LOCUS       CK530_TF134_NFYB        7088 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            concatenation starting/ending at bases [CTTGTACAAAGTG] 3' end
            ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA] Insert 5' end ligation method:
            concatenation from start/end of sequence 3' end ligation method:
            concatenation from start/end of sequence Backbone 5' end ligation
            method: concatenation starting/ending at bases [CTTGTACAAAGTG] 3'
            end ligation method: concatenation starting/ending at bases
            [GATCCAGCCTACCACAAGTTTGTACAAA]
FEATURES             Location/Qualifiers
     extracted re... 1..382
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..382
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..382
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1013..1026)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1013..1027)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1013..1090
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1029..1055
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1056..1064
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1068..1090
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     1068..1090
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 1091..1160
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1095..1117
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1118..1137
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference complement(1138..1160)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1161..1164
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1165..1187)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1165..1187)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 1165..1196
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1188..1212
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1197..1230
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    1222..1356
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1231..1386
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1387..4097
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(1628..1662)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(1674..1689)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(1898..1917)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2019..2041
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2080..2098)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2166..2270
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2271..3131
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2489..2508)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      3302..3890
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     3791..3810
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4044..4061
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4098..4186
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4187..7055
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4267..4288
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4303..4333
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4341..4357
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4365..4381
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4407..4469
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4645..4726
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     4753..4772
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(4843..5442)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5328..5347
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5443..5499)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5509..6252)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(5650..5669)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     5895..5914
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(6253..6276)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6276..6285)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6305..6516)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(6517..6896)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7045..7055
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7056..7088
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7065..7083)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAGCCACCAT GACAATGGAT GGTGACAGTT CTACAACAGA
      421 TGCTTCTCAA CTAGGAATCT CTGCAGACTA TATTGGAGGA AGTCATTATG TTATACAGCC
      481 TCATGATGAT ACTGAGGACA GCATGAATGA TCATGAAGAC ACAAATGGTT CAAAAGAAAG
      541 TTTCAGAGAA CAAGATATAT ATCTTCCAAT AGCAAACGTG GCTAGGATAA TGAAAAATGC
      601 CATACCTCAA ACGGGAAAGA TTGCAAAAGA TGCCAAAGAA TGTGTTCAAG AATGTGTAAG
      661 TGAGTTCATC AGTTTTATAA CATCTGAAGC AAGTGAAAGG TGCCATCAAG AGAAACGGAA
      721 AACAATCAAT GGAGAAGATA TTCTCTTTGC TATGTCTACT TTAGGCTTTG ACAGTTATGT
      781 GGAACCTCTG AAATTATACC TTCAGAAATT CAGAGAGGCT ATGAAAGGAG AAAAGGGAAT
      841 TGGTGGAGCA GTCACAGCTA CAGATGGACT AAGTGAAGAG CTTACAGAGG AGGCATTTAC
      901 TAACCAGTTA CCAGCTGGCT TAATAACCAC AGACGGTCAA CAACAAAATG TTATGGTTTA
      961 CACAACATCA TATCAACAGA TTTCTGGTGT TCAGCAAATT CAGTTTTCAT GACTTGTACA
     1021 AAGTGGTATA CCCATACGAT GTTCCAGATT ACGCTTAGTA ATGAGAAATA CTCAGAAGAT
     1081 GTCACTCACC ATAGTCCGCA GTGAAAGATA AATGATCGAC TGTAACTGCG ATTGCAAGTT
     1141 TTAGAGCTAG AAATAGCAAG TAGGCCACTG AGCAAGAACT ATGAATGAGA TCTTTAGACA
     1201 TATTGCGGCC GCTTTCTCCG CGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA
     1261 ACCAAAACTA GAATGCAGTG AAAAAAATGC CTTATTTGTG AAATTTGTGA TGCTATTGCC
     1321 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTATCT ATAACAAGAA AATATATATA
     1381 TAATAAGTTA TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA TGAGTTAAAT
     1441 CTTAAAAGTC ACGTAAAAGA TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC
     1501 AAAATCAGTG ACACTTACCG CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT
     1561 GAGATGTCCT AAATGCACAG CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC
     1621 AAGAATGCAT GCGTCAATTT TACGCAGACT ATCTTTCTAG GGTTAAGAAT TCACTGGCCG
     1681 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG
     1741 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC
     1801 AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC
     1861 TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT
     1921 AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
     1981 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCATGTCTCC GGGAGCTGCA TGTGTCAGAG
     2041 GTTTTCACCG TCATCACCGA AACACGCGAG TTCGAAAGGG CCTCGTGATA CGCCTATTTT
     2101 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA
     2161 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     2221 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
     2281 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
     2341 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
     2401 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
     2461 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG
     2521 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
     2581 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
     2641 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
     2701 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
     2761 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
     2821 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
     2881 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
     2941 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCA CGCGGTATCA
     3001 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
     3061 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
     3121 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
     3181 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
     3241 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     3301 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     3361 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     3421 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     3481 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     3541 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     3601 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     3661 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
     3721 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     3781 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     3841 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     3901 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
     3961 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
     4021 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAT GAGCGCCCAA
     4081 TACGCAAACC GCCTCTCACC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
     4141 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAAACCG CCTCTCCCCG CGCGTTGGCC
     4201 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA
     4261 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
     4321 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA
     4381 CCATGATTAC GCCAAGGTCG ACTTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA
     4441 AAGATAATCA TGCGTAAAAT TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT
     4501 TATTAATTTG AATAGATATT AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT
     4561 CAACAAACAA TTTATTTATG TTTATTTATT TATTAAAAAA AAACAAAAAC TCAAAATTTC
     4621 TTCTATAAAG TAACAAAACT TTTAGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA
     4681 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG
     4741 CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA
     4801 CCTCTACAAA TGTGGTATGG CTGATTATGA TCCTCTGGAG ATTCATGCGC CAGGTTTTCT
     4861 GGTCATGCAC CACGTTCGTG GGCCCTCAGG AACCTCCACG TCAGCGGTAA CTGTGAAGCC
     4921 CAGTCGCTCG TAAAAAGGAA GATTCCGGGG AGCACTGGTT TCGAGGAACG CCGGCACGCC
     4981 AGCCCGTTCC GCGGCCTCAA CACCAGGAAG AACGACAGCG CTTCCCAAAC CTTTACCTTG
     5041 GTGATCTGGG CTGACACCGA CAGTGGCAAG AAACCACGCA GGCTCCTTTG GTCTATGAGG
     5101 AGCCAGCAAG CCCTCCATTT GCTGTTGTGC CGCCAACCTA CTCCCTGACA GCTCGGCCAT
     5161 CCGCGGACCT ATTTCCGCGA ACACTGCGCC GGCTTCGACG CTTTCCGGAG TAGTCCAGAC
     5221 CGCAACTGCG GCACCATCGT CCGCAACCCA AACTTTACCA ATGTCCAGAC CCACGCGCGT
     5281 AAGAAACAGC TCTTGCAACT CAGTTACCCT CTCTATGTGT CTGTCCGGAT CTACGGTATG
     5341 GCGGGTCGCT GGATAATCTG CAAATGCTGC GGCAAGTGTT CGGACAGCCC GTGGTACATC
     5401 GTCACGGGTA GCGAGCCTAA CAGTGGGCTT ATATTCCGTC ATGGGGCCGG GGTTCTCCTC
     5461 CACGTCGCCG GCCTGCTTCA GCAGGCTGAA GTTGGTGGCG CCGCTGCCCC CGGGGAGCAT
     5521 GTCAAGGTCA AAATCGTCAA GAGCGTCAGC AGGCAGCATA TCAAGGTCAA AGTCGTCAAG
     5581 GGCATCGGCT GGGAGCATGT CTAAGTCAAA ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT
     5641 TTCGCACTTT AGCTGTTTCT CCAGGCCACA TATGATTAGT TCCAGGCCGA AAAGGAAGGC
     5701 AGGTTCGGCT CCCTGCCGGT CGAACAGCTC AATTGCTTGT TTCAGAAGTG GGGGCATAGA
     5761 ATCGGTGGTA GGTGTCTCTC TTTCCTCTTT TGCTACTTGA TGCTCCTGTT CCTCCAATAC
     5821 GCAGCCCAGT GTAAAGTGGC CCACGGCGGA CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA
     5881 GCCTTGCTGA CACAGGAACG CGAGCTGATT TTCCAGGGTT TCGTACTGTT TCTCTGTTGG
     5941 GCGGGTGCCG AGATGCACTT TAGCCCCGTC ACGATGTGAG AGGAGAGCAC AGCGGTATGA
     6001 CTTGGCGTTG TTCCGCAGAA AGTCTTGCCA TGACTCGCCT TCCAGGGGGC AGGAGTGGGT
     6061 ATGATGCCTG TCCAGCATCT CGATTGGCAG GGCATCGAGC AGGGCCCGCT TGTTCTTCAC
     6121 GTGCCAGTAC AGGGTAGGCT GCTCAACTCC CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG
     6181 GCCTTCGATA CCGACTCCAT TGAGTAATTC CAGAGCAGAG TTTATGACTT TGCTCTTGTC
     6241 CAGTCTAGAC ATCTTATCGT CATCGTCTTT GTAATCCATG GTGGCGGATC CCGCGTCACG
     6301 ACACCTGTGT TCTGGCGGCA AACCCGTTGC GAAAAAGAAC GTTCACGGCG ACTACTGCAC
     6361 TTATATACGG TTCTCCCCCA CCCTCGGGAA AAAGGCGGAG CCAGTACACG ACATCACTTT
     6421 CCCAGTTTAC CCCGCGCCAC CTTCTCTAGG CACCGGTTCA ATTGCCGACC CCTCCCCCCA
     6481 ACTTCTCGGG CACTGTGGGC CATGTGCGCT CTGCCCCATG GTAATAGCCA TGACTAATAC
     6541 GTAGATGTAC TGCCAAGTAG GAAAGTGCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC
     6601 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG
     6661 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTGCC
     6721 TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG
     6781 CGGTCAGCCA GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT
     6841 ATGAACTAAT GACCCCGTAA TTGATTACTA TTAATAACTA GTCAATAATC AATGTCATAA
     6901 GCTCTAATCG CATAATCGAG CTAAGAGCGA TTAATATGCT TAGCTAGCTT AGCTAAGCTA
     6961 GATTTCTCTA TTCTCCCACA ATATATAGCA GCTATTAGCT TAGCAGTCCG AATACGATCG
     7021 TCCACGATCC ATAAGCGTAA CACATTTGAG TAGCGACTGA CGGGCACCGG AGCCACTCGA
     7081 GTGGAATT
//