LOCUS       CK530_45_TF111_HOXA5    7333 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..1210
                     /label="HOXA5"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             398..1276
                     /label="Translation 398-1276"
                     /translation="MSSYFVNSFCGRYPNGPDYQLHNYGDHSSVSEQFRDSASMHSGRYGYGYNGMDLSVGRSGSGHFGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQSEPSPAPPAQPQIYPWMRKLHISHDNIGGPEGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSMAAAGGAFRP*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    1215..1236
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1215..1238)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1218..1236)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1222..1238)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1222..1239)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1222..1302
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1227..1236
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1241..1267
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1241..1267)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      1268..1276
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1274..1302
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1277..1302)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1280..1302
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1280..1302
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1280..1302
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 1303..1372
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1307..1329
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1330..1349
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_difference complement(1350..1372)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1373..1376
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1377..1399)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1377..1399)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1377..1399)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 1377..1408
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1400..1424
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1409..1442
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1430..1451)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    1434..1568
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1443..1598
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1599..4309
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(1840..1874)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(1886..1901)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1886..1901)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1905..1921)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2110..2129)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2231..2253
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2231..2253
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2292..2310)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2366..2385
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        2378..2482
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2483..3343
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2701..2720)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2701..2720)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3514..4102
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4003..4022
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4256..4273
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4310..4398
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4399..7267
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4479..4500
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4515..4545
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4553..4569
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4560..4579
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     4577..4593
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4619..4681
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4857..4938
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     4965..4984
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4972..4988
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5019..5038
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(5031..5054)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5055..5654)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5540..5559
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5655..5672
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5655..5711)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5721..6464)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(5862..5881)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          6065..6084
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     6107..6126
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(6161..6180)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(6465..6488)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6488..6497)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6517..6728)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(6729..7108)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7257..7276
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7268..7300
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 7301..7333
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7310..7328)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg agctcttatt ttgtaaactc
      421 attttgcggt cgctatccaa atggcccgga ctaccagttg cataattatg gagatcatag
      481 ttccgtgagc gagcaattca gggactcggc gagcatgcac tccggcaggt acggctacgg
      541 ctacaatggc atggatctca gcgtcggccg ctcgggctcc ggccactttg gctccggaga
      601 gcgcgcccgc agctacgctg ccagcgccag cgcggcgccc gccgagccca ggtacagcca
      661 gccggccacg tccacgcact ctcctcagcc cgatccgctg ccctgctccg ccgtggcccc
      721 ctcgcccggc agcgacagcc accacggcgg gaaaaactcc ctaagcaact ccagcggcgc
      781 ctcggccgac gccggcagca cccacatcag cagcagagag ggggttggca cggcgtccgg
      841 agccgaggag gacgcccctg ccagcagcga gcaggcgagt gcgcagagcg agccgagccc
      901 ggcgccgccc gcccaacccc agatctaccc ctggatgcgc aagctgcaca taagtcatga
      961 caacataggc ggcccggaag gcaaaagggc ccggacggcc tacacgcgct accagaccct
     1021 ggagctggag aaggagttcc acttcaaccg ttacctgacc cgcagaagga ggattgaaat
     1081 agcacatgct ctttgcctct ccgagagaca aattaaaatc tggttccaaa accggagaat
     1141 gaagtggaaa aaagataata agctgaaaag catgagcatg gccgcggcag gaggggcctt
     1201 ccgtccctga TTGGACCCAG CTTTCTTGTA CAAAGTGGTA TACCCATACG ATGTTCCAGA
     1261 TTACGCTTAG TAATGAGAAA TACTCAGAAG ATGTCACTCA CCATAGTCCG CAGTGAAAGA
     1321 TAAATGATCG ATGAGCGCTG CATTTTTACG TTTTAGAGCT AGAAATAGCA AGTAGGCCAC
     1381 TGAGCAAGAA CTATGAATGA GATCTTTAGA CATATTGCGG CCGCTTTCTC CGCGATCCAG
     1441 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT
     1501 GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA
     1561 AACAAGTTAT CTATAACAAG AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG
     1621 AAATAACAAT ATAATTATCG TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG
     1681 CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA
     1741 AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT
     1801 TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA
     1861 CTATCTTTCT AGGGTTAAGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     1921 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     1981 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     2041 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
     2101 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
     2161 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
     2221 GACCATGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACACGCG
     2281 AGTTCGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
     2341 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
     2401 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
     2461 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
     2521 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
     2581 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
     2641 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
     2701 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
     2761 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
     2821 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
     2881 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
     2941 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
     3001 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
     3061 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
     3121 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
     3181 TGGAGCCGGT GAGCGTGGTT CACGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
     3241 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
     3301 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
     3361 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
     3421 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
     3481 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     3541 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     3601 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     3661 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     3721 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     3781 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     3841 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     3901 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     3961 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     4021 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     4081 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
     4141 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
     4201 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
     4261 GTCAGTGAGC GAGGAAGCGG ATGAGCGCCC AATACGCAAA CCGCCTCTCA CCGCGCGTTG
     4321 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     4381 CAACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
     4441 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
     4501 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
     4561 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGGT CGACTTAACC
     4621 CTAGAAAGAT AATCATATTG TGACGTACGT TAAAGATAAT CATGCGTAAA ATTGACGCAT
     4681 GTGTTTTATC GGTCTGTATA TCGAGGTTTA TTTATTAATT TGAATAGATA TTAAGTTTTA
     4741 TTATATTTAC ACTTACATAC TAATAATAAA TTCAACAAAC AATTTATTTA TGTTTATTTA
     4801 TTTATTAAAA AAAAACAAAA ACTCAAAATT TCTTCTATAA AGTAACAAAA CTTTTAGCAG
     4861 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
     4921 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
     4981 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
     5041 GATCCTCTGG AGATTCATGC GCCAGGTTTT CTGGTCATGC ACCACGTTCG TGGGCCCTCA
     5101 GGAACCTCCA CGTCAGCGGT AACTGTGAAG CCCAGTCGCT CGTAAAAAGG AAGATTCCGG
     5161 GGAGCACTGG TTTCGAGGAA CGCCGGCACG CCAGCCCGTT CCGCGGCCTC AACACCAGGA
     5221 AGAACGACAG CGCTTCCCAA ACCTTTACCT TGGTGATCTG GGCTGACACC GACAGTGGCA
     5281 AGAAACCACG CAGGCTCCTT TGGTCTATGA GGAGCCAGCA AGCCCTCCAT TTGCTGTTGT
     5341 GCCGCCAACC TACTCCCTGA CAGCTCGGCC ATCCGCGGAC CTATTTCCGC GAACACTGCG
     5401 CCGGCTTCGA CGCTTTCCGG AGTAGTCCAG ACCGCAACTG CGGCACCATC GTCCGCAACC
     5461 CAAACTTTAC CAATGTCCAG ACCCACGCGC GTAAGAAACA GCTCTTGCAA CTCAGTTACC
     5521 CTCTCTATGT GTCTGTCCGG ATCTACGGTA TGGCGGGTCG CTGGATAATC TGCAAATGCT
     5581 GCGGCAAGTG TTCGGACAGC CCGTGGTACA TCGTCACGGG TAGCGAGCCT AACAGTGGGC
     5641 TTATATTCCG TCATGGGGCC GGGGTTCTCC TCCACGTCGC CGGCCTGCTT CAGCAGGCTG
     5701 AAGTTGGTGG CGCCGCTGCC CCCGGGGAGC ATGTCAAGGT CAAAATCGTC AAGAGCGTCA
     5761 GCAGGCAGCA TATCAAGGTC AAAGTCGTCA AGGGCATCGG CTGGGAGCAT GTCTAAGTCA
     5821 AAATCGTCAA GGGCGTCGGT CGGCCCGCCG CTTTCGCACT TTAGCTGTTT CTCCAGGCCA
     5881 CATATGATTA GTTCCAGGCC GAAAAGGAAG GCAGGTTCGG CTCCCTGCCG GTCGAACAGC
     5941 TCAATTGCTT GTTTCAGAAG TGGGGGCATA GAATCGGTGG TAGGTGTCTC TCTTTCCTCT
     6001 TTTGCTACTT GATGCTCCTG TTCCTCCAAT ACGCAGCCCA GTGTAAAGTG GCCCACGGCG
     6061 GACAGAGCGT ACAGTGCGTT CTCCAGGGAG AAGCCTTGCT GACACAGGAA CGCGAGCTGA
     6121 TTTTCCAGGG TTTCGTACTG TTTCTCTGTT GGGCGGGTGC CGAGATGCAC TTTAGCCCCG
     6181 TCACGATGTG AGAGGAGAGC ACAGCGGTAT GACTTGGCGT TGTTCCGCAG AAAGTCTTGC
     6241 CATGACTCGC CTTCCAGGGG GCAGGAGTGG GTATGATGCC TGTCCAGCAT CTCGATTGGC
     6301 AGGGCATCGA GCAGGGCCCG CTTGTTCTTC ACGTGCCAGT ACAGGGTAGG CTGCTCAACT
     6361 CCCAGCTTTT GAGCGAGTTT CCTTGTCGTC AGGCCTTCGA TACCGACTCC ATTGAGTAAT
     6421 TCCAGAGCAG AGTTTATGAC TTTGCTCTTG TCCAGTCTAG ACATCTTATC GTCATCGTCT
     6481 TTGTAATCCA TGGTGGCGGA TCCCGCGTCA CGACACCTGT GTTCTGGCGG CAAACCCGTT
     6541 GCGAAAAAGA ACGTTCACGG CGACTACTGC ACTTATATAC GGTTCTCCCC CACCCTCGGG
     6601 AAAAAGGCGG AGCCAGTACA CGACATCACT TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA
     6661 GGCACCGGTT CAATTGCCGA CCCCTCCCCC CAACTTCTCG GGCACTGTGG GCCATGTGCG
     6721 CTCTGCCCCA TGGTAATAGC CATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTGC
     6781 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
     6841 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
     6901 TACTCCACCC ATTGACGTCA ATGGAAAGTG CCTATTGGCG TTACTATGGG AACATACGTC
     6961 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
     7021 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC
     7081 TATTAATAAC TAGTCAATAA TCAATGTCAT AAGCTCTAAT CGCATAATCG AGCTAAGAGC
     7141 GATTAATATG CTTAGCTAGC TTAGCTAAGC TAGATTTCTC TATTCTCCCA CAATATATAG
     7201 CAGCTATTAG CTTAGCAGTC CGAATACGAT CGTCCACGAT CCATAAGCGT AACACATTTG
     7261 AGTAGCGACT GACGGGCACC GGAGCCACTC GAGTGGAATT ACTGACGGGC ACCGGAGCCA
     7321 CTCGAGTGGA ATT
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