LOCUS       CK530_35_TF101_EZH2     8776 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..2653
                     /label="EZH2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             398..2653
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP*"
     CDS             2654..2719
                     /label="Translation 2654-2719"
                     /translation="LDPAFLYKVVYPYDVPDYA***"
     misc_feature    2658..2679
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2658..2681)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2661..2679)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2665..2681)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2665..2682)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2665..2745
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2670..2679
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2684..2710
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2684..2710)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2711..2719
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2717..2745
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2720..2745)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2723..2745
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     2723..2745
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2723..2745
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2746..2815
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2750..2772
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2773..2792
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_difference complement(2793..2815)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2816..2819
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2820..2842)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2820..2842)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2820..2842)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2820..2851
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2843..2867
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2852..2885
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2873..2894)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2877..3011
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2886..3041
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 3042..5752
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3283..3317)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3329..3344)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3329..3344)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3348..3364)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3553..3572)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3674..3696
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3674..3696
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3735..3753)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3809..3828
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3821..3925
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3926..4786
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4144..4163)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4144..4163)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4957..5545
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5446..5465
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5699..5716
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5753..5841
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5842..8710
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5922..5943
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5958..5988
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5996..6012
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          6003..6022
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     6020..6036
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   6062..6124
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6300..6381
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6408..6427
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6415..6431
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6462..6481
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6474..6497)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6498..7097)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6983..7002
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          7098..7115
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7098..7154)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(7164..7907)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7305..7324)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7508..7527
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     7550..7569
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7604..7623)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7908..7931)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7931..7940)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7960..8171)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(8172..8551)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8700..8719
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8711..8743
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8744..8776
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8753..8771)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg ggccagactg ggaagaaatc
      421 tgagaaggga ccagtttgtt ggcggaagcg tgtaaaatca gagtacatgc gactgagaca
      481 gctcaagagg ttcagacgag ctgatgaagt aaagagtatg tttagttcca atcgtcagaa
      541 aattttggaa agaacggaaa tcttaaacca agaatggaaa cagcgaagga tacagcctgt
      601 gcacatcctg acttctgtga gctcattgcg cgggactagg gagtgttcgg tgaccagtga
      661 cttggatttt ccaacacaag tcatcccatt aaagactctg aatgcagttg cttcagtacc
      721 cataatgtat tcttggtctc ccctacagca gaattttatg gtggaagatg aaactgtttt
      781 acataacatt ccttatatgg gagatgaagt tttagatcag gatggtactt tcattgaaga
      841 actaataaaa aattatgatg ggaaagtaca cggggataga gaatgtgggt ttataaatga
      901 tgaaattttt gtggagttgg tgaatgccct tggtcaatat aatgatgatg acgatgatga
      961 tgatggagac gatcctgaag aaagagaaga aaagcagaaa gatctggagg atcaccgaga
     1021 tgataaagaa agccgcccac ctcggaaatt tccttctgat aaaatttttg aagccatttc
     1081 ctcaatgttt ccagataagg gcacagcaga agaactaaag gaaaaatata aagaactcac
     1141 cgaacagcag ctcccaggcg cacttcctcc tgaatgtacc cccaacatag atggaccaaa
     1201 tgctaaatct gttcagagag agcaaagctt acactccttt catacgcttt tctgtaggcg
     1261 atgttttaaa tatgactgct tcctacatcg taagtgcaat tattcttttc atgcaacacc
     1321 caacacttat aagcggaaga acacagaaac agctctagac aacaaacctt gtggaccaca
     1381 gtgttaccag catttggagg gagcaaagga gtttgctgct gctctcaccg ctgagcggat
     1441 aaagacccca ccaaaacgtc caggaggccg cagaagagga cggcttccca ataacagtag
     1501 caggcccagc acccccacca ttaatgtgct ggaatcaaag gatacagaca gtgataggga
     1561 agcagggact gaaacggggg gagagaacaa tgataaagaa gaagaagaga agaaagatga
     1621 aacttcgagc tcctctgaag caaattctcg gtgtcaaaca ccaataaaga tgaagccaaa
     1681 tattgaacct cctgagaatg tggagtggag tggtgctgaa gcctcaatgt ttagagtcct
     1741 cattggcact tactatgaca atttctgtgc cattgctagg ttaattggga ccaaaacatg
     1801 tagacaggtg tatgagttta gagtcaaaga atctagcatc atagctccag ctcccgctga
     1861 ggatgtggat actcctccaa ggaaaaagaa gaggaaacac cggttgtggg ctgcacactg
     1921 cagaaagata cagctgaaaa aggacggctc ctctaaccat gtttacaact atcaaccctg
     1981 tgatcatcca cggcagcctt gtgacagttc gtgcccttgt gtgatagcac aaaatttttg
     2041 tgaaaagttt tgtcaatgta gttcagagtg tcaaaaccgc tttccgggat gccgctgcaa
     2101 agcacagtgc aacaccaagc agtgcccgtg ctacctggct gtccgagagt gtgaccctga
     2161 cctctgtctt acttgtggag ccgctgacca ttgggacagt aaaaatgtgt cctgcaagaa
     2221 ctgcagtatt cagcggggct ccaaaaagca tctattgctg gcaccatctg acgtggcagg
     2281 ctgggggatt tttatcaaag atcctgtgca gaaaaatgaa ttcatctcag aatactgtgg
     2341 agagattatt tctcaagatg aagctgacag aagagggaaa gtgtatgata aatacatgtg
     2401 cagctttctg ttcaacttga acaatgattt tgtggtggat gcaacccgca agggtaacaa
     2461 aattcgtttt gcaaatcatt cggtaaatcc aaactgctat gcaaaagtta tgatggttaa
     2521 cggtgatcac aggataggta tttttgccaa gagagccatc cagactggcg aagagctgtt
     2581 ttttgattac agatacagcc aggctgatgc cctgaagtat gtcggcatcg aaagagaaat
     2641 ggaaatccct tgaTTGGACC CAGCTTTCTT GTACAAAGTG GTATACCCAT ACGATGTTCC
     2701 AGATTACGCT TAGTAATGAG AAATACTCAG AAGATGTCAC TCACCATAGT CCGCAGTGAA
     2761 AGATAAATGA TCGGCAACGC ACTTGACGCC AGGTTTTAGA GCTAGAAATA GCAAGTAGGC
     2821 CACTGAGCAA GAACTATGAA TGAGATCTTT AGACATATTG CGGCCGCTTT CTCCGCGATC
     2881 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAA AACTAGAATG CAGTGAAAAA
     2941 AATGCCTTAT TTGTGAAATT TGTGATGCTA TTGCCTTATT TGTAACCATT ATAAGCTGCA
     3001 ATAAACAAGT TATCTATAAC AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC
     3061 ATGAAATAAC AATATAATTA TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC
     3121 ATGCGTCATT TTGACTCACG CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG
     3181 ACAAGCACGC CTCACGGGAG CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG
     3241 GATTCGCGCT ATTTAGAAAG AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC
     3301 AGACTATCTT TCTAGGGTTA AGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA
     3361 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG
     3421 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA
     3481 ATGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG
     3541 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC
     3601 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC
     3661 TGTGACCATG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACAC
     3721 GCGAGTTCGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
     3781 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
     3841 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
     3901 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
     3961 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
     4021 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
     4081 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
     4141 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
     4201 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
     4261 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
     4321 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
     4381 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC
     4441 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT
     4501 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA
     4561 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA
     4621 ATCTGGAGCC GGTGAGCGTG GTTCACGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
     4681 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA
     4741 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
     4801 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT
     4861 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
     4921 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
     4981 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
     5041 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
     5101 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
     5161 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
     5221 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
     5281 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
     5341 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
     5401 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
     5461 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
     5521 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
     5581 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
     5641 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
     5701 CGAGTCAGTG AGCGAGGAAG CGGATGAGCG CCCAATACGC AAACCGCCTC TCACCGCGCG
     5761 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
     5821 GCGCAACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC
     5881 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT
     5941 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG
     6001 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GGTCGACTTA
     6061 ACCCTAGAAA GATAATCATA TTGTGACGTA CGTTAAAGAT AATCATGCGT AAAATTGACG
     6121 CATGTGTTTT ATCGGTCTGT ATATCGAGGT TTATTTATTA ATTTGAATAG ATATTAAGTT
     6181 TTATTATATT TACACTTACA TACTAATAAT AAATTCAACA AACAATTTAT TTATGTTTAT
     6241 TTATTTATTA AAAAAAAACA AAAACTCAAA ATTTCTTCTA TAAAGTAACA AAACTTTTAG
     6301 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
     6361 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
     6421 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
     6481 TATGATCCTC TGGAGATTCA TGCGCCAGGT TTTCTGGTCA TGCACCACGT TCGTGGGCCC
     6541 TCAGGAACCT CCACGTCAGC GGTAACTGTG AAGCCCAGTC GCTCGTAAAA AGGAAGATTC
     6601 CGGGGAGCAC TGGTTTCGAG GAACGCCGGC ACGCCAGCCC GTTCCGCGGC CTCAACACCA
     6661 GGAAGAACGA CAGCGCTTCC CAAACCTTTA CCTTGGTGAT CTGGGCTGAC ACCGACAGTG
     6721 GCAAGAAACC ACGCAGGCTC CTTTGGTCTA TGAGGAGCCA GCAAGCCCTC CATTTGCTGT
     6781 TGTGCCGCCA ACCTACTCCC TGACAGCTCG GCCATCCGCG GACCTATTTC CGCGAACACT
     6841 GCGCCGGCTT CGACGCTTTC CGGAGTAGTC CAGACCGCAA CTGCGGCACC ATCGTCCGCA
     6901 ACCCAAACTT TACCAATGTC CAGACCCACG CGCGTAAGAA ACAGCTCTTG CAACTCAGTT
     6961 ACCCTCTCTA TGTGTCTGTC CGGATCTACG GTATGGCGGG TCGCTGGATA ATCTGCAAAT
     7021 GCTGCGGCAA GTGTTCGGAC AGCCCGTGGT ACATCGTCAC GGGTAGCGAG CCTAACAGTG
     7081 GGCTTATATT CCGTCATGGG GCCGGGGTTC TCCTCCACGT CGCCGGCCTG CTTCAGCAGG
     7141 CTGAAGTTGG TGGCGCCGCT GCCCCCGGGG AGCATGTCAA GGTCAAAATC GTCAAGAGCG
     7201 TCAGCAGGCA GCATATCAAG GTCAAAGTCG TCAAGGGCAT CGGCTGGGAG CATGTCTAAG
     7261 TCAAAATCGT CAAGGGCGTC GGTCGGCCCG CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG
     7321 CCACATATGA TTAGTTCCAG GCCGAAAAGG AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC
     7381 AGCTCAATTG CTTGTTTCAG AAGTGGGGGC ATAGAATCGG TGGTAGGTGT CTCTCTTTCC
     7441 TCTTTTGCTA CTTGATGCTC CTGTTCCTCC AATACGCAGC CCAGTGTAAA GTGGCCCACG
     7501 GCGGACAGAG CGTACAGTGC GTTCTCCAGG GAGAAGCCTT GCTGACACAG GAACGCGAGC
     7561 TGATTTTCCA GGGTTTCGTA CTGTTTCTCT GTTGGGCGGG TGCCGAGATG CACTTTAGCC
     7621 CCGTCACGAT GTGAGAGGAG AGCACAGCGG TATGACTTGG CGTTGTTCCG CAGAAAGTCT
     7681 TGCCATGACT CGCCTTCCAG GGGGCAGGAG TGGGTATGAT GCCTGTCCAG CATCTCGATT
     7741 GGCAGGGCAT CGAGCAGGGC CCGCTTGTTC TTCACGTGCC AGTACAGGGT AGGCTGCTCA
     7801 ACTCCCAGCT TTTGAGCGAG TTTCCTTGTC GTCAGGCCTT CGATACCGAC TCCATTGAGT
     7861 AATTCCAGAG CAGAGTTTAT GACTTTGCTC TTGTCCAGTC TAGACATCTT ATCGTCATCG
     7921 TCTTTGTAAT CCATGGTGGC GGATCCCGCG TCACGACACC TGTGTTCTGG CGGCAAACCC
     7981 GTTGCGAAAA AGAACGTTCA CGGCGACTAC TGCACTTATA TACGGTTCTC CCCCACCCTC
     8041 GGGAAAAAGG CGGAGCCAGT ACACGACATC ACTTTCCCAG TTTACCCCGC GCCACCTTCT
     8101 CTAGGCACCG GTTCAATTGC CGACCCCTCC CCCCAACTTC TCGGGCACTG TGGGCCATGT
     8161 GCGCTCTGCC CCATGGTAAT AGCCATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG
     8221 TGCCATAAGG TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCA
     8281 ATAGGGGGCG TACTTGGCAT ATGATACACT TGATGTACTG CCAAGTGGGC AGTTTACCGT
     8341 AAATACTCCA CCCATTGACG TCAATGGAAA GTGCCTATTG GCGTTACTAT GGGAACATAC
     8401 GTCATTATTG ACGTCAATGG GCGGGGGTCG TTGGGCGGTC AGCCAGGCGG GCCATTTACC
     8461 GTAAGTTATG TAACGCGGAA CTCCATATAT GGGCTATGAA CTAATGACCC CGTAATTGAT
     8521 TACTATTAAT AACTAGTCAA TAATCAATGT CATAAGCTCT AATCGCATAA TCGAGCTAAG
     8581 AGCGATTAAT ATGCTTAGCT AGCTTAGCTA AGCTAGATTT CTCTATTCTC CCACAATATA
     8641 TAGCAGCTAT TAGCTTAGCA GTCCGAATAC GATCGTCCAC GATCCATAAG CGTAACACAT
     8701 TTGAGTAGCG ACTGACGGGC ACCGGAGCCA CTCGAGTGGA ATTACTGACG GGCACCGGAG
     8761 CCACTCGAGT GGAATT
//