LOCUS       CK530_49_TF115_TERF1    7767 bp ds-DNA     circular     09-OCT-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     misc_feature    1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..386
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..391
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    392..1650
                     /label="TERF1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             392..1711
                     /label="Translation 392-1711"
                     /translation="MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSEDCPTFLYKVVYPYDVPDYA***"
     misc_feature    1651..1671
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1653..1671)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1657..1673)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1657..1674)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1657..1737
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1662..1671
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1676..1702
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1676..1702)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      1703..1711
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1709..1737
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1712..1737)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     1715..1737
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1715..1737
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1715..1737
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1738..1806
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1742..1764
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1765..1783
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_difference complement(1784..1806)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1807..1810
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1811..1833)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1811..1833)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1811..1833)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1811..1842
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1834..1858
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1843..1876
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1864..1885)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    1868..2002
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1877..2032
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2033..4743
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2274..2308)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2320..2335)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2320..2335)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2339..2355)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2544..2563)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2665..2687
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2665..2687
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2726..2744)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2800..2819
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        2812..2916
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2917..3777
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3135..3154)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3135..3154)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3948..4536
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4437..4456
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4690..4707
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4744..4832
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4833..7701
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4913..4934
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4949..4979
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4987..5003
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4994..5013
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     5011..5027
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5053..5115
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5291..5372
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5399..5418
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5406..5422
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5453..5472
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(5465..5488)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5489..6088)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5974..5993
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6089..6106
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6089..6145)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6155..6898)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6296..6315)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          6499..6518
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     6541..6560
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(6595..6614)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(6899..6922)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(6922..6931)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6951..7162)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(7163..7542)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7691..7710
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7702..7734
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7735..7767
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7744..7762)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGttgg Catggcggag gatgtttcct cagcggcccc
      421 gagcccgcgg ggctgtgcgg atggtaggga tgccgaccct actgaggagc agatggcaga
      481 aacagagaga aacgacgagg agcagttcga atgccaggaa ctgctcgagt gccaggtgca
      541 ggtgggggcc cccgaggagg aggaggagga ggaggaggac gcgggcctgg tggccgaggc
      601 cgaggccgtg gctgccggct ggatgctcga tttcctctgc ctctctcttt gccgagcttt
      661 ccgcgacggc cgctccgagg acttccgcag gacccgcaac agcgcagagg ctattattca
      721 tggactatcc agtctaacag cttgccagtt gagaacgata tacatatgtc agtttttgac
      781 aagaattgca gcaggaaaaa cccttgatgc acagtttgaa aatgatgaac gaattacacc
      841 cttggaatca gccctgatga tttggggttc aattgaaaag gaacatgaca aacttcatga
      901 agaaatacag aatttaatta aaattcaggc tatagctgtt tgtatggaaa atggcaactt
      961 taaagaagca gaagaagtct ttgaaagaat atttggtgat ccaaattctc atatgccttt
     1021 caaaagcaaa ttgcttatga taatctctca gaaagataca tttcattcct tttttcaaca
     1081 cttcagctac aaccacatga tggagaaaat taagagttat gtgaattatg tgctaagtga
     1141 aaaatcatca acctttctaa tgaaggcagc ggcaaaagta gtagaaagca aaaggacaag
     1201 aacaataact tctcaagata aacctagtgg taatgatgtt gaaatggaaa ctgaagctaa
     1261 tttggataca agaaaaaggt ctcacaagaa tcttttctta tctaagttgc aacatggaac
     1321 ccagcaacaa gaccttaata agaaagaaag aagagtagga actcctcaaa gtacaaaaaa
     1381 gaaaaaagaa agcagaagag ccactgaaag cagaatacct gtttcaaaga gtcagccggt
     1441 aactcctgaa aaacatcgag ctagaaaaag acaggcatgg ctttgggaag aagacaagaa
     1501 tttgagatct ggcgtgagga aatatggaga gggaaactgg tctaaaatac tgttgcatta
     1561 taaattcaac aaccggacaa gtgtcatgtt aaaagacaga tggaggacca tgaagaaact
     1621 aaaactgatt tcctcagaca gcgaagactg CCCAaCTTTC TTGTACAAAG TGGTATACCC
     1681 ATACGATGTT CCAGATTACG CTTAGTAATG AGAAATACTC AGAAGATGTC ACTCACCATA
     1741 GTCCGCAGTG AAAGATAAAT GATCTGACGT TGGTTTGATA GCGGTTTTAG AGCTAGAAAT
     1801 AGCAAGTAGG CCACTGAGCA AGAACTATGA ATGAGATCTT TAGACATATT GCGGCCGCTT
     1861 TCTCCGCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA AAACTAGAAT
     1921 GCAGTGAAAA AAATGCCTTA TTTGTGAAAT TTGTGATGCT ATTGCCTTAT TTGTAACCAT
     1981 TATAAGCTGC AATAAACAAG TTATCTATAA CAAGAAAATA TATATATAAT AAGTTATCAC
     2041 GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG TTAAATCTTA AAAGTCACGT
     2101 AAAAGATAAT CATGCGTCAT TTTGACTCAC GCGGTCGTTA TAGTTCAAAA TCAGTGACAC
     2161 TTACCGCATT GACAAGCACG CCTCACGGGA GCTCCAAGCG GCGACTGAGA TGTCCTAAAT
     2221 GCACAGCGAC GGATTCGCGC TATTTAGAAA GAGAGAGCAA TATTTCAAGA ATGCATGCGT
     2281 CAATTTTACG CAGACTATCT TTCTAGGGTT AAGAATTCAC TGGCCGTCGT TTTACAACGT
     2341 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC
     2401 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC
     2461 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA
     2521 CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC
     2581 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT
     2641 TACAGACAAG CTGTGACCAT GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT
     2701 CACCGAAACA CGCGAGTTCG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC
     2761 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC
     2821 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC
     2881 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC
     2941 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG
     3001 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT
     3061 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC
     3121 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA
     3181 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA
     3241 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT
     3301 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT
     3361 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT
     3421 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG
     3481 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG
     3541 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT
     3601 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGTTCACGCG GTATCATTGC AGCACTGGGG
     3661 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG
     3721 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG
     3781 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA
     3841 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT
     3901 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT
     3961 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT
     4021 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG
     4081 ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA
     4141 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT
     4201 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG
     4261 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG
     4321 AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC
     4381 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA
     4441 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT
     4501 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA
     4561 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT
     4621 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG
     4681 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGATGAGC GCCCAATACG CAAACCGCCT
     4741 CTCACCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA
     4801 GCGGGCAGTG AGCGCAACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
     4861 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA
     4921 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT
     4981 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA
     5041 AGGTCGACTT AACCCTAGAA AGATAATCAT ATTGTGACGT ACGTTAAAGA TAATCATGCG
     5101 TAAAATTGAC GCATGTGTTT TATCGGTCTG TATATCGAGG TTTATTTATT AATTTGAATA
     5161 GATATTAAGT TTTATTATAT TTACACTTAC ATACTAATAA TAAATTCAAC AAACAATTTA
     5221 TTTATGTTTA TTTATTTATT AAAAAAAAAC AAAAACTCAA AATTTCTTCT ATAAAGTAAC
     5281 AAAACTTTTA GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
     5341 TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT
     5401 TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG
     5461 GTATGGCTGA TTATGATCCT CTGGAGATTC ATGCGCCAGG TTTTCTGGTC ATGCACCACG
     5521 TTCGTGGGCC CTCAGGAACC TCCACGTCAG CGGTAACTGT GAAGCCCAGT CGCTCGTAAA
     5581 AAGGAAGATT CCGGGGAGCA CTGGTTTCGA GGAACGCCGG CACGCCAGCC CGTTCCGCGG
     5641 CCTCAACACC AGGAAGAACG ACAGCGCTTC CCAAACCTTT ACCTTGGTGA TCTGGGCTGA
     5701 CACCGACAGT GGCAAGAAAC CACGCAGGCT CCTTTGGTCT ATGAGGAGCC AGCAAGCCCT
     5761 CCATTTGCTG TTGTGCCGCC AACCTACTCC CTGACAGCTC GGCCATCCGC GGACCTATTT
     5821 CCGCGAACAC TGCGCCGGCT TCGACGCTTT CCGGAGTAGT CCAGACCGCA ACTGCGGCAC
     5881 CATCGTCCGC AACCCAAACT TTACCAATGT CCAGACCCAC GCGCGTAAGA AACAGCTCTT
     5941 GCAACTCAGT TACCCTCTCT ATGTGTCTGT CCGGATCTAC GGTATGGCGG GTCGCTGGAT
     6001 AATCTGCAAA TGCTGCGGCA AGTGTTCGGA CAGCCCGTGG TACATCGTCA CGGGTAGCGA
     6061 GCCTAACAGT GGGCTTATAT TCCGTCATGG GGCCGGGGTT CTCCTCCACG TCGCCGGCCT
     6121 GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC TGCCCCCGGG GAGCATGTCA AGGTCAAAAT
     6181 CGTCAAGAGC GTCAGCAGGC AGCATATCAA GGTCAAAGTC GTCAAGGGCA TCGGCTGGGA
     6241 GCATGTCTAA GTCAAAATCG TCAAGGGCGT CGGTCGGCCC GCCGCTTTCG CACTTTAGCT
     6301 GTTTCTCCAG GCCACATATG ATTAGTTCCA GGCCGAAAAG GAAGGCAGGT TCGGCTCCCT
     6361 GCCGGTCGAA CAGCTCAATT GCTTGTTTCA GAAGTGGGGG CATAGAATCG GTGGTAGGTG
     6421 TCTCTCTTTC CTCTTTTGCT ACTTGATGCT CCTGTTCCTC CAATACGCAG CCCAGTGTAA
     6481 AGTGGCCCAC GGCGGACAGA GCGTACAGTG CGTTCTCCAG GGAGAAGCCT TGCTGACACA
     6541 GGAACGCGAG CTGATTTTCC AGGGTTTCGT ACTGTTTCTC TGTTGGGCGG GTGCCGAGAT
     6601 GCACTTTAGC CCCGTCACGA TGTGAGAGGA GAGCACAGCG GTATGACTTG GCGTTGTTCC
     6661 GCAGAAAGTC TTGCCATGAC TCGCCTTCCA GGGGGCAGGA GTGGGTATGA TGCCTGTCCA
     6721 GCATCTCGAT TGGCAGGGCA TCGAGCAGGG CCCGCTTGTT CTTCACGTGC CAGTACAGGG
     6781 TAGGCTGCTC AACTCCCAGC TTTTGAGCGA GTTTCCTTGT CGTCAGGCCT TCGATACCGA
     6841 CTCCATTGAG TAATTCCAGA GCAGAGTTTA TGACTTTGCT CTTGTCCAGT CTAGACATCT
     6901 TATCGTCATC GTCTTTGTAA TCCATGGTGG CGGATCCCGC GTCACGACAC CTGTGTTCTG
     6961 GCGGCAAACC CGTTGCGAAA AAGAACGTTC ACGGCGACTA CTGCACTTAT ATACGGTTCT
     7021 CCCCCACCCT CGGGAAAAAG GCGGAGCCAG TACACGACAT CACTTTCCCA GTTTACCCCG
     7081 CGCCACCTTC TCTAGGCACC GGTTCAATTG CCGACCCCTC CCCCCAACTT CTCGGGCACT
     7141 GTGGGCCATG TGCGCTCTGC CCCATGGTAA TAGCCATGAC TAATACGTAG ATGTACTGCC
     7201 AAGTAGGAAA GTGCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT
     7261 CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG
     7321 CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTGCCTATT GGCGTTACTA
     7381 TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG
     7441 GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC
     7501 CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG TCATAAGCTC TAATCGCATA
     7561 ATCGAGCTAA GAGCGATTAA TATGCTTAGC TAGCTTAGCT AAGCTAGATT TCTCTATTCT
     7621 CCCACAATAT ATAGCAGCTA TTAGCTTAGC AGTCCGAATA CGATCGTCCA CGATCCATAA
     7681 GCGTAACACA TTTGAGTAGC GACTGACGGG CACCGGAGCC ACTCGAGTGG AATTACTGAC
     7741 GGGCACCGGA GCCACTCGAG TGGAATT
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