LOCUS       CK530_TF123_RB1         9251 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(3176..3189)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(3176..3190)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 3176..3253
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3192..3218
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      3219..3227
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     3231..3253
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     3231..3253
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 3254..3323
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 3258..3280
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3281..3300
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_difference complement(3301..3323)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 3324..3327
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3328..3350)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3328..3350)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 3328..3359
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           3351..3375
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 3360..3393
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    3385..3519
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 3394..3549
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 3550..6260
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(3791..3825)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3837..3852)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(4061..4080)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     4182..4204
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(4243..4261)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        4329..4433
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4434..5294
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4652..4671)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      5465..6053
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     5954..5973
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6207..6224
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 6261..6349
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 6350..9218
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    6430..6451
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        6466..6496
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    6504..6520
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     6528..6544
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   6570..6632
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    6808..6889
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6916..6935
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(7006..7605)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     7491..7510
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(7606..7662)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(7672..8415)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(7813..7832)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     8058..8077
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(8416..8439)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(8439..8448)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(8468..8679)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(8680..9059)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     9208..9218
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 9219..9251
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(9228..9246)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GCCACCGAAG ACACCAAGGA AGACTGCGGC
      421 AACTGCTGCT GCCGCAGCTG CCGAGCCACC AGCTCCTCCT CCACCTCCTC CCCCAGAAGA
      481 AGATCCTGAA CAAGATTCTG GGCCAGAAGA TCTTCCCCTG GTAAGACTCG AATTCGAGGA
      541 GACTGAGGAG CCCGACTTCA CAGCTCTGTG CCAAAAGCTC AAAATTCCTG ACCACGTTCG
      601 AGAACGAGCA TGGCTGACAT GGGAAAAGGT CAGCTCCGTC GACGGTGTCC TCGGCGGCTA
      661 CATACAGAAG AAGAAAGAGC TCTGGGGCAT ATGCATTTTC ATCGCCGCTG TCGATTTAGA
      721 CGAAATGTCT TTTACCTTCA CCGAATTGCA AAAGAATATC GAGATTTCTG TGCACAAGTT
      781 CTTCAATCTG CTGAAGGAGA TAGACACTTC TACAAAGGTC GACAACGCCA TGAGTCGGCT
      841 TCTGAAGAAA TACGACGTGC TCTTCGCCTT ATTTTCTAAG CTGGAGCGAA CTTGCGAGCT
      901 GATTTACCTG ACGCAGCCTT CTTCAAGCAT CTCCACAGAG ATCAACAGTG CTCTGGTTCT
      961 CAAGGTCAGC TGGATTACTT TCCTGCTTGC AAAGGGTGAG GTCCTGCAGA TGGAAGACGA
     1021 CCTTGTTATC AGTTTCCAAT TGATGTTGTG CGTTTTGGAT TACTTCATCA AGCTTTCTCC
     1081 GCCGATGCTC CTGAAGGAGC CCTACAAGAC TGCCGTAATC CCAATCAACG GCAGCCCACG
     1141 TACTCCTCGT CGGGGCCAAA ATCGTAGCGC CCGCATCGCC AAGCAGCTCG AGAACGACAC
     1201 TCGCATAATC GAGGTCCTGT GCAAGGAGCA CGAGTGCAAC ATCGACGAAG TTAAGAACGT
     1261 GTACTTTAAG AACTTCATTC CATTCATGAA CTCACTCGGC TTAGTGACCT CAAACGGTCT
     1321 GCCTGAAGTC GAGAACCTGT CCAAGAGGTA TGAGGAGATC TACCTGAAGA ACAAGGACCT
     1381 CGACGCTCGG CTGTTCCTCG ACCACGACAA GACACTGCAA ACAGACTCAA TTGATAGCTT
     1441 CGAGACGCAA CGAACCCCCA GGAAGTCCAA TCTCGACGAG GAAGTCAACG TCATACCCCC
     1501 TCATACGCCT GTGCGGACGG TGATGAATAC CATACAGCAG CTGATGATGA TACTGAACTC
     1561 TGCATCCGAC CAGCCGTCTG AGAACCTTAT AAGTTACTTC AATAATTGTA CCGTCAACCC
     1621 CAAGGAGAGC ATTTTGAAGC GGGTTAAAGA CATTGGTTAT ATATTCAAGG AAAAGTTCGC
     1681 AAAGGCCGTC GGTCAAGGAT GCGTGGAGAT AGGTTCCCAA CGTTATAAGT TGGGTGTGAG
     1741 GCTTTACTAT AGAGTGATGG AGTCAATGCT GAAGAGCGAG GAAGAGCGGC TGAGTATCCA
     1801 GAACTTCTCC AAGCTCCTCA ACGATAATAT CTTCCACATG TCCTTGCTGG CATGTGCACT
     1861 GGAAGTGGTT ATGGCGACCT ACTCAAGGTC TACTAGCCAA AACCTGGACT CCGGCACTGA
     1921 CCTCAGTTTT CCTTGGATCC TCAACGTCCT GAACCTTAAG GCTTTCGACT TCTATAAGGT
     1981 AATTGAGAGC TTCATTAAGG CTGAGGGAAA TCTCACCAGG GAGATGATTA AGCACTTGGA
     2041 GAGATGCGAG CACCGGATAA TGGAGTCTCT GGCTTGGCTT AGTGACAGCC CACTGTTCGA
     2101 CCTGATCAAG CAGAGCAAAG ATAGAGAGGG TCCGACCGAC CATTTAGAGA GTGCCTGCCC
     2161 CCTCAACCTG CCATTGCAAA ACAACCATAC CGCCGCCGAC ATGTACCTGT CCCCCGTGAG
     2221 GTCACCCAAG AAGAAGGGCA GTACCACACG CGTTAACAGT ACAGCTAACG CGGAAACTCA
     2281 GGCTACTTCC GCATTTCAAA CTCAGAAACC GCTGAAGAGC ACAAGCCTGA GCCTTTTCTA
     2341 CAAGAAGGTT TACCGCTTGG CTTACCTGCG ACTGAACACC TTGTGCGAGA GGCTGCTCAG
     2401 CGAACATCCT GAGTTGGAGC ACATAATTTG GACACTCTTT CAACATACAT TACAAAACGA
     2461 ATACGAGTTG ATGAGGGATC GCCACCTTGA TCAGATCATG ATGTGCAGTA TGTACGGGAT
     2521 CTGTAAGGTT AAGAACATCG ATCTGAAGTT TAAGATAATC GTGACTGCTT ATAAAGACTT
     2581 ACCACACGCC GTGCAAGAAA CCTTTAAGCG GGTGCTGATT AAAGAGGAAG AATACGACTC
     2641 AATAATTGTC TTTTACAATT CTGTGTTTAT GCAAAGGCTT AAGACGAACA TCCTTCAATA
     2701 CGCCTCTACA AGACCACCCA CTCTCAGCCC CATTCCCCAT ATCCCACGCT CTCCCTATAA
     2761 ATTCCCAAGC TCTCCACTGC GCATCCCGGG TGGTAATATT TACATCAGTC CGTTGAAAAG
     2821 CCCCTACAAG ATAAGCGAGG GCCTGCCCAC CCCGACGAAG ATGACACCGC GGTCCCGCAT
     2881 ACTTGTCAGC ATCGGAGAGT CCTTTGGCAC ATCAGAAAAG TTTCAAAAGA TTAACCAAAT
     2941 GGTCTGCAAT TCTGATAGAG TACTGAAGCG CTCTGCCGAG GGCTCTAATC CCCCAAAGCC
     3001 TTTGAAGAAG CTGAGGTTCG ACATCGAGGG GAGTGACGAG GCCGACGGGT CTAAGCACCT
     3061 TCCTGGGGAA AGCAAGTTCC AACAAAAGCT TGCCGAGATG ACTTCCACCC GCACCAGGAT
     3121 GCAGAAACAA AAGATGAACG ACTCCATGGA CACATCCAAT AAAGAGGAAA AGTAACTTGT
     3181 ACAAAGTGGT ATACCCATAC GATGTTCCAG ATTACGCTTA GTAATGAGAA ATACTCAGAA
     3241 GATGTCACTC ACCATAGTCC GCAGTGAAAG ATAAATGATC ACCTTACTTA AAATCGTTTC
     3301 GTTTTAGAGC TAGAAATAGC AAGTAGGCCA CTGAGCAAGA ACTATGAATG AGATCTTTAG
     3361 ACATATTGCG GCCGCTTTCT CCGCGATCCA GACATGATAA GATACATTGA TGAGTTTGGA
     3421 CAAACCAAAA CTAGAATGCA GTGAAAAAAA TGCCTTATTT GTGAAATTTG TGATGCTATT
     3481 GCCTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA TCTATAACAA GAAAATATAT
     3541 ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA
     3601 AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG
     3661 TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG
     3721 ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT
     3781 TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAG AATTCACTGG
     3841 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG
     3901 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT
     3961 CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC
     4021 ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG
     4081 CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC
     4141 TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCATGTC TCCGGGAGCT GCATGTGTCA
     4201 GAGGTTTTCA CCGTCATCAC CGAAACACGC GAGTTCGAAA GGGCCTCGTG ATACGCCTAT
     4261 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
     4321 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
     4381 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
     4441 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
     4501 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
     4561 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
     4621 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG
     4681 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
     4741 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
     4801 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
     4861 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
     4921 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG
     4981 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
     5041 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
     5101 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGT TCACGCGGTA
     5161 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
     5221 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
     5281 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
     5341 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
     5401 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
     5461 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
     5521 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
     5581 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC
     5641 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
     5701 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
     5761 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
     5821 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
     5881 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
     5941 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
     6001 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
     6061 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
     6121 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
     6181 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GATGAGCGCC
     6241 CAATACGCAA ACCGCCTCTC ACCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
     6301 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAA CCGCCTCTCC CCGCGCGTTG
     6361 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     6421 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
     6481 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
     6541 TGACCATGAT TACGCCAAGG TCGACTTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
     6601 TTAAAGATAA TCATGCGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
     6661 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
     6721 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAAACAAA AACTCAAAAT
     6781 TTCTTCTATA AAGTAACAAA ACTTTTAGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG
     6841 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA
     6901 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
     6961 AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTG GAGATTCATG CGCCAGGTTT
     7021 TCTGGTCATG CACCACGTTC GTGGGCCCTC AGGAACCTCC ACGTCAGCGG TAACTGTGAA
     7081 GCCCAGTCGC TCGTAAAAAG GAAGATTCCG GGGAGCACTG GTTTCGAGGA ACGCCGGCAC
     7141 GCCAGCCCGT TCCGCGGCCT CAACACCAGG AAGAACGACA GCGCTTCCCA AACCTTTACC
     7201 TTGGTGATCT GGGCTGACAC CGACAGTGGC AAGAAACCAC GCAGGCTCCT TTGGTCTATG
     7261 AGGAGCCAGC AAGCCCTCCA TTTGCTGTTG TGCCGCCAAC CTACTCCCTG ACAGCTCGGC
     7321 CATCCGCGGA CCTATTTCCG CGAACACTGC GCCGGCTTCG ACGCTTTCCG GAGTAGTCCA
     7381 GACCGCAACT GCGGCACCAT CGTCCGCAAC CCAAACTTTA CCAATGTCCA GACCCACGCG
     7441 CGTAAGAAAC AGCTCTTGCA ACTCAGTTAC CCTCTCTATG TGTCTGTCCG GATCTACGGT
     7501 ATGGCGGGTC GCTGGATAAT CTGCAAATGC TGCGGCAAGT GTTCGGACAG CCCGTGGTAC
     7561 ATCGTCACGG GTAGCGAGCC TAACAGTGGG CTTATATTCC GTCATGGGGC CGGGGTTCTC
     7621 CTCCACGTCG CCGGCCTGCT TCAGCAGGCT GAAGTTGGTG GCGCCGCTGC CCCCGGGGAG
     7681 CATGTCAAGG TCAAAATCGT CAAGAGCGTC AGCAGGCAGC ATATCAAGGT CAAAGTCGTC
     7741 AAGGGCATCG GCTGGGAGCA TGTCTAAGTC AAAATCGTCA AGGGCGTCGG TCGGCCCGCC
     7801 GCTTTCGCAC TTTAGCTGTT TCTCCAGGCC ACATATGATT AGTTCCAGGC CGAAAAGGAA
     7861 GGCAGGTTCG GCTCCCTGCC GGTCGAACAG CTCAATTGCT TGTTTCAGAA GTGGGGGCAT
     7921 AGAATCGGTG GTAGGTGTCT CTCTTTCCTC TTTTGCTACT TGATGCTCCT GTTCCTCCAA
     7981 TACGCAGCCC AGTGTAAAGT GGCCCACGGC GGACAGAGCG TACAGTGCGT TCTCCAGGGA
     8041 GAAGCCTTGC TGACACAGGA ACGCGAGCTG ATTTTCCAGG GTTTCGTACT GTTTCTCTGT
     8101 TGGGCGGGTG CCGAGATGCA CTTTAGCCCC GTCACGATGT GAGAGGAGAG CACAGCGGTA
     8161 TGACTTGGCG TTGTTCCGCA GAAAGTCTTG CCATGACTCG CCTTCCAGGG GGCAGGAGTG
     8221 GGTATGATGC CTGTCCAGCA TCTCGATTGG CAGGGCATCG AGCAGGGCCC GCTTGTTCTT
     8281 CACGTGCCAG TACAGGGTAG GCTGCTCAAC TCCCAGCTTT TGAGCGAGTT TCCTTGTCGT
     8341 CAGGCCTTCG ATACCGACTC CATTGAGTAA TTCCAGAGCA GAGTTTATGA CTTTGCTCTT
     8401 GTCCAGTCTA GACATCTTAT CGTCATCGTC TTTGTAATCC ATGGTGGCGG ATCCCGCGTC
     8461 ACGACACCTG TGTTCTGGCG GCAAACCCGT TGCGAAAAAG AACGTTCACG GCGACTACTG
     8521 CACTTATATA CGGTTCTCCC CCACCCTCGG GAAAAAGGCG GAGCCAGTAC ACGACATCAC
     8581 TTTCCCAGTT TACCCCGCGC CACCTTCTCT AGGCACCGGT TCAATTGCCG ACCCCTCCCC
     8641 CCAACTTCTC GGGCACTGTG GGCCATGTGC GCTCTGCCCC ATGGTAATAG CCATGACTAA
     8701 TACGTAGATG TACTGCCAAG TAGGAAAGTG CCATAAGGTC ATGTACTGGG CATAATGCCA
     8761 GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG
     8821 ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT
     8881 GCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT
     8941 GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG
     9001 GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA ATCAATGTCA
     9061 TAAGCTCTAA TCGCATAATC GAGCTAAGAG CGATTAATAT GCTTAGCTAG CTTAGCTAAG
     9121 CTAGATTTCT CTATTCTCCC ACAATATATA GCAGCTATTA GCTTAGCAGT CCGAATACGA
     9181 TCGTCCACGA TCCATAAGCG TAACACATTT GAGTAGCGAC TGACGGGCAC CGGAGCCACT
     9241 CGAGTGGAAT T
//