LOCUS       CK530_37_TF103_BRCA1   10585 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..4462
                     /label="BRCA1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             398..4528
                     /label="Translation 398-4528"
                     /translation="MINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCPNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFALFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKGASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQRGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKDSHIHGQRDNSMFSKRPREHISVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEGLIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQGPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSIVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    4467..4488
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4467..4490)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(4470..4488)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(4474..4490)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(4474..4491)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 4474..4554
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4479..4488
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4493..4519
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4493..4519)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      4520..4528
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          4526..4554
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4529..4554)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     4532..4554
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     4532..4554
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          4532..4554
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 4555..4624
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 4559..4581
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4582..4601
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_difference complement(4602..4624)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4625..4628
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4629..4651)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4629..4651)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4629..4651)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 4629..4660
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           4652..4676
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 4661..4694
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4682..4703)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    4686..4820
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 4695..4850
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 4851..7561
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(5092..5126)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5138..5153)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5138..5153)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(5157..5173)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(5362..5381)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     5483..5505
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          5483..5505
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(5544..5562)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5618..5637
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        5630..5734
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5735..6595
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(5953..5972)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5953..5972)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      6766..7354
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     7255..7274
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     7508..7525
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 7562..7650
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 7651..10519
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    7731..7752
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        7767..7797
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    7805..7821
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          7812..7831
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     7829..7845
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   7871..7933
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    8109..8190
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     8217..8236
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          8224..8240
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          8271..8290
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(8283..8306)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(8307..8906)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     8792..8811
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          8907..8924
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(8907..8963)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(8973..9716)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(9114..9133)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          9317..9336
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     9359..9378
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(9413..9432)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(9717..9740)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(9740..9749)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(9769..9980)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(9981..10360)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     10509..10528
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 10520..10552
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 10553..10585
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(10562..10580)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg ataaatcagg gaactaacca
      421 aacggagcag aatggtcaag tgatgaatat tactaatagt ggtcatgaga ataaaacaaa
      481 aggtgattct attcagaatg agaaaaatcc taacccaata gaatcactcg aaaaagaatc
      541 tgctttcaaa acgaaagctg aacctataag cagcagtata agcaatatgg aactcgaatt
      601 aaatatccac aattcaaaag cacctaaaaa gaataggctg aggaggaagt cttctaccag
      661 gcatattcat gcgcttgaac tagtagtcag tagaaatcta agcccaccta attgtactga
      721 attgcaaatt gatagttgtt ctagcagtga agagataaag aaaaaaaagt acaaccaaat
      781 gccagtcagg cacagcagaa acctacaact catggaaggt aaagaacctg caactggagc
      841 caagaagagt aacaagccaa atgaacagac aagtaaaaga catgacagtg atactttccc
      901 agagctgaag ttaacaaatg cacctggttc ttttactaag tgtccaaata ccagtgaact
      961 taaagaattt gtcaatccta gccttccaag agaagaaaaa gaagagaaac tagaaacagt
     1021 taaagtgtct aataatgctg aagaccccaa agatctcatg ttaagtggag aaagggtttt
     1081 gcaaactgaa agatctgtag agagtagcag tatttcactg gtacctggta ctgattatgg
     1141 cactcaggaa agtatctcgt tactggaagt tagcactcta gggaaggcaa aaacagaacc
     1201 aaataaatgt gtgagtcagt gtgcagcatt tgaaaacccc aagggactaa ttcatggttg
     1261 ttccaaagat aatagaaatg acacagaagg ctttaagtat ccattgggac atgaagttaa
     1321 ccacagtcgg gaaacaagca tagaaatgga agaaagtgaa ctcgatgctc agtatttgca
     1381 gaatacattc aaggtttcaa agcgccagtc atttgctctg ttttcaaatc caggaaatgc
     1441 agaagaggaa tgtgcaacat tctctgccca ctctgggtcc ttaaagaaac aaagtccaaa
     1501 agtcactttt gaatgtgaac aaaaggaaga aaatcaagga aagaatgagt ctaatatcaa
     1561 gcctgtacag acagttaata tcactgcagg ctttcctgtg gttggtcaga aagataagcc
     1621 agttgataat gccaaatgta gtatcaaagg aggctctagg ttttgtctat catctcagtt
     1681 cagaggcaac gaaactggac tcattactcc aaataaacat ggacttttac aaaacccata
     1741 tcgtatacca ccactttttc ccatcaagtc atttgttaaa actaaatgta agaaaaatct
     1801 gctagaggaa aactttgagg aacattcaat gtcacctgaa agagaaatgg gaaatgagaa
     1861 cattccaagt acagtgagca caattagccg taataacatt agagaaaatg tttttaaagg
     1921 agccagctca agcaatatta atgaagtagg ttccagtact aatgaagtgg gctccagtat
     1981 taatgaaata ggttccagtg atgaaaacat tcaagcagaa ctaggtagaa acagagggcc
     2041 aaaattgaat gctatgctta gattaggggt tttgcaacct gaggtctata aacaaagtct
     2101 tcctggaagt aattgtaagc atcctgaaat aaaaaagcaa gaatatgaag aagtagttca
     2161 gactgttaat acagatttct ctccatatct gatttcagat aacttagaac agcctatggg
     2221 aagtagtcat gcatctcagg tttgttctga gacacctgat gacctgttag atgatggtga
     2281 aataaaggaa gatactagtt ttgctgaaaa tgacattaag gaaagttctg ctgtttttag
     2341 caaaagcgtc cagagaggag agcttagcag gagtcctagc cctttcaccc atacacattt
     2401 ggctcagggt taccgaagag gggccaagaa attagagtcc tcagaagaga acttatctag
     2461 tgaggatgaa gagcttccct gcttccaaca cttgttattt ggtaaagtaa acaatatacc
     2521 ttctcagtct actaggcata gcaccgttgc taccgagtgt ctgtctaaga acacagagga
     2581 gaatttatta tcattgaaga atagcttaaa tgactgcagt aaccaggtaa tattggcaaa
     2641 ggcatctcag gaacatcacc ttagtgagga aacaaaatgt tctgctagct tgttttcttc
     2701 acagtgcagt gaattggaag acttgactgc aaatacaaac acccaggatc ctttcttgat
     2761 tggttcttcc aaacaaatga ggcatcagtc tgaaagccag ggagttggtc tgagtgacaa
     2821 ggaattggtt tcagatgatg aagaaagagg aacgggcttg gaagaaaata atcaagaaga
     2881 gcaaagcatg gattcaaact taggtgaagc agcatctggg tgtgagagtg aaacaagcgt
     2941 ctctgaagac tgctcagggc tatcctctca gagtgacatt ttaaccactc agcagaggga
     3001 taccatgcaa cataacctga taaagctcca gcaggaaatg gctgaactag aagctgtgtt
     3061 agaacagcat gggagccagc cttctaacag ctacccttcc atcataagtg actcctctgc
     3121 ccttgaggac ctgcgaaatc cagaacaaag cacatcagaa aaagattcgc atatacatgg
     3181 ccaaagggac aactccatgt tttctaaaag gcctagagaa catatatcag tattaacttc
     3241 acagaaaagt agtgaatacc ctataagcca gaatccagaa ggcctttctg ctgacaagtt
     3301 tgaggtgtct gcagatagtt ctaccagtaa aaataaagaa ccaggagtgg aaaggtcatc
     3361 cccttctaaa tgcccatcat tagatgatag gtggtacatg cacagttgct ctgggagtct
     3421 tcagaataga aactacccat ctcaagaggg gctcattaag gttgttgatg tggaggagca
     3481 acagctggaa gagtctgggc cacacgattt gacggaaaca tcttacttgc caaggcaaga
     3541 tctagaggga accccttacc tggaatctgg aatcagcctc ttctctgatg accctgaatc
     3601 tgatccttct gaagacagag ccccagagtc agctcgtgtt ggcaacatac catcttcaac
     3661 ctctgcattg aaagttcccc aattgaaagt tgcagaatct gcccagggtc cagctgctgc
     3721 tcatactact gatactgctg ggtataatgc aatggaagaa agtgtgagca gggagaagcc
     3781 agaattgaca gcttcaacag aaagggtcaa caaaagaatg tccatagtgg tgtctggcct
     3841 gaccccagaa gaatttatgc tcgtgtacaa gtttgccaga aaacaccaca tcactttaac
     3901 taatctaatt actgaagaga ctactcatgt tgttatgaaa acagatgctg agtttgtgtg
     3961 tgaacggaca ctgaaatatt ttctaggaat tgcgggagga aaatgggtag ttagctattt
     4021 ctgggtgacc cagtctatta aagaaagaaa aatgctgaat gagcatgatt ttgaagtcag
     4081 aggagatgtg gtcaatggaa gaaaccacca aggtccaaag cgagcaagag aatcccagga
     4141 cagaaagatc ttcagggggc tagaaatctg ttgctatggg cccttcacca acatgcccac
     4201 agatcaactg gaatggatgg tacagctgtg tggtgcttct gtggtgaagg agctttcatc
     4261 attcaccctt ggcacaggtg tccacccaat tgtggttgtg cagccagatg cctggacaga
     4321 ggacaatggc ttccatgcaa ttgggcagat gtgtgaggca cctgtggtga cccgagagtg
     4381 ggtgttggac agtgtagcac tctaccagtg ccaggagctg gacacctacc tgatacccca
     4441 gatcccccac agccactact gaTTGGACCC AGCTTTCTTG TACAAAGTGG TATACCCATA
     4501 CGATGTTCCA GATTACGCTT AGTAATGAGA AATACTCAGA AGATGTCACT CACCATAGTC
     4561 CGCAGTGAAA GATAAATGAT CAGAAGACCT TGATAAGCGC TGTTTTAGAG CTAGAAATAG
     4621 CAAGTAGGCC ACTGAGCAAG AACTATGAAT GAGATCTTTA GACATATTGC GGCCGCTTTC
     4681 TCCGCGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCAAA ACTAGAATGC
     4741 AGTGAAAAAA ATGCCTTATT TGTGAAATTT GTGATGCTAT TGCCTTATTT GTAACCATTA
     4801 TAAGCTGCAA TAAACAAGTT ATCTATAACA AGAAAATATA TATATAATAA GTTATCACGT
     4861 AAGTAGAACA TGAAATAACA ATATAATTAT CGTATGAGTT AAATCTTAAA AGTCACGTAA
     4921 AAGATAATCA TGCGTCATTT TGACTCACGC GGTCGTTATA GTTCAAAATC AGTGACACTT
     4981 ACCGCATTGA CAAGCACGCC TCACGGGAGC TCCAAGCGGC GACTGAGATG TCCTAAATGC
     5041 ACAGCGACGG ATTCGCGCTA TTTAGAAAGA GAGAGCAATA TTTCAAGAAT GCATGCGTCA
     5101 ATTTTACGCA GACTATCTTT CTAGGGTTAA GAATTCACTG GCCGTCGTTT TACAACGTCG
     5161 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     5221 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
     5281 GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA
     5341 CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG
     5401 ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA
     5461 CAGACAAGCT GTGACCATGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA
     5521 CCGAAACACG CGAGTTCGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT
     5581 GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     5641 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
     5701 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC
     5761 CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT
     5821 GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT
     5881 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC
     5941 TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT
     6001 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA
     6061 GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA
     6121 TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT
     6181 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA
     6241 AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG
     6301 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT
     6361 GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT
     6421 TGCTGATAAA TCTGGAGCCG GTGAGCGTGG TTCACGCGGT ATCATTGCAG CACTGGGGCC
     6481 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA
     6541 TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC
     6601 AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG
     6661 GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC
     6721 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
     6781 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
     6841 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
     6901 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
     6961 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
     7021 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
     7081 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
     7141 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
     7201 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
     7261 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
     7321 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
     7381 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC
     7441 TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC
     7501 CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGATGAGCGC CCAATACGCA AACCGCCTCT
     7561 CACCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC
     7621 GGGCAGTGAG CGCAACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC
     7681 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT
     7741 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT
     7801 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG
     7861 GTCGACTTAA CCCTAGAAAG ATAATCATAT TGTGACGTAC GTTAAAGATA ATCATGCGTA
     7921 AAATTGACGC ATGTGTTTTA TCGGTCTGTA TATCGAGGTT TATTTATTAA TTTGAATAGA
     7981 TATTAAGTTT TATTATATTT ACACTTACAT ACTAATAATA AATTCAACAA ACAATTTATT
     8041 TATGTTTATT TATTTATTAA AAAAAAACAA AAACTCAAAA TTTCTTCTAT AAAGTAACAA
     8101 AACTTTTAGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT
     8161 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT
     8221 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
     8281 ATGGCTGATT ATGATCCTCT GGAGATTCAT GCGCCAGGTT TTCTGGTCAT GCACCACGTT
     8341 CGTGGGCCCT CAGGAACCTC CACGTCAGCG GTAACTGTGA AGCCCAGTCG CTCGTAAAAA
     8401 GGAAGATTCC GGGGAGCACT GGTTTCGAGG AACGCCGGCA CGCCAGCCCG TTCCGCGGCC
     8461 TCAACACCAG GAAGAACGAC AGCGCTTCCC AAACCTTTAC CTTGGTGATC TGGGCTGACA
     8521 CCGACAGTGG CAAGAAACCA CGCAGGCTCC TTTGGTCTAT GAGGAGCCAG CAAGCCCTCC
     8581 ATTTGCTGTT GTGCCGCCAA CCTACTCCCT GACAGCTCGG CCATCCGCGG ACCTATTTCC
     8641 GCGAACACTG CGCCGGCTTC GACGCTTTCC GGAGTAGTCC AGACCGCAAC TGCGGCACCA
     8701 TCGTCCGCAA CCCAAACTTT ACCAATGTCC AGACCCACGC GCGTAAGAAA CAGCTCTTGC
     8761 AACTCAGTTA CCCTCTCTAT GTGTCTGTCC GGATCTACGG TATGGCGGGT CGCTGGATAA
     8821 TCTGCAAATG CTGCGGCAAG TGTTCGGACA GCCCGTGGTA CATCGTCACG GGTAGCGAGC
     8881 CTAACAGTGG GCTTATATTC CGTCATGGGG CCGGGGTTCT CCTCCACGTC GCCGGCCTGC
     8941 TTCAGCAGGC TGAAGTTGGT GGCGCCGCTG CCCCCGGGGA GCATGTCAAG GTCAAAATCG
     9001 TCAAGAGCGT CAGCAGGCAG CATATCAAGG TCAAAGTCGT CAAGGGCATC GGCTGGGAGC
     9061 ATGTCTAAGT CAAAATCGTC AAGGGCGTCG GTCGGCCCGC CGCTTTCGCA CTTTAGCTGT
     9121 TTCTCCAGGC CACATATGAT TAGTTCCAGG CCGAAAAGGA AGGCAGGTTC GGCTCCCTGC
     9181 CGGTCGAACA GCTCAATTGC TTGTTTCAGA AGTGGGGGCA TAGAATCGGT GGTAGGTGTC
     9241 TCTCTTTCCT CTTTTGCTAC TTGATGCTCC TGTTCCTCCA ATACGCAGCC CAGTGTAAAG
     9301 TGGCCCACGG CGGACAGAGC GTACAGTGCG TTCTCCAGGG AGAAGCCTTG CTGACACAGG
     9361 AACGCGAGCT GATTTTCCAG GGTTTCGTAC TGTTTCTCTG TTGGGCGGGT GCCGAGATGC
     9421 ACTTTAGCCC CGTCACGATG TGAGAGGAGA GCACAGCGGT ATGACTTGGC GTTGTTCCGC
     9481 AGAAAGTCTT GCCATGACTC GCCTTCCAGG GGGCAGGAGT GGGTATGATG CCTGTCCAGC
     9541 ATCTCGATTG GCAGGGCATC GAGCAGGGCC CGCTTGTTCT TCACGTGCCA GTACAGGGTA
     9601 GGCTGCTCAA CTCCCAGCTT TTGAGCGAGT TTCCTTGTCG TCAGGCCTTC GATACCGACT
     9661 CCATTGAGTA ATTCCAGAGC AGAGTTTATG ACTTTGCTCT TGTCCAGTCT AGACATCTTA
     9721 TCGTCATCGT CTTTGTAATC CATGGTGGCG GATCCCGCGT CACGACACCT GTGTTCTGGC
     9781 GGCAAACCCG TTGCGAAAAA GAACGTTCAC GGCGACTACT GCACTTATAT ACGGTTCTCC
     9841 CCCACCCTCG GGAAAAAGGC GGAGCCAGTA CACGACATCA CTTTCCCAGT TTACCCCGCG
     9901 CCACCTTCTC TAGGCACCGG TTCAATTGCC GACCCCTCCC CCCAACTTCT CGGGCACTGT
     9961 GGGCCATGTG CGCTCTGCCC CATGGTAATA GCCATGACTA ATACGTAGAT GTACTGCCAA
    10021 GTAGGAAAGT GCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA
    10081 TTGACGTCAA TAGGGGGCGT ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA
    10141 GTTTACCGTA AATACTCCAC CCATTGACGT CAATGGAAAG TGCCTATTGG CGTTACTATG
    10201 GGAACATACG TCATTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG
    10261 CCATTTACCG TAAGTTATGT AACGCGGAAC TCCATATATG GGCTATGAAC TAATGACCCC
    10321 GTAATTGATT ACTATTAATA ACTAGTCAAT AATCAATGTC ATAAGCTCTA ATCGCATAAT
    10381 CGAGCTAAGA GCGATTAATA TGCTTAGCTA GCTTAGCTAA GCTAGATTTC TCTATTCTCC
    10441 CACAATATAT AGCAGCTATT AGCTTAGCAG TCCGAATACG ATCGTCCACG ATCCATAAGC
    10501 GTAACACATT TGAGTAGCGA CTGACGGGCA CCGGAGCCAC TCGAGTGGAA TTACTGACGG
    10561 GCACCGGAGC CACTCGAGTG GAATT
//