LOCUS       CK530_TF127_SMC3       10118 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(4043..4056)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(4043..4057)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 4043..4120
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4059..4085
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      4086..4094
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4098..4120
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     4098..4120
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 4121..4190
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 4125..4147
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4148..4167
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_difference complement(4168..4190)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4191..4194
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4195..4217)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4195..4217)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 4195..4226
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           4218..4242
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 4227..4260
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4252..4386
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 4261..4416
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 4417..7127
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(4658..4692)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4704..4719)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(4928..4947)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     5049..5071
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(5110..5128)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5196..5300
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5301..6161
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(5519..5538)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      6332..6920
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     6821..6840
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     7074..7091
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 7128..7216
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 7217..10085
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    7297..7318
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        7333..7363
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    7371..7387
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     7395..7411
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   7437..7499
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    7675..7756
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     7783..7802
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(7873..8472)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     8358..8377
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(8473..8529)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(8539..9282)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(8680..8699)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     8925..8944
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(9283..9306)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(9306..9315)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(9335..9546)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(9547..9926)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     10075..10085
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 10086..10118
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(10095..10113)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GTACATAAAG CAGGTGATTA TCCAGGGTTT
      421 TCGAAGTTAC AGAGATCAAA CAATTGTAGA TCCCTTCAGT TCAAAACATA ATGTGATTGT
      481 GGGCAGAAAT GGATCTGGAA AAAGTAACTT TTTTTATGCA ATTCAGTTTG TTCTCAGTGA
      541 TGAGTTTAGT CATCTTCGTC CAGAACAGCG GTTGGCTTTA TTGCATGAAG GTACTGGTCC
      601 TCGTGTTATT TCTGCTTTTG TGGAGATTAT TTTTGATAAT TCAGACAACC GGTTACCAAT
      661 CGATAAAGAG GAAGTTTCAC TTCGAAGAGT TATTGGTGCC AAAAAGGATC AGTATTTCTT
      721 AGACAAGAAG ATGGTCACGA AAAATGATGT GATGAACCTC CTTGAAAGCG CTGGTTTTTC
      781 TCGAAGCAAT CCTTATTATA TTGTTAAACA AGGAAAGATC AACCAGATGG CAACAGCACC
      841 AGATTCTCAG AGATTAAAGC TATTAAGAGA AGTAGCTGGT ACTAGAGTGT ATGACGAACG
      901 AAAGGAAGAA AGCATCTCCT TAATGAAAGA AACAGAGGGC AAACGGGAAA AAATCAATGA
      961 GTTGTTAAAA TACATTGAAG AGAGATTACA TACTCTAGAG GAAGAAAAGG AAGAACTAGC
     1021 TCAGTATCAG AAGTGGGATA AAATGAGACG AGCCCTGGAA TATACCATTT ACAATCAGGA
     1081 ACTTAACGAG ACTCGTGCCA AACTTGATGA GCTTTCTGCT AAGCGAGAGA CTAGTGGAGA
     1141 AAAATCCAGA CAATTAAGAG ATGCTCAGCA GGATGCAAGA GATAAAATGG AGGATATCGA
     1201 ACGCCAAGTT AGAGAATTGA AAACAAAAAT TTCAGCTATG AAAGAAGAAA AAGAACAGCT
     1261 TAGTGCTGAA AGACAAGAGC AGATTAAGCA GAGGACTAAG TTGGAGCTTA AAGCCAAGGA
     1321 TTTACAAGAT GAACTAGCAG GCAATAGTGA ACAAAGGAAA CGTTTATTAA AAGAGAGGCA
     1381 GAAGCTGCTT GAAAAAATAG AAGAAAAGCA GAAAGAACTG GCAGAAACAG AACCCAAATT
     1441 CAACAGTGTG AAAGAGAAAG AAGAACGAGG AATTGCTAGA TTGGCTCAAG CTACCCAGGA
     1501 AAGAACGGAT CTTTATGCAA AGCAGGGTCG AGGAAGCCAG TTTACATCAA AAGAAGAAAG
     1561 GGATAAGTGG ATTAAAAAGG AACTCAAGTC TTTAGATCAG GCTATTAATG ACAAGAAAAG
     1621 ACAGATTGCT GCTATACATA AGGATTTGGA AGACACTGAA GCAAATAAAG AGAAAAATCT
     1681 GGAGCAGTAT AATAAACTGG ACCAGGATCT TAATGAAGTC AAAGCTCGAG TAGAAGAACT
     1741 GGACAGAAAA TATTACGAAG TAAAAAATAA GAAAGATGAA CTACAAAGTG AAAGAAACTA
     1801 CTTGTGGAGA GAAGAGAATG CAGAACAGCA AGCACTTGCT GCTAAAAGAG AAGATCTTGA
     1861 AAAGAAGCAA CAACTTCTTA GAGCAGCAAC AGGAAAGGCC ATTTTAAATG GAATAGACAG
     1921 CATAAACAAA GTGCTAGACC ACTTCCGTCG AAAAGGAATA AACCAGCATG TTCAAAATGG
     1981 CTATCATGGT ATTGTAATGA ATAACTTTGA ATGTGAACCA GCTTTCTACA CATGCGTGGA
     2041 AGTCACTGCT GGAAACAGGT TATTTTATCA CATTGTTGAT TCAGATGAAG TCAGCACGAA
     2101 GATTTTAATG GAGTTTAATA AAATGAATCT TCCTGGAGAG GTTACTTTTC TGCCTCTTAA
     2161 CAAGTTAGAT GTCAGGGATA CAGCCTATCC TGAAACCAAT GATGCTATTC CTATGATCAG
     2221 CAAACTGAGG TACAATCCCA GATTTGACAA AGCTTTCAAA CATGTGTTTG GAAAGACTCT
     2281 TATTTGTCGT AGCATGGAAG TTTCAACCCA GCTGGCCCGT GCTTTCACTA TGGACTGTAT
     2341 TACTTTGGAA GGTGACCAAG TCAGCCATCG GGGTGCTCTA ACTGGGGGTT ATTATGACAC
     2401 AAGGAAGTCT CGACTTGAAT TGCAAAAAGA TGTTAGAAAA GCAGAAGAAG AACTAGGTGA
     2461 ACTTGAAGCA AAGCTCAATG AAAACCTGCG CAGAAATATT GAAAGGATTA ATAATGAAAT
     2521 TGATCAGTTG ATGAACCAAA TGCAACAGAT CGAGACCCAG CAAAGGAAAT TTAAAGCATC
     2581 TAGAGATAGC ATATTATCAG AAATGAAGAT GCTAAAAGAG AAGAGGCAGC AGTCAGAGAA
     2641 AACCTTCATG CCTAAGCAAC GTAGCTTACA GAGTTTGGAG GCAAGCTTGC ATGCTATGGA
     2701 GTCTACCAGA GAGTCATTGA AAGCAGAACT GGGAACTGAT TTGCTTTCTC AACTGAGTTT
     2761 GGAAGATCAG AAGAGAGTAG ATGCACTGAA TGATGAGATT CGTCAACTTC AGCAGGAAAA
     2821 CAGACAGTTG CTAAATGAAA GAATTAAATT AGAAGGTATT ATTACTCGAG TAGAGACTTA
     2881 TCTCAATGAG AATCTGAGAA AACGCTTGGA CCAAGTAGAA CAGGAACTTA ATGAGCTGAG
     2941 AGAGACAGAA GGGGGTACTG TTCTCACAGC CACAACATCA GAACTTGAAG CCATCAATAA
     3001 AAGAGTAAAA GACACTATGG CACGATCAGA AGATTTGGAC AATTCCATTG ATAAAACAGA
     3061 AGCTGGAATT AAGGAGCTTC AGAAGAGTAT GGAGCGCTGG AAAAATATGG AAAAAGAACA
     3121 TATGGATGCT ATAAATCATG ATACTAAAGA ACTGGAAAAG ATGACAAATC GGCAAGGCAT
     3181 GCTATTGAAG AAGAAAGAAG AGTGTATGAA GAAAATTCGA GAACTTGGAT CACTTCCCCA
     3241 GGAAGCATTT GAAAAGTACC AGACACTGAG CCTCAAACAG TTGTTTCGAA AACTTGAGCA
     3301 GTGCAACACA GAATTAAAGA AGTACAGCCA TGTTAACAAA AAGGCTTTGG ATCAGTTTGT
     3361 AAATTTCTCC GAGCAGAAAG AAAAGTTAAT AAAGCGTCAA GAAGAGTTAG ATAGGGGTTA
     3421 CAAATCAATC ATGGAACTGA TGAATGTACT TGAACTTCGG AAATATGAAG CTATTCAGTT
     3481 AACTTTCAAA CAGGTATCTA AGAACTTCAG TGAAGTATTC CAGAAGTTAG TACCTGGTGG
     3541 CAAAGCTACT TTGGTGATGA AGAAAGGAGA TGTGGAGGGC AGTCAGTCTC AAGATGAAGG
     3601 AGAAGGGAGT GGTGAGAGTG AGAGGGGTTC TGGCTCACAA AGCAGTGTCC CATCAGTTGA
     3661 CCAGTTTACT GGAGTTGGAA TTAGGGTGTC ATTTACAGGA AAACAAGGTG AAATGAGAGA
     3721 AATGCAACAG CTTTCAGGTG GACAGAAATC CTTGGTAGCC CTTGCTCTGA TTTTTGCCAT
     3781 TCAGAAATGT GACCCGGCTC CATTTTACTT GTTTGATGAA ATTGACCAGG CTCTGGATGC
     3841 TCAGCACAGA AAGGCTGTGT CAGATATGAT TATGGAACTT GCTGTACATG CTCAGTTTAT
     3901 TACAACTACT TTTAGGCCTG AACTGCTTGA GTCAGCTGAC AAATTCTATG GTGTAAAGTT
     3961 CAGAAATAAG GTTAGTCATA TTGATGTGAT CACAGCAGAG ATGGCCAAAG ACTTTGTAGA
     4021 AGATGATACC ACACATGGTT AACTTGTACA AAGTGGTATA CCCATACGAT GTTCCAGATT
     4081 ACGCTTAGTA ATGAGAAATA CTCAGAAGAT GTCACTCACC ATAGTCCGCA GTGAAAGATA
     4141 AATGATCTGC TTGTGAGTTT GGTGGATGTT TTAGAGCTAG AAATAGCAAG TAGGCCACTG
     4201 AGCAAGAACT ATGAATGAGA TCTTTAGACA TATTGCGGCC GCTTTCTCCG CGATCCAGAC
     4261 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCAAAACTA GAATGCAGTG AAAAAAATGC
     4321 CTTATTTGTG AAATTTGTGA TGCTATTGCC TTATTTGTAA CCATTATAAG CTGCAATAAA
     4381 CAAGTTATCT ATAACAAGAA AATATATATA TAATAAGTTA TCACGTAAGT AGAACATGAA
     4441 ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC ACGTAAAAGA TAATCATGCG
     4501 TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG ACACTTACCG CATTGACAAG
     4561 CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT AAATGCACAG CGACGGATTC
     4621 GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT GCGTCAATTT TACGCAGACT
     4681 ATCTTTCTAG GGTTAAGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC
     4741 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA
     4801 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC
     4861 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA
     4921 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
     4981 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
     5041 CCATGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACACGCGAG
     5101 TTCGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
     5161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
     5221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     5281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     5341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     5401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     5461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     5521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     5581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     5641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     5701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     5761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     5821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     5881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     5941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     6001 GAGCCGGTGA GCGTGGTTCA CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     6061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     6121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     6181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     6241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     6301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     6361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     6421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC
     6481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     6541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     6601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     6661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     6721 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     6781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     6841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     6901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     6961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
     7021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
     7081 CAGTGAGCGA GGAAGCGGAT GAGCGCCCAA TACGCAAACC GCCTCTCACC GCGCGTTGGC
     7141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
     7201 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     7261 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA
     7321 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
     7381 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGGTCG ACTTAACCCT
     7441 AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA TGCGTAAAAT TGACGCATGT
     7501 GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG AATAGATATT AAGTTTTATT
     7561 ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA TTTATTTATG TTTATTTATT
     7621 TATTAAAAAA AAACAAAAAC TCAAAATTTC TTCTATAAAG TAACAAAACT TTTAGCAGTG
     7681 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
     7741 CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA
     7801 GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
     7861 TCCTCTGGAG ATTCATGCGC CAGGTTTTCT GGTCATGCAC CACGTTCGTG GGCCCTCAGG
     7921 AACCTCCACG TCAGCGGTAA CTGTGAAGCC CAGTCGCTCG TAAAAAGGAA GATTCCGGGG
     7981 AGCACTGGTT TCGAGGAACG CCGGCACGCC AGCCCGTTCC GCGGCCTCAA CACCAGGAAG
     8041 AACGACAGCG CTTCCCAAAC CTTTACCTTG GTGATCTGGG CTGACACCGA CAGTGGCAAG
     8101 AAACCACGCA GGCTCCTTTG GTCTATGAGG AGCCAGCAAG CCCTCCATTT GCTGTTGTGC
     8161 CGCCAACCTA CTCCCTGACA GCTCGGCCAT CCGCGGACCT ATTTCCGCGA ACACTGCGCC
     8221 GGCTTCGACG CTTTCCGGAG TAGTCCAGAC CGCAACTGCG GCACCATCGT CCGCAACCCA
     8281 AACTTTACCA ATGTCCAGAC CCACGCGCGT AAGAAACAGC TCTTGCAACT CAGTTACCCT
     8341 CTCTATGTGT CTGTCCGGAT CTACGGTATG GCGGGTCGCT GGATAATCTG CAAATGCTGC
     8401 GGCAAGTGTT CGGACAGCCC GTGGTACATC GTCACGGGTA GCGAGCCTAA CAGTGGGCTT
     8461 ATATTCCGTC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG GCCTGCTTCA GCAGGCTGAA
     8521 GTTGGTGGCG CCGCTGCCCC CGGGGAGCAT GTCAAGGTCA AAATCGTCAA GAGCGTCAGC
     8581 AGGCAGCATA TCAAGGTCAA AGTCGTCAAG GGCATCGGCT GGGAGCATGT CTAAGTCAAA
     8641 ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT TTCGCACTTT AGCTGTTTCT CCAGGCCACA
     8701 TATGATTAGT TCCAGGCCGA AAAGGAAGGC AGGTTCGGCT CCCTGCCGGT CGAACAGCTC
     8761 AATTGCTTGT TTCAGAAGTG GGGGCATAGA ATCGGTGGTA GGTGTCTCTC TTTCCTCTTT
     8821 TGCTACTTGA TGCTCCTGTT CCTCCAATAC GCAGCCCAGT GTAAAGTGGC CCACGGCGGA
     8881 CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA GCCTTGCTGA CACAGGAACG CGAGCTGATT
     8941 TTCCAGGGTT TCGTACTGTT TCTCTGTTGG GCGGGTGCCG AGATGCACTT TAGCCCCGTC
     9001 ACGATGTGAG AGGAGAGCAC AGCGGTATGA CTTGGCGTTG TTCCGCAGAA AGTCTTGCCA
     9061 TGACTCGCCT TCCAGGGGGC AGGAGTGGGT ATGATGCCTG TCCAGCATCT CGATTGGCAG
     9121 GGCATCGAGC AGGGCCCGCT TGTTCTTCAC GTGCCAGTAC AGGGTAGGCT GCTCAACTCC
     9181 CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG GCCTTCGATA CCGACTCCAT TGAGTAATTC
     9241 CAGAGCAGAG TTTATGACTT TGCTCTTGTC CAGTCTAGAC ATCTTATCGT CATCGTCTTT
     9301 GTAATCCATG GTGGCGGATC CCGCGTCACG ACACCTGTGT TCTGGCGGCA AACCCGTTGC
     9361 GAAAAAGAAC GTTCACGGCG ACTACTGCAC TTATATACGG TTCTCCCCCA CCCTCGGGAA
     9421 AAAGGCGGAG CCAGTACACG ACATCACTTT CCCAGTTTAC CCCGCGCCAC CTTCTCTAGG
     9481 CACCGGTTCA ATTGCCGACC CCTCCCCCCA ACTTCTCGGG CACTGTGGGC CATGTGCGCT
     9541 CTGCCCCATG GTAATAGCCA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTGCCA
     9601 TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG
     9661 GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA
     9721 CTCCACCCAT TGACGTCAAT GGAAAGTGCC TATTGGCGTT ACTATGGGAA CATACGTCAT
     9781 TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG
     9841 TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA TTGATTACTA
     9901 TTAATAACTA GTCAATAATC AATGTCATAA GCTCTAATCG CATAATCGAG CTAAGAGCGA
     9961 TTAATATGCT TAGCTAGCTT AGCTAAGCTA GATTTCTCTA TTCTCCCACA ATATATAGCA
    10021 GCTATTAGCT TAGCAGTCCG AATACGATCG TCCACGATCC ATAAGCGTAA CACATTTGAG
    10081 TAGCGACTGA CGGGCACCGG AGCCACTCGA GTGGAATT
//