LOCUS       CK530_TF122_ANHX        7916 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1841..1854)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1841..1855)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1841..1918
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1857..1883
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1884..1892
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1896..1918
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     1896..1918
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 1919..1988
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1923..1945
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1946..1965
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference complement(1966..1988)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1989..1992
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1993..2015)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1993..2015)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 1993..2024
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           2016..2040
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 2025..2058
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    2050..2184
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 2059..2214
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2215..4925
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2456..2490)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2502..2517)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(2726..2745)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2847..2869
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2908..2926)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2994..3098
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3099..3959
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3317..3336)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      4130..4718
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4619..4638
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4872..4889
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4926..5014
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5015..7883
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    5095..5116
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5131..5161
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5169..5185
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     5193..5209
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5235..5297
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5473..5554
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5581..5600
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5671..6270)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6156..6175
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6271..6327)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6337..7080)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6478..6497)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6723..6742
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(7081..7104)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7104..7113)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7133..7344)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7345..7724)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7873..7883
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7884..7916
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7893..7911)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GCAGAGCTTC CTGACTCTGC TGAAGGAGCA
      421 TGAGGACACC TGTGCACCCC CGGCGGAGCT GGTGACCCTT GCGGGCAGAC TGTGCCGGGA
      481 CTTCCAGGAT GACCTTGCCC AACTGCAGCC TTTGGTCACA GCCATTCTGG ACAGCCAGCT
      541 CCGCCTGCAT CTCCTGGACA ACGCAGATGT GGCCCTGGCG TGCGCCCGTG TCCTGGACCA
      601 GCAGGAGCAG CAGCAGGCGG CTTGCCGCCT CCTGGAGGGG TGCCAGGTGC CGGGAGGCAG
      661 CCAGGAGTTA GTGCAGCTCT GGAACGACAT CCACTACCGT CTGGTCATGA GGAGGCTGGG
      721 CGTGGCTGCG CTCACCCCGG TGCAGAAGTT CCGCTGCAGG AAGAGGAACC CCCCGCCCCC
      781 CTCCCTCTGC CCAGAGGGGC TGAAGAGCCG GAACTTCCCC AGAGAGGTTC GTGAGAAGCT
      841 GCACAATTTC GCTGTGGGGG TGAACACCAA CCCCAGCAAG GCTGAGAGGG AGAACTTGGC
      901 ATTGGAGACG AGCTTGACCC CTGAGCAGGT GTACAACTGG TTTGCCAATT ACCGGCGCCG
      961 CCAAAGAGCC CTTCCCCAGC ACATGAAGCC AGCCCAGCAG GCCACAGCTG AAGACCCTGG
     1021 TGCGAGGGAG AGGGGTCCTG ACCTCCTGCA GCCCTCAGGC AACCCCCGTG TTGACTCTGG
     1081 GTTTGTGGAC AGGCCTCAGT GGTCAGAGGA ACGTGAGGAA AAGGGGCCTC CACAGTCCCC
     1141 ACAGACCACC CAAGGACCAT GGGAGCCACT GGCCTTAGCC CCGGACTTTC CCGCAGATGA
     1201 GACAGTCTCA AAGCCACTGG ATGTCAGGTC TCTGCCAGGT GGCGAGATGT ACCAGGAGGG
     1261 GCCTGGCCAT GATCCTGCCA CCCTCCCCCT CTTCTGCCCT GGCCCTGGCC TCTGCCCTCT
     1321 GGCTGCTGGC AATGACATGC TGAACCCCTC TCTGGCTGCC CCTGAATCTT GGCTGATGTC
     1381 TCTTGCACTG GCGTCCTCCA AGGAAGTTTC CTTCCAGACT GGGCAGCTGG TCCACAGCCA
     1441 TGGGCTGGAC TTCATGATGG GCCCTGCAGA CACCGCTGTG GCTGTGTCCA TTGCCACCCT
     1501 TGGAGATCCC AGCCCTACAG GGTTTTCTGG CCCCCCCAGC GGCCATCCCC AGAGCGTGCA
     1561 ATTGGAGGAG GGTCTGGGCA CAAGCAGTGG ACGGACAGAG CTACGGGTGG GCAGCTTCCT
     1621 GGTGACACAG CCCCCACTGC AAGCTCCTGA ATTCATCCTC ACCCAGAGCC CTCCAGAGCT
     1681 GGCCCCAGCC CCATCTGCCT TCCCCGGCCC TGTGTCTGCC ATGGAGCTGA GCCAGGCCCT
     1741 GCCCTCCAGC CAGGTGCAGT GTTCTGATAG CCAGGCCTCT GGTGATGCCT TCTGGGGAGC
     1801 CAGGATGCTC CTTGAGTTTT CAGGGAGCAG CCTGGGCTGA CTTGTACAAA GTGGTATACC
     1861 CATACGATGT TCCAGATTAC GCTTAGTAAT GAGAAATACT CAGAAGATGT CACTCACCAT
     1921 AGTCCGCAGT GAAAGATAAA TGATCTTATG ACTTTGACTT GACAAGTTTT AGAGCTAGAA
     1981 ATAGCAAGTA GGCCACTGAG CAAGAACTAT GAATGAGATC TTTAGACATA TTGCGGCCGC
     2041 TTTCTCCGCG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CAAAACTAGA
     2101 ATGCAGTGAA AAAAATGCCT TATTTGTGAA ATTTGTGATG CTATTGCCTT ATTTGTAACC
     2161 ATTATAAGCT GCAATAAACA AGTTATCTAT AACAAGAAAA TATATATATA ATAAGTTATC
     2221 ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC
     2281 GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC
     2341 ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA
     2401 ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC
     2461 GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAAGAATTC ACTGGCCGTC GTTTTACAAC
     2521 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
     2581 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
     2641 GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT
     2701 CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC
     2761 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
     2821 CTTACAGACA AGCTGTGACC ATGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
     2881 ATCACCGAAA CACGCGAGTT CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG
     2941 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
     3001 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
     3061 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
     3121 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
     3181 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
     3241 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
     3301 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC
     3361 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
     3421 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
     3481 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
     3541 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
     3601 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT
     3661 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
     3721 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
     3781 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGTTCACG CGGTATCATT GCAGCACTGG
     3841 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
     3901 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
     3961 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA
     4021 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT
     4081 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT
     4141 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT
     4201 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC
     4261 AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG
     4321 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG
     4381 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
     4441 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC
     4501 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG
     4561 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG
     4621 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT
     4681 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT
     4741 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG
     4801 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA
     4861 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGATGA GCGCCCAATA CGCAAACCGC
     4921 CTCTCACCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
     4981 AAGCGGGCAG TGAGCGCAAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG
     5041 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT
     5101 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT
     5161 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC
     5221 CAAGGTCGAC TTAACCCTAG AAAGATAATC ATATTGTGAC GTACGTTAAA GATAATCATG
     5281 CGTAAAATTG ACGCATGTGT TTTATCGGTC TGTATATCGA GGTTTATTTA TTAATTTGAA
     5341 TAGATATTAA GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT
     5401 TATTTATGTT TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA
     5461 ACAAAACTTT TAGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
     5521 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
     5581 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG
     5641 TGGTATGGCT GATTATGATC CTCTGGAGAT TCATGCGCCA GGTTTTCTGG TCATGCACCA
     5701 CGTTCGTGGG CCCTCAGGAA CCTCCACGTC AGCGGTAACT GTGAAGCCCA GTCGCTCGTA
     5761 AAAAGGAAGA TTCCGGGGAG CACTGGTTTC GAGGAACGCC GGCACGCCAG CCCGTTCCGC
     5821 GGCCTCAACA CCAGGAAGAA CGACAGCGCT TCCCAAACCT TTACCTTGGT GATCTGGGCT
     5881 GACACCGACA GTGGCAAGAA ACCACGCAGG CTCCTTTGGT CTATGAGGAG CCAGCAAGCC
     5941 CTCCATTTGC TGTTGTGCCG CCAACCTACT CCCTGACAGC TCGGCCATCC GCGGACCTAT
     6001 TTCCGCGAAC ACTGCGCCGG CTTCGACGCT TTCCGGAGTA GTCCAGACCG CAACTGCGGC
     6061 ACCATCGTCC GCAACCCAAA CTTTACCAAT GTCCAGACCC ACGCGCGTAA GAAACAGCTC
     6121 TTGCAACTCA GTTACCCTCT CTATGTGTCT GTCCGGATCT ACGGTATGGC GGGTCGCTGG
     6181 ATAATCTGCA AATGCTGCGG CAAGTGTTCG GACAGCCCGT GGTACATCGT CACGGGTAGC
     6241 GAGCCTAACA GTGGGCTTAT ATTCCGTCAT GGGGCCGGGG TTCTCCTCCA CGTCGCCGGC
     6301 CTGCTTCAGC AGGCTGAAGT TGGTGGCGCC GCTGCCCCCG GGGAGCATGT CAAGGTCAAA
     6361 ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG TCGTCAAGGG CATCGGCTGG
     6421 GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGTCGGC CCGCCGCTTT CGCACTTTAG
     6481 CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA AGGAAGGCAG GTTCGGCTCC
     6541 CTGCCGGTCG AACAGCTCAA TTGCTTGTTT CAGAAGTGGG GGCATAGAAT CGGTGGTAGG
     6601 TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGTTCC TCCAATACGC AGCCCAGTGT
     6661 AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC AGGGAGAAGC CTTGCTGACA
     6721 CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC TCTGTTGGGC GGGTGCCGAG
     6781 ATGCACTTTA GCCCCGTCAC GATGTGAGAG GAGAGCACAG CGGTATGACT TGGCGTTGTT
     6841 CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG GAGTGGGTAT GATGCCTGTC
     6901 CAGCATCTCG ATTGGCAGGG CATCGAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG
     6961 GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT GTCGTCAGGC CTTCGATACC
     7021 GACTCCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG CTCTTGTCCA GTCTAGACAT
     7081 CTTATCGTCA TCGTCTTTGT AATCCATGGT GGCGGATCCC GCGTCACGAC ACCTGTGTTC
     7141 TGGCGGCAAA CCCGTTGCGA AAAAGAACGT TCACGGCGAC TACTGCACTT ATATACGGTT
     7201 CTCCCCCACC CTCGGGAAAA AGGCGGAGCC AGTACACGAC ATCACTTTCC CAGTTTACCC
     7261 CGCGCCACCT TCTCTAGGCA CCGGTTCAAT TGCCGACCCC TCCCCCCAAC TTCTCGGGCA
     7321 CTGTGGGCCA TGTGCGCTCT GCCCCATGGT AATAGCCATG ACTAATACGT AGATGTACTG
     7381 CCAAGTAGGA AAGTGCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC
     7441 GTCATTGACG TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG
     7501 GGCAGTTTAC CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTGCCTA TTGGCGTTAC
     7561 TATGGGAACA TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG
     7621 CGGGCCATTT ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA
     7681 CCCCGTAATT GATTACTATT AATAACTAGT CAATAATCAA TGTCATAAGC TCTAATCGCA
     7741 TAATCGAGCT AAGAGCGATT AATATGCTTA GCTAGCTTAG CTAAGCTAGA TTTCTCTATT
     7801 CTCCCACAAT ATATAGCAGC TATTAGCTTA GCAGTCCGAA TACGATCGTC CACGATCCAT
     7861 AAGCGTAACA CATTTGAGTA GCGACTGACG GGCACCGGAG CCACTCGAGT GGAATT
//