LOCUS       CK530_52_TF118_RFX5     8371 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..2248
                     /label="RFX5"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             398..2314
                     /label="Translation 398-2314"
                     /translation="MAEDEPDAKSPKTGGRAPPGGAEAGEPTTLLQRLRGTISKAVQNKVEGILQDVQKFSDNDKLYLYLQLPSGPTTGDKSSEPSTLSNEEYMYAYRWIRNHLEEHTDTCLPKQSVYDAYRKYCESLACCRPLSTANFGKIIREIFPDIKARRLGGRGQSKYCYSGIRRKTLVSMPPLPGLDLKGSESPEMGPEVTPAPRDELVEAACALTCDWAERILKRSFSSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLAQPPKDLEARTGAGPLARGERKKSVVESSAPGANNLQVNALVARLPLLLPRAPRSLIPPIPVSPPILAPRLSSGALKVATLPLSSRAGAPPAAVPIINMILPTVPALPGPGPGPGRAPPGGLTQPRGTENREVGIGGDQGPHDKGVKRTAEVPVSEASGQAPPAKAAKQDIEDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAMESAQSSRLPWETWGSGGEGNSAGGAERPGPMGEAEKGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPLVPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDLKEHVLQSSLSQEHKDPKATPP*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    2253..2274
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2253..2276)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2256..2274)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2260..2276)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2260..2277)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2260..2340
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2265..2274
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2279..2305
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2279..2305)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2306..2314
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2312..2340
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2315..2340)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2318..2340
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     2318..2340
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          2318..2340
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2341..2410
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2345..2367
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2368..2387
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference complement(2388..2410)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2411..2414
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2415..2437)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2415..2437)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2415..2437)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2415..2446
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2438..2462
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2447..2480
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2468..2489)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2472..2606
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2481..2636
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2637..5347
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2878..2912)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2924..2939)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2924..2939)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2943..2959)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3148..3167)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3269..3291
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3269..3291
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3330..3348)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3404..3423
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3416..3520
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3521..4381
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3739..3758)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3739..3758)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4552..5140
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5041..5060
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5294..5311
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5348..5436
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5437..8305
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5517..5538
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5553..5583
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5591..5607
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5598..5617
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     5615..5631
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5657..5719
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5895..5976
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6003..6022
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6010..6026
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6057..6076
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6069..6092)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6093..6692)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6578..6597
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6693..6710
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6693..6749)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6759..7502)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6900..6919)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7103..7122
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     7145..7164
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7199..7218)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7503..7526)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7526..7535)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7555..7766)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7767..8146)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8295..8314
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8306..8338
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8339..8371
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8348..8366)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg gcagaagatg agcctgatgc
      421 taagagcccc aagactgggg gaagggcccc cccaggtggt gctgaggctg gggaacctac
      481 cacccttctt cagaggctcc gaggtaccat ttccaaggcc gtgcagaaca aagtagaggg
      541 gatcctgcaa gatgtacaga aattttctga caatgacaag ctgtatctct accttcagct
      601 cccctcagga cccaccactg gagacaaaag ctcagagcca agtacactga gcaatgagga
      661 gtacatgtat gcctataggt ggatccgcaa ccacctggaa gagcacactg acacctgtct
      721 gccaaagcaa agtgtttatg atgcctatcg gaagtactgt gagagtcttg cctgttgccg
      781 cccactcagc acagccaact ttggcaagat catcagagag atcttccctg acatcaaagc
      841 tcgaaggctt ggtggccggg gccagtccaa atattgctac agtggcataa ggaggaagac
      901 cttggtgtct atgccacccc tgcctggact tgacctaaag ggttctgaga gtccagaaat
      961 gggcccagaa gtaaccccag cacctcgaga tgaactggtg gaggcagcgt gtgccctgac
     1021 ctgtgactgg gcagagcgga tcctgaaacg gtccttcagt tccatcgttg aggtcgcccg
     1081 cttcctgcta cagcagcatc tcatctctgc ccgatctgca catgcccatg tgcttaaggc
     1141 catggggctt gctgaagagg acgaacatgc acctcgggaa cggtcatcta aaccaaagaa
     1201 tggtttagag aacccagagg gtggagccca caagaagcca gagagactgg cccagcctcc
     1261 taaggatctg gaagcccgaa ctggggccgg tcctctcgca cgtggagagc ggaagaagag
     1321 tgtagttgag agctcggccc caggagccaa taacctgcag gttaatgccc tagtggctcg
     1381 gctgcctctg ctccttcccc gggcccctcg ctcactaatt ccgccaatcc cagtctctcc
     1441 acctattctg gcccccaggc tttcttcagg tgccctgaaa gtggctacac tgcctctgtc
     1501 tagtagggcc ggggcacccc cagcagctgt gcccatcatt aacatgatct taccaactgt
     1561 tcctgctttg cctggacctg gacctgggcc tgggcgagct ccacctgggg gactcactca
     1621 gccccggggc acagagaaca gagaggtagg cataggtggt gaccaaggac cacatgacaa
     1681 gggtgtcaag aggacagctg aagtacctgt gagtgaggcc agtgggcagg ctccaccagc
     1741 taaagcagca aagcaggata tagaggatac agcaagtgat gccaaaagga aacgggggcg
     1801 ccctcgaaaa aagtcaggtg gaagtgggga aaggaattct acccctctca agtcagcagc
     1861 tgccatggaa tctgcccagt cctcaaggtt accatgggag acatggggct caggagggga
     1921 aggcaactca gctggagggg cagagaggcc agggccaatg ggagaggctg aaaagggggc
     1981 agtacttgcc cagggtcagg gagatggtac tgtttccaaa ggaggaaggg gccccggttc
     2041 ccagcatacc aaagaagcag aagataaaat tcccttggtc ccctcaaaag tgagtgtcat
     2101 caagggcagc agaagccaaa aggaggcttt tcctttggca aagggagagg tagacactgc
     2161 accacagggt aataaagact taaaggagca tgtgcttcaa agttccttat cccaggagca
     2221 taaagaccca aaagcaacac ccccatgaTT GGACCCAGCT TTCTTGTACA AAGTGGTATA
     2281 CCCATACGAT GTTCCAGATT ACGCTTAGTA ATGAGAAATA CTCAGAAGAT GTCACTCACC
     2341 ATAGTCCGCA GTGAAAGATA AATGATCAAT GACTTTGAGC CAACAGAGTT TTAGAGCTAG
     2401 AAATAGCAAG TAGGCCACTG AGCAAGAACT ATGAATGAGA TCTTTAGACA TATTGCGGCC
     2461 GCTTTCTCCG CGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCAAAACTA
     2521 GAATGCAGTG AAAAAAATGC CTTATTTGTG AAATTTGTGA TGCTATTGCC TTATTTGTAA
     2581 CCATTATAAG CTGCAATAAA CAAGTTATCT ATAACAAGAA AATATATATA TAATAAGTTA
     2641 TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC
     2701 ACGTAAAAGA TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG
     2761 ACACTTACCG CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT
     2821 AAATGCACAG CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT
     2881 GCGTCAATTT TACGCAGACT ATCTTTCTAG GGTTAAGAAT TCACTGGCCG TCGTTTTACA
     2941 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC
     3001 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG
     3061 CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT
     3121 TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA
     3181 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC
     3241 CGCTTACAGA CAAGCTGTGA CCATGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG
     3301 TCATCACCGA AACACGCGAG TTCGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
     3361 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     3421 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     3481 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     3541 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     3601 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     3661 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     3721 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     3781 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     3841 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT
     3901 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
     3961 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT
     4021 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC
     4081 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
     4141 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
     4201 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCA CGCGGTATCA TTGCAGCACT
     4261 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
     4321 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA
     4381 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     4441 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     4501 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     4561 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     4621 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     4681 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     4741 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     4801 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     4861 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     4921 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     4981 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     5041 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     5101 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     5161 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     5221 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG
     5281 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAT GAGCGCCCAA TACGCAAACC
     5341 GCCTCTCACC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG
     5401 GAAAGCGGGC AGTGAGCGCA ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     5461 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     5521 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
     5581 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
     5641 GCCAAGGTCG ACTTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA
     5701 TGCGTAAAAT TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG
     5761 AATAGATATT AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA
     5821 TTTATTTATG TTTATTTATT TATTAAAAAA AAACAAAAAC TCAAAATTTC TTCTATAAAG
     5881 TAACAAAACT TTTAGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT
     5941 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT
     6001 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA
     6061 TGTGGTATGG CTGATTATGA TCCTCTGGAG ATTCATGCGC CAGGTTTTCT GGTCATGCAC
     6121 CACGTTCGTG GGCCCTCAGG AACCTCCACG TCAGCGGTAA CTGTGAAGCC CAGTCGCTCG
     6181 TAAAAAGGAA GATTCCGGGG AGCACTGGTT TCGAGGAACG CCGGCACGCC AGCCCGTTCC
     6241 GCGGCCTCAA CACCAGGAAG AACGACAGCG CTTCCCAAAC CTTTACCTTG GTGATCTGGG
     6301 CTGACACCGA CAGTGGCAAG AAACCACGCA GGCTCCTTTG GTCTATGAGG AGCCAGCAAG
     6361 CCCTCCATTT GCTGTTGTGC CGCCAACCTA CTCCCTGACA GCTCGGCCAT CCGCGGACCT
     6421 ATTTCCGCGA ACACTGCGCC GGCTTCGACG CTTTCCGGAG TAGTCCAGAC CGCAACTGCG
     6481 GCACCATCGT CCGCAACCCA AACTTTACCA ATGTCCAGAC CCACGCGCGT AAGAAACAGC
     6541 TCTTGCAACT CAGTTACCCT CTCTATGTGT CTGTCCGGAT CTACGGTATG GCGGGTCGCT
     6601 GGATAATCTG CAAATGCTGC GGCAAGTGTT CGGACAGCCC GTGGTACATC GTCACGGGTA
     6661 GCGAGCCTAA CAGTGGGCTT ATATTCCGTC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG
     6721 GCCTGCTTCA GCAGGCTGAA GTTGGTGGCG CCGCTGCCCC CGGGGAGCAT GTCAAGGTCA
     6781 AAATCGTCAA GAGCGTCAGC AGGCAGCATA TCAAGGTCAA AGTCGTCAAG GGCATCGGCT
     6841 GGGAGCATGT CTAAGTCAAA ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT TTCGCACTTT
     6901 AGCTGTTTCT CCAGGCCACA TATGATTAGT TCCAGGCCGA AAAGGAAGGC AGGTTCGGCT
     6961 CCCTGCCGGT CGAACAGCTC AATTGCTTGT TTCAGAAGTG GGGGCATAGA ATCGGTGGTA
     7021 GGTGTCTCTC TTTCCTCTTT TGCTACTTGA TGCTCCTGTT CCTCCAATAC GCAGCCCAGT
     7081 GTAAAGTGGC CCACGGCGGA CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA GCCTTGCTGA
     7141 CACAGGAACG CGAGCTGATT TTCCAGGGTT TCGTACTGTT TCTCTGTTGG GCGGGTGCCG
     7201 AGATGCACTT TAGCCCCGTC ACGATGTGAG AGGAGAGCAC AGCGGTATGA CTTGGCGTTG
     7261 TTCCGCAGAA AGTCTTGCCA TGACTCGCCT TCCAGGGGGC AGGAGTGGGT ATGATGCCTG
     7321 TCCAGCATCT CGATTGGCAG GGCATCGAGC AGGGCCCGCT TGTTCTTCAC GTGCCAGTAC
     7381 AGGGTAGGCT GCTCAACTCC CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG GCCTTCGATA
     7441 CCGACTCCAT TGAGTAATTC CAGAGCAGAG TTTATGACTT TGCTCTTGTC CAGTCTAGAC
     7501 ATCTTATCGT CATCGTCTTT GTAATCCATG GTGGCGGATC CCGCGTCACG ACACCTGTGT
     7561 TCTGGCGGCA AACCCGTTGC GAAAAAGAAC GTTCACGGCG ACTACTGCAC TTATATACGG
     7621 TTCTCCCCCA CCCTCGGGAA AAAGGCGGAG CCAGTACACG ACATCACTTT CCCAGTTTAC
     7681 CCCGCGCCAC CTTCTCTAGG CACCGGTTCA ATTGCCGACC CCTCCCCCCA ACTTCTCGGG
     7741 CACTGTGGGC CATGTGCGCT CTGCCCCATG GTAATAGCCA TGACTAATAC GTAGATGTAC
     7801 TGCCAAGTAG GAAAGTGCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
     7861 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
     7921 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTGCC TATTGGCGTT
     7981 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
     8041 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT
     8101 GACCCCGTAA TTGATTACTA TTAATAACTA GTCAATAATC AATGTCATAA GCTCTAATCG
     8161 CATAATCGAG CTAAGAGCGA TTAATATGCT TAGCTAGCTT AGCTAAGCTA GATTTCTCTA
     8221 TTCTCCCACA ATATATAGCA GCTATTAGCT TAGCAGTCCG AATACGATCG TCCACGATCC
     8281 ATAAGCGTAA CACATTTGAG TAGCGACTGA CGGGCACCGG AGCCACTCGA GTGGAATTAC
     8341 TGACGGGCAC CGGAGCCACT CGAGTGGAAT T
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