LOCUS       CK530_TF131_YTHDC2     10767 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    389..4681
                     /label="YTHDC2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             389..4681
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSRPSSVSPRQPAPGGGGGGGPSPCGPGGGGRAKGLKDIRIDEEVKIAVNIALERFRYGDQREMEFPSSLTSTERAFIHRLSQSLGLVSKSKGKGANRYLTVKKKDGSETAHAMMTCNLTHNTKHAVRSLIQRFPVTNKERTELLPKTERGNVFAVEAENREMSKTSGRLNNGIPQIPVKRGESEFDSFRQSLPVFEKQEEIVKIIKENKVVLIVGETGSGKTTQIPQFLLDDCFKNGIPCRIFCTQPRRLAAIAVAERVAAERRERIGQTIGYQIRLESRVSPKTLLTFCTNGVLLRTLMAGDSTLSTVTHVIVDEVHERDRFSDFLLTKLRDLLQKHPTLKLILSSAALDVNLFIRYFGSCPVIYIQGRPFEVKEMFLEDILRTTGYTNKEMLKYKKEKQQEEKQQTTLTEWYSAQENSFKPESQRQRTVLNVTDEYDLLDDGGDAVFSQLTEKDVNCLEPWLIKEMDACLSDIWLHKDIDAFAQVFHLILTENVSVDYRHSETSATALMVAAGRGFASQVEQLISMGANVHSKASNGWMALDWAKHFGQTEIVDLLESYSATLEFGNLDESSLVQTNGSDLSAEDRELLKAYHHSFDDEKVDLDLIMHLLYNICHSCDAGAVLIFLPGYDEIVGLRDRILFDDKRFADSTHRYQVFMLHSNMQTSDQKKVLKNPPAGVRKIILSTNIAETSITVNDVVFVIDSGKVKEKSFDALNFVTMLKMVWISKASAIQRKGRAGRCRPGICFRLFSRLRFQNMLEFQTPELLRMPLQELCLHTKLLAPVNCPIADFLMKAPEPPPALIVRNAVQMLKTIDAMDTWEDLTELGYHLADLPVEPHLGKMVLCAVVLKCLDPILTIACTLAYRDPFVLPTQASQKRAAMLCRKRFTAGAFSDHMALLRAFQAWQKARSDGWERAFCEKNFLSQATMEIIIGMRTQLLGQLRASGFVRARGGGDIRDVNTNSENWAVVKAALVAGMYPNLVHVDRENLVLTGPKEKKVRFHPASVLSQPQYKKIPPANGQAAAIKALPTDWLIYDEMTRAHRIANIRCCSAVTPVTILVFCGPARLASNALQEPSSFRVDGIPNDSSDSEMEDKTTANLAALKLDEWLHFTLEPEAASLLLQLRQKWHSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD*"
     attR2           complement(4692..4705)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(4692..4706)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 4692..4769
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4708..4734
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      4735..4743
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4747..4769
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     4747..4769
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4770..4839
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 4774..4796
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4797..4816
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_difference complement(4817..4839)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4840..4843
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(4844..4866)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4844..4866)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 4844..4875
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           4867..4891
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 4876..4909
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4901..5035
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 4910..5065
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 5066..7776
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(5307..5341)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5353..5368)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(5577..5596)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     5698..5720
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(5759..5777)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5845..5949
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5950..6810
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(6168..6187)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      6981..7569
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     7470..7489
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     7723..7740
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 7777..7865
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 7866..10734
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    7946..7967
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        7982..8012
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    8020..8036
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     8044..8060
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   8086..8148
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    8324..8405
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     8432..8451
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(8522..9121)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     9007..9026
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(9122..9178)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(9188..9931)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(9329..9348)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     9574..9593
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(9932..9955)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(9955..9964)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(9984..10195)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(10196..10575)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     10724..10734
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 10735..10767
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(10744..10762)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GAGCCGACCA TCTTCCGTAT CTCCCAGACA
      421 ACCTGCACCC GGCGGAGGAG GCGGGGGGGG ACCATCACCT TGCGGGCCAG GTGGAGGAGG
      481 GCGAGCTAAA GGTCTTAAAG ATATCCGAAT CGACGAAGAA GTAAAGATTG CTGTAAACAT
      541 AGCACTTGAA CGTTTTCGCT ATGGCGATCA ACGTGAGATG GAGTTCCCCA GCTCCCTGAC
      601 ATCCACGGAG CGTGCGTTCA TACACCGGTT ATCCCAAAGC CTGGGACTGG TGAGCAAGTC
      661 CAAGGGGAAA GGGGCGAACC GTTATCTGAC AGTCAAGAAG AAGGACGGCA GCGAGACCGC
      721 CCACGCTATG ATGACTTGCA ACCTGACACA CAACACCAAG CACGCCGTAC GCTCTCTGAT
      781 CCAGCGGTTC CCCGTGACTA ACAAGGAAAG GACTGAGTTA TTACCAAAGA CCGAGCGAGG
      841 CAACGTTTTC GCCGTCGAGG CGGAGAATAG GGAGATGTCT AAAACGTCCG GACGCCTTAA
      901 TAACGGTATC CCACAAATCC CTGTCAAGAG GGGTGAGTCT GAGTTCGACA GCTTCCGGCA
      961 ATCCCTGCCC GTTTTCGAAA AGCAAGAGGA GATCGTGAAG ATTATCAAAG AGAACAAGGT
     1021 CGTCCTGATC GTCGGTGAGA CCGGTTCCGG GAAAACTACC CAAATCCCAC AATTTCTGCT
     1081 GGACGACTGT TTCAAGAACG GCATTCCTTG TAGAATTTTC TGCACACAGC CTCGGCGCCT
     1141 CGCCGCGATT GCAGTTGCAG AGCGCGTAGC TGCTGAACGA AGAGAGAGAA TCGGGCAGAC
     1201 CATAGGGTAC CAAATACGGC TCGAGAGTCG GGTGTCACCC AAAACTCTGC TCACCTTCTG
     1261 CACAAACGGA GTTCTGCTCC GCACTCTTAT GGCTGGGGAC TCTACCCTCT CTACAGTCAC
     1321 TCACGTCATA GTTGACGAGG TTCACGAGAG AGACAGGTTC TCTGACTTCC TGTTAACGAA
     1381 ACTCCGCGAC CTGCTCCAGA AACATCCTAC CCTGAAGCTG ATCCTGTCAT CTGCCGCGCT
     1441 GGACGTGAAC CTGTTCATTC GCTACTTCGG TAGCTGCCCT GTAATTTACA TTCAAGGTCG
     1501 ACCCTTCGAG GTTAAGGAAA TGTTCCTCGA GGACATCCTT CGCACCACAG GTTACACGAA
     1561 TAAGGAAATG CTGAAGTACA AGAAAGAGAA GCAACAAGAG GAAAAGCAGC AGACTACCTT
     1621 GACTGAGTGG TATAGCGCCC AGGAGAACTC TTTTAAACCG GAGAGCCAAA GACAACGCAC
     1681 AGTGTTAAAC GTCACCGACG AATACGATCT CTTAGACGAC GGCGGCGACG CCGTTTTCTC
     1741 TCAATTGACA GAGAAGGACG TCAACTGTCT GGAGCCCTGG CTGATCAAAG AGATGGACGC
     1801 CTGTCTGAGC GACATTTGGC TGCACAAGGA CATAGACGCT TTCGCCCAAG TGTTCCACCT
     1861 GATCCTGACC GAGAACGTGT CCGTGGACTA TAGGCACTCC GAGACATCTG CCACTGCGCT
     1921 TATGGTGGCG GCCGGCAGGG GCTTCGCCTC TCAGGTCGAG CAACTGATTT CTATGGGCGC
     1981 AAACGTGCAC AGCAAGGCCT CTAACGGGTG GATGGCCCTG GACTGGGCCA AGCATTTCGG
     2041 ACAAACCGAG ATCGTTGACC TGCTGGAGTC ATATTCTGCG ACCCTCGAGT TCGGCAACCT
     2101 CGACGAGTCC TCATTGGTGC AGACCAACGG GTCTGATCTG AGCGCAGAGG ATCGAGAACT
     2161 GCTCAAGGCA TACCACCACT CCTTTGACGA CGAGAAGGTT GATCTGGACC TTATTATGCA
     2221 CCTGCTGTAT AACATATGTC ACTCCTGCGA CGCCGGCGCC GTGCTGATCT TCTTGCCAGG
     2281 TTACGATGAG ATCGTGGGTT TGAGGGACCG GATTCTCTTC GACGATAAAC GCTTCGCAGA
     2341 TTCCACCCAC CGTTATCAGG TGTTCATGCT GCACTCCAAC ATGCAGACCA GCGACCAGAA
     2401 GAAGGTGCTG AAGAATCCGC CCGCCGGCGT ACGCAAGATT ATCCTCAGCA CAAACATCGC
     2461 GGAGACGTCT ATTACTGTGA ACGACGTGGT GTTCGTGATC GACTCCGGCA AAGTTAAGGA
     2521 AAAGTCTTTC GACGCACTCA ACTTCGTGAC CATGTTGAAG ATGGTGTGGA TCAGCAAGGC
     2581 ATCAGCAATT CAAAGGAAGG GAAGAGCGGG CAGATGCCGG CCCGGGATCT GCTTCCGACT
     2641 CTTTTCCCGC CTGAGATTTC AAAACATGCT CGAGTTCCAA ACACCAGAGC TGCTGCGAAT
     2701 GCCTCTGCAA GAGCTGTGTC TTCACACTAA ACTTCTCGCG CCCGTGAACT GCCCTATAGC
     2761 CGACTTCTTG ATGAAGGCCC CAGAGCCGCC CCCTGCACTG ATAGTGCGGA ACGCCGTGCA
     2821 GATGCTGAAA ACTATCGACG CTATGGACAC TTGGGAAGAC CTCACGGAGT TGGGTTACCA
     2881 CCTGGCCGAT CTTCCCGTGG AGCCTCACCT GGGCAAGATG GTACTGTGCG CCGTCGTCCT
     2941 TAAATGCTTG GATCCTATTC TCACCATCGC CTGTACCCTT GCATACAGAG ACCCCTTCGT
     3001 GCTCCCGACA CAAGCTAGCC AGAAGCGCGC CGCAATGCTC TGCCGCAAGA GATTCACCGC
     3061 CGGCGCCTTT TCCGATCACA TGGCACTCCT GAGGGCCTTT CAAGCGTGGC AGAAGGCTAG
     3121 GAGCGACGGA TGGGAAAGAG CTTTCTGCGA GAAGAACTTC CTGAGTCAAG CAACGATGGA
     3181 GATTATAATT GGTATGCGGA CGCAACTGCT GGGACAGCTG CGGGCGAGTG GATTCGTCAG
     3241 GGCCAGGGGC GGCGGGGATA TAAGAGATGT GAATACGAAT TCCGAAAACT GGGCGGTGGT
     3301 GAAGGCAGCT CTGGTCGCCG GGATGTACCC CAACCTCGTT CATGTTGATC GCGAAAACTT
     3361 GGTCCTGACG GGTCCCAAAG AAAAGAAGGT CAGGTTCCAC CCCGCCTCTG TGCTGAGCCA
     3421 ACCCCAGTAC AAGAAAATCC CACCCGCAAA CGGGCAGGCC GCCGCCATCA AAGCTTTGCC
     3481 GACCGACTGG CTGATCTACG ACGAGATGAC TAGGGCACAC CGCATCGCAA ACATCCGCTG
     3541 CTGCTCTGCT GTTACCCCGG TGACCATTCT GGTCTTTTGC GGGCCTGCCC GGCTGGCCTC
     3601 TAACGCCCTG CAAGAGCCCA GCAGCTTCCG CGTTGACGGT ATCCCTAACG ATTCTTCTGA
     3661 CAGCGAGATG GAAGATAAGA CCACCGCGAA CTTAGCTGCG CTCAAGTTGG ACGAATGGTT
     3721 ACACTTTACT CTCGAACCTG AAGCGGCCTC TTTGCTTCTC CAACTGAGGC AGAAATGGCA
     3781 CTCTTTGTTC TTGCGGCGCA TGCGGGCCCC TTCAAAGCCC TGGAGCCAGG TGGACGAGGC
     3841 AACTATTCGT GCCATCATTG CCGTGCTGTC TACAGAGGAG CAATCCGCCG GGCTCCAGCA
     3901 GCCATCCGGA ATAGGACAGC GGCCGCGACC CATGAGCAGC GAGGAATTGC CGCTGGCTTC
     3961 CTCATGGCGG TCCAACAACT CTCGCAAGTC TAGTGCCGAC ACCGAGTTCA GCGACGAATG
     4021 CACGACCGCC GAGAGGGTCC TTATGAAGTC CCCCTCACCC GCCCTGCATC CCCCGCAGAA
     4081 ATATAAGGAC AGGGGCATAC TCCACCCCAA GCGCGGGACC GAAGATAGGT CTGACCAATC
     4141 TTCCCTCAAG AGCACTGATA GTTCCTCTTA TCCGTCACCG TGCGCATCCC CCAGCCCGCC
     4201 CTCTTCTGGC AAAGGAAGCA AGAGCCCAAG TCCCAGGCCC AATATGCCAG TGAGATATTT
     4261 TATCATGAAA TCTTCCAACT TAAGGAATCT CGAGATCAGC CAGCAAAAGG GAATTTGGAG
     4321 CACCACCCCA TCCAACGAGA GGAAATTGAA CAGAGCATTC TGGGAGTCTT CAATCGTGTA
     4381 TCTGGTCTTC AGTGTGCAGG GCAGTGGGCA CTTTCAAGGG TTCTCCCGCA TGTCCAGTGA
     4441 AATCGGGAGA GAGAAATCCC AAGATTGGGG TTCAGCCGGG TTGGGCGGTG TGTTCAAAGT
     4501 TGAATGGATC CGTAAAGAGT CATTGCCTTT CCAGTTCGCT CATCACCTGC TGAACCCCTG
     4561 GAACGATAAT AAGAAGGTCC AAATTTCACG CGACGGCCAA GAGTTAGAGC CATTGGTCGG
     4621 GGAGCAACTT TTGCAACTGT GGGAGCGGCT CCCGCTGGGC GAGAAGAATA CCACCGACTA
     4681 GACCCAGCTT TCTTGTACAA AGTGGTATAC CCATACGATG TTCCAGATTA CGCTTAGTAA
     4741 TGAGAAATAC TCAGAAGATG TCACTCACCA TAGTCCGCAG TGAAAGATAA ATGATCTGCC
     4801 TATTATAGGG GTGCCGGTTT TAGAGCTAGA AATAGCAAGT AGGCCACTGA GCAAGAACTA
     4861 TGAATGAGAT CTTTAGACAT ATTGCGGCCG CTTTCTCCGC GATCCAGACA TGATAAGATA
     4921 CATTGATGAG TTTGGACAAA CCAAAACTAG AATGCAGTGA AAAAAATGCC TTATTTGTGA
     4981 AATTTGTGAT GCTATTGCCT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTATCTA
     5041 TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA
     5101 ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT
     5161 CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG
     5221 GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG
     5281 AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG
     5341 GTTAAGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC
     5401 CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC
     5461 CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG
     5521 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA
     5581 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG
     5641 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CATGTCTCCG
     5701 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACACGCGAGT TCGAAAGGGC
     5761 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA
     5821 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT
     5881 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
     5941 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
     6001 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
     6061 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
     6121 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
     6181 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
     6241 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
     6301 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
     6361 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA
     6421 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
     6481 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
     6541 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
     6601 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
     6661 CGTGGTTCAC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
     6721 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
     6781 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
     6841 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     6901 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     6961 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     7021 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     7081 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG
     7141 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     7201 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     7261 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     7321 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA
     7381 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     7441 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     7501 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     7561 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     7621 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     7681 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     7741 GAAGCGGATG AGCGCCCAAT ACGCAAACCG CCTCTCACCG CGCGTTGGCC GATTCATTAA
     7801 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAAACCGC
     7861 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
     7921 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG
     7981 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC
     8041 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGGTCGA CTTAACCCTA GAAAGATAAT
     8101 CATATTGTGA CGTACGTTAA AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT
     8161 CTGTATATCG AGGTTTATTT ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT
     8221 TACATACTAA TAATAAATTC AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA
     8281 AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT TTAGCAGTGA AAAAAATGCT
     8341 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC
     8401 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG
     8461 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT CCTCTGGAGA
     8521 TTCATGCGCC AGGTTTTCTG GTCATGCACC ACGTTCGTGG GCCCTCAGGA ACCTCCACGT
     8581 CAGCGGTAAC TGTGAAGCCC AGTCGCTCGT AAAAAGGAAG ATTCCGGGGA GCACTGGTTT
     8641 CGAGGAACGC CGGCACGCCA GCCCGTTCCG CGGCCTCAAC ACCAGGAAGA ACGACAGCGC
     8701 TTCCCAAACC TTTACCTTGG TGATCTGGGC TGACACCGAC AGTGGCAAGA AACCACGCAG
     8761 GCTCCTTTGG TCTATGAGGA GCCAGCAAGC CCTCCATTTG CTGTTGTGCC GCCAACCTAC
     8821 TCCCTGACAG CTCGGCCATC CGCGGACCTA TTTCCGCGAA CACTGCGCCG GCTTCGACGC
     8881 TTTCCGGAGT AGTCCAGACC GCAACTGCGG CACCATCGTC CGCAACCCAA ACTTTACCAA
     8941 TGTCCAGACC CACGCGCGTA AGAAACAGCT CTTGCAACTC AGTTACCCTC TCTATGTGTC
     9001 TGTCCGGATC TACGGTATGG CGGGTCGCTG GATAATCTGC AAATGCTGCG GCAAGTGTTC
     9061 GGACAGCCCG TGGTACATCG TCACGGGTAG CGAGCCTAAC AGTGGGCTTA TATTCCGTCA
     9121 TGGGGCCGGG GTTCTCCTCC ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG TTGGTGGCGC
     9181 CGCTGCCCCC GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT
     9241 CAAGGTCAAA GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG
     9301 CGTCGGTCGG CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT
     9361 CCAGGCCGAA AAGGAAGGCA GGTTCGGCTC CCTGCCGGTC GAACAGCTCA ATTGCTTGTT
     9421 TCAGAAGTGG GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT
     9481 GCTCCTGTTC CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA
     9541 GTGCGTTCTC CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT
     9601 CGTACTGTTT CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCA CGATGTGAGA
     9661 GGAGAGCACA GCGGTATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT
     9721 CCAGGGGGCA GGAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGGCAGG GCATCGAGCA
     9781 GGGCCCGCTT GTTCTTCACG TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG
     9841 CGAGTTTCCT TGTCGTCAGG CCTTCGATAC CGACTCCATT GAGTAATTCC AGAGCAGAGT
     9901 TTATGACTTT GCTCTTGTCC AGTCTAGACA TCTTATCGTC ATCGTCTTTG TAATCCATGG
     9961 TGGCGGATCC CGCGTCACGA CACCTGTGTT CTGGCGGCAA ACCCGTTGCG AAAAAGAACG
    10021 TTCACGGCGA CTACTGCACT TATATACGGT TCTCCCCCAC CCTCGGGAAA AAGGCGGAGC
    10081 CAGTACACGA CATCACTTTC CCAGTTTACC CCGCGCCACC TTCTCTAGGC ACCGGTTCAA
    10141 TTGCCGACCC CTCCCCCCAA CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC TGCCCCATGG
    10201 TAATAGCCAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTGCCAT AAGGTCATGT
    10261 ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG
    10321 GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT
    10381 GACGTCAATG GAAAGTGCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA
    10441 ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC
    10501 GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAG
    10561 TCAATAATCA ATGTCATAAG CTCTAATCGC ATAATCGAGC TAAGAGCGAT TAATATGCTT
    10621 AGCTAGCTTA GCTAAGCTAG ATTTCTCTAT TCTCCCACAA TATATAGCAG CTATTAGCTT
    10681 AGCAGTCCGA ATACGATCGT CCACGATCCA TAAGCGTAAC ACATTTGAGT AGCGACTGAC
    10741 GGGCACCGGA GCCACTCGAG TGGAATT
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