LOCUS       CK530_54_TF120_MYBL1    8599 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..2476
                     /label="MYBL1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             398..2542
                     /label="Translation 398-2542"
                     /translation="MAKRSRSEDEDDDLQYADHDYEVPQQKGLKKLWNRVKWTRDEDDKLKKLVEQHGTDDWTLIASHLQNRSDFQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSLIAKHLKGRIGKQCRERWHNHLNPEVKKSSWTEEEDRIIYEAHKRLGNRWAEIAKLLPGRTDNSIKNHWNSTMRRKVEQEGYLQDGIKSERSSSKLQHKPCAAMDHMQTQNQFYIPVQIPGYQYVSPEGNCIEHVQPTSAFIQQPFIDEDPDKEKKIKELEMLLMSAENEVRRKRIPSQPGSFSSWSGSFLMDDNMSNTLNSLDEHTSEFYSMDENQPVSAQQNSPTKFLAVEANAVLSSLQTIPEFAETLELIESDPVAWSDVTSFDISDAAASPIKSTPVKLMRIQHNEGAMECQFNVSLVLEGKKNTCNGGNSEAVPLTSPNIAKFSTPPAILRKKRKMRVGHSPGSELRDGSLNDGGNMALKHTPLKTLPFSPSQFFNTCPGNEQLNIENPSFTSTPICGQKALITTPLHKETTPKDQKENVGFRTPTIRRSILGTTPRTPTPFKNALAAQEKKYGPLKIVSQPLAFLEEDIREVLKEETGTDLFLKEEDEPAYKSCKQENTASGKKVRKSLVLDNWEKEESGTQLLTEDISDMQSNCEWETVVYGKTEDQLIMTEQARRYLSTYTATSSTSRALIL*LDPAFLYKVVYPYDVPDYA***"
     misc_feature    2481..2502
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2481..2504)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2484..2502)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(2488..2504)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(2488..2505)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 2488..2568
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2493..2502
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2507..2533
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2507..2533)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      2534..2542
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2540..2568
                     /label="MPS311_BC_fwd2"
                     /note="sequence: TGAGAAATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2543..2568)
                     /label="MPS312-gBlock_attR2-DD_rev"
                     /note="sequence: GGTGAGTGACATCTTCTGAGTATTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2546..2568
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     2546..2568
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2546..2568
                     /label="MPS287-barcode-fwd"
                     /note="sequence: ATACTCAGAAGATGTCACTCACC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 2569..2638
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 2573..2595
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2596..2615
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_difference complement(2616..2638)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 2639..2642
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2643..2665)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(2643..2665)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2643..2665)
                     /label="MPS288-barcode-rev"
                     /note="sequence: CATTCATAGTTCTTGCTCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 2643..2674
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2666..2690
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2675..2708
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2696..2717)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    2700..2834
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2709..2864
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2865..5575
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3106..3140)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3152..3167)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(3152..3167)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3171..3187)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3376..3395)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     3497..3519
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          3497..3519
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(3558..3576)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3632..3651
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        3644..3748
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3749..4609
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3967..3986)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3967..3986)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      4780..5368
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5269..5288
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5522..5539
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 5576..5664
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5665..8533
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5745..5766
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5781..5811
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5819..5835
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5826..5845
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     5843..5859
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5885..5947
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6123..6204
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6231..6250
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6238..6254
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6285..6304
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6297..6320)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(6321..6920)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6806..6825
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          6921..6938
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6921..6977)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6987..7730)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7128..7147)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          7331..7350
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     7373..7392
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7427..7446)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7731..7754)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7754..7763)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7783..7994)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7995..8374)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     8523..8542
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 8534..8566
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 8567..8599
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(8576..8594)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg gcgaagaggt cgcgcagtga
      421 ggatgaggat gatgaccttc agtatgccga tcatgattat gaagtaccac aacaaaaagg
      481 actgaagaaa ctctggaaca gagtaaaatg gacaagggac gaggatgata aattaaagaa
      541 gttggttgaa caacatggaa ctgatgattg gactctaatt gctagtcatc ttcaaaatcg
      601 ctctgatttt cagtgccagc atcgatggca gaaagtttta aatcctgaat tgataaaggg
      661 tccttggact aaagaagaag atcagagggt tattgaatta gttcagaaat atgggccaaa
      721 aagatggtct ttaattgcaa aacatttaaa aggaagaata ggcaagcagt gtagagaaag
      781 atggcataat catctgaatc ctgaggtaaa gaaatcttcc tggacagaag aggaggacag
      841 gatcatctat gaagcacata agcggttggg aaatcgttgg gcagaaattg ccaaactact
      901 tccaggaagg actgataatt ctatcaaaaa tcattggaat tctactatgc gaagaaaagt
      961 ggaacaggag ggctatttac aagatggaat aaaatcagaa cgatcttcat ctaaacttca
     1021 acacaaacct tgtgcagcta tggatcatat gcaaacccag aatcagtttt acatacctgt
     1081 tcagatccct gggtatcagt atgtgtcacc tgaaggcaat tgtatagaac atgttcagcc
     1141 tacttctgcc tttattcagc aacccttcat tgatgaagat cctgataagg aaaagaaaat
     1201 aaaggaactt gagatgcttc ttatgtcagc tgagaatgaa gttagaagaa agcgaattcc
     1261 atcacagcct ggaagttttt ctagctggtc tggtagtttc ctcatggatg ataacatgtc
     1321 taatactcta aatagccttg acgagcacac tagtgagttt tacagtatgg atgaaaatca
     1381 gcctgtgtct gctcagcaga attcacccac aaagttcctg gccgtggagg caaacgctgt
     1441 gttatcctct ttgcagacca tcccagaatt tgcagagact ctagaactta ttgaatctga
     1501 tcctgtagca tggagtgacg ttaccagttt tgatatttct gatgctgctg cttctcctat
     1561 caaatccacc ccagttaaat taatgagaat tcagcacaat gaaggagcca tggaatgcca
     1621 atttaacgtc agtcttgtac ttgaagggaa aaaaaacact tgtaatggtg gcaacagtga
     1681 agctgttcct ttaacatccc caaatatagc caagtttagc actccaccag ccatcctcag
     1741 aaagaagaga aaaatgcgag tgggtcattc cccaggcagc gaacttaggg atggctcatt
     1801 gaacgatggt ggtaatatgg cgctaaaaca tacaccactg aaaacactac cattttctcc
     1861 ttcacagttt ttcaacacat gtcctggtaa tgaacaactt aatatagaaa atccttcatt
     1921 tacatcaacc cctatttgtg ggcagaaagc tctcattaca actcctcttc ataaggaaac
     1981 aactcccaaa gatcaaaagg aaaatgtagg gtttagaaca cctactatta gaagatctat
     2041 actgggtacc acaccaagaa ctcctactcc ttttaagaat gcgcttgctg ctcaggagaa
     2101 aaaatatgga cctcttaaaa ttgtgtccca gccacttgct ttcttggaag aagatattcg
     2161 ggaagtttta aaagaagaaa ctggaacaga cctattcctc aaagaggaag atgaacctgc
     2221 ttacaaaagc tgcaaacaag agaataccgc ttctgggaag aaagtcagaa aatcactagt
     2281 cttagataat tgggaaaaag aagaatcagg cactcaactg ttgactgaag acatttcaga
     2341 catgcagtca aattgtgaat gggaaacagt ggtttatggg aagacagaag accaacttat
     2401 tatgactgaa caagcaagaa gatatctgag tacttacaca gctaccagta gtacttcaag
     2461 agctctcata ctgtaaTTGG ACCCAGCTTT CTTGTACAAA GTGGTATACC CATACGATGT
     2521 TCCAGATTAC GCTTAGTAAT GAGAAATACT CAGAAGATGT CACTCACCAT AGTCCGCAGT
     2581 GAAAGATAAA TGATCTCTTT GTCGTATGGT GTCAAGTTTT AGAGCTAGAA ATAGCAAGTA
     2641 GGCCACTGAG CAAGAACTAT GAATGAGATC TTTAGACATA TTGCGGCCGC TTTCTCCGCG
     2701 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CAAAACTAGA ATGCAGTGAA
     2761 AAAAATGCCT TATTTGTGAA ATTTGTGATG CTATTGCCTT ATTTGTAACC ATTATAAGCT
     2821 GCAATAAACA AGTTATCTAT AACAAGAAAA TATATATATA ATAAGTTATC ACGTAAGTAG
     2881 AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC GTAAAAGATA
     2941 ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC ACTTACCGCA
     3001 TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA ATGCACAGCG
     3061 ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC GTCAATTTTA
     3121 CGCAGACTAT CTTTCTAGGG TTAAGAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG
     3181 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG
     3241 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
     3301 CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT
     3361 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     3421 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     3481 AGCTGTGACC ATGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA
     3541 CACGCGAGTT CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT
     3601 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
     3661 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
     3721 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
     3781 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
     3841 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
     3901 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
     3961 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
     4021 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
     4081 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
     4141 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
     4201 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
     4261 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
     4321 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
     4381 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
     4441 TAAATCTGGA GCCGGTGAGC GTGGTTCACG CGGTATCATT GCAGCACTGG GGCCAGATGG
     4501 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
     4561 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
     4621 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
     4681 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
     4741 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
     4801 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
     4861 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
     4921 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
     4981 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
     5041 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
     5101 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
     5161 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
     5221 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
     5281 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
     5341 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
     5401 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
     5461 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
     5521 CAGCGAGTCA GTGAGCGAGG AAGCGGATGA GCGCCCAATA CGCAAACCGC CTCTCACCGC
     5581 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
     5641 TGAGCGCAAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC
     5701 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC
     5761 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT
     5821 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGGTCGAC
     5881 TTAACCCTAG AAAGATAATC ATATTGTGAC GTACGTTAAA GATAATCATG CGTAAAATTG
     5941 ACGCATGTGT TTTATCGGTC TGTATATCGA GGTTTATTTA TTAATTTGAA TAGATATTAA
     6001 GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT TATTTATGTT
     6061 TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA ACAAAACTTT
     6121 TAGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     6181 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     6241 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
     6301 GATTATGATC CTCTGGAGAT TCATGCGCCA GGTTTTCTGG TCATGCACCA CGTTCGTGGG
     6361 CCCTCAGGAA CCTCCACGTC AGCGGTAACT GTGAAGCCCA GTCGCTCGTA AAAAGGAAGA
     6421 TTCCGGGGAG CACTGGTTTC GAGGAACGCC GGCACGCCAG CCCGTTCCGC GGCCTCAACA
     6481 CCAGGAAGAA CGACAGCGCT TCCCAAACCT TTACCTTGGT GATCTGGGCT GACACCGACA
     6541 GTGGCAAGAA ACCACGCAGG CTCCTTTGGT CTATGAGGAG CCAGCAAGCC CTCCATTTGC
     6601 TGTTGTGCCG CCAACCTACT CCCTGACAGC TCGGCCATCC GCGGACCTAT TTCCGCGAAC
     6661 ACTGCGCCGG CTTCGACGCT TTCCGGAGTA GTCCAGACCG CAACTGCGGC ACCATCGTCC
     6721 GCAACCCAAA CTTTACCAAT GTCCAGACCC ACGCGCGTAA GAAACAGCTC TTGCAACTCA
     6781 GTTACCCTCT CTATGTGTCT GTCCGGATCT ACGGTATGGC GGGTCGCTGG ATAATCTGCA
     6841 AATGCTGCGG CAAGTGTTCG GACAGCCCGT GGTACATCGT CACGGGTAGC GAGCCTAACA
     6901 GTGGGCTTAT ATTCCGTCAT GGGGCCGGGG TTCTCCTCCA CGTCGCCGGC CTGCTTCAGC
     6961 AGGCTGAAGT TGGTGGCGCC GCTGCCCCCG GGGAGCATGT CAAGGTCAAA ATCGTCAAGA
     7021 GCGTCAGCAG GCAGCATATC AAGGTCAAAG TCGTCAAGGG CATCGGCTGG GAGCATGTCT
     7081 AAGTCAAAAT CGTCAAGGGC GTCGGTCGGC CCGCCGCTTT CGCACTTTAG CTGTTTCTCC
     7141 AGGCCACATA TGATTAGTTC CAGGCCGAAA AGGAAGGCAG GTTCGGCTCC CTGCCGGTCG
     7201 AACAGCTCAA TTGCTTGTTT CAGAAGTGGG GGCATAGAAT CGGTGGTAGG TGTCTCTCTT
     7261 TCCTCTTTTG CTACTTGATG CTCCTGTTCC TCCAATACGC AGCCCAGTGT AAAGTGGCCC
     7321 ACGGCGGACA GAGCGTACAG TGCGTTCTCC AGGGAGAAGC CTTGCTGACA CAGGAACGCG
     7381 AGCTGATTTT CCAGGGTTTC GTACTGTTTC TCTGTTGGGC GGGTGCCGAG ATGCACTTTA
     7441 GCCCCGTCAC GATGTGAGAG GAGAGCACAG CGGTATGACT TGGCGTTGTT CCGCAGAAAG
     7501 TCTTGCCATG ACTCGCCTTC CAGGGGGCAG GAGTGGGTAT GATGCCTGTC CAGCATCTCG
     7561 ATTGGCAGGG CATCGAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG GGTAGGCTGC
     7621 TCAACTCCCA GCTTTTGAGC GAGTTTCCTT GTCGTCAGGC CTTCGATACC GACTCCATTG
     7681 AGTAATTCCA GAGCAGAGTT TATGACTTTG CTCTTGTCCA GTCTAGACAT CTTATCGTCA
     7741 TCGTCTTTGT AATCCATGGT GGCGGATCCC GCGTCACGAC ACCTGTGTTC TGGCGGCAAA
     7801 CCCGTTGCGA AAAAGAACGT TCACGGCGAC TACTGCACTT ATATACGGTT CTCCCCCACC
     7861 CTCGGGAAAA AGGCGGAGCC AGTACACGAC ATCACTTTCC CAGTTTACCC CGCGCCACCT
     7921 TCTCTAGGCA CCGGTTCAAT TGCCGACCCC TCCCCCCAAC TTCTCGGGCA CTGTGGGCCA
     7981 TGTGCGCTCT GCCCCATGGT AATAGCCATG ACTAATACGT AGATGTACTG CCAAGTAGGA
     8041 AAGTGCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG
     8101 TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC
     8161 CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTGCCTA TTGGCGTTAC TATGGGAACA
     8221 TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT
     8281 ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT
     8341 GATTACTATT AATAACTAGT CAATAATCAA TGTCATAAGC TCTAATCGCA TAATCGAGCT
     8401 AAGAGCGATT AATATGCTTA GCTAGCTTAG CTAAGCTAGA TTTCTCTATT CTCCCACAAT
     8461 ATATAGCAGC TATTAGCTTA GCAGTCCGAA TACGATCGTC CACGATCCAT AAGCGTAACA
     8521 CATTTGAGTA GCGACTGACG GGCACCGGAG CCACTCGAGT GGAATTACTG ACGGGCACCG
     8581 GAGCCACTCG AGTGGAATT
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