LOCUS       CK530_TF132_NELFE       7607 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1532..1545)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1532..1546)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1532..1609
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1548..1574
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1575..1583
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1587..1609
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1587..1609
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1610..1679
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1614..1636
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1637..1656
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference complement(1657..1679)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1680..1683
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1684..1706)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1684..1706)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 1684..1715
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1707..1731
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1716..1749
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    1741..1875
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1750..1905
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1906..4616
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2147..2181)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2193..2208)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(2417..2436)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2538..2560
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2599..2617)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2685..2789
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2790..3650
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3008..3027)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      3821..4409
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4310..4329
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4563..4580
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4617..4705
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4706..7574
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4786..4807
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4822..4852
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4860..4876
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4884..4900
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4926..4988
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5164..5245
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5272..5291
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5362..5961)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5847..5866
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5962..6018)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6028..6771)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6169..6188)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6414..6433
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(6772..6795)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6795..6804)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6824..7035)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7036..7415)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7564..7574
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7575..7607
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7584..7602)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GCTGGTCATT CCACCTGGTT TATCTGAAGA
      421 GGAAGAAGCT CTCCAAAAGA AGTTTAATAA ATTAAAGAAG AAGAAGAAAG CCCTGCTCGC
      481 CCTCAAGAAA CAGTCATCTT CTTCAACTAC TTCCCAGGGC GGCGTTAAGA GGTCTTTAAG
      541 CGAACAACCA GTAATGGATA CCGCTACGGC CACTGAACAA GCGAAACAAC TTGTAAAATC
      601 CGGTGCTATT TCAGCAATAA AAGCGGAAAC TAAGAATAGT GGTTTTAAAA GAAGCAGGAC
      661 ACTGGAAGGA AAACTGAAAG ATCCAGAAAA GGGGCCCGTA CCTACCTTTC AACCTTTTCA
      721 ACGCTCTATT AGCGCCGACG ACGATTTGCA GGAAAGCAGC CGCCGGCCAC AACGAAAGTC
      781 ACTTTACGAA AGTTTCGTCA GTTCCAGCGA CCGCTTACGG GAGCTGGGGC CGGATGGCGA
      841 GGAAGCTGAA GGACCTGGCG CTGGCGACGG ACCACCCAGA TCATTCGATT GGGGTTACGA
      901 GGAAAGGTCC GGTGCTCATA GCTCTGCAAG TCCACCGCGT TCAAGGTCCC GTGATCGGTC
      961 TCACGAAAGA AATAGGGACA GGGACCGCGA TCGCGAACGA GACCGCGATA GGGACAGAGA
     1021 TCGGGATCGG GAACGTGATC GCGACCGTGA CCGCGACAGG GACAGGGATA GAGAGAGGGA
     1081 TCGGGACCGA GAACGAGACC GGGATAGGGA TCGCGAAGGA CCGTTTCGGC GGTCCGACAG
     1141 TTTTCCGGAG AGAAGGGCGC CCCGAAAGGG AAACACCTTG TACGTCTACG GGGAAGATAT
     1201 GACCCCTACT CTGCTTCGAG GAGCGTTTTC CCCCTTCGGG AATATTATCG ATTTAAGCAT
     1261 GGATCCGCCA CGCAATTGCG CTTTTGTGAC ATACGAGAAA ATGGAATCTG CTGACCAAGC
     1321 TGTGGCCGAA CTTAATGGCA CACAAGTAGA AAGCGTGCAA CTGAAGGTGA ATATTGCAAG
     1381 AAAGCAACCT ATGTTGGACG CGGCCACAGG GAAAAGCGTG TGGGGATCTT TGGCCGTGCA
     1441 AAATTCCCCA AAAGGGTGTC ATCGTGATAA ACGTACACAA ATCGTTTATA GCGACGATGT
     1501 TTATAAAGAG AATCTGGTCG ACGGTTTCTA ACTTGTACAA AGTGGTATAC CCATACGATG
     1561 TTCCAGATTA CGCTTAGTAA TGAGAAATAC TCAGAAGATG TCACTCACCA TAGTCCGCAG
     1621 TGAAAGATAA ATGATCTGAG CAATATACAA AAGACAGTTT TAGAGCTAGA AATAGCAAGT
     1681 AGGCCACTGA GCAAGAACTA TGAATGAGAT CTTTAGACAT ATTGCGGCCG CTTTCTCCGC
     1741 GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCAAAACTAG AATGCAGTGA
     1801 AAAAAATGCC TTATTTGTGA AATTTGTGAT GCTATTGCCT TATTTGTAAC CATTATAAGC
     1861 TGCAATAAAC AAGTTATCTA TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA
     1921 GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT
     1981 AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC
     2041 ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC
     2101 GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT
     2161 ACGCAGACTA TCTTTCTAGG GTTAAGAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT
     2221 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT
     2281 GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG
     2341 GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
     2401 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC
     2461 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC
     2521 AAGCTGTGAC CATGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA
     2581 ACACGCGAGT TCGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA
     2641 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT
     2701 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA
     2761 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA
     2821 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG
     2881 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA
     2941 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA
     3001 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC
     3061 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC
     3121 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA
     3181 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC
     3241 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA
     3301 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC
     3361 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG
     3421 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG
     3481 ATAAATCTGG AGCCGGTGAG CGTGGTTCAC GCGGTATCAT TGCAGCACTG GGGCCAGATG
     3541 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC
     3601 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC
     3661 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT
     3721 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC
     3781 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC
     3841 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
     3901 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA
     3961 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC
     4021 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT
     4081 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA
     4141 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
     4201 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC
     4261 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT
     4321 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT
     4381 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
     4441 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
     4501 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
     4561 GCAGCGAGTC AGTGAGCGAG GAAGCGGATG AGCGCCCAAT ACGCAAACCG CCTCTCACCG
     4621 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA
     4681 GTGAGCGCAA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
     4741 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
     4801 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
     4861 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGGTCGA
     4921 CTTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA AGATAATCAT GCGTAAAATT
     4981 GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT ATTAATTTGA ATAGATATTA
     5041 AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC AACAAACAAT TTATTTATGT
     5101 TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT TCTATAAAGT AACAAAACTT
     5161 TTAGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC
     5221 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
     5281 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
     5341 TGATTATGAT CCTCTGGAGA TTCATGCGCC AGGTTTTCTG GTCATGCACC ACGTTCGTGG
     5401 GCCCTCAGGA ACCTCCACGT CAGCGGTAAC TGTGAAGCCC AGTCGCTCGT AAAAAGGAAG
     5461 ATTCCGGGGA GCACTGGTTT CGAGGAACGC CGGCACGCCA GCCCGTTCCG CGGCCTCAAC
     5521 ACCAGGAAGA ACGACAGCGC TTCCCAAACC TTTACCTTGG TGATCTGGGC TGACACCGAC
     5581 AGTGGCAAGA AACCACGCAG GCTCCTTTGG TCTATGAGGA GCCAGCAAGC CCTCCATTTG
     5641 CTGTTGTGCC GCCAACCTAC TCCCTGACAG CTCGGCCATC CGCGGACCTA TTTCCGCGAA
     5701 CACTGCGCCG GCTTCGACGC TTTCCGGAGT AGTCCAGACC GCAACTGCGG CACCATCGTC
     5761 CGCAACCCAA ACTTTACCAA TGTCCAGACC CACGCGCGTA AGAAACAGCT CTTGCAACTC
     5821 AGTTACCCTC TCTATGTGTC TGTCCGGATC TACGGTATGG CGGGTCGCTG GATAATCTGC
     5881 AAATGCTGCG GCAAGTGTTC GGACAGCCCG TGGTACATCG TCACGGGTAG CGAGCCTAAC
     5941 AGTGGGCTTA TATTCCGTCA TGGGGCCGGG GTTCTCCTCC ACGTCGCCGG CCTGCTTCAG
     6001 CAGGCTGAAG TTGGTGGCGC CGCTGCCCCC GGGGAGCATG TCAAGGTCAA AATCGTCAAG
     6061 AGCGTCAGCA GGCAGCATAT CAAGGTCAAA GTCGTCAAGG GCATCGGCTG GGAGCATGTC
     6121 TAAGTCAAAA TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT TCGCACTTTA GCTGTTTCTC
     6181 CAGGCCACAT ATGATTAGTT CCAGGCCGAA AAGGAAGGCA GGTTCGGCTC CCTGCCGGTC
     6241 GAACAGCTCA ATTGCTTGTT TCAGAAGTGG GGGCATAGAA TCGGTGGTAG GTGTCTCTCT
     6301 TTCCTCTTTT GCTACTTGAT GCTCCTGTTC CTCCAATACG CAGCCCAGTG TAAAGTGGCC
     6361 CACGGCGGAC AGAGCGTACA GTGCGTTCTC CAGGGAGAAG CCTTGCTGAC ACAGGAACGC
     6421 GAGCTGATTT TCCAGGGTTT CGTACTGTTT CTCTGTTGGG CGGGTGCCGA GATGCACTTT
     6481 AGCCCCGTCA CGATGTGAGA GGAGAGCACA GCGGTATGAC TTGGCGTTGT TCCGCAGAAA
     6541 GTCTTGCCAT GACTCGCCTT CCAGGGGGCA GGAGTGGGTA TGATGCCTGT CCAGCATCTC
     6601 GATTGGCAGG GCATCGAGCA GGGCCCGCTT GTTCTTCACG TGCCAGTACA GGGTAGGCTG
     6661 CTCAACTCCC AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG CCTTCGATAC CGACTCCATT
     6721 GAGTAATTCC AGAGCAGAGT TTATGACTTT GCTCTTGTCC AGTCTAGACA TCTTATCGTC
     6781 ATCGTCTTTG TAATCCATGG TGGCGGATCC CGCGTCACGA CACCTGTGTT CTGGCGGCAA
     6841 ACCCGTTGCG AAAAAGAACG TTCACGGCGA CTACTGCACT TATATACGGT TCTCCCCCAC
     6901 CCTCGGGAAA AAGGCGGAGC CAGTACACGA CATCACTTTC CCAGTTTACC CCGCGCCACC
     6961 TTCTCTAGGC ACCGGTTCAA TTGCCGACCC CTCCCCCCAA CTTCTCGGGC ACTGTGGGCC
     7021 ATGTGCGCTC TGCCCCATGG TAATAGCCAT GACTAATACG TAGATGTACT GCCAAGTAGG
     7081 AAAGTGCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC
     7141 GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA
     7201 CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTGCCT ATTGGCGTTA CTATGGGAAC
     7261 ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT
     7321 TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT
     7381 TGATTACTAT TAATAACTAG TCAATAATCA ATGTCATAAG CTCTAATCGC ATAATCGAGC
     7441 TAAGAGCGAT TAATATGCTT AGCTAGCTTA GCTAAGCTAG ATTTCTCTAT TCTCCCACAA
     7501 TATATAGCAG CTATTAGCTT AGCAGTCCGA ATACGATCGT CCACGATCCA TAAGCGTAAC
     7561 ACATTTGAGT AGCGACTGAC GGGCACCGGA GCCACTCGAG TGGAATT
//