LOCUS       CK530_53_TF119_CTCFL    8512 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..2389
                     /label="CTCFL"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             398..2455
                     /label="Translation 398-2455"
                     /translation="MAATEISVLSEQFTKIKELELMPEKGLKEEEKDGVCREKDHRSPSELEAERTSGAFQDSVLEEEVELVLAPSEESEKYILTLQTVHFTSEAVELQDMSLLSIQQQEGVQVVVQQPGPGLLWLEEGPRQSLQQCVAISIQQELYSPQEMEVLQFHALEENVMVASEDSKLAVSLAETAGLIKLEEEQEKNQLLAERTKEQLFFVETMSGDERSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKTKGAKGTFHCDVCMFTSSRMSSFNRHMKTHTSEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHRRYKHTHEKPFKCSMCKYASVEASKLKRHVRSHTGERPFQCCQCSYASRDTYKLKRHMRTHSGEKPYECHICHTRFTQSGTMKIHILQKHGENVPKYQCPHCATIIARKSDLRVHMRNLHAYSAAELKCRYCSAVFHERYALIQHQKTHKNEKRFKCKHCSYACKQERHMTAHIRTHTGEKPFTCLSCNKCFRQKQLLNAHFRKYHDANFIPTVYKCSKCGKGFSRWINLHRHSEKCGSGEAKSAASGKGRRTRKRKQTILKEATKGQKEAAKGWKEAANGDEAAAEEASTTKGEQFPGEMFPVACRETTARVKEEVDEGVTCEMLLNTMDK*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    2394..2415
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2394..2417)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2397..2415)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2401..2417)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2401..2418)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2401..2481
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2406..2415
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2420..2446
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2420..2446)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2447..2455
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2453..2481
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2456..2481)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2459..2481
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     2459..2481
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2459..2481
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2482..2551
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2486..2508
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2509..2528
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference complement(2529..2551)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2552..2555
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2556..2578)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2556..2578)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2556..2578)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2556..2587
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2579..2603
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2588..2621
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2609..2630)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2613..2747
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2622..2777
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2778..5488
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3019..3053)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3065..3080)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3065..3080)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3084..3100)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3289..3308)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3410..3432
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3410..3432
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3471..3489)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3545..3564
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3557..3661
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3662..4522
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3880..3899)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3880..3899)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4693..5281
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5182..5201
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5435..5452
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5489..5577
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5578..8446
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5658..5679
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5694..5724
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5732..5748
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5739..5758
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     5756..5772
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5798..5860
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6036..6117
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6144..6163
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6151..6167
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6198..6217
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6210..6233)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6234..6833)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6719..6738
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6834..6851
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6834..6890)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6900..7643)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7041..7060)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7244..7263
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     7286..7305
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7340..7359)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7644..7667)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7667..7676)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7696..7907)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7908..8287)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8436..8455
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8447..8479
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8480..8512
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8489..8507)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg gcagccactg agatctctgt
      421 cctttctgag caattcacca agatcaaaga actcgagttg atgccggaaa aaggcctgaa
      481 ggaggaggaa aaagacggag tgtgcagaga gaaagaccat cggagcccta gtgagttgga
      541 ggccgagcgt acctctgggg ccttccagga cagcgtcctg gaggaagaag tggagctggt
      601 gctggccccc tcggaggaga gcgagaagta catcctgacc ctgcagacgg tgcacttcac
      661 ttctgaagct gtggagttgc aggatatgag cttgctgagc atacagcagc aagaaggggt
      721 gcaggtggtg gtgcaacagc ctggccctgg gttgctgtgg cttgaggaag ggccccggca
      781 gagcctgcag cagtgtgtgg ccattagtat ccagcaagag ctgtactccc cgcaagagat
      841 ggaggtgttg cagttccacg ctctagagga gaatgtgatg gtggccagtg aagacagtaa
      901 gttagcggtg agcctggctg aaactgctgg actgatcaag ctcgaggaag agcaggagaa
      961 gaaccagtta ttggctgaaa gaacaaagga gcagctcttt tttgtggaaa caatgtcagg
     1021 agatgaaaga agtgacgaaa ttgttctcac agtttcaaat tcaaatgtgg aagaacaaga
     1081 ggatcaacct acagctggtc aagcagatgc tgaaaaggcc aaatctacaa aaaatcaaag
     1141 aaagacaaag ggagcaaaag gaaccttcca ctgtgatgtc tgcatgttca cctcttctag
     1201 aatgtcaagt tttaatcgtc atatgaaaac tcacaccagt gagaagcctc acctgtgtca
     1261 cctctgcctg aaaaccttcc gtacggtcac tctgctgcgg aaccatgtta acacccacac
     1321 aggaaccagg ccctacaagt gtaacgactg caacatggca tttgtcacca gtggagaact
     1381 cgtccgacac aggcgctata aacatactca tgagaaaccc tttaaatgtt ccatgtgcaa
     1441 gtatgccagt gtggaggcaa gtaaattgaa gcgccatgtc cgatcccaca ctggggagcg
     1501 cccctttcag tgttgccagt gcagctatgc cagcagagac acctacaagc tgaaacgcca
     1561 catgagaacg cactcaggtg agaagcctta cgaatgccac atctgccaca cccgcttcac
     1621 ccagagcggg accatgaaaa tacatattct gcagaaacac ggcgaaaatg tccccaaata
     1681 ccagtgtccc cattgtgcca ccatcattgc acggaaaagc gacctacgtg tgcatatgcg
     1741 caacttgcat gcttacagcg ctgcagagct gaaatgccgc tactgttctg ctgtcttcca
     1801 tgaacgctat gccctcattc agcaccagaa aactcataag aatgagaaga ggttcaagtg
     1861 caaacactgc agttatgcct gcaagcagga acgtcatatg accgctcaca ttcgtaccca
     1921 cactggagag aaaccattca cctgcctttc ttgcaataaa tgtttccgac agaagcaact
     1981 tctaaacgct cacttcagga aataccacga tgcaaatttc atcccgactg tttacaaatg
     2041 ctccaagtgt ggcaaaggct tttcccgctg gattaacctg cacagacatt cggagaagtg
     2101 tggatcaggg gaagcaaagt cggctgcttc aggaaaggga agaagaacaa gaaagaggaa
     2161 gcagaccatc ctgaaggaag ccacaaaggg tcagaaggaa gctgcgaagg gatggaagga
     2221 agccgcgaac ggagacgaag ctgctgctga ggaggcttcc accacgaagg gagaacagtt
     2281 cccaggagag atgtttcctg tcgcctgcag agaaaccaca gccagagtca aagaggaagt
     2341 ggatgaaggc gtgacctgtg aaatgctcct caacacgatg gataagtgaT TGGACCCAGC
     2401 TTTCTTGTAC AAAGTGGTAT ACCCATACGA TGTTCCAGAT TACGCTTAGT AATGAGAAAT
     2461 ACTCAGAAGA TGTCACTCAC CATAGTCCGC AGTGAAAGAT AAATGATCAA GAGGCACTTG
     2521 CACTGTCAGT TTTAGAGCTA GAAATAGCAA GTAGGCCACT GAGCAAGAAC TATGAATGAG
     2581 ATCTTTAGAC ATATTGCGGC CGCTTTCTCC GCGATCCAGA CATGATAAGA TACATTGATG
     2641 AGTTTGGACA AACCAAAACT AGAATGCAGT GAAAAAAATG CCTTATTTGT GAAATTTGTG
     2701 ATGCTATTGC CTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTATC TATAACAAGA
     2761 AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT
     2821 ATGAGTTAAA TCTTAAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT
     2881 CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC
     2941 AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG
     3001 AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAAGAA
     3061 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA
     3121 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA
     3181 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT
     3241 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC
     3301 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
     3361 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCATGTCTC CGGGAGCTGC
     3421 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACACGCGA GTTCGAAAGG GCCTCGTGAT
     3481 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC
     3541 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT
     3601 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG
     3661 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC
     3721 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC
     3781 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC
     3841 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC
     3901 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT
     3961 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT
     4021 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT
     4081 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT
     4141 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT
     4201 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC
     4261 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG
     4321 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGTTC
     4381 ACGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA
     4441 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC
     4501 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA
     4561 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT
     4621 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT
     4681 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA
     4741 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA
     4801 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT
     4861 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT
     4921 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA
     4981 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT
     5041 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC
     5101 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA
     5161 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG
     5221 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA
     5281 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT
     5341 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC
     5401 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA
     5461 TGAGCGCCCA ATACGCAAAC CGCCTCTCAC CGCGCGTTGG CCGATTCATT AATGCAGCTG
     5521 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAAACC GCCTCTCCCC
     5581 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
     5641 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
     5701 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
     5761 AACAGCTATG ACCATGATTA CGCCAAGGTC GACTTAACCC TAGAAAGATA ATCATATTGT
     5821 GACGTACGTT AAAGATAATC ATGCGTAAAA TTGACGCATG TGTTTTATCG GTCTGTATAT
     5881 CGAGGTTTAT TTATTAATTT GAATAGATAT TAAGTTTTAT TATATTTACA CTTACATACT
     5941 AATAATAAAT TCAACAAACA ATTTATTTAT GTTTATTTAT TTATTAAAAA AAAACAAAAA
     6001 CTCAAAATTT CTTCTATAAA GTAACAAAAC TTTTAGCAGT GAAAAAAATG CTTTATTTGT
     6061 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
     6121 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
     6181 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTGGA GATTCATGCG
     6241 CCAGGTTTTC TGGTCATGCA CCACGTTCGT GGGCCCTCAG GAACCTCCAC GTCAGCGGTA
     6301 ACTGTGAAGC CCAGTCGCTC GTAAAAAGGA AGATTCCGGG GAGCACTGGT TTCGAGGAAC
     6361 GCCGGCACGC CAGCCCGTTC CGCGGCCTCA ACACCAGGAA GAACGACAGC GCTTCCCAAA
     6421 CCTTTACCTT GGTGATCTGG GCTGACACCG ACAGTGGCAA GAAACCACGC AGGCTCCTTT
     6481 GGTCTATGAG GAGCCAGCAA GCCCTCCATT TGCTGTTGTG CCGCCAACCT ACTCCCTGAC
     6541 AGCTCGGCCA TCCGCGGACC TATTTCCGCG AACACTGCGC CGGCTTCGAC GCTTTCCGGA
     6601 GTAGTCCAGA CCGCAACTGC GGCACCATCG TCCGCAACCC AAACTTTACC AATGTCCAGA
     6661 CCCACGCGCG TAAGAAACAG CTCTTGCAAC TCAGTTACCC TCTCTATGTG TCTGTCCGGA
     6721 TCTACGGTAT GGCGGGTCGC TGGATAATCT GCAAATGCTG CGGCAAGTGT TCGGACAGCC
     6781 CGTGGTACAT CGTCACGGGT AGCGAGCCTA ACAGTGGGCT TATATTCCGT CATGGGGCCG
     6841 GGGTTCTCCT CCACGTCGCC GGCCTGCTTC AGCAGGCTGA AGTTGGTGGC GCCGCTGCCC
     6901 CCGGGGAGCA TGTCAAGGTC AAAATCGTCA AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA
     6961 AAGTCGTCAA GGGCATCGGC TGGGAGCATG TCTAAGTCAA AATCGTCAAG GGCGTCGGTC
     7021 GGCCCGCCGC TTTCGCACTT TAGCTGTTTC TCCAGGCCAC ATATGATTAG TTCCAGGCCG
     7081 AAAAGGAAGG CAGGTTCGGC TCCCTGCCGG TCGAACAGCT CAATTGCTTG TTTCAGAAGT
     7141 GGGGGCATAG AATCGGTGGT AGGTGTCTCT CTTTCCTCTT TTGCTACTTG ATGCTCCTGT
     7201 TCCTCCAATA CGCAGCCCAG TGTAAAGTGG CCCACGGCGG ACAGAGCGTA CAGTGCGTTC
     7261 TCCAGGGAGA AGCCTTGCTG ACACAGGAAC GCGAGCTGAT TTTCCAGGGT TTCGTACTGT
     7321 TTCTCTGTTG GGCGGGTGCC GAGATGCACT TTAGCCCCGT CACGATGTGA GAGGAGAGCA
     7381 CAGCGGTATG ACTTGGCGTT GTTCCGCAGA AAGTCTTGCC ATGACTCGCC TTCCAGGGGG
     7441 CAGGAGTGGG TATGATGCCT GTCCAGCATC TCGATTGGCA GGGCATCGAG CAGGGCCCGC
     7501 TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC TGCTCAACTC CCAGCTTTTG AGCGAGTTTC
     7561 CTTGTCGTCA GGCCTTCGAT ACCGACTCCA TTGAGTAATT CCAGAGCAGA GTTTATGACT
     7621 TTGCTCTTGT CCAGTCTAGA CATCTTATCG TCATCGTCTT TGTAATCCAT GGTGGCGGAT
     7681 CCCGCGTCAC GACACCTGTG TTCTGGCGGC AAACCCGTTG CGAAAAAGAA CGTTCACGGC
     7741 GACTACTGCA CTTATATACG GTTCTCCCCC ACCCTCGGGA AAAAGGCGGA GCCAGTACAC
     7801 GACATCACTT TCCCAGTTTA CCCCGCGCCA CCTTCTCTAG GCACCGGTTC AATTGCCGAC
     7861 CCCTCCCCCC AACTTCTCGG GCACTGTGGG CCATGTGCGC TCTGCCCCAT GGTAATAGCC
     7921 ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTGCC ATAAGGTCAT GTACTGGGCA
     7981 TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA
     8041 TACACTTGAT GTACTGCCAA GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA
     8101 TGGAAAGTGC CTATTGGCGT TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG
     8161 GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC
     8221 ATATATGGGC TATGAACTAA TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT
     8281 CAATGTCATA AGCTCTAATC GCATAATCGA GCTAAGAGCG ATTAATATGC TTAGCTAGCT
     8341 TAGCTAAGCT AGATTTCTCT ATTCTCCCAC AATATATAGC AGCTATTAGC TTAGCAGTCC
     8401 GAATACGATC GTCCACGATC CATAAGCGTA ACACATTTGA GTAGCGACTG ACGGGCACCG
     8461 GAGCCACTCG AGTGGAATTA CTGACGGGCA CCGGAGCCAC TCGAGTGGAA TT
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