LOCUS       CK530_TF150_PAX6a       7753 bp ds-DNA     circular     10-JUN-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            enzyme with enzyme BsrGI 3' end ligation method: enzyme with enzyme
            BsrGI Insert 5' end ligation method: enzyme with enzyme BsrGI 3'
            end ligation method: enzyme with enzyme BsrGI Backbone 5' end
            ligation method: enzyme with enzyme BsrGI 3' end ligation method:
            enzyme with enzyme BsrGI
FEATURES             Location/Qualifiers
     extracted re... 1..379
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    368..379
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..379
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1681..1691)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1681..1692)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1681..1755
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1694..1720
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1721..1729
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1733..1755
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     1733..1755
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     extracted re... 1756..1825
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_difference 1760..1782
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1783..1802
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_difference complement(1803..1825)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 1826..1829
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(1830..1852)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(1830..1852)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     extracted re... 1830..1861
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     motif           1853..1877
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1862..1895
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    1887..2021
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1896..2051
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 2052..4762
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2293..2327)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2339..2354)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(2563..2582)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2684..2706
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(2745..2763)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2831..2935
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2936..3796
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(3154..3173)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      3967..4555
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4456..4475
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4709..4726
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4763..4851
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4852..7720
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4932..4953
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4968..4998
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    5006..5022
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     5030..5046
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   5072..5134
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5310..5391
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5418..5437
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5508..6107)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     5993..6012
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6108..6164)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6174..6917)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6315..6334)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     6560..6579
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(6918..6941)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6941..6950)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6970..7181)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7182..7561)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7710..7720
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7721..7753
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7730..7748)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTGCACCATG CAGAACAGTC ACAGCGGAGT
      421 GAATCAGCTC GGTGGTGTCT TTGTCAACGG GCGGCCACTG CCGGACTCCA CCCGGCAGAA
      481 GATTGTAGAG CTAGCTCACA GCGGGGCCCG GCCGTGCGAC ATTTCCCGAA TTCTGCAGGT
      541 GTCCAACGGA TGTGTGAGTA AAATTCTGGG CAGGTATTAC GAGACTGGCT CCATCAGACC
      601 CAGGGCAATC GGTGGTAGTA AACCGAGAGT AGCGACTCCA GAAGTTGTAA GCAAAATAGC
      661 CCAGTATAAG CGGGAGTGCC CGTCCATCTT TGCTTGGGAA ATCCGAGACA GATTACTGTC
      721 CGAGGGGGTC TGTACCAACG ATAACATACC AAGCGTGTCA TCAATAAACA GAGTTCTTCG
      781 CAACCTGGCT AGCGAAAAGC AACAGATGGG CGCAGACGGC ATGTATGATA AACTAAGGAT
      841 GTTGAACGGG CAGACCGGAA GCTGGGGCAC CCGCCCTGGT TGGTATCCGG GGACTTCGGT
      901 GCCAGGGCAA CCTACGCAAG ATGGCTGCCA GCAACAGGAA GGAGGGGGAG AGAATACCAA
      961 CTCCATCAGT TCCAACGGAG AAGATTCAGA TGAGGCTCAA ATGCGACTTC AGCTGAAGCG
     1021 GAAGCTGCAA AGAAATAGAA CATCCTTTAC CCAAGAGCAA ATTGAGGCCC TGGAGAAAGA
     1081 GTTTGAGAGA ACCCATTATC CAGATGTGTT TGCCCGAGAA AGACTAGCAG CCAAAATAGA
     1141 TCTACCTGAA GCAAGAATAC AGGTATGGTT TTCTAATCGA AGGGCCAAAT GGAGAAGAGA
     1201 AGAAAAACTG AGGAATCAGA GAAGACAGGC CAGCAACACA CCTAGTCATA TTCCTATCAG
     1261 CAGTAGTTTC AGCACCAGTG TCTACCAACC AATTCCACAA CCCACCACAC CGGTTTCCTC
     1321 CTTCACATCT GGCTCCATGT TGGGCCGAAC AGACACAGCC CTCACAAACA CCTACAGCGC
     1381 TCTGCCGCCT ATGCCCAGCT TCACCATGGC AAATAACCTG CCTATGCAAC CCCCAGTCCC
     1441 CAGCCAGACC TCCTCATACT CCTGCATGCT GCCCACCAGC CCTTCGGTGA ATGGGCGGAG
     1501 TTATGATACC TACACCCCCC CACATATGCA GACACACATG AACAGTCAGC CAATGGGCAC
     1561 CTCGGGCACC ACTTCAACAG GACTCATTTC CCCTGGTGTG TCAGTTCCAG TTCAAGTTCC
     1621 CGGAAGTGAA CCTGATATGT CTCAATACTG GCCAAGATTA CAGTAAGACC CAGCTTTCTT
     1681 GTACAAAGTG GTATACCCAT ACGATGTTCC AGATTACGCT TAGTAATGAG AAATACTCAG
     1741 AAGATGTCAC TCACCATAGT CCGCAGTGAA AGATAAATGA TCCTTTTCAA ATAAGACGTC
     1801 TAGTTTTAGA GCTAGAAATA GCAAGTAGGC CACTGAGCAA GAACTATGAA TGAGATCTTT
     1861 AGACATATTG CGGCCGCTTT CTCCGCGATC CAGACATGAT AAGATACATT GATGAGTTTG
     1921 GACAAACCAA AACTAGAATG CAGTGAAAAA AATGCCTTAT TTGTGAAATT TGTGATGCTA
     1981 TTGCCTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TATCTATAAC AAGAAAATAT
     2041 ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA TCGTATGAGT
     2101 TAAATCTTAA AAGTCACGTA AAAGATAATC ATGCGTCATT TTGACTCACG CGGTCGTTAT
     2161 AGTTCAAAAT CAGTGACACT TACCGCATTG ACAAGCACGC CTCACGGGAG CTCCAAGCGG
     2221 CGACTGAGAT GTCCTAAATG CACAGCGACG GATTCGCGCT ATTTAGAAAG AGAGAGCAAT
     2281 ATTTCAAGAA TGCATGCGTC AATTTTACGC AGACTATCTT TCTAGGGTTA AGAATTCACT
     2341 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT
     2401 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC
     2461 TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCCTG ATGCGGTATT TTCTCCTTAC
     2521 GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC
     2581 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     2641 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCATG TCTCCGGGAG CTGCATGTGT
     2701 CAGAGGTTTT CACCGTCATC ACCGAAACAC GCGAGTTCGA AAGGGCCTCG TGATACGCCT
     2761 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG
     2821 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
     2881 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
     2941 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
     3001 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
     3061 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
     3121 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT
     3181 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
     3241 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG
     3301 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
     3361 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG
     3421 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT
     3481 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
     3541 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC
     3601 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GTTCACGCGG
     3661 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC
     3721 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
     3781 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA
     3841 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
     3901 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
     3961 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
     4021 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
     4081 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
     4141 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
     4201 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
     4261 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
     4321 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
     4381 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
     4441 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
     4501 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
     4561 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
     4621 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC
     4681 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGATGAGCG
     4741 CCCAATACGC AAACCGCCTC TCACCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA
     4801 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA AACCGCCTCT CCCCGCGCGT
     4861 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
     4921 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
     4981 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
     5041 TATGACCATG ATTACGCCAA GGTCGACTTA ACCCTAGAAA GATAATCATA TTGTGACGTA
     5101 CGTTAAAGAT AATCATGCGT AAAATTGACG CATGTGTTTT ATCGGTCTGT ATATCGAGGT
     5161 TTATTTATTA ATTTGAATAG ATATTAAGTT TTATTATATT TACACTTACA TACTAATAAT
     5221 AAATTCAACA AACAATTTAT TTATGTTTAT TTATTTATTA AAAAAAAACA AAAACTCAAA
     5281 ATTTCTTCTA TAAAGTAACA AAACTTTTAG CAGTGAAAAA AATGCTTTAT TTGTGAAATT
     5341 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC
     5401 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
     5461 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCCTC TGGAGATTCA TGCGCCAGGT
     5521 TTTCTGGTCA TGCACCACGT TCGTGGGCCC TCAGGAACCT CCACGTCAGC GGTAACTGTG
     5581 AAGCCCAGTC GCTCGTAAAA AGGAAGATTC CGGGGAGCAC TGGTTTCGAG GAACGCCGGC
     5641 ACGCCAGCCC GTTCCGCGGC CTCAACACCA GGAAGAACGA CAGCGCTTCC CAAACCTTTA
     5701 CCTTGGTGAT CTGGGCTGAC ACCGACAGTG GCAAGAAACC ACGCAGGCTC CTTTGGTCTA
     5761 TGAGGAGCCA GCAAGCCCTC CATTTGCTGT TGTGCCGCCA ACCTACTCCC TGACAGCTCG
     5821 GCCATCCGCG GACCTATTTC CGCGAACACT GCGCCGGCTT CGACGCTTTC CGGAGTAGTC
     5881 CAGACCGCAA CTGCGGCACC ATCGTCCGCA ACCCAAACTT TACCAATGTC CAGACCCACG
     5941 CGCGTAAGAA ACAGCTCTTG CAACTCAGTT ACCCTCTCTA TGTGTCTGTC CGGATCTACG
     6001 GTATGGCGGG TCGCTGGATA ATCTGCAAAT GCTGCGGCAA GTGTTCGGAC AGCCCGTGGT
     6061 ACATCGTCAC GGGTAGCGAG CCTAACAGTG GGCTTATATT CCGTCATGGG GCCGGGGTTC
     6121 TCCTCCACGT CGCCGGCCTG CTTCAGCAGG CTGAAGTTGG TGGCGCCGCT GCCCCCGGGG
     6181 AGCATGTCAA GGTCAAAATC GTCAAGAGCG TCAGCAGGCA GCATATCAAG GTCAAAGTCG
     6241 TCAAGGGCAT CGGCTGGGAG CATGTCTAAG TCAAAATCGT CAAGGGCGTC GGTCGGCCCG
     6301 CCGCTTTCGC ACTTTAGCTG TTTCTCCAGG CCACATATGA TTAGTTCCAG GCCGAAAAGG
     6361 AAGGCAGGTT CGGCTCCCTG CCGGTCGAAC AGCTCAATTG CTTGTTTCAG AAGTGGGGGC
     6421 ATAGAATCGG TGGTAGGTGT CTCTCTTTCC TCTTTTGCTA CTTGATGCTC CTGTTCCTCC
     6481 AATACGCAGC CCAGTGTAAA GTGGCCCACG GCGGACAGAG CGTACAGTGC GTTCTCCAGG
     6541 GAGAAGCCTT GCTGACACAG GAACGCGAGC TGATTTTCCA GGGTTTCGTA CTGTTTCTCT
     6601 GTTGGGCGGG TGCCGAGATG CACTTTAGCC CCGTCACGAT GTGAGAGGAG AGCACAGCGG
     6661 TATGACTTGG CGTTGTTCCG CAGAAAGTCT TGCCATGACT CGCCTTCCAG GGGGCAGGAG
     6721 TGGGTATGAT GCCTGTCCAG CATCTCGATT GGCAGGGCAT CGAGCAGGGC CCGCTTGTTC
     6781 TTCACGTGCC AGTACAGGGT AGGCTGCTCA ACTCCCAGCT TTTGAGCGAG TTTCCTTGTC
     6841 GTCAGGCCTT CGATACCGAC TCCATTGAGT AATTCCAGAG CAGAGTTTAT GACTTTGCTC
     6901 TTGTCCAGTC TAGACATCTT ATCGTCATCG TCTTTGTAAT CCATGGTGGC GGATCCCGCG
     6961 TCACGACACC TGTGTTCTGG CGGCAAACCC GTTGCGAAAA AGAACGTTCA CGGCGACTAC
     7021 TGCACTTATA TACGGTTCTC CCCCACCCTC GGGAAAAAGG CGGAGCCAGT ACACGACATC
     7081 ACTTTCCCAG TTTACCCCGC GCCACCTTCT CTAGGCACCG GTTCAATTGC CGACCCCTCC
     7141 CCCCAACTTC TCGGGCACTG TGGGCCATGT GCGCTCTGCC CCATGGTAAT AGCCATGACT
     7201 AATACGTAGA TGTACTGCCA AGTAGGAAAG TGCCATAAGG TCATGTACTG GGCATAATGC
     7261 CAGGCGGGCC ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT ATGATACACT
     7321 TGATGTACTG CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG TCAATGGAAA
     7381 GTGCCTATTG GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG GCGGGGGTCG
     7441 TTGGGCGGTC AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCGGAA CTCCATATAT
     7501 GGGCTATGAA CTAATGACCC CGTAATTGAT TACTATTAAT AACTAGTCAA TAATCAATGT
     7561 CATAAGCTCT AATCGCATAA TCGAGCTAAG AGCGATTAAT ATGCTTAGCT AGCTTAGCTA
     7621 AGCTAGATTT CTCTATTCTC CCACAATATA TAGCAGCTAT TAGCTTAGCA GTCCGAATAC
     7681 GATCGTCCAC GATCCATAAG CGTAACACAT TTGAGTAGCG ACTGACGGGC ACCGGAGCCA
     7741 CTCGAGTGGA ATT
//