LOCUS       CK530_51_TF117_RBM46    8122 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..1999
                     /label="RBM46"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             398..2065
                     /label="Translation 398-2065"
                     /translation="MNEENIDGTNGCSKVRTGIQNEAALLALMEKTGYNMVQENGQRKFGGPPPGWEGPPPPRGCEVFVGKIPRDMYEDELVPVFERAGKIYEFRLMMEFSGENRGYAFVMYTTKEEAQLAIRILNNYEIRPGKFIGVCVSLDNCRLFIGAIPKEKKKEEILDEMKKVTEGVVDVIVYPSATDKTKNRGFAFVEYESHRAAAMARRKLIPGTFQLWGHTIQVDWADPEKEVDEETMQRVKVLYVRNLMISTTEETIKAEFNKFKPGAVERVKKLRDYAFVHFFNREDAVAAMSVMNGKCIDGASIEVTLAKPVNKENTWRQHLNGQISPNSENLIVFANKEESHPKTLGKLPTLPARLNGQHSPSPPEVERCTYPFYPGTKLTPISMYSLKSNHFNSAVMHLDYYCNKNNWAPPEYYLYSTTSQDGKVLLVYKIVIPAIANGSQSYFMPDKLCTTLEDAKELAAQFTLLHLDYNFHRSSINSLSPVSATLSSGTPSVLPYTSRPYSYPGYPLSPTISLANGSHVGQRLCISNQASFF*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    2004..2025
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2004..2027)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2007..2025)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2011..2027)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2011..2028)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2011..2091
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2016..2025
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2030..2056
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2030..2056)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2057..2065
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2063..2091
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2066..2091)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2069..2091
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     2069..2091
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2069..2091
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2092..2161
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2096..2118
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2119..2138
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_difference complement(2139..2161)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2162..2165
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2166..2188)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2166..2188)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2166..2188)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2166..2197
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2189..2213
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2198..2231
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2219..2240)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2223..2357
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2232..2387
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2388..5098
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2629..2663)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2675..2690)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2675..2690)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2694..2710)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2899..2918)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3020..3042
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3020..3042
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3081..3099)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3155..3174
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3167..3271
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3272..4132
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3490..3509)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3490..3509)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4303..4891
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4792..4811
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5045..5062
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5099..5187
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5188..8056
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5268..5289
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5304..5334
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5342..5358
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5349..5368
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     5366..5382
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5408..5470
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5646..5727
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5754..5773
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5761..5777
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5808..5827
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(5820..5843)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5844..6443)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6329..6348
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6444..6461
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6444..6500)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6510..7253)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6651..6670)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          6854..6873
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     6896..6915
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(6950..6969)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7254..7277)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7277..7286)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7306..7517)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7518..7897)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8046..8065
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8057..8089
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8090..8122
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8099..8117)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg aatgaagaaa atatagatgg
      421 aacaaatgga tgcagtaaag ttcgaactgg tattcagaat gaagcagcat tacttgcttt
      481 gatggaaaag actggttaca acatggttca ggaaaatgga caaaggaaat ttggcggtcc
      541 tcctccaggt tgggaaggtc cacctccacc tagaggctgt gaagtttttg taggaaaaat
      601 acctcgtgat atgtatgaag atgagttagt tcctgtattt gaaagagctg ggaagatata
      661 tgaatttcga cttatgatgg aatttagtgg tgaaaatcga ggttatgctt ttgtgatgta
      721 cactacaaaa gaagaagccc aattagccat cagaattctt aataattatg aaattcgacc
      781 agggaagttt attggtgtgt gtgtaagcct ggataattgt agattattta ttggagctat
      841 tcccaaggaa aagaagaaag aagaaatttt agatgaaatg aagaaagtta cagaaggagt
      901 tgtagatgtc attgtttatc caagtgcaac tgataagacc aaaaatcgtg gttttgcatt
      961 tgtggaatat gaatctcaca gagctgctgc tatggcaagg aggaaactaa ttccaggaac
     1021 attccaacta tggggccaca ccattcaggt agattgggct gacccagaga aagaggtgga
     1081 tgaggaaacc atgcagagag ttaaagttct ttatgtaaga aatttaatga tctcaactac
     1141 agaggaaaca attaaagcag aattcaataa atttaagcct ggtgcagttg aacgggtaaa
     1201 gaaacttaga gattatgctt ttgttcactt tttcaaccga gaagatgcag tggctgccat
     1261 gtctgttatg aatggaaaat gcattgatgg agcaagtatt gaggtaacac tagctaaacc
     1321 agtaaataaa gaaaacactt ggagacagca tcttaatggt cagattagtc caaattctga
     1381 aaatctgatt gtgtttgcta acaaagaaga gagccaccca aaaactctag gcaagctgcc
     1441 aactcttcct gctcgtctca atggtcagca tagcccaagt ccgcctgaag ttgaaagatg
     1501 cacttaccct ttttatcctg gaacaaagct tactccaatt agtatgtatt ctttaaaatc
     1561 caatcatttt aattctgcag taatgcattt ggattattac tgcaacaaaa ataactgggc
     1621 accaccagaa tattatttat attcaacaac aagtcaagat gggaaagtac tcttggtgta
     1681 taagatagtt attcctgcta ttgcaaatgg atcccagagt tacttcatgc cagacaaact
     1741 ctgtactacg ttagaagatg caaaggaact ggcagcccag tttacattac ttcatttgga
     1801 ctacaatttc catcgcagct caataaatag tctttcccct gttagtgcta ccctctcttc
     1861 tgggactccc agcgtgcttc cttatacttc aaggccttat tcttatccag gctatccttt
     1921 gtcaccaaca atatcacttg ctaatggcag ccatgttgga cagcggctat gtatctccaa
     1981 tcaggcctcc ttcttctgaT TGGACCCAGC TTTCTTGTAC AAAGTGGTAT ACCCATACGA
     2041 TGTTCCAGAT TACGCTTAGT AATGAGAAAT ACTCAGAAGA TGTCACTCAC CATAGTCCGC
     2101 AGTGAAAGAT AAATGATCTG ACTAAGTATT CCATTGGCGT TTTAGAGCTA GAAATAGCAA
     2161 GTAGGCCACT GAGCAAGAAC TATGAATGAG ATCTTTAGAC ATATTGCGGC CGCTTTCTCC
     2221 GCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCAAAACT AGAATGCAGT
     2281 GAAAAAAATG CCTTATTTGT GAAATTTGTG ATGCTATTGC CTTATTTGTA ACCATTATAA
     2341 GCTGCAATAA ACAAGTTATC TATAACAAGA AAATATATAT ATAATAAGTT ATCACGTAAG
     2401 TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAA TCTTAAAAGT CACGTAAAAG
     2461 ATAATCATGC GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC
     2521 GCATTGACAA GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA
     2581 GCGACGGATT CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT
     2641 TTACGCAGAC TATCTTTCTA GGGTTAAGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA
     2701 CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG
     2761 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA
     2821 TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
     2881 CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA
     2941 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG
     3001 ACAAGCTGTG ACCATGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
     3061 AAACACGCGA GTTCGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT
     3121 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT
     3181 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA
     3241 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT
     3301 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA
     3361 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA
     3421 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT
     3481 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG
     3541 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA
     3601 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA
     3661 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT
     3721 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC
     3781 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA
     3841 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA
     3901 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC
     3961 TGATAAATCT GGAGCCGGTG AGCGTGGTTC ACGCGGTATC ATTGCAGCAC TGGGGCCAGA
     4021 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA
     4081 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA
     4141 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT
     4201 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT
     4261 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
     4321 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
     4381 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
     4441 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC
     4501 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
     4561 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
     4621 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
     4681 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
     4741 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
     4801 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
     4861 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
     4921 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
     4981 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA
     5041 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA TGAGCGCCCA ATACGCAAAC CGCCTCTCAC
     5101 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG
     5161 CAGTGAGCGC AACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
     5221 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG
     5281 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA
     5341 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGGTC
     5401 GACTTAACCC TAGAAAGATA ATCATATTGT GACGTACGTT AAAGATAATC ATGCGTAAAA
     5461 TTGACGCATG TGTTTTATCG GTCTGTATAT CGAGGTTTAT TTATTAATTT GAATAGATAT
     5521 TAAGTTTTAT TATATTTACA CTTACATACT AATAATAAAT TCAACAAACA ATTTATTTAT
     5581 GTTTATTTAT TTATTAAAAA AAAACAAAAA CTCAAAATTT CTTCTATAAA GTAACAAAAC
     5641 TTTTAGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA
     5701 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG
     5761 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
     5821 GCTGATTATG ATCCTCTGGA GATTCATGCG CCAGGTTTTC TGGTCATGCA CCACGTTCGT
     5881 GGGCCCTCAG GAACCTCCAC GTCAGCGGTA ACTGTGAAGC CCAGTCGCTC GTAAAAAGGA
     5941 AGATTCCGGG GAGCACTGGT TTCGAGGAAC GCCGGCACGC CAGCCCGTTC CGCGGCCTCA
     6001 ACACCAGGAA GAACGACAGC GCTTCCCAAA CCTTTACCTT GGTGATCTGG GCTGACACCG
     6061 ACAGTGGCAA GAAACCACGC AGGCTCCTTT GGTCTATGAG GAGCCAGCAA GCCCTCCATT
     6121 TGCTGTTGTG CCGCCAACCT ACTCCCTGAC AGCTCGGCCA TCCGCGGACC TATTTCCGCG
     6181 AACACTGCGC CGGCTTCGAC GCTTTCCGGA GTAGTCCAGA CCGCAACTGC GGCACCATCG
     6241 TCCGCAACCC AAACTTTACC AATGTCCAGA CCCACGCGCG TAAGAAACAG CTCTTGCAAC
     6301 TCAGTTACCC TCTCTATGTG TCTGTCCGGA TCTACGGTAT GGCGGGTCGC TGGATAATCT
     6361 GCAAATGCTG CGGCAAGTGT TCGGACAGCC CGTGGTACAT CGTCACGGGT AGCGAGCCTA
     6421 ACAGTGGGCT TATATTCCGT CATGGGGCCG GGGTTCTCCT CCACGTCGCC GGCCTGCTTC
     6481 AGCAGGCTGA AGTTGGTGGC GCCGCTGCCC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA
     6541 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG
     6601 TCTAAGTCAA AATCGTCAAG GGCGTCGGTC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC
     6661 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGCCGG
     6721 TCGAACAGCT CAATTGCTTG TTTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT
     6781 CTTTCCTCTT TTGCTACTTG ATGCTCCTGT TCCTCCAATA CGCAGCCCAG TGTAAAGTGG
     6841 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC
     6901 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT
     6961 TTAGCCCCGT CACGATGTGA GAGGAGAGCA CAGCGGTATG ACTTGGCGTT GTTCCGCAGA
     7021 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGGAGTGGG TATGATGCCT GTCCAGCATC
     7081 TCGATTGGCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC
     7141 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTCCA
     7201 TTGAGTAATT CCAGAGCAGA GTTTATGACT TTGCTCTTGT CCAGTCTAGA CATCTTATCG
     7261 TCATCGTCTT TGTAATCCAT GGTGGCGGAT CCCGCGTCAC GACACCTGTG TTCTGGCGGC
     7321 AAACCCGTTG CGAAAAAGAA CGTTCACGGC GACTACTGCA CTTATATACG GTTCTCCCCC
     7381 ACCCTCGGGA AAAAGGCGGA GCCAGTACAC GACATCACTT TCCCAGTTTA CCCCGCGCCA
     7441 CCTTCTCTAG GCACCGGTTC AATTGCCGAC CCCTCCCCCC AACTTCTCGG GCACTGTGGG
     7501 CCATGTGCGC TCTGCCCCAT GGTAATAGCC ATGACTAATA CGTAGATGTA CTGCCAAGTA
     7561 GGAAAGTGCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG
     7621 ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT
     7681 TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTGC CTATTGGCGT TACTATGGGA
     7741 ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA
     7801 TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA TGACCCCGTA
     7861 ATTGATTACT ATTAATAACT AGTCAATAAT CAATGTCATA AGCTCTAATC GCATAATCGA
     7921 GCTAAGAGCG ATTAATATGC TTAGCTAGCT TAGCTAAGCT AGATTTCTCT ATTCTCCCAC
     7981 AATATATAGC AGCTATTAGC TTAGCAGTCC GAATACGATC GTCCACGATC CATAAGCGTA
     8041 ACACATTTGA GTAGCGACTG ACGGGCACCG GAGCCACTCG AGTGGAATTA CTGACGGGCA
     8101 CCGGAGCCAC TCGAGTGGAA TT
//