LOCUS       CK530_TF128_KDM5B      11099 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(5024..5037)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(5024..5038)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 5024..5101
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5040..5066
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      5067..5075
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     5079..5101
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     5079..5101
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 5102..5171
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 5106..5128
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5129..5148
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_difference complement(5149..5171)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 5172..5175
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(5176..5198)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(5176..5198)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 5176..5207
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           5199..5223
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 5208..5241
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    5233..5367
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 5242..5397
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 5398..8108
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(5639..5673)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5685..5700)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(5909..5928)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     6030..6052
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(6091..6109)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        6177..6281
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             6282..7142
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(6500..6519)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      7313..7901
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     7802..7821
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     8055..8072
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 8109..8197
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 8198..11066
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    8278..8299
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        8314..8344
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    8352..8368
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     8376..8392
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   8418..8480
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    8656..8737
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     8764..8783
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(8854..9453)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     9339..9358
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(9454..9510)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(9520..10263)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(9661..9680)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     9906..9925
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(10264..10287)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(10287..10296)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(10316..10527)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(10528..10907)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     11056..11066
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 11067..11099
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(11076..11094)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GGAAGCTGCA ACTACGTTAC ATCCTGGACC
      421 CAGACCCGCT CTTCCGCTGG GTGGACCCGG ACCTCTCGGA GAATTTCTTC CCCCGCCAGA
      481 ATGTCCTGTG TTTGAGCCAA GTTGGGAAGA ATTTGCCGAT CCTTTTGCCT TTATTCATAA
      541 AATTAGACCA ATCGCTGAAC AAACAGGTAT TTGCAAAGTC CGCCCACCCC CTGACTGGCA
      601 ACCGCCCTTC GCTTGCGACG TGGACAAGCT CCACTTCACC CCGAGGATTC AAAGGCTCAA
      661 CGAGCTGGAA GCGCAGACCA GAGTGAAGCT CAACTTTCTG GATCAAATAG CGAAATATTG
      721 GGAATTGCAA GGTTCCACCT TAAAGATCCC TCACGTTGAA CGCAAAATTC TCGATCTGTT
      781 CCAATTAAAC AAACTGGTGG CTGAGGAGGG AGGGTTCGCC GTCGTATGTA AAGACAGGAA
      841 GTGGACAAAG ATCGCAACCA AAATGGGATT CGCCCCAGGA AAGGCCGTCG GTTCCCACAT
      901 ACGTGGCCAC TACGAGCGGA TATTGAATCC TTATAATCTC TTTCTCAGCG GGGATTCACT
      961 GCGGTGCCTT CAAAAGCCCA ATCTCACGAC CGATACCAAA GATAAAGAAT ATAAGCCTCA
     1021 CGACATCCCA CAACGACAAT CAGTCCAACC GTCTGAGACA TGTCCGCCCG CTCGGCGGGC
     1081 GAAGCGGATG CGTGCTGAAG CTATGAACAT AAAGATCGAG CCAGAAGAAA CTACTGAGGC
     1141 TCGGACCCAC AACCTTCGCC GAAGGATGGG GTGCCCTACC CCTAAGTGCG AGAACGAAAA
     1201 GGAGATGAAA TCCAGCATTA AACAGGAGCC CATCGAACGG AAGGACTACA TCGTCGAGAA
     1261 CGAGAAAGAG AAACCTAAAT CAAGGTCAAA GAAGGCAACT AACGCGGTCG ATTTATACGT
     1321 GTGCTTGCTG TGCGGGTCTG GGAACGACGA GGATCGCCTG TTACTGTGCG ACGGATGCGA
     1381 CGATTCCTAT CACACTTTCT GTCTTATTCC GCCACTTCAC GACGTACCTA AAGGTGATTG
     1441 GAGATGCCCC AAATGCCTCG CACAAGAGTG CTCCAAACCC CAGGAAGCGT TCGGTTTCGA
     1501 GCAGGCCGCA AGAGATTACA CTTTGCGCAC CTTCGGAGAG ATGGCCGACG CATTTAAGTC
     1561 CGACTATTTT AATATGCCTG TTCACATGGT ACCGACCGAA CTGGTGGAAA AGGAGTTCTG
     1621 GCGTCTGGTC TCTACCATCG AAGAAGACGT TACCGTCGAG TACGGGGCAG ATATCGCTTC
     1681 CAAAGAGTTC GGTAGCGGTT TCCCAGTGAG AGACGGAAAG ATTAAGCTGA GTCCGGAAGA
     1741 GGAAGAATAC CTGGACAGCG GATGGAACCT GAATAATATG CCTGTTATGG AACAAAGCGT
     1801 ACTCGCCCAC ATCACAGCCG ACATCTGCGG AATGAAGCTG CCCTGGCTGT ACGTAGGGAT
     1861 GTGTTTCAGT AGCTTTTGCT GGCATATCGA GGATCATTGG TCTTACAGCA TCAATTATCT
     1921 GCATTGGGGC GAACCGAAGA CTTGGTACGG GGTTCCTGGA TACGCCGCCG AACAACTGGA
     1981 GAACGTCATG AAGAAGCTCG CACCTGAGCT GTTCGTCTCA CAACCCGACT TGCTGCACCA
     2041 ACTGGTCACA ATTATGAATC CAAACACATT GATGACGCAC GAGGTTCCAG TCTATCGTAC
     2101 GAACCAATGC GCCGGAGAAT TCGTCATCAC TTTCCCCCGG GCGTATCATT CTGGCTTCAA
     2161 TCAAGGGTTC AACTTCGCCG AAGCCGTGAA CTTTTGTACG GTAGACTGGC TCCCTCTGGG
     2221 TAGACAATGC GTCGAACACT ACAGACTTCT GCACAGATAC TGCGTATTCA GTCATGACGA
     2281 AATGATATGT AAAATGGCCT CAAAAGCCGA CGTGTTGGAC GTCGTTGTCG CCAGTACCGT
     2341 GCAAAAGGAT ATGGCTATCA TGATCGAAGA CGAAAAGGCC CTGCGGGAGA CCGTACGGAA
     2401 GCTCGGGGTC ATAGACAGCG AGAGGATGGA CTTCGAACTT CTCCCCGACG ACGAGCGACA
     2461 ATGCGTCAAG TGTAAGACGA CTTGTTTTAT GTCAGCAATT AGCTGCTCCT GCAAGCCGGG
     2521 GTTGTTAGTA TGTCTTCACC ACGTGAAGGA GTTATGCTCT TGCCCACCAT ATAAGTACAA
     2581 GCTGCGATAC CGATATACTC TTGACGACCT GTATCCAATG ATGAACGCCC TCAAACTCAG
     2641 AGCGGAGTCC TATAATGAGT GGGCTCTGAA CGTAAACGAG GCACTGGAAG CCAAAATTAA
     2701 TAAGAAGAAG TCCCTGGTGT CTTTTAAAGC CCTTATCGAG GAGAGTGAGA TGAAGAAGTT
     2761 TCCTGATAAC GACCTGTTAC GGCATCTGAG GCTGGTTACC CAAGACGCTG AAAAGTGCGC
     2821 TAGCGTGGCC CAACAATTAC TGAACGGAAA GCGCCAGACA CGATACAGAA GTGGGGGGGG
     2881 TAAGAGCCAG AACCAACTGA CCGTTAACGA ACTTCGTCAA TTCGTCACTC AATTGTACGC
     2941 CTTGCCTTGC GTGCTGAGCC AAACTCCCCT TCTTAAAGAC CTGCTGAACC GCGTGGAAGA
     3001 CTTCCAGCAA CACTCACAAA AGCTCTTATC AGAAGAGACC CCCAGCGCCG CAGAACTTCA
     3061 AGATCTGCTG GACGTATCAT TCGAGTTCGA CGTGGAGCTG CCTCAACTGG CCGAAATGAG
     3121 GATTCGCCTG GAGCAGGCGC GGTGGCTTGA GGAAGTTCAA CAGGCCTGTT TAGATCCTAG
     3181 TAGCCTGACG CTGGACGACA TGCGCCGCCT TATTGATCTT GGCGTGGGTC TCGCACCTTA
     3241 CTCCGCTGTA GAAAAGGCCA TGGCAAGGCT TCAAGAGTTG TTAACCGTTA GCGAACATTG
     3301 GGATGATAAG GCTAAAAGCC TGTTAAAAGC GCGGCCTCGC CACTCCCTTA ACAGTCTGGC
     3361 CACAGCTGTG AAAGAGATTG AGGAAATTCC AGCCTATCTC CCTAACGGCG CTGCGTTGAA
     3421 GGATAGTGTC CAACGGGCGC GCGATTGGCT GCAAGACGTG GAAGGGTTGC AAGCCGGCGG
     3481 CAGGGTTCCT GTTCTTGATA CTCTTATCGA GCTCGTCACC CGCGGGCGTA GCATACCGGT
     3541 GCACCTTAAC TCACTTCCTC GGTTGGAGAC TCTGGTGGCC GAAGTGCAAG CCTGGAAGGA
     3601 GTGCGCCGTA AACACGTTTC TCACAGAAAA CAGTCCTTAC TCCCTGCTTG AAGTCCTCTG
     3661 CCCAAGGTGC GACATCGGTC TGCTGGGCTT AAAGAGAAAA CAACGAAAAC TCAAAGAACC
     3721 ACTGCCCAAC GGGAAGAAGA AGTCTACTAA GCTGGAAAGC TTAAGCGATT TGGAAAGGGC
     3781 GCTGACAGAG TCTAAAGAAA CCGCCAGCGC CATGGCTACA CTGGGAGAGG CCCGGCTCCG
     3841 AGAGATGGAA GCTCTTCAAT CCCTGCGTCT TGCAAACGAG GGCAAGCTGC TCTCACCGCT
     3901 GCAGGACGTT GACATTAAGA TATGTCTGTG CCAGAAAGCA CCTGCCGCGC CCATGATCCA
     3961 GTGCGAGCTG TGTAGAGACG CGTTTCATAC ATCATGCGTT GCTGTGCCAT CAATCAGTCA
     4021 AGGACTTCGC ATTTGGTTGT GCCCGCACTG CCGCAGAAGT GAAAAGCCGC CCCTGGAAAA
     4081 GATCTTGCCT TTGCTTGCTT CTTTGCAAAG AATACGCGTC CGGCTCCCAG AAGGGGACGC
     4141 CTTGAGGTAC ATGATAGAGA GGACAGTAAA TTGGCAACAT AGGGCACAAC AGTTACTGTC
     4201 ATCCGGAAAC CTGAAGTTCG TTCAGGACAG AGTTGGGTCT GGCCTCTTAT ACTCACGATG
     4261 GCAGGCAAGC GCCGGGCAAG TCTCTGATAC CAATAAAGTG AGTCAGCCAC CAGGAACTAC
     4321 GTCCTTCTCC CTTCCCGACG ATTGGGATAA TCGGACGAGT TACCTGCATA GCCCATTTAG
     4381 CACAGGTAGG TCCTGCATTC CTCTGCACGG GGTGAGCCCT GAGGTAAACG AGCTTTTAAT
     4441 GGAAGCGCAA TTACTTCAAG TGTCACTCCC AGAGATCCAA GAGTTGTATC AAACACTGTT
     4501 AGCCAAACCC TCACCAGCCC AACAAACAGA TCGCTCAAGC CCCGTACGCC CAAGTAGCGA
     4561 GAAGAACGAT TGCTGTAGAG GTAAACGCGA CGGTATCAAT AGCCTGGAAA GGAAGCTTAA
     4621 ACGGAGATTG GAGCGAGAAG GGCTGTCTTC AGAACGCTGG GAGCGCGTGA AGAAGATGAG
     4681 GACACCTAAG AAGAAGAAGA TTAAGCTTTC TCATCCTAAA GATATGAATA ACTTTAAACT
     4741 TGAACGCGAA CGAAGTTACG AGTTGGTACG AAGCGCCGAG ACACACAGTC TCCCTAGCGA
     4801 TACCTCTTAC AGTGAGCAAG AGGATAGCGA AGACGAGGAC GCTATATGTC CGGCCGTCTC
     4861 CTGTCTTCAA CCTGAGGGAG ACGAAGTTGA TTGGGTTCAA TGCGACGGTA GTTGTAACCA
     4921 ATGGTTCCAC CAAGTTTGCG TAGGGGTATC TCCTGAAATG GCCGAGAAAG AGGATTATAT
     4981 ATGCGTTCGT TGCACCGTCA AAGATGCCCC CTCTCGCAAA TAGCTTGTAC AAAGTGGTAT
     5041 ACCCATACGA TGTTCCAGAT TACGCTTAGT AATGAGAAAT ACTCAGAAGA TGTCACTCAC
     5101 CATAGTCCGC AGTGAAAGAT AAATGATCAC TGCGCTGTCT TTTTCAAAGT TTTAGAGCTA
     5161 GAAATAGCAA GTAGGCCACT GAGCAAGAAC TATGAATGAG ATCTTTAGAC ATATTGCGGC
     5221 CGCTTTCTCC GCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCAAAACT
     5281 AGAATGCAGT GAAAAAAATG CCTTATTTGT GAAATTTGTG ATGCTATTGC CTTATTTGTA
     5341 ACCATTATAA GCTGCAATAA ACAAGTTATC TATAACAAGA AAATATATAT ATAATAAGTT
     5401 ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAA TCTTAAAAGT
     5461 CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT
     5521 GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC
     5581 TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA
     5641 TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAAGAA TTCACTGGCC GTCGTTTTAC
     5701 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC
     5761 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC
     5821 GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA
     5881 TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC
     5941 AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT
     6001 CCGCTTACAG ACAAGCTGTG ACCATGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC
     6061 GTCATCACCG AAACACGCGA GTTCGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA
     6121 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     6181 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     6241 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     6301 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     6361 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     6421 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     6481 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     6541 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     6601 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     6661 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     6721 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     6781 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA
     6841 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     6901 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     6961 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGTTC ACGCGGTATC ATTGCAGCAC
     7021 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     7081 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     7141 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
     7201 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
     7261 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     7321 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     7381 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     7441 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     7501 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     7561 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     7621 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     7681 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     7741 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     7801 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     7861 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     7921 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     7981 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     8041 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA TGAGCGCCCA ATACGCAAAC
     8101 CGCCTCTCAC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     8161 GGAAAGCGGG CAGTGAGCGC AACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA
     8221 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA
     8281 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT
     8341 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA
     8401 CGCCAAGGTC GACTTAACCC TAGAAAGATA ATCATATTGT GACGTACGTT AAAGATAATC
     8461 ATGCGTAAAA TTGACGCATG TGTTTTATCG GTCTGTATAT CGAGGTTTAT TTATTAATTT
     8521 GAATAGATAT TAAGTTTTAT TATATTTACA CTTACATACT AATAATAAAT TCAACAAACA
     8581 ATTTATTTAT GTTTATTTAT TTATTAAAAA AAAACAAAAA CTCAAAATTT CTTCTATAAA
     8641 GTAACAAAAC TTTTAGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
     8701 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
     8761 TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA
     8821 ATGTGGTATG GCTGATTATG ATCCTCTGGA GATTCATGCG CCAGGTTTTC TGGTCATGCA
     8881 CCACGTTCGT GGGCCCTCAG GAACCTCCAC GTCAGCGGTA ACTGTGAAGC CCAGTCGCTC
     8941 GTAAAAAGGA AGATTCCGGG GAGCACTGGT TTCGAGGAAC GCCGGCACGC CAGCCCGTTC
     9001 CGCGGCCTCA ACACCAGGAA GAACGACAGC GCTTCCCAAA CCTTTACCTT GGTGATCTGG
     9061 GCTGACACCG ACAGTGGCAA GAAACCACGC AGGCTCCTTT GGTCTATGAG GAGCCAGCAA
     9121 GCCCTCCATT TGCTGTTGTG CCGCCAACCT ACTCCCTGAC AGCTCGGCCA TCCGCGGACC
     9181 TATTTCCGCG AACACTGCGC CGGCTTCGAC GCTTTCCGGA GTAGTCCAGA CCGCAACTGC
     9241 GGCACCATCG TCCGCAACCC AAACTTTACC AATGTCCAGA CCCACGCGCG TAAGAAACAG
     9301 CTCTTGCAAC TCAGTTACCC TCTCTATGTG TCTGTCCGGA TCTACGGTAT GGCGGGTCGC
     9361 TGGATAATCT GCAAATGCTG CGGCAAGTGT TCGGACAGCC CGTGGTACAT CGTCACGGGT
     9421 AGCGAGCCTA ACAGTGGGCT TATATTCCGT CATGGGGCCG GGGTTCTCCT CCACGTCGCC
     9481 GGCCTGCTTC AGCAGGCTGA AGTTGGTGGC GCCGCTGCCC CCGGGGAGCA TGTCAAGGTC
     9541 AAAATCGTCA AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC
     9601 TGGGAGCATG TCTAAGTCAA AATCGTCAAG GGCGTCGGTC GGCCCGCCGC TTTCGCACTT
     9661 TAGCTGTTTC TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC
     9721 TCCCTGCCGG TCGAACAGCT CAATTGCTTG TTTCAGAAGT GGGGGCATAG AATCGGTGGT
     9781 AGGTGTCTCT CTTTCCTCTT TTGCTACTTG ATGCTCCTGT TCCTCCAATA CGCAGCCCAG
     9841 TGTAAAGTGG CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG
     9901 ACACAGGAAC GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC
     9961 GAGATGCACT TTAGCCCCGT CACGATGTGA GAGGAGAGCA CAGCGGTATG ACTTGGCGTT
    10021 GTTCCGCAGA AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGGAGTGGG TATGATGCCT
    10081 GTCCAGCATC TCGATTGGCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA
    10141 CAGGGTAGGC TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT
    10201 ACCGACTCCA TTGAGTAATT CCAGAGCAGA GTTTATGACT TTGCTCTTGT CCAGTCTAGA
    10261 CATCTTATCG TCATCGTCTT TGTAATCCAT GGTGGCGGAT CCCGCGTCAC GACACCTGTG
    10321 TTCTGGCGGC AAACCCGTTG CGAAAAAGAA CGTTCACGGC GACTACTGCA CTTATATACG
    10381 GTTCTCCCCC ACCCTCGGGA AAAAGGCGGA GCCAGTACAC GACATCACTT TCCCAGTTTA
    10441 CCCCGCGCCA CCTTCTCTAG GCACCGGTTC AATTGCCGAC CCCTCCCCCC AACTTCTCGG
    10501 GCACTGTGGG CCATGTGCGC TCTGCCCCAT GGTAATAGCC ATGACTAATA CGTAGATGTA
    10561 CTGCCAAGTA GGAAAGTGCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
    10621 ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA
    10681 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTGC CTATTGGCGT
    10741 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
    10801 AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA
    10861 TGACCCCGTA ATTGATTACT ATTAATAACT AGTCAATAAT CAATGTCATA AGCTCTAATC
    10921 GCATAATCGA GCTAAGAGCG ATTAATATGC TTAGCTAGCT TAGCTAAGCT AGATTTCTCT
    10981 ATTCTCCCAC AATATATAGC AGCTATTAGC TTAGCAGTCC GAATACGATC GTCCACGATC
    11041 CATAAGCGTA ACACATTTGA GTAGCGACTG ACGGGCACCG GAGCCACTCG AGTGGAATT
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