LOCUS       CK530_36_TF102_KCTD19-H 9292 bp ds-DNA     circular     09-OCT-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     misc_feature    1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..386
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..394
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    395..3175
                     /label="KCTD19"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             395..3235
                     /label="Translation 395-3235"
                     /translation="MEESGMAHESAEDLFHFNVGGWHFSVPRSKLSQFPDSLLWKEASALTSSESQRLFIDRDGSTFRHVHYYLYTSKLSFSSCAELNLLYEQALGLQLMPLLQTLDNLKEGKHHLRVRPADLPVAERASLNYWRTWKCISKPSEFPIKSPAFTGLHDKAPLGLMDTPLLDTEEEVHYCFLPLDLVAKYPSLVTEDNLLWLAETVALIECECSEFRFIVNFLRSQKILLPDNFSNIDVLEAEVEILEIPALTEAVRWYRMNMGGCSPTTCSPLSPGKGARTASLESVKPLYTMALGLLVKYPDSALGQLRIESTLDGSRLYITGNGVLFQHVKNWLGTCRLPLTETISEVYELCAFLDKRDITYEPIKVALKTHLEPRTLAPMDVLNEWTAEITVYSPQQIIKVYVGSHWYATTLQTLLKYPELLSNPQRVYWITYGQTLLIHGDGQMFRHILNFLRLGKLFLPSEFKEWPLFCQEVEEYHIPSLSEALAQCEAYKSWTQEKESENEEAFSIRRLHVVTEGPGSLVEFSRDTKETTAYMPVDFEDCSDRTPWNKAKGNLVRSNQMDEAEQYTRPIQVSLCRNAKRAGNPSTYSHCRGLCTNPGHWGSHPESPPKKKCTTINLTQKSETKDPPATPMQKLISLVREWDMVNCKQWEFQPLTATRSSPLEEATLQLPLGSEAASQPSTSAAWKAHSTASEKDPGPQAGAGAGAKDKGPEPTFKPYLPPKRAGTLKDWSKQRTKERESPAPEQPLPEASEVDSLGVILKVTHPPVVGSDGFCMFFEDSIIYTTEMDNLRHTTPTASPQPQEVTFLSFSLSWEEMFYAQKCHCFLADIIMDSIRQKDPKAITAKVVSLANRLWTLHISPKQFVVDLLAITGFKDDRHTQERLYSWVELTLPFARKYGRCMDLLIQRGLSRSVSYSILGKYLQEDLPTFLYKVVYPYDVPDYA***"
     misc_feature    3176..3195
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3177..3195)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3181..3197)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(3181..3198)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3181..3261
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3186..3195
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3200..3226
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(3200..3226)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      3227..3235
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3233..3261
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3236..3261)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     3239..3261
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     3239..3261
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3239..3261
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3262..3331
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 3266..3288
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3289..3308
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_difference complement(3309..3331)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3332..3335
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3336..3358)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3336..3358)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3336..3358)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3336..3367
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3359..3383
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3368..3401
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3389..3410)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    3393..3527
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3402..3557
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3558..6268
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3799..3833)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3845..3860)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3845..3860)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3864..3880)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(4069..4088)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     4190..4212
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4190..4212
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(4251..4269)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4325..4344
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        4337..4441
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4442..5302
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4660..4679)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4660..4679)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      5473..6061
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5962..5981
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6215..6232
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6269..6357
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6358..9226
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    6438..6459
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        6474..6504
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    6512..6528
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          6519..6538
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     6536..6552
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   6578..6640
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6816..6897
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6924..6943
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6931..6947
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6978..6997
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6990..7013)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(7014..7613)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     7499..7518
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          7614..7631
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7614..7670)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(7680..8423)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7821..7840)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          8024..8043
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     8066..8085
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(8120..8139)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(8424..8447)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(8447..8456)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(8476..8687)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(8688..9067)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     9216..9235
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    9227..9259
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    9260..9292
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(9269..9287)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGttgg CACCatggag gagtctggca tggctcatga
      421 atcagcagag gacttgtttc atttcaacgt agggggctgg catttctcag ttcccagaag
      481 caaactctct cagtttccag actccctgct gtggaaagag gcttcagcct tgacctcttc
      541 agaaagccag aggctattta tcgacagaga tggttccaca tttaggcacg tgcactatta
      601 cctctacacc tccaaactct ccttctccag ttgtgcagaa ctgaacttgc tgtatgagca
      661 agcattgggt ttgcagctga tgcctttgct gcagactcta gataacctga aggaagggaa
      721 acaccatcta cgcgtacggc ctgcagacct acctgttgct gagagagcat ctctgaacta
      781 ctggcgtaca tggaagtgta ttagcaaacc ctcagaattt ccaattaaaa gcccagcctt
      841 tacaggccta catgataagg cacctctggg gctcatggac acacccctgt tagacacaga
      901 agaggaggtg cactactgct tcctgcccct agacctggtg gccaaatatc ccagcctagt
      961 gactgaagac aacctgctgt ggctggctga gacggtggcc ctcatcgagt gcgagtgcag
     1021 cgagttccgc ttcattgtga attttcttcg ctcacagaag attttactac cggataattt
     1081 ctccaacatt gatgtattag aagcagaagt ggaaattctg gaaatccctg cactcactga
     1141 agccgtaagg tggtaccgga tgaacatggg tggctgttcc ccgaccacct gttctcccct
     1201 gagccccggg aagggggccc gcacagccag cctggagtcc gtgaaaccgc tctacacaat
     1261 ggccctgggt ctgctggtca agtacccgga ctctgcgctg ggccagcttc gcatcgagag
     1321 cacgctagac ggaagccgac tgtacatcac agggaatggc gtcctctttc agcacgtcaa
     1381 gaactggctg gggacttgcc ggctgcccct gacagagacc atttccgagg tatatgagct
     1441 ctgtgccttc ctagacaaaa gggacatcac ctacgagcca atcaaagttg ctttgaagac
     1501 tcatctggag ccaaggactt tggcacccat ggatgtgctc aatgagtgga cggcagagat
     1561 cactgtgtat tccccacaac agatcatcaa agtgtatgtt ggaagccact ggtacgcaac
     1621 caccctgcag acactgctga agtatccaga actgctgtcc aaccctcaga gagtgtactg
     1681 gatcacatat ggacaaaccc tgctcatcca cggggatggc cagatgttcc gacacattct
     1741 caacttcctg agacttggca aactgttttt accatctgaa tttaaggaat ggcccctctt
     1801 ctgccaggag gtggaggaat accacattcc atccctctca gaagcccttg cacaatgtga
     1861 agcatacaag tcatggactc aggagaaaga atctgaaaat gaagaagctt tttccatcag
     1921 gaggctgcat gtggtgacag aagggccagg gtcactggtg gagttcagta gagacactaa
     1981 agaaaccaca gcctacatgc ctgtggactt cgaagactgc agtgacagga ctccatggaa
     2041 caaggctaag ggaaacctgg tcaggtccaa ccagatggat gaggctgagc agtacactcg
     2101 gcccatccag gtgtccctat gccgaaatgc caagagggct ggcaacccta gcacatactc
     2161 acactgccgt ggcttgtgta ccaatcctgg acactggggg agccaccctg agagcccccc
     2221 aaagaagaaa tgcaccacaa tcaacctcac acagaaatct gaaaccaaag accctcccgc
     2281 cactcccatg caaaaactca tctccctggt gagagaatgg gacatggtca attgcaaaca
     2341 gtgggaattc cagccactga cagccacacg gagcagcccc ttggaggagg ccaccctgca
     2401 gctccccttg ggaagcgagg ctgcttccca gcccagcacc tcagctgcct ggaaagccca
     2461 ttccacagcc tcagagaagg atccaggacc acaggcaggg gctggagctg gagcgaaaga
     2521 caaggggcca gagccaacct tcaagccata cttaccccca aaaagagctg gcaccctgaa
     2581 ggactggagc aagcagagga ccaaggagag agaaagccct gcccctgagc agcctctgcc
     2641 cgaggccagt gaggtggaca gcctaggggt tatcctcaaa gtgactcacc cccccgtggt
     2701 gggcagcgat ggcttctgca tgttctttga ggacagcatc atctatacca cggagatgga
     2761 caacctcagg cacacaacac ccacagccag tccccagccc caagaagtga ctttcctgag
     2821 tttctctctg tcctgggaag agatgtttta tgcacagaaa tgtcactgct tcctggctga
     2881 catcatcatg gattccatca ggcaaaagga ccccaaagcc atcacagcca aggtggtctc
     2941 cctggccaat cggctgtgga ccctgcacat cagccccaag cagtttgtgg tagatttgct
     3001 ggccatcacc ggcttcaagg atgaccggca cacccaggag cgcctgtaca gctgggtgga
     3061 gcttacactg cccttcgcca ggaaatatgg ccgatgcatg gacctgctca tccagagggg
     3121 cctgtctagg tctgtctctt actccatcct gggaaagtac ctacaagagg acttgCCAAC
     3181 TTTCTTGTAC AAAGTGGTAT ACCCATACGA TGTTCCAGAT TACGCTTAGT AATGAGAAAT
     3241 ACTCAGAAGA TGTCACTCAC CATAGTCCGC AGTGAAAGAT AAATGATCCC ATGTGACAAA
     3301 TTCCCATCGT TTTAGAGCTA GAAATAGCAA GTAGGCCACT GAGCAAGAAC TATGAATGAG
     3361 ATCTTTAGAC ATATTGCGGC CGCTTTCTCC GCGATCCAGA CATGATAAGA TACATTGATG
     3421 AGTTTGGACA AACCAAAACT AGAATGCAGT GAAAAAAATG CCTTATTTGT GAAATTTGTG
     3481 ATGCTATTGC CTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTATC TATAACAAGA
     3541 AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT
     3601 ATGAGTTAAA TCTTAAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT
     3661 CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC
     3721 AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG
     3781 AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAAGAA
     3841 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
     3901 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
     3961 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
     4021 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
     4081 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
     4141 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCATGTCTC CGGGAGCTGC
     4201 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACACGCGA GTTCGAAAGG GCCTCGTGAT
     4261 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC
     4321 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
     4381 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
     4441 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
     4501 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
     4561 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
     4621 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
     4681 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
     4741 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
     4801 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
     4861 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
     4921 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
     4981 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
     5041 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
     5101 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGTTC
     5161 ACGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
     5221 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
     5281 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
     5341 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT
     5401 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     5461 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     5521 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     5581 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT
     5641 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     5701 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     5761 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     5821 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC
     5881 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     5941 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     6001 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     6061 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
     6121 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC
     6181 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA
     6241 TGAGCGCCCA ATACGCAAAC CGCCTCTCAC CGCGCGTTGG CCGATTCATT AATGCAGCTG
     6301 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAAACC GCCTCTCCCC
     6361 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
     6421 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
     6481 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
     6541 AACAGCTATG ACCATGATTA CGCCAAGGTC GACTTAACCC TAGAAAGATA ATCATATTGT
     6601 GACGTACGTT AAAGATAATC ATGCGTAAAA TTGACGCATG TGTTTTATCG GTCTGTATAT
     6661 CGAGGTTTAT TTATTAATTT GAATAGATAT TAAGTTTTAT TATATTTACA CTTACATACT
     6721 AATAATAAAT TCAACAAACA ATTTATTTAT GTTTATTTAT TTATTAAAAA AAAACAAAAA
     6781 CTCAAAATTT CTTCTATAAA GTAACAAAAC TTTTAGCAGT GAAAAAAATG CTTTATTTGT
     6841 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
     6901 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
     6961 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTGGA GATTCATGCG
     7021 CCAGGTTTTC TGGTCATGCA CCACGTTCGT GGGCCCTCAG GAACCTCCAC GTCAGCGGTA
     7081 ACTGTGAAGC CCAGTCGCTC GTAAAAAGGA AGATTCCGGG GAGCACTGGT TTCGAGGAAC
     7141 GCCGGCACGC CAGCCCGTTC CGCGGCCTCA ACACCAGGAA GAACGACAGC GCTTCCCAAA
     7201 CCTTTACCTT GGTGATCTGG GCTGACACCG ACAGTGGCAA GAAACCACGC AGGCTCCTTT
     7261 GGTCTATGAG GAGCCAGCAA GCCCTCCATT TGCTGTTGTG CCGCCAACCT ACTCCCTGAC
     7321 AGCTCGGCCA TCCGCGGACC TATTTCCGCG AACACTGCGC CGGCTTCGAC GCTTTCCGGA
     7381 GTAGTCCAGA CCGCAACTGC GGCACCATCG TCCGCAACCC AAACTTTACC AATGTCCAGA
     7441 CCCACGCGCG TAAGAAACAG CTCTTGCAAC TCAGTTACCC TCTCTATGTG TCTGTCCGGA
     7501 TCTACGGTAT GGCGGGTCGC TGGATAATCT GCAAATGCTG CGGCAAGTGT TCGGACAGCC
     7561 CGTGGTACAT CGTCACGGGT AGCGAGCCTA ACAGTGGGCT TATATTCCGT CATGGGGCCG
     7621 GGGTTCTCCT CCACGTCGCC GGCCTGCTTC AGCAGGCTGA AGTTGGTGGC GCCGCTGCCC
     7681 CCGGGGAGCA TGTCAAGGTC AAAATCGTCA AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA
     7741 AAGTCGTCAA GGGCATCGGC TGGGAGCATG TCTAAGTCAA AATCGTCAAG GGCGTCGGTC
     7801 GGCCCGCCGC TTTCGCACTT TAGCTGTTTC TCCAGGCCAC ATATGATTAG TTCCAGGCCG
     7861 AAAAGGAAGG CAGGTTCGGC TCCCTGCCGG TCGAACAGCT CAATTGCTTG TTTCAGAAGT
     7921 GGGGGCATAG AATCGGTGGT AGGTGTCTCT CTTTCCTCTT TTGCTACTTG ATGCTCCTGT
     7981 TCCTCCAATA CGCAGCCCAG TGTAAAGTGG CCCACGGCGG ACAGAGCGTA CAGTGCGTTC
     8041 TCCAGGGAGA AGCCTTGCTG ACACAGGAAC GCGAGCTGAT TTTCCAGGGT TTCGTACTGT
     8101 TTCTCTGTTG GGCGGGTGCC GAGATGCACT TTAGCCCCGT CACGATGTGA GAGGAGAGCA
     8161 CAGCGGTATG ACTTGGCGTT GTTCCGCAGA AAGTCTTGCC ATGACTCGCC TTCCAGGGGG
     8221 CAGGAGTGGG TATGATGCCT GTCCAGCATC TCGATTGGCA GGGCATCGAG CAGGGCCCGC
     8281 TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC TGCTCAACTC CCAGCTTTTG AGCGAGTTTC
     8341 CTTGTCGTCA GGCCTTCGAT ACCGACTCCA TTGAGTAATT CCAGAGCAGA GTTTATGACT
     8401 TTGCTCTTGT CCAGTCTAGA CATCTTATCG TCATCGTCTT TGTAATCCAT GGTGGCGGAT
     8461 CCCGCGTCAC GACACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAAGAA CGTTCACGGC
     8521 GACTACTGCA CTTATATACG GTTCTCCCCC ACCCTCGGGA AAAAGGCGGA GCCAGTACAC
     8581 GACATCACTT TCCCAGTTTA CCCCGCGCCA CCTTCTCTAG GCACCGGTTC AATTGCCGAC
     8641 CCCTCCCCCC AACTTCTCGG GCACTGTGGG CCATGTGCGC TCTGCCCCAT GGTAATAGCC
     8701 ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTGCC ATAAGGTCAT GTACTGGGCA
     8761 TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA
     8821 TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA
     8881 TGGAAAGTGC CTATTGGCGT TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG
     8941 GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC
     9001 ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT
     9061 CAATGTCATA AGCTCTAATC GCATAATCGA GCTAAGAGCG ATTAATATGC TTAGCTAGCT
     9121 TAGCTAAGCT AGATTTCTCT ATTCTCCCAC AATATATAGC AGCTATTAGC TTAGCAGTCC
     9181 GAATACGATC GTCCACGATC CATAAGCGTA ACACATTTGA GTAGCGACTG ACGGGCACCG
     9241 GAGCCACTCG AGTGGAATTA CTGACGGGCA CCGGAGCCAC TCGAGTGGAA TT
//