LOCUS       CK530_TF149_PAX5        7660 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            enzyme with enzyme BsrGI 3' end ligation method: enzyme with enzyme
            BsrGI Insert 5' end ligation method: enzyme with enzyme BsrGI 3'
            end ligation method: enzyme with enzyme BsrGI Backbone 5' end
            ligation method: enzyme with enzyme BsrGI 3' end ligation method:
            enzyme with enzyme BsrGI
FEATURES             Location/Qualifiers
     extracted re... 1..379
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..379
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..379
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1588..1598)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1588..1599)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1588..1662
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1601..1627
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1628..1636
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1640..1662
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     1640..1662
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1663..1732
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1667..1689
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1690..1709
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_difference complement(1710..1732)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1733..1736
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1737..1759)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1737..1759)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 1737..1768
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1760..1784
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1769..1802
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    1794..1928
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1803..1958
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1959..4669
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2200..2234)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2246..2261)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(2470..2489)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2591..2613
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2652..2670)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2738..2842
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2843..3703
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3061..3080)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      3874..4462
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4363..4382
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4616..4633
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4670..4758
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4759..7627
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4839..4860
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4875..4905
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4913..4929
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4937..4953
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4979..5041
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5217..5298
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5325..5344
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5415..6014)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5900..5919
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6015..6071)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6081..6824)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6222..6241)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6467..6486
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(6825..6848)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6848..6857)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6877..7088)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7089..7468)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7617..7627
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7628..7660
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7637..7655)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTGCACCATG GATTTAGAGA AAAATTATCC
      421 GACTCCTCGG ACCAGCAGGA CAGGACATGG AGGAGTGAAT CAGCTTGGGG GGGTTTTTGT
      481 GAATGGACGG CCACTCCCGG ATGTAGTCCG CCAGAGGATA GTGGAACTTG CTCATCAAGG
      541 TGTCAGGCCC TGCGACATCT CCAGGCAGCT TCGGGTCAGC CATGGTTGTG TCAGCAAAAT
      601 TCTTGGCAGG TATTATGAGA CAGGAAGCAT CAAGCCTGGG GTAATTGGAG GATCCAAACC
      661 AAAGGTCGCC ACACCCAAAG TGGTGGAAAA AATCGCTGAA TATAAACGCC AAAATCCCAC
      721 CATGTTTGCC TGGGAGATCA GGGACCGGCT GCTGGCAGAG CGGGTGTGTG ACAATGACAC
      781 CGTGCCTAGC GTCAGTTCCA TCAACAGGAT CATCCGGACA AAAGTACAGC AGCCACCCAA
      841 CCAACCAGTC CCAGCTTCCA GTCACAGCAT AGTGTCCACT GGCTCCGTGA CGCAGGTGTC
      901 CTCGGTGAGC ACGGATTCGG CCGGCTCGTC GTACTCCATC AGCGGCATCC TGGGCATCAC
      961 GTCCCCCAGC GCCGACACCA ACAAGCGCAA GAGAGACGAA GGTATTCAGG AGTCTCCGGT
     1021 GCCGAACGGC CACTCGCTTC CGGGCAGAGA CTTCCTCCGG AAGCAGATGC GGGGAGACTT
     1081 GTTCACACAG CAGCAGCTGG AGGTGCTGGA CCGCGTGTTT GAGAGGCAGC ACTACTCAGA
     1141 CATCTTCACC ACCACAGAGC CCATCAAGCC CGAGCAGACC ACAGAGTATT CAGCCATGGC
     1201 CTCGCTGGCT GGTGGGCTGG ACGACATGAA GGCCAATCTG GCCAGCCCCA CCCCTGCTGA
     1261 CATCGGGAGC AGTGTGCCAG GCCCGCAGTC CTACCCCATT GTGACAGGCC GTGACTTGGC
     1321 GAGCACGACC CTCCCCGGGT ACCCTCCACA CGTCCCCCCC GCTGGACAGG GCAGCTACTC
     1381 AGCACCGACG CTGACAGGGA TGGTGCCTGG GAGTGAGTTT TCCGGGAGTC CCTACAGCCA
     1441 CCCTCAGTAT TCCTCGTACA ACGACTCCTG GAGGTTCCCC AACCCGGGGC TGCTTGGCTC
     1501 CCCCTACTAT TATAGCGCTG CCGCCCGAGG AGCCGCCCCA CCTGCAGCCG CCACTGCCTA
     1561 TGACCGTCAC TGAGACCCAG CTTTCTTGTA CAAAGTGGTA TACCCATACG ATGTTCCAGA
     1621 TTACGCTTAG TAATGAGAAA TACTCAGAAG ATGTCACTCA CCATAGTCCG CAGTGAAAGA
     1681 TAAATGATCG CTGCCTCTCA GATTTCTGAG TTTTAGAGCT AGAAATAGCA AGTAGGCCAC
     1741 TGAGCAAGAA CTATGAATGA GATCTTTAGA CATATTGCGG CCGCTTTCTC CGCGATCCAG
     1801 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT
     1861 GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA
     1921 AACAAGTTAT CTATAACAAG AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG
     1981 AAATAACAAT ATAATTATCG TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG
     2041 CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA
     2101 AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT
     2161 TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA
     2221 CTATCTTTCT AGGGTTAAGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     2281 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     2341 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     2401 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
     2461 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
     2521 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
     2581 GACCATGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACACGCG
     2641 AGTTCGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
     2701 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
     2761 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
     2821 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
     2881 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
     2941 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
     3001 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
     3061 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
     3121 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
     3181 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
     3241 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
     3301 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
     3361 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
     3421 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
     3481 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
     3541 TGGAGCCGGT GAGCGTGGTT CACGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
     3601 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
     3661 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
     3721 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
     3781 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
     3841 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     3901 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     3961 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     4021 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     4081 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     4141 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     4201 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     4261 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     4321 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     4381 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     4441 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
     4501 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
     4561 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
     4621 GTCAGTGAGC GAGGAAGCGG ATGAGCGCCC AATACGCAAA CCGCCTCTCA CCGCGCGTTG
     4681 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     4741 CAACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
     4801 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
     4861 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
     4921 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGGT CGACTTAACC
     4981 CTAGAAAGAT AATCATATTG TGACGTACGT TAAAGATAAT CATGCGTAAA ATTGACGCAT
     5041 GTGTTTTATC GGTCTGTATA TCGAGGTTTA TTTATTAATT TGAATAGATA TTAAGTTTTA
     5101 TTATATTTAC ACTTACATAC TAATAATAAA TTCAACAAAC AATTTATTTA TGTTTATTTA
     5161 TTTATTAAAA AAAAACAAAA ACTCAAAATT TCTTCTATAA AGTAACAAAA CTTTTAGCAG
     5221 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
     5281 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
     5341 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
     5401 GATCCTCTGG AGATTCATGC GCCAGGTTTT CTGGTCATGC ACCACGTTCG TGGGCCCTCA
     5461 GGAACCTCCA CGTCAGCGGT AACTGTGAAG CCCAGTCGCT CGTAAAAAGG AAGATTCCGG
     5521 GGAGCACTGG TTTCGAGGAA CGCCGGCACG CCAGCCCGTT CCGCGGCCTC AACACCAGGA
     5581 AGAACGACAG CGCTTCCCAA ACCTTTACCT TGGTGATCTG GGCTGACACC GACAGTGGCA
     5641 AGAAACCACG CAGGCTCCTT TGGTCTATGA GGAGCCAGCA AGCCCTCCAT TTGCTGTTGT
     5701 GCCGCCAACC TACTCCCTGA CAGCTCGGCC ATCCGCGGAC CTATTTCCGC GAACACTGCG
     5761 CCGGCTTCGA CGCTTTCCGG AGTAGTCCAG ACCGCAACTG CGGCACCATC GTCCGCAACC
     5821 CAAACTTTAC CAATGTCCAG ACCCACGCGC GTAAGAAACA GCTCTTGCAA CTCAGTTACC
     5881 CTCTCTATGT GTCTGTCCGG ATCTACGGTA TGGCGGGTCG CTGGATAATC TGCAAATGCT
     5941 GCGGCAAGTG TTCGGACAGC CCGTGGTACA TCGTCACGGG TAGCGAGCCT AACAGTGGGC
     6001 TTATATTCCG TCATGGGGCC GGGGTTCTCC TCCACGTCGC CGGCCTGCTT CAGCAGGCTG
     6061 AAGTTGGTGG CGCCGCTGCC CCCGGGGAGC ATGTCAAGGT CAAAATCGTC AAGAGCGTCA
     6121 GCAGGCAGCA TATCAAGGTC AAAGTCGTCA AGGGCATCGG CTGGGAGCAT GTCTAAGTCA
     6181 AAATCGTCAA GGGCGTCGGT CGGCCCGCCG CTTTCGCACT TTAGCTGTTT CTCCAGGCCA
     6241 CATATGATTA GTTCCAGGCC GAAAAGGAAG GCAGGTTCGG CTCCCTGCCG GTCGAACAGC
     6301 TCAATTGCTT GTTTCAGAAG TGGGGGCATA GAATCGGTGG TAGGTGTCTC TCTTTCCTCT
     6361 TTTGCTACTT GATGCTCCTG TTCCTCCAAT ACGCAGCCCA GTGTAAAGTG GCCCACGGCG
     6421 GACAGAGCGT ACAGTGCGTT CTCCAGGGAG AAGCCTTGCT GACACAGGAA CGCGAGCTGA
     6481 TTTTCCAGGG TTTCGTACTG TTTCTCTGTT GGGCGGGTGC CGAGATGCAC TTTAGCCCCG
     6541 TCACGATGTG AGAGGAGAGC ACAGCGGTAT GACTTGGCGT TGTTCCGCAG AAAGTCTTGC
     6601 CATGACTCGC CTTCCAGGGG GCAGGAGTGG GTATGATGCC TGTCCAGCAT CTCGATTGGC
     6661 AGGGCATCGA GCAGGGCCCG CTTGTTCTTC ACGTGCCAGT ACAGGGTAGG CTGCTCAACT
     6721 CCCAGCTTTT GAGCGAGTTT CCTTGTCGTC AGGCCTTCGA TACCGACTCC ATTGAGTAAT
     6781 TCCAGAGCAG AGTTTATGAC TTTGCTCTTG TCCAGTCTAG ACATCTTATC GTCATCGTCT
     6841 TTGTAATCCA TGGTGGCGGA TCCCGCGTCA CGACACCTGT GTTCTGGCGG CAAACCCGTT
     6901 GCGAAAAAGA ACGTTCACGG CGACTACTGC ACTTATATAC GGTTCTCCCC CACCCTCGGG
     6961 AAAAAGGCGG AGCCAGTACA CGACATCACT TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA
     7021 GGCACCGGTT CAATTGCCGA CCCCTCCCCC CAACTTCTCG GGCACTGTGG GCCATGTGCG
     7081 CTCTGCCCCA TGGTAATAGC CATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTGC
     7141 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
     7201 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
     7261 TACTCCACCC ATTGACGTCA ATGGAAAGTG CCTATTGGCG TTACTATGGG AACATACGTC
     7321 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
     7381 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC
     7441 TATTAATAAC TAGTCAATAA TCAATGTCAT AAGCTCTAAT CGCATAATCG AGCTAAGAGC
     7501 GATTAATATG CTTAGCTAGC TTAGCTAAGC TAGATTTCTC TATTCTCCCA CAATATATAG
     7561 CAGCTATTAG CTTAGCAGTC CGAATACGAT CGTCCACGAT CCATAAGCGT AACACATTTG
     7621 AGTAGCGACT GACGGGCACC GGAGCCACTC GAGTGGAATT
//