LOCUS       pCK530_DDX4             8692 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    387..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..398
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            399..467
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    399..1136
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             399..2573
                     /label="DDX4 CDS (probable ATP-dependent RNA helicase DDX4 isoform 1)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             399..2573
                     /label="Translation 399-2573"
                     /translation="MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDAGECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSEASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGGYKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHMMHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKGKSTLNTAGFSSSQAPNPVDDESWD*"
     exon            468..525
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     exon            526..603
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            604..681
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            682..732
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            733..792
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          774..796
                     /label="q059R"
                     /note="sequence: GGGTTTTCCAAGAGAGGCGGCTA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            793..894
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(855..875)
                     /label="q059F"
                     /note="sequence: TCCTCCACGGCAACCTCGGAA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     exon            895..975
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            976..1023
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     exon            1024..1071
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1062..1064
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            1072..1119
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1074..1076
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1080..1139
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            1120..1262
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1260..1346
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            1263..1415
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     exon            1416..1570
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            1571..1700
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            1701..1867
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1734..1745
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     exon            1868..2013
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2001..2020
                     /label="q058F"
                     /note="sequence: CTGCGAAACATAGGGGATGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            2014..2113
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2113..2133)
                     /label="q058R"
                     /note="sequence: GCTCTCTCTGTTCCCGATCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     exon            2114..2384
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2163..2184
                     /label="MPS007_DDX4_geno_5'_fwd"
                     /note="sequence: GGAAAGTGCCCAGTTCTTGTTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2277..2307
                     /label="MPS001_DDX4_5'_fwd"
                     /note="sequence: CGAATTGGGCGTACTGGTCGTTGTGGGAATA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            2385..2495
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            2496..2573
                     /label="DDX4 exon"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2508..2570
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2538..2570)
                     /label="MPS003_DDX4_5'arm_rev"
                     /note="sequence: ATCCCATGACTCATCATCTACTGGATTGGGAGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2562..2564
                     /label="DDX4 misc_feature"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(2577..2595)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2581..2597)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2581..2598)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2581..2661
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2586..2595
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2600..2626
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2600..2626)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2627..2635
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2633..2661
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2636..2661)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2639..2661
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     2639..2661
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2639..2661
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2662..2731
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2666..2688
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2689..2708
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_difference complement(2709..2731)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2732..2735
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2736..2758)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2736..2758)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2736..2758)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2736..2767
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2759..2783
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2768..2801
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2789..2810)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2793..2927
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2802..2957
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2958..5668
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3199..3233)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3245..3260)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3469..3488)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3590..3612
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(3651..3669)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3725..3744
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3737..3841
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3842..4702
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4060..4079)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4060..4079)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4873..5461
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5362..5381
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5615..5632
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5669..5757
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5758..8626
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5838..5859
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5874..5904
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5912..5928
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     5936..5952
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5978..6040
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6216..6297
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6324..6343
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6390..6413)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6414..7013)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6899..6918
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          7014..7031
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7014..7070)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(7080..7823)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7221..7240)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7424..7443
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     7466..7485
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7520..7539)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7824..7847)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7847..7856)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7876..8087)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(8088..8467)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8616..8635
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8627..8659
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8660..8692
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8669..8687)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTGCCACCat gggagatgaa gattgggaag
      421 cagaaatcaa ccctcatatg tcttcctatg ttcccatatt tgagaaggat aggtattctg
      481 gagaaaatgg agacaatttt aacaggactc cagcttcatc atcagaaatg gatgatggac
      541 cttctcgaag agatcatttc atgaaaagtg gatttgcctc tgggcggaat tttggaaaca
      601 gagatgctgg tgagtgtaat aagcgagata atacatccac aatgggtggt tttggagttg
      661 gaaagagttt tggaaacaga ggtttttcaa acagcaggtt tgaagatggt gatagctctg
      721 gtttctggag agagtctagt aatgactgcg aagataatcc aacacggaac agagggtttt
      781 ccaagagagg cggctatcga gatggaaata attcagaagc ttcagggcca tacagaagag
      841 gtggaagagg tagtttccga ggttgccgtg gaggatttgg tctaggaagt ccaaataatg
      901 acttagaccc agacgaatgt atgcagcgca ctggtggcct ttttggttct agaagaccag
      961 tattaagtgg cacaggtaat ggtgatactt ctcaaagcag aagtggcagt ggaagtgaac
     1021 gaggtggtta caaaggttta aatgaagaag taataacagg ctctggaaag aattcttgga
     1081 agtcagaagc agaaggagga gaaagtagtg atactcaagg accaaaagtg acctacatac
     1141 cccctcctcc acctgaggat gaggactcca tctttgcaca ttatcagaca ggcataaact
     1201 tcgacaaata cgacactatt cttgtggaag tgtctggaca tgatgcacca ccagcaattc
     1261 tgacttttga agaagctaat ctctgtcaga cactgaataa caacattgct aaagctggtt
     1321 atactaagct tactcctgtg caaaaataca gtattcctat catacttgca ggacgagatt
     1381 tgatggcttg cgctcaaaca gggtctggga agactgcggc ttttctccta ccaattttgg
     1441 ctcatatgat gcatgatgga ataactgcca gtcgttttaa agagttgcag gaaccagagt
     1501 gtattattgt agcaccaact cgagaattgg tcaaccagat ttatttggaa gccagaaaat
     1561 tttcttttgg gacttgtgta agagctgttg ttatatatgg gggaacccag ctgggacatt
     1621 caattcgaca aatagtacaa ggctgtaata tattatgtgc tactcctgga agactgatgg
     1681 atatcatagg caaagaaaag attggtctca aacagatcaa atacttagtt ttggatgaag
     1741 ctgatcgcat gttggatatg ggttttggtc cagaaatgaa gaagttaatt tcttgcccag
     1801 gaatgccatc aaaggaacag cgccaaaccc ttatgttcag tgcaactttt ccagaggaaa
     1861 ttcaaaggtt ggctgcagag tttttaaagt caaattatct gtttgttgct gttggacaag
     1921 tgggtggagc atgtagagat gttcagcaga ccgttctcca agttggccag ttctcaaaaa
     1981 gagaaaagct cgttgaaatt ctgcgaaaca taggggatga aagaactatg gtctttgttg
     2041 aaactaagaa aaaagcagat tttattgcaa cttttctttg tcaagaaaaa atatcaacta
     2101 caagtattca tggtgatcgg gaacagagag agcgggagca agctcttgga gattttcgct
     2161 ttggaaagtg cccagttctt gttgctactt cagtagctgc cagagggctg gatattgaaa
     2221 atgtgcaaca tgttatcaat tttgatcttc cttctaccat tgatgaatat gttcatcgaa
     2281 ttgggcgtac tggtcgttgt gggaatactg gcagagcaat ttcctttttt gatcttgaat
     2341 cggataacca tttagcacag cctctagtaa aagtattgac agatgctcaa caggatgttc
     2401 ctgcatggtt ggaagaaatt gcctttagta catacattcc tggcttcagt ggtagtacaa
     2461 gaggaaacgt gtttgcatca gttgatacca gaaagggcaa gagcactttg aacacagctg
     2521 ggttttcttc ttcacaagct cccaatccag tagatgatga gtcatgggat taaACCCAGC
     2581 TTTCTTGTAC AAAGTGGTAT ACCCATACGA TGTTCCAGAT TACGCTTAGT AATGAGAAAT
     2641 ACTCAGAAGA TGTCACTCAC CATAGTCCGC AGTGAAAGAT AAATGATCCA TAAGCTTCCT
     2701 TGTCCTACGT TTTAGAGCTA GAAATAGCAA GTAGGCCACT GAGCAAGAAC TATGAATGAG
     2761 ATCTTTAGAC ATATTGCGGC CGCTTTCTCC GCGATCCAGA CATGATAAGA TACATTGATG
     2821 AGTTTGGACA AACCAAAACT AGAATGCAGT GAAAAAAATG CCTTATTTGT GAAATTTGTG
     2881 ATGCTATTGC CTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTATC TATAACAAGA
     2941 AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT
     3001 ATGAGTTAAA TCTTAAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT
     3061 CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC
     3121 AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG
     3181 AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAAGAA
     3241 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
     3301 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
     3361 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
     3421 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
     3481 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
     3541 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCATGTCTC CGGGAGCTGC
     3601 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACACGCGA GTTCGAAAGG GCCTCGTGAT
     3661 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC
     3721 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
     3781 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
     3841 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
     3901 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
     3961 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
     4021 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
     4081 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
     4141 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
     4201 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
     4261 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
     4321 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
     4381 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
     4441 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
     4501 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGTTC
     4561 ACGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
     4621 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
     4681 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
     4741 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT
     4801 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     4861 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     4921 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     4981 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT
     5041 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     5101 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     5161 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     5221 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC
     5281 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     5341 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     5401 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     5461 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
     5521 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC
     5581 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA
     5641 TGAGCGCCCA ATACGCAAAC CGCCTCTCAC CGCGCGTTGG CCGATTCATT AATGCAGCTG
     5701 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAAACC GCCTCTCCCC
     5761 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
     5821 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
     5881 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
     5941 AACAGCTATG ACCATGATTA CGCCAAGGTC GACTTAACCC TAGAAAGATA ATCATATTGT
     6001 GACGTACGTT AAAGATAATC ATGCGTAAAA TTGACGCATG TGTTTTATCG GTCTGTATAT
     6061 CGAGGTTTAT TTATTAATTT GAATAGATAT TAAGTTTTAT TATATTTACA CTTACATACT
     6121 AATAATAAAT TCAACAAACA ATTTATTTAT GTTTATTTAT TTATTAAAAA AAAACAAAAA
     6181 CTCAAAATTT CTTCTATAAA GTAACAAAAC TTTTAGCAGT GAAAAAAATG CTTTATTTGT
     6241 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
     6301 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
     6361 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTGGA GATTCATGCG
     6421 CCAGGTTTTC TGGTCATGCA CCACGTTCGT GGGCCCTCAG GAACCTCCAC GTCAGCGGTA
     6481 ACTGTGAAGC CCAGTCGCTC GTAAAAAGGA AGATTCCGGG GAGCACTGGT TTCGAGGAAC
     6541 GCCGGCACGC CAGCCCGTTC CGCGGCCTCA ACACCAGGAA GAACGACAGC GCTTCCCAAA
     6601 CCTTTACCTT GGTGATCTGG GCTGACACCG ACAGTGGCAA GAAACCACGC AGGCTCCTTT
     6661 GGTCTATGAG GAGCCAGCAA GCCCTCCATT TGCTGTTGTG CCGCCAACCT ACTCCCTGAC
     6721 AGCTCGGCCA TCCGCGGACC TATTTCCGCG AACACTGCGC CGGCTTCGAC GCTTTCCGGA
     6781 GTAGTCCAGA CCGCAACTGC GGCACCATCG TCCGCAACCC AAACTTTACC AATGTCCAGA
     6841 CCCACGCGCG TAAGAAACAG CTCTTGCAAC TCAGTTACCC TCTCTATGTG TCTGTCCGGA
     6901 TCTACGGTAT GGCGGGTCGC TGGATAATCT GCAAATGCTG CGGCAAGTGT TCGGACAGCC
     6961 CGTGGTACAT CGTCACGGGT AGCGAGCCTA ACAGTGGGCT TATATTCCGT CATGGGGCCG
     7021 GGGTTCTCCT CCACGTCGCC GGCCTGCTTC AGCAGGCTGA AGTTGGTGGC GCCGCTGCCC
     7081 CCGGGGAGCA TGTCAAGGTC AAAATCGTCA AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA
     7141 AAGTCGTCAA GGGCATCGGC TGGGAGCATG TCTAAGTCAA AATCGTCAAG GGCGTCGGTC
     7201 GGCCCGCCGC TTTCGCACTT TAGCTGTTTC TCCAGGCCAC ATATGATTAG TTCCAGGCCG
     7261 AAAAGGAAGG CAGGTTCGGC TCCCTGCCGG TCGAACAGCT CAATTGCTTG TTTCAGAAGT
     7321 GGGGGCATAG AATCGGTGGT AGGTGTCTCT CTTTCCTCTT TTGCTACTTG ATGCTCCTGT
     7381 TCCTCCAATA CGCAGCCCAG TGTAAAGTGG CCCACGGCGG ACAGAGCGTA CAGTGCGTTC
     7441 TCCAGGGAGA AGCCTTGCTG ACACAGGAAC GCGAGCTGAT TTTCCAGGGT TTCGTACTGT
     7501 TTCTCTGTTG GGCGGGTGCC GAGATGCACT TTAGCCCCGT CACGATGTGA GAGGAGAGCA
     7561 CAGCGGTATG ACTTGGCGTT GTTCCGCAGA AAGTCTTGCC ATGACTCGCC TTCCAGGGGG
     7621 CAGGAGTGGG TATGATGCCT GTCCAGCATC TCGATTGGCA GGGCATCGAG CAGGGCCCGC
     7681 TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC TGCTCAACTC CCAGCTTTTG AGCGAGTTTC
     7741 CTTGTCGTCA GGCCTTCGAT ACCGACTCCA TTGAGTAATT CCAGAGCAGA GTTTATGACT
     7801 TTGCTCTTGT CCAGTCTAGA CATCTTATCG TCATCGTCTT TGTAATCCAT GGTGGCGGAT
     7861 CCCGCGTCAC GACACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAAGAA CGTTCACGGC
     7921 GACTACTGCA CTTATATACG GTTCTCCCCC ACCCTCGGGA AAAAGGCGGA GCCAGTACAC
     7981 GACATCACTT TCCCAGTTTA CCCCGCGCCA CCTTCTCTAG GCACCGGTTC AATTGCCGAC
     8041 CCCTCCCCCC AACTTCTCGG GCACTGTGGG CCATGTGCGC TCTGCCCCAT GGTAATAGCC
     8101 ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTGCC ATAAGGTCAT GTACTGGGCA
     8161 TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA
     8221 TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA
     8281 TGGAAAGTGC CTATTGGCGT TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG
     8341 GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC
     8401 ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT
     8461 CAATGTCATA AGCTCTAATC GCATAATCGA GCTAAGAGCG ATTAATATGC TTAGCTAGCT
     8521 TAGCTAAGCT AGATTTCTCT ATTCTCCCAC AATATATAGC AGCTATTAGC TTAGCAGTCC
     8581 GAATACGATC GTCCACGATC CATAAGCGTA ACACATTTGA GTAGCGACTG ACGGGCACCG
     8641 GAGCCACTCG AGTGGAATTA CTGACGGGCA CCGGAGCCAC TCGAGTGGAA TT
//