LOCUS       CK530_TF145_TET1       12860 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            enzyme with enzyme BsrGI 3' end ligation method: enzyme with enzyme
            BsrGI Insert 5' end ligation method: enzyme with enzyme BsrGI 3'
            end ligation method: enzyme with enzyme BsrGI Backbone 5' end
            ligation method: enzyme with enzyme BsrGI 3' end ligation method:
            enzyme with enzyme BsrGI
FEATURES             Location/Qualifiers
     extracted re... 1..379
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..379
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..379
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    380..6787
                     /label="TET1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     attR2           complement(6788..6798)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(6788..6799)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 6788..6862
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             6801..6827
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      6828..6836
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     6840..6862
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     6840..6862
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 6863..6932
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 6867..6889
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6890..6909
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_difference complement(6910..6932)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 6933..6936
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(6937..6959)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(6937..6959)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 6937..6968
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           6960..6984
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 6969..7002
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    6994..7128
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 7003..7158
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 7159..9869
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(7400..7434)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(7446..7461)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(7670..7689)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     7791..7813
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(7852..7870)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        7938..8042
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             8043..8903
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(8261..8280)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      9074..9662
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     9563..9582
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     9816..9833
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 9870..9958
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 9959..12827
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    10039..10060
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        10075..10105
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    10113..10129
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     10137..10153
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   10179..10241
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    10417..10498
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     10525..10544
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(10615..11214)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     11100..11119
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(11215..11271)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(11281..12024)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(11422..11441)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     11667..11686
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(12025..12048)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(12048..12057)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(12077..12288)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(12289..12668)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     12817..12827
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 12828..12860
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(12837..12855)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACt ctcgatcccg ccatgcaagg ccttccagat tagtcaggaa
      421 ggaagatgta aacaaaaaaa agaaaaacag ccaactacga aagacaacca agggagccaa
      481 caaaaatgtg gcatcagtca agactttaag ccctggaaaa ttaaagcaat taattcaaga
      541 aagagatgtt aagaaaaaaa cagaacctaa accacccgtg ccagtcagaa gccttctgac
      601 aagagctgga gcagcacgca tgaatttgga taggactgag gttctttttc agaacccaga
      661 gtccttaacc tgcaatgggt ttacaatggc gctacgaagc acctctctta gcaggcgact
      721 ctcccaaccc ccactggtcg tagccaaatc caaaaaggtt ccactttcta agggtttaga
      781 aaagcaacat gattgtgatt ataagatact ccctgctttg ggagtaaagc actcagaaaa
      841 tgattcggtt ccaatgcaag acacccaagt ccttcctgat atagagactc taattggtgt
      901 acaaaatccc tctttactta aaggtaagag ccaagagaca actcagtttt ggtcccaaag
      961 agttgaggat tccaagatca atatccctac ccacagtggc cctgcagctg agatccttcc
     1021 tgggccactg gaagggacac gctgtggtga aggactattc tctgaagaga cattgaatga
     1081 taccagtggt tccccaaaaa tgtttgctca ggacacagtg tgtgctcctt ttccccaaag
     1141 agcaaccccc aaagttacct ctcaaggaaa ccccagcatt cagttagaag agttgggttc
     1201 acgagtagaa tctcttaagt tatctgattc ttacctggat cccattaaaa gtgaacatga
     1261 ttgctacccc acctccagtc ttaataaggt tatacctgac ttgaacctta gaaactgctt
     1321 ggctcttggt gggtctacgt ctcctacctc tgtaataaaa ttcctcttgg caggctcaaa
     1381 acaagcgacc cttggtgcta aaccagatca tcaagaggcc ttcgaagcta ctgcaaatca
     1441 acaggaagtt tctgatacca cctctttcct aggacaggcc tttggtgcta tcccacatca
     1501 atgggaactt cctggtgctg acccagttca tggtgaggcc ctgggtgaga ccccagatct
     1561 accagagatt cctggtgcta ttccagtcca aggagaggtc tttggtacta ttttagacca
     1621 acaagaaact cttggtatga gtgggagtgt tgtcccagac ttgcctgtct tccttcctgt
     1681 tcctccaaat ccaattgcta cctttaatgc tccttccaaa tggcctgagc cccaaagcac
     1741 tgtctcatat ggacttgcag tccagggtgc tatacagatt ttgcctttgg gctcaggaca
     1801 cactcctcaa tcatcatcaa actcagagaa aaattcatta cctccagtaa tggctataag
     1861 caatgtagaa aatgagaagc aggttcatat aagcttcctg ccagctaaca ctcaggggtt
     1921 cccattagcc cctgagagag gactcttcca tgcttcactg ggtatagccc aactctctca
     1981 ggctggtcct agcaaatcag acagagggag ctcccaggtc agtgtaacca gcacagttca
     2041 tgttgtcaac accacagtgg tgactatgcc agtgccaatg gtcagtacct cctcttcttc
     2101 ctataccact ttgctaccga ctttggaaaa gaagaaaaga aagcgatgtg gggtctgtga
     2161 accctgccag cagaagacca actgtggtga atgcacttac tgcaagaaca gaaagaacag
     2221 ccatcagatc tgtaagaaaa gaaaatgtga ggagctgaaa aagaaaccat ctgttgttgt
     2281 gcctctggag gttataaagg aaaacaagag gccccagagg gaaaagaagc ccaaagtttt
     2341 aaaggcagat tttgacaaca aaccagtaaa tggccccaag tcagaatcca tggactacag
     2401 tagatgtggt catggggaag aacaaaaatt ggaattgaac ccacatactg ttgaaaatgt
     2461 aactaaaaat gaagacagca tgacaggcat cgaggtggag aagtggacac aaaacaagaa
     2521 atcacagtta actgatcacg tgaaaggaga ttttagtgct aatgtcccag aagctgaaaa
     2581 atcgaaaaac tctgaagttg acaagaaacg aaccaaatct ccaaaattgt ttgtacaaac
     2641 cgtaagaaat ggcattaaac atgtacactg tttaccagct gaaacaaatg tttcatttaa
     2701 aaaattcaat attgaagaat tcggcaagac attggaaaac aattcttata aattcctaaa
     2761 agacactgca aaccataaaa acgctatgag ctctgttgct actgatatga gttgtgatca
     2821 tctcaagggg agaagtaacg ttttagtatt ccagcagcct ggctttaact gcagttccat
     2881 tccacattct tcacactcca tcataaatca tcatgctagt atacacaatg aaggtgatca
     2941 accaaaaact cctgagaata taccaagtaa agaaccaaaa gatggatctc ccgttcaacc
     3001 aagtctctta tcgttaatga aagataggag attaacattg gagcaagtgg tagccataga
     3061 ggccctgact caactctcag aagccccatc agagaattcc tccccatcaa agtcagagaa
     3121 ggatgaggaa tcagagcaga gaacagccag tttgcttaat agctgcaaag ctatcctcta
     3181 cactgtaaga aaagacctcc aagacccaaa cttacaggga gagccaccaa aacttaatca
     3241 ctgtccatct ttggaaaaac aaagttcatg caacacggtg gttttcaatg ggcaaactac
     3301 taccctttcc aactcacata tcaactcagc tactaaccaa gcatccacaa agtcacatga
     3361 atattcaaaa gtcacaaatt cattatctct ttttatacca aaatcaaatt catccaagat
     3421 tgacaccaat aaaagtattg ctcaagggat aattactctt gacaattgtt ccaatgattt
     3481 gcatcagttg ccaccaagaa ataatgaagt ggagtattgc aaccagttac tggacagcag
     3541 caaaaaattg gactcagatg atctatcatg tcaggatgca acccataccc aaattgagga
     3601 agatgttgca acacagttga cacaacttgc ttcgataatt aagatcaatt atataaaacc
     3661 agaggacaaa aaagttgaaa gtacaccaac aagccttgtc acatgtaatg tacagcaaaa
     3721 atacaatcag gagaagggca caatgcaaca gaaaccacct tcaagtgtac acaataatca
     3781 tggttcatca ttaacaaaac aaaagaaccc aacccagaaa aagacaaaat ccaccccatc
     3841 aagagatcgg cggaaaaaga agcccacagt tgtaagttat caagaaaatg atcggcagaa
     3901 gtgggaaaag ttgtcctata tgtatggcac aatatgcgac atttggatag catcgaaatt
     3961 tcaaaatttt gggcaatttt gtccacatga ttttcctact gtatttggga aaatttcttc
     4021 ctcgaccaaa atatggaaac cactggctca aacgaggtcc attatgcaac ccaaaacagt
     4081 atttccacca ctcactcaga taaaattaca gagatatcct gaatcagcag aggaaaaggt
     4141 gaaggttgaa ccattggatt cactcagctt atttcatctt aaaacggaat ccaacgggaa
     4201 ggcattcact gataaagctt ataattctca ggtacagtta acggtgaatg ccaatcagaa
     4261 agcccatcct ttgacccagc cctcctctcc acctaaccag tgtgctaacg tgatggcagg
     4321 cgatgaccaa atacggtttc agcaggttgt taaggagcaa ctcatgcatc agagactgcc
     4381 aacattgcct ggtatctctc atgaaacacc cttaccggag tcagcactaa ctctcaggaa
     4441 tgtaaatgta gtgtgttcag gtggaattac agtggtttct accaaaagtg aagaggaagt
     4501 ctgttcatcc agttttggaa catcagaatt ttccacagtg gacagtgcac agaaaaattt
     4561 taatgattat gccatgaact tctttactaa ccctacaaaa aacctagtgt ctataactaa
     4621 agattctgaa ctgcccacct gcagctgtct tgatcgagtt atacaaaaag acaaaggccc
     4681 atattataca caccttgggg caggaccaag tgttgctgct gtcagggaaa tcatggagaa
     4741 taggtatggt caaaaaggaa acgcaataag gatagaaata gtagtgtaca ccggtaaaga
     4801 agggaaaagc tctcatgggt gtccaattgc taagtgggtt ttaagaagaa gcagtgatga
     4861 agaaaaagtt ctttgtttgg tccggcagcg tacaggccac cactgtccaa ctgctgtgat
     4921 ggtggtgctc atcatggtgt gggatggcat ccctcttcca atggccgacc ggctatacac
     4981 agagctcaca gagaatctaa agtcatacaa tgggcaccct accgacagaa gatgcaccct
     5041 caatgaaaat cgtacctgta catgtcaagg aattgatcca gagacttgtg gagcttcatt
     5101 ctcttttggc tgttcatgga gtatgtactt taatggctgt aagtttggta gaagcccaag
     5161 ccccagaaga tttagaattg atccaagctc tcccttacat gaaaaaaacc ttgaagataa
     5221 cttacagagt ttggctacac gattagctcc aatttataag cagtatgctc cagtagctta
     5281 ccaaaatcag gtggaatatg aaaatgttgc ccgagaatgt cggcttggca gcaaggaagg
     5341 tcgtcccttc tctggggtca ctgcttgcct ggacttctgt gctcatcccc acagggacat
     5401 tcacaacatg aataatggaa gcactgtggt ttgtacctta actcgagaag ataaccgctc
     5461 tttgggtgtt attcctcaag atgagcagct ccatgtgcta cctctttata agctttcaga
     5521 cacagatgag tttggctcca aggaaggaat ggaagccaag atcaaatctg gggccatcga
     5581 ggtcctggca ccccgccgca aaaaaagaac gtgtttcact cagcctgttc cccgttctgg
     5641 aaagaagagg gctgcgatga tgacagaggt tcttgcacat aagataaggg cagtggaaaa
     5701 gaaacctatt ccccgaatca agcggaagaa taactcaaca acaacaaaca acagtaagcc
     5761 ttcgtcactg ccaaccttag ggagtaacac tgagaccgtg caacctgaag taaaaagtga
     5821 aaccgaaccc cattttatct taaaaagttc agacaacact aaaacttatt cgctgatgcc
     5881 atccgctcct cacccagtga aagaggcatc tccaggcttc tcctggtccc cgaagactgc
     5941 ttcagccaca ccagctccac tgaagaatga cgcaacagcc tcatgcgggt tttcagaaag
     6001 aagcagcact ccccactgta cgatgccttc gggaagactc agtggtgcca atgcagctgc
     6061 tgctgatggc cctggcattt cacagcttgg cgaagtggct cctctcccca ccctgtctgc
     6121 tcctgtgatg gagcccctca ttaattctga gccttccact ggtgtgactg agccgctaac
     6181 gcctcatcag ccaaaccacc agccctcctt cctcacctct cctcaagacc ttgcctcttc
     6241 tccaatggaa gaagatgagc agcattctga agcagatgag cctccatcag acgaacccct
     6301 atctgatgac cccctgtcac ctgctgagga gaaattgccc cacattgatg agtattggtc
     6361 agacagtgag cacatctttt tggatgcaaa tattggtggg gtggccatcg cacctgctca
     6421 cggctcggtt ttgattgagt gtgcccggcg agagctgcac gctaccactc ctgttgagca
     6481 ccccaaccgt aatcatccaa cccgcctctc ccttgtcttt taccagcaca aaaacctaaa
     6541 taagccccaa catggttttg aactaaacaa gattaagttt gaggctaaag aagctaagaa
     6601 taagaaaatg aaggcctcag agcaaaaaga ccaggcagct aatgaaggtc cagaacagtc
     6661 ctctgaagta aatgaattga accaaattcc ttctcataaa gcattaacat taacccatga
     6721 caatgttgtc accgtgtccc cttatgctct cacacacgtt gcggggccct ataaccattg
     6781 ggtctgaGTA CAAAGTGGTA TACCCATACG ATGTTCCAGA TTACGCTTAG TAATGAGAAA
     6841 TACTCAGAAG ATGTCACTCA CCATAGTCCG CAGTGAAAGA TAAATGATCG GTATTTTGCC
     6901 CGTGATTCTG TTTTAGAGCT AGAAATAGCA AGTAGGCCAC TGAGCAAGAA CTATGAATGA
     6961 GATCTTTAGA CATATTGCGG CCGCTTTCTC CGCGATCCAG ACATGATAAG ATACATTGAT
     7021 GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT GCCTTATTTG TGAAATTTGT
     7081 GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAT CTATAACAAG
     7141 AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG AAATAACAAT ATAATTATCG
     7201 TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG CGTCATTTTG ACTCACGCGG
     7261 TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA AGCACGCCTC ACGGGAGCTC
     7321 CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT TCGCGCTATT TAGAAAGAGA
     7381 GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA CTATCTTTCT AGGGTTAAGA
     7441 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
     7501 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
     7561 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC
     7621 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT
     7681 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
     7741 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCATGTCT CCGGGAGCTG
     7801 CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACACGCG AGTTCGAAAG GGCCTCGTGA
     7861 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
     7921 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
     7981 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
     8041 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
     8101 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
     8161 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
     8221 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
     8281 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
     8341 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
     8401 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
     8461 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC
     8521 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
     8581 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
     8641 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
     8701 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGTT
     8761 CACGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT
     8821 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
     8881 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
     8941 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
     9001 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     9061 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     9121 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     9181 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     9241 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     9301 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     9361 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     9421 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
     9481 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     9541 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     9601 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     9661 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     9721 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
     9781 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
     9841 ATGAGCGCCC AATACGCAAA CCGCCTCTCA CCGCGCGTTG GCCGATTCAT TAATGCAGCT
     9901 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAAAC CGCCTCTCCC
     9961 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG
    10021 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA
    10081 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG
    10141 AAACAGCTAT GACCATGATT ACGCCAAGGT CGACTTAACC CTAGAAAGAT AATCATATTG
    10201 TGACGTACGT TAAAGATAAT CATGCGTAAA ATTGACGCAT GTGTTTTATC GGTCTGTATA
    10261 TCGAGGTTTA TTTATTAATT TGAATAGATA TTAAGTTTTA TTATATTTAC ACTTACATAC
    10321 TAATAATAAA TTCAACAAAC AATTTATTTA TGTTTATTTA TTTATTAAAA AAAAACAAAA
    10381 ACTCAAAATT TCTTCTATAA AGTAACAAAA CTTTTAGCAG TGAAAAAAAT GCTTTATTTG
    10441 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA
    10501 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA
    10561 AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTGG AGATTCATGC
    10621 GCCAGGTTTT CTGGTCATGC ACCACGTTCG TGGGCCCTCA GGAACCTCCA CGTCAGCGGT
    10681 AACTGTGAAG CCCAGTCGCT CGTAAAAAGG AAGATTCCGG GGAGCACTGG TTTCGAGGAA
    10741 CGCCGGCACG CCAGCCCGTT CCGCGGCCTC AACACCAGGA AGAACGACAG CGCTTCCCAA
    10801 ACCTTTACCT TGGTGATCTG GGCTGACACC GACAGTGGCA AGAAACCACG CAGGCTCCTT
    10861 TGGTCTATGA GGAGCCAGCA AGCCCTCCAT TTGCTGTTGT GCCGCCAACC TACTCCCTGA
    10921 CAGCTCGGCC ATCCGCGGAC CTATTTCCGC GAACACTGCG CCGGCTTCGA CGCTTTCCGG
    10981 AGTAGTCCAG ACCGCAACTG CGGCACCATC GTCCGCAACC CAAACTTTAC CAATGTCCAG
    11041 ACCCACGCGC GTAAGAAACA GCTCTTGCAA CTCAGTTACC CTCTCTATGT GTCTGTCCGG
    11101 ATCTACGGTA TGGCGGGTCG CTGGATAATC TGCAAATGCT GCGGCAAGTG TTCGGACAGC
    11161 CCGTGGTACA TCGTCACGGG TAGCGAGCCT AACAGTGGGC TTATATTCCG TCATGGGGCC
    11221 GGGGTTCTCC TCCACGTCGC CGGCCTGCTT CAGCAGGCTG AAGTTGGTGG CGCCGCTGCC
    11281 CCCGGGGAGC ATGTCAAGGT CAAAATCGTC AAGAGCGTCA GCAGGCAGCA TATCAAGGTC
    11341 AAAGTCGTCA AGGGCATCGG CTGGGAGCAT GTCTAAGTCA AAATCGTCAA GGGCGTCGGT
    11401 CGGCCCGCCG CTTTCGCACT TTAGCTGTTT CTCCAGGCCA CATATGATTA GTTCCAGGCC
    11461 GAAAAGGAAG GCAGGTTCGG CTCCCTGCCG GTCGAACAGC TCAATTGCTT GTTTCAGAAG
    11521 TGGGGGCATA GAATCGGTGG TAGGTGTCTC TCTTTCCTCT TTTGCTACTT GATGCTCCTG
    11581 TTCCTCCAAT ACGCAGCCCA GTGTAAAGTG GCCCACGGCG GACAGAGCGT ACAGTGCGTT
    11641 CTCCAGGGAG AAGCCTTGCT GACACAGGAA CGCGAGCTGA TTTTCCAGGG TTTCGTACTG
    11701 TTTCTCTGTT GGGCGGGTGC CGAGATGCAC TTTAGCCCCG TCACGATGTG AGAGGAGAGC
    11761 ACAGCGGTAT GACTTGGCGT TGTTCCGCAG AAAGTCTTGC CATGACTCGC CTTCCAGGGG
    11821 GCAGGAGTGG GTATGATGCC TGTCCAGCAT CTCGATTGGC AGGGCATCGA GCAGGGCCCG
    11881 CTTGTTCTTC ACGTGCCAGT ACAGGGTAGG CTGCTCAACT CCCAGCTTTT GAGCGAGTTT
    11941 CCTTGTCGTC AGGCCTTCGA TACCGACTCC ATTGAGTAAT TCCAGAGCAG AGTTTATGAC
    12001 TTTGCTCTTG TCCAGTCTAG ACATCTTATC GTCATCGTCT TTGTAATCCA TGGTGGCGGA
    12061 TCCCGCGTCA CGACACCTGT GTTCTGGCGG CAAACCCGTT GCGAAAAAGA ACGTTCACGG
    12121 CGACTACTGC ACTTATATAC GGTTCTCCCC CACCCTCGGG AAAAAGGCGG AGCCAGTACA
    12181 CGACATCACT TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA GGCACCGGTT CAATTGCCGA
    12241 CCCCTCCCCC CAACTTCTCG GGCACTGTGG GCCATGTGCG CTCTGCCCCA TGGTAATAGC
    12301 CATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTGC CATAAGGTCA TGTACTGGGC
    12361 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG
    12421 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA
    12481 ATGGAAAGTG CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG
    12541 GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC
    12601 CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA
    12661 TCAATGTCAT AAGCTCTAAT CGCATAATCG AGCTAAGAGC GATTAATATG CTTAGCTAGC
    12721 TTAGCTAAGC TAGATTTCTC TATTCTCCCA CAATATATAG CAGCTATTAG CTTAGCAGTC
    12781 CGAATACGAT CGTCCACGAT CCATAAGCGT AACACATTTG AGTAGCGACT GACGGGCACC
    12841 GGAGCCACTC GAGTGGAATT
//