LOCUS       CK530_TF130_MSX1        7376 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1301..1314)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1301..1315)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1301..1378
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1317..1343
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1344..1352
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1356..1378
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1356..1378
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1379..1448
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1383..1405
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1406..1425
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_difference complement(1426..1448)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1449..1452
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1453..1475)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1453..1475)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 1453..1484
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1476..1500
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1485..1518
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    1510..1644
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1519..1674
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1675..4385
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(1916..1950)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(1962..1977)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(2186..2205)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2307..2329
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2368..2386)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2454..2558
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2559..3419
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2777..2796)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      3590..4178
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4079..4098
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4332..4349
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4386..4474
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4475..7343
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4555..4576
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4591..4621
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4629..4645
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4653..4669
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4695..4757
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4933..5014
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5041..5060
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5131..5730)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5616..5635
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5731..5787)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5797..6540)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(5938..5957)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6183..6202
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(6541..6564)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6564..6573)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6593..6804)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(6805..7184)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7333..7343
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7344..7376
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7353..7371)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GGCCCCCGCG GCAGATATGA CCTCTCTTCC
      421 CTTAGGAGTT AAGGTCGAAG ATTCTGCGTT TGGTAAACCC GCTGGTGGCG GGGCCGGGCA
      481 AGCTCCTTCC GCTGCTGCTG CGACTGCTGC GGCAATGGGA GCAGATGAAG AAGGAGCTAA
      541 ACCAAAGGTC AGCCCCAGTC TGCTTCCATT TTCCGTCGAA GCACTGATGG CAGATCATAG
      601 AAAACCCGGC GCTAAAGAAA GTGCACTCGC CCCATCTGAA GGAGTCCAAG CAGCCGGCGG
      661 AAGTGCTCAA CCTCTCGGAG TTCCCCCAGG TTCCCTTGGC GCGCCAGATG CCCCAAGCTC
      721 TCCTAGACCC TTGGGGCATT TTAGTGTCGG TGGCCTTCTG AAACTTCCCG AGGACGCTCT
      781 GGTGAAGGCT GAATCCCCTG AGAAACCAGA AAGAACGCCA TGGATGCAAT CTCCACGGTT
      841 TTCACCACCA CCCGCTAGAA GGCTCTCCCC GCCTGCATGT ACTTTAAGGA AGCATAAAAC
      901 TAATAGAAAA CCTAGAACCC CTTTTACTAC AGCACAACTT CTCGCTCTCG AACGTAAATT
      961 TAGGCAGAAA CAATATCTCA GTATTGCTGA ACGGGCTGAA TTTTCAAGTT CCCTGTCACT
     1021 TACAGAAACA CAAGTCAAAA TCTGGTTTCA AAATCGACGA GCGAAAGCCA AACGACTTCA
     1081 GGAAGCCGAA CTCGAAAAGT TGAAAATGGC AGCTAAACCT ATGCTTCCGC CTGCAGCTTT
     1141 TGGACTTAGT TTTCCACTGG GCGGACCAGC CGCAGTGGCA GCTGCCGCCG GCGCAAGCTT
     1201 GTATGGAGCT TCCGGGCCGT TTCAAAGAGC AGCATTACCG GTTGCACCAG TCGGCCTGTA
     1261 TACTGCACAC GTAGGTTATT CCATGTATCA TTTAACTTGA CTTGTACAAA GTGGTATACC
     1321 CATACGATGT TCCAGATTAC GCTTAGTAAT GAGAAATACT CAGAAGATGT CACTCACCAT
     1381 AGTCCGCAGT GAAAGATAAA TGATCCATAT TTAGTTCTCC TTCTTGTTTT AGAGCTAGAA
     1441 ATAGCAAGTA GGCCACTGAG CAAGAACTAT GAATGAGATC TTTAGACATA TTGCGGCCGC
     1501 TTTCTCCGCG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CAAAACTAGA
     1561 ATGCAGTGAA AAAAATGCCT TATTTGTGAA ATTTGTGATG CTATTGCCTT ATTTGTAACC
     1621 ATTATAAGCT GCAATAAACA AGTTATCTAT AACAAGAAAA TATATATATA ATAAGTTATC
     1681 ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC
     1741 GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC
     1801 ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA
     1861 ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC
     1921 GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAAGAATTC ACTGGCCGTC GTTTTACAAC
     1981 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
     2041 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
     2101 GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT
     2161 CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC
     2221 CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG
     2281 CTTACAGACA AGCTGTGACC ATGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC
     2341 ATCACCGAAA CACGCGAGTT CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG
     2401 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
     2461 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
     2521 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
     2581 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
     2641 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
     2701 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
     2761 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC
     2821 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
     2881 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
     2941 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
     3001 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
     3061 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT
     3121 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
     3181 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
     3241 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGTTCACG CGGTATCATT GCAGCACTGG
     3301 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
     3361 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
     3421 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA
     3481 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT
     3541 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT
     3601 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT
     3661 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC
     3721 AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG
     3781 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG
     3841 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
     3901 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC
     3961 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG
     4021 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG
     4081 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT
     4141 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT
     4201 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG
     4261 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA
     4321 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGATGA GCGCCCAATA CGCAAACCGC
     4381 CTCTCACCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
     4441 AAGCGGGCAG TGAGCGCAAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG
     4501 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT
     4561 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT
     4621 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC
     4681 CAAGGTCGAC TTAACCCTAG AAAGATAATC ATATTGTGAC GTACGTTAAA GATAATCATG
     4741 CGTAAAATTG ACGCATGTGT TTTATCGGTC TGTATATCGA GGTTTATTTA TTAATTTGAA
     4801 TAGATATTAA GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT
     4861 TATTTATGTT TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA
     4921 ACAAAACTTT TAGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
     4981 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
     5041 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG
     5101 TGGTATGGCT GATTATGATC CTCTGGAGAT TCATGCGCCA GGTTTTCTGG TCATGCACCA
     5161 CGTTCGTGGG CCCTCAGGAA CCTCCACGTC AGCGGTAACT GTGAAGCCCA GTCGCTCGTA
     5221 AAAAGGAAGA TTCCGGGGAG CACTGGTTTC GAGGAACGCC GGCACGCCAG CCCGTTCCGC
     5281 GGCCTCAACA CCAGGAAGAA CGACAGCGCT TCCCAAACCT TTACCTTGGT GATCTGGGCT
     5341 GACACCGACA GTGGCAAGAA ACCACGCAGG CTCCTTTGGT CTATGAGGAG CCAGCAAGCC
     5401 CTCCATTTGC TGTTGTGCCG CCAACCTACT CCCTGACAGC TCGGCCATCC GCGGACCTAT
     5461 TTCCGCGAAC ACTGCGCCGG CTTCGACGCT TTCCGGAGTA GTCCAGACCG CAACTGCGGC
     5521 ACCATCGTCC GCAACCCAAA CTTTACCAAT GTCCAGACCC ACGCGCGTAA GAAACAGCTC
     5581 TTGCAACTCA GTTACCCTCT CTATGTGTCT GTCCGGATCT ACGGTATGGC GGGTCGCTGG
     5641 ATAATCTGCA AATGCTGCGG CAAGTGTTCG GACAGCCCGT GGTACATCGT CACGGGTAGC
     5701 GAGCCTAACA GTGGGCTTAT ATTCCGTCAT GGGGCCGGGG TTCTCCTCCA CGTCGCCGGC
     5761 CTGCTTCAGC AGGCTGAAGT TGGTGGCGCC GCTGCCCCCG GGGAGCATGT CAAGGTCAAA
     5821 ATCGTCAAGA GCGTCAGCAG GCAGCATATC AAGGTCAAAG TCGTCAAGGG CATCGGCTGG
     5881 GAGCATGTCT AAGTCAAAAT CGTCAAGGGC GTCGGTCGGC CCGCCGCTTT CGCACTTTAG
     5941 CTGTTTCTCC AGGCCACATA TGATTAGTTC CAGGCCGAAA AGGAAGGCAG GTTCGGCTCC
     6001 CTGCCGGTCG AACAGCTCAA TTGCTTGTTT CAGAAGTGGG GGCATAGAAT CGGTGGTAGG
     6061 TGTCTCTCTT TCCTCTTTTG CTACTTGATG CTCCTGTTCC TCCAATACGC AGCCCAGTGT
     6121 AAAGTGGCCC ACGGCGGACA GAGCGTACAG TGCGTTCTCC AGGGAGAAGC CTTGCTGACA
     6181 CAGGAACGCG AGCTGATTTT CCAGGGTTTC GTACTGTTTC TCTGTTGGGC GGGTGCCGAG
     6241 ATGCACTTTA GCCCCGTCAC GATGTGAGAG GAGAGCACAG CGGTATGACT TGGCGTTGTT
     6301 CCGCAGAAAG TCTTGCCATG ACTCGCCTTC CAGGGGGCAG GAGTGGGTAT GATGCCTGTC
     6361 CAGCATCTCG ATTGGCAGGG CATCGAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG
     6421 GGTAGGCTGC TCAACTCCCA GCTTTTGAGC GAGTTTCCTT GTCGTCAGGC CTTCGATACC
     6481 GACTCCATTG AGTAATTCCA GAGCAGAGTT TATGACTTTG CTCTTGTCCA GTCTAGACAT
     6541 CTTATCGTCA TCGTCTTTGT AATCCATGGT GGCGGATCCC GCGTCACGAC ACCTGTGTTC
     6601 TGGCGGCAAA CCCGTTGCGA AAAAGAACGT TCACGGCGAC TACTGCACTT ATATACGGTT
     6661 CTCCCCCACC CTCGGGAAAA AGGCGGAGCC AGTACACGAC ATCACTTTCC CAGTTTACCC
     6721 CGCGCCACCT TCTCTAGGCA CCGGTTCAAT TGCCGACCCC TCCCCCCAAC TTCTCGGGCA
     6781 CTGTGGGCCA TGTGCGCTCT GCCCCATGGT AATAGCCATG ACTAATACGT AGATGTACTG
     6841 CCAAGTAGGA AAGTGCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC
     6901 GTCATTGACG TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG
     6961 GGCAGTTTAC CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTGCCTA TTGGCGTTAC
     7021 TATGGGAACA TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG
     7081 CGGGCCATTT ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA
     7141 CCCCGTAATT GATTACTATT AATAACTAGT CAATAATCAA TGTCATAAGC TCTAATCGCA
     7201 TAATCGAGCT AAGAGCGATT AATATGCTTA GCTAGCTTAG CTAAGCTAGA TTTCTCTATT
     7261 CTCCCACAAT ATATAGCAGC TATTAGCTTA GCAGTCCGAA TACGATCGTC CACGATCCAT
     7321 AAGCGTAACA CATTTGAGTA GCGACTGACG GGCACCGGAG CCACTCGAGT GGAATT
//