LOCUS       CK530_TF139_RARG_(BW88) 7847 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases cttgtacaaa 3' end ligation method: homology
            with bases tttgtacaaa Insert 5' end ligation method: homology with
            bases tttgtacaaa 3' end ligation method: homology with bases
            cttgtacaaa Backbone 5' end ligation method: homology with bases
            cttgtacaaa 3' end ligation method: homology with bases tttgtacaaa
FEATURES             Location/Qualifiers
     primer_bind     complement(95..114)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      908..1496
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     1397..1416
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     1650..1667
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 1704..1792
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1793..4661
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    1873..1894
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        1909..1939
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    1947..1963
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     1971..1987
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   2013..2075
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    2251..2332
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     2359..2378
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(2449..3048)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     2934..2953
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(3049..3105)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(3115..3858)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(3256..3275)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     3501..3520
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(3859..3882)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(3882..3891)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(3911..4122)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(4123..4502)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4651..4661
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 4662..4694
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(4671..4689)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     extracted re... 4695..5076
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        4703..4958
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    4711..4729
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    4747..4765
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    4783..4801
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    4819..4837
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    4855..4873
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    4891..4909
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    4927..4945
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        4959..5058
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5062..5076
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           5063..5076
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    5067..5086
                     /label="attL1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5067..6475
                     /label="Insert Sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            5093..5276
                     /label="RARG-203 Exon 3"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             join(5093..5276,5277..5425,5426..5567,5568..5728,5729..5905,5906..6110,6111..6269,6270..6455)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MATNKERLFAAGALGPGSGYPGAGFPFAFPGALRGSPPFEMLSPSFRGLGQPDLPKEMASLSVETQSTSSEEMVPSSPSPPPPPRVYKPCFVCNDKSSGYHYGVSSCEGCKGFFRRSIQKNMVYTCHRDKNCIINKVTRNRCQYCRLQKCFEVGMSKEAVRNDRNKKKKEVKEEGSPDSYELSPQLEELITKVSKAHQETFPSLCQLGKYTTNSSADHRVQLDLGLWDKFSELATKCIIKIVEFAKRLPGFTGLSIADQITLLKAACLDILMLRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFAGQLLPLEMDDTETGLLSAICLICGDRMDLEEPEKVDKLQEPLLEALRLYARRRRPSQPYMFPRMLMKITDLRGISTKGAERAITLKMEIPGPMPPLIREMLENPEMFEDDSSQPGPHPNASSEDEVPGGQGKGGLKSPA"
     gene            5093..6455
                     /label="RARG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     exon            5277..5425
                     /label="RARG-203 Exon 4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            5426..5567
                     /label="RARG-203 Exon 5"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            5568..5728
                     /label="RARG-203 Exon 6"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     exon            5729..5905
                     /label="RARG-203 Exon 7"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            5906..6110
                     /label="RARG-203 Exon 8"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            6111..6269
                     /label="RARG-203 Exon 9"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     exon            6270..6455
                     /label="RARG-203 Exon 10"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    complement(6458..6475)
                     /label="attL2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     attR2           complement(6466..6479)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(6466..6480)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 6466..6543
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             6482..6508
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      6509..6517
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     6521..6543
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     6521..6543
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 6544..6613
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 6548..6570
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6571..6590
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference complement(6591..6613)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 6614..6617
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(6618..6640)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(6618..6640)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 6618..6649
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           6641..6665
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 6650..6683
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    6675..6809
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 6684..6839
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 6840..1703
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(7081..7115)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(7127..7142)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(7351..7370)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     7472..7494
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(7533..7551)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        7619..7723
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             7724..737
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
       61 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA
      121 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
      181 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
      241 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
      301 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
      361 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
      421 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
      481 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
      541 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGTTCACGCG
      601 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
      661 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
      721 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
      781 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA
      841 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
      901 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
      961 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     1021 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC
     1081 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     1141 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     1201 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     1261 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     1321 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     1381 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     1441 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     1501 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     1561 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     1621 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGATGAGC
     1681 GCCCAATACG CAAACCGCCT CTCACCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG
     1741 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AAACCGCCTC TCCCCGCGCG
     1801 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
     1861 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
     1921 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
     1981 CTATGACCAT GATTACGCCA AGGTCGACTT AACCCTAGAA AGATAATCAT ATTGTGACGT
     2041 ACGTTAAAGA TAATCATGCG TAAAATTGAC GCATGTGTTT TATCGGTCTG TATATCGAGG
     2101 TTTATTTATT AATTTGAATA GATATTAAGT TTTATTATAT TTACACTTAC ATACTAATAA
     2161 TAAATTCAAC AAACAATTTA TTTATGTTTA TTTATTTATT AAAAAAAAAC AAAAACTCAA
     2221 AATTTCTTCT ATAAAGTAAC AAAACTTTTA GCAGTGAAAA AAATGCTTTA TTTGTGAAAT
     2281 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA
     2341 CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA
     2401 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCCT CTGGAGATTC ATGCGCCAGG
     2461 TTTTCTGGTC ATGCACCACG TTCGTGGGCC CTCAGGAACC TCCACGTCAG CGGTAACTGT
     2521 GAAGCCCAGT CGCTCGTAAA AAGGAAGATT CCGGGGAGCA CTGGTTTCGA GGAACGCCGG
     2581 CACGCCAGCC CGTTCCGCGG CCTCAACACC AGGAAGAACG ACAGCGCTTC CCAAACCTTT
     2641 ACCTTGGTGA TCTGGGCTGA CACCGACAGT GGCAAGAAAC CACGCAGGCT CCTTTGGTCT
     2701 ATGAGGAGCC AGCAAGCCCT CCATTTGCTG TTGTGCCGCC AACCTACTCC CTGACAGCTC
     2761 GGCCATCCGC GGACCTATTT CCGCGAACAC TGCGCCGGCT TCGACGCTTT CCGGAGTAGT
     2821 CCAGACCGCA ACTGCGGCAC CATCGTCCGC AACCCAAACT TTACCAATGT CCAGACCCAC
     2881 GCGCGTAAGA AACAGCTCTT GCAACTCAGT TACCCTCTCT ATGTGTCTGT CCGGATCTAC
     2941 GGTATGGCGG GTCGCTGGAT AATCTGCAAA TGCTGCGGCA AGTGTTCGGA CAGCCCGTGG
     3001 TACATCGTCA CGGGTAGCGA GCCTAACAGT GGGCTTATAT TCCGTCATGG GGCCGGGGTT
     3061 CTCCTCCACG TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC TGCCCCCGGG
     3121 GAGCATGTCA AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC AGCATATCAA GGTCAAAGTC
     3181 GTCAAGGGCA TCGGCTGGGA GCATGTCTAA GTCAAAATCG TCAAGGGCGT CGGTCGGCCC
     3241 GCCGCTTTCG CACTTTAGCT GTTTCTCCAG GCCACATATG ATTAGTTCCA GGCCGAAAAG
     3301 GAAGGCAGGT TCGGCTCCCT GCCGGTCGAA CAGCTCAATT GCTTGTTTCA GAAGTGGGGG
     3361 CATAGAATCG GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT ACTTGATGCT CCTGTTCCTC
     3421 CAATACGCAG CCCAGTGTAA AGTGGCCCAC GGCGGACAGA GCGTACAGTG CGTTCTCCAG
     3481 GGAGAAGCCT TGCTGACACA GGAACGCGAG CTGATTTTCC AGGGTTTCGT ACTGTTTCTC
     3541 TGTTGGGCGG GTGCCGAGAT GCACTTTAGC CCCGTCACGA TGTGAGAGGA GAGCACAGCG
     3601 GTATGACTTG GCGTTGTTCC GCAGAAAGTC TTGCCATGAC TCGCCTTCCA GGGGGCAGGA
     3661 GTGGGTATGA TGCCTGTCCA GCATCTCGAT TGGCAGGGCA TCGAGCAGGG CCCGCTTGTT
     3721 CTTCACGTGC CAGTACAGGG TAGGCTGCTC AACTCCCAGC TTTTGAGCGA GTTTCCTTGT
     3781 CGTCAGGCCT TCGATACCGA CTCCATTGAG TAATTCCAGA GCAGAGTTTA TGACTTTGCT
     3841 CTTGTCCAGT CTAGACATCT TATCGTCATC GTCTTTGTAA TCCATGGTGG CGGATCCCGC
     3901 GTCACGACAC CTGTGTTCTG GCGGCAAACC CGTTGCGAAA AAGAACGTTC ACGGCGACTA
     3961 CTGCACTTAT ATACGGTTCT CCCCCACCCT CGGGAAAAAG GCGGAGCCAG TACACGACAT
     4021 CACTTTCCCA GTTTACCCCG CGCCACCTTC TCTAGGCACC GGTTCAATTG CCGACCCCTC
     4081 CCCCCAACTT CTCGGGCACT GTGGGCCATG TGCGCTCTGC CCCATGGTAA TAGCCATGAC
     4141 TAATACGTAG ATGTACTGCC AAGTAGGAAA GTGCCATAAG GTCATGTACT GGGCATAATG
     4201 CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC
     4261 TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA
     4321 AGTGCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC
     4381 GTTGGGCGGT CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA
     4441 TGGGCTATGA ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG
     4501 TCATAAGCTC TAATCGCATA ATCGAGCTAA GAGCGATTAA TATGCTTAGC TAGCTTAGCT
     4561 AAGCTAGATT TCTCTATTCT CCCACAATAT ATAGCAGCTA TTAGCTTAGC AGTCCGAATA
     4621 CGATCGTCCA CGATCCATAA GCGTAACACA TTTGAGTAGC GACTGACGGG CACCGGAGCC
     4681 ACTCGAGTGG AATTATCACC TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGAAGA
     4741 GTTTACTCCC TATCAGTGAT AGAGAACGTA TGCAGACTTT ACTCCCTATC AGTGATAGAG
     4801 AACGTATAAG GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGACCAGT TTACTCCCTA
     4861 TCAGTGATAG AGAACGTATC TACAGTTTAC TCCCTATCAG TGATAGAGAA CGTATATCCA
     4921 GTTTACTCCC TATCAGTGAT AGAGAACGTA TAAGCTTGCC TATATAAGCA GAGCTCGTTT
     4981 AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA
     5041 CCGGGACCGA TCCAGCCTAC CACAAGTTTG TACAAAaaag caggctgcca ccATGGCCAC
     5101 CAATAAGGAG CGACTCTTTG CGGCTGGTGC CCTGGGGCCT GGATCTGGCT ACCCAGGGGC
     5161 AGGTTTCCCC TTCGCCTTCC CAGGGGCACT CAGGGGGTCT CCGCCTTTCG AGATGCTGAG
     5221 CCCTAGCTTC CGGGGCCTGG GCCAGCCTGA CCTCCCCAAG GAGATGGCCT CTCTGTCGGT
     5281 GGAGACACAG AGCACCAGCT CAGAGGAGAT GGTGCCCAGC TCGCCCTCGC CCCCTCCGCC
     5341 TCCTCGGGTC TACAAGCCAT GCTTCGTGTG CAATGACAAG TCCTCTGGCT ACCACTATGG
     5401 GGTCAGCTCT TGTGAAGGCT GCAAGGGCTT CTTTCGCCGA AGCATCCAGA AGAACATGGT
     5461 GTACACGTGT CACCGCGACA AAAACTGTAT CATCAACAAG GTGACCAGGA ATCGCTGCCA
     5521 GTACTGCCGG CTACAGAAGT GCTTCGAAGT GGGCATGTCC AAGGAAGCTG TGCGAAATGA
     5581 CCGGAACAAG AAGAAGAAAG AGGTGAAGGA AGAAGGGTCA CCTGACAGCT ATGAGCTGAG
     5641 CCCTCAGTTA GAAGAGCTCA TCACCAAGGT CAGCAAAGCC CATCAGGAGA CTTTCCCCTC
     5701 GCTCTGCCAG CTGGGCAAGT ATACCACGAA CTCCAGTGCA GACCACCGCG TGCAGCTGGA
     5761 TCTGGGGCTG TGGGACAAGT TCAGTGAGCT GGCTACCAAG TGCATCATCA AGATCGTGGA
     5821 GTTTGCCAAG CGGTTGCCTG GCTTTACAGG GCTCAGCATT GCTGACCAGA TCACTCTGCT
     5881 CAAAGCTGCC TGCCTAGATA TCCTGATGCT GCGTATCTGC ACAAGGTACA CCCCAGAGCA
     5941 GGACACCATG ACCTTCTCCG ACGGGCTGAC CCTGAACCGG ACCCAGATGC ACAATGCCGG
     6001 CTTCGGGCCC CTCACAGACC TTGTCTTTGC CTTTGCTGGG CAGCTCCTGC CCCTGGAGAT
     6061 GGATGACACC GAGACAGGGC TGCTCAGCGC CATCTGCCTC ATCTGCGGAG ACCGCATGGA
     6121 CCTGGAGGAG CCCGAAAAAG TGGACAAGCT GCAGGAGCCA CTGCTGGAAG CCCTGAGGCT
     6181 GTACGCCCGG CGCCGGCGGC CCAGCCAGCC CTACATGTTC CCAAGGATGC TAATGAAAAT
     6241 CACCGACCTC CGGGGCATCA GCACTAAGGG AGCTGAAAGG GCCATTACTC TGAAGATGGA
     6301 GATTCCAGGC CCGATGCCTC CCTTAATCCG AGAGATGCTG GAGAACCCTG AAATGTTTGA
     6361 GGATGACTCC TCGCAGCCTG GTCCCCACCC CAATGCCTCT AGCGAGGATG AGGTTCCTGG
     6421 GGGCCAGGGC AAAGGGGGCC TGAAGTCCCC AGCCTtgcca gctttcttgt acaaaGTGGT
     6481 ATACCCATAC GATGTTCCAG ATTACGCTTA GTAATGAGAA ATACTCAGAA GATGTCACTC
     6541 ACCATAGTCC GCAGTGAAAG ATAAATGATC TAAGGAAGTG AGCCAGATCT GTTTTAGAGC
     6601 TAGAAATAGC AAGTAGGCCA CTGAGCAAGA ACTATGAATG AGATCTTTAG ACATATTGCG
     6661 GCCGCTTTCT CCGCGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCAAAA
     6721 CTAGAATGCA GTGAAAAAAA TGCCTTATTT GTGAAATTTG TGATGCTATT GCCTTATTTG
     6781 TAACCATTAT AAGCTGCAAT AAACAAGTTA TCTATAACAA GAAAATATAT ATATAATAAG
     6841 TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA AATCTTAAAA
     6901 GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG TTCAAAATCA
     6961 GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG ACTGAGATGT
     7021 CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT TTCAAGAATG
     7081 CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAG AATTCACTGG CCGTCGTTTT
     7141 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC
     7201 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT
     7261 GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     7321 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG
     7381 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC
     7441 ATCCGCTTAC AGACAAGCTG TGACCATGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA
     7501 CCGTCATCAC CGAAACACGC GAGTTCGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT
     7561 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG
     7621 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     7681 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT
     7741 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     7801 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAG
//