatcac caaccctggc tggcagccac
     6661 cacgtgagag cacagccttc ctaggcttcc tcaaggagca ggttcagcgg gatggagcag
     6721 ctgctggcct cgcccaaggg ggtctgctcc ctgaaaatgc cctctttacg tctatccagt
     6781 ccttacaatc tgagtctgag cccctgagcc ttatcgcaaa tgtggtagct ggctcatcct
     6841 gccggggccc tccactgccc agagacctgc agggctccag gcacagggct gaagtcgcct
     6901 ctgccctgcg ctccttctcc ccgctgcaac ccgggcaggc gcccacaggc cgggctaaca
     6961 gcaccatgac aggctctggg gtggatgcca ggacagccag ctccgggagc agcgtgtggg
     7021 aaggacagct gcagagcctg gtgctgtcag aatatgcatc cacagagatg agcctgcatg
     7081 ccctctatat gcaccagctc cacaagcagc aggcccaggc tgaacctgag cggcatgtat
     7141 ggcaccgccg ggagagtgat gagagtggag aaagcgcccc tgatgaaggg ggagagggcg
     7201 cccgggcccc ccagtctatc cctcgctctg ctagctatcc ctgtgcagca ccccggcctg
     7261 gagctcctga gaccaccgcc ctgcatgggg gcttccagag gcgctacggt ggcatcacag
     7321 atcctggcac agtgcccagg gttccctctc atttctctcg gctgcctctt ggagggtggg
     7381 cagaagatgg gcagtcggca tcaaggcacc ctgagcccgt gcccgaagag ggctcggagg
     7441 atgagctacc ccctcaggtg cacaaggtat agaacccagc tttcttgtac aaagtggttc
     7501 gataacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     7561 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     7621 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     7681 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     7741 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     7801 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     7861 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     7921 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     7981 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccatt aattaaacca
     8041 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
     8101 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
     8161 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
     8221 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
     8281 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
     8341 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
     8401 gtgaattgct gccctctggt tatgtgtggg agggctaagc acttcgtggc cgaggagcag
     8461 gactgacact cgacctcgaa acttgtttat tgcagcttat aatggttaca aataaagcaa
     8521 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8581 caaactcatc aatgtatctt atcatgtcta tgcatcgata gatcctaatc aacctctgga
     8641 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8701 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8761 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8821 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8881 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8941 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     9001 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     9061 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     9121 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     9181 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     9241 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     9301 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     9361 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9421 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9481 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9541 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     9601 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     9661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
    10081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10681 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10741 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10861 ttc
//
