LOCUS       CK530-H_TF110_HOXB5     7597 bp ds-DNA     circular     13-MAR-2024
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(322..344)
                     /label="MPS467_EF1a-mTagBFP2-Gibson"
                     /note="sequence: TTCCTTTATGAGTTCACTCATGGTGGCtcttctatggaggtcaaaacagc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..1204
                     /label="HOXB5"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             398..1258
                     /label="Translation 398-1258"
                     /translation="MSSYFVNSFSGRYPNGPDYQLLNYGSGSSLSGSYRDPAAMHTGSYGYNYNGMDLSVNRSSASSSHFGAVGESSRAFPAPAQEPRFRQAASSCSLSSPESLPCTNGDSHGAKPSASSPSDQATSASSSANFTEIDEASASSEPEEAASQLSSPSLARAQPEPMATSTAAPEGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQPLPTFLYKVVYPYDVPDYA"
     primer          955..973
                     /label="MPS556"
                     /note="sequence: ccatgatatgaccgggccg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1208..1227
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1209..1227)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1213..1229)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1213..1230)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1213..1258
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1218..1227
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1227..1281
                     /label="MPS505_barcode_deletion_Gibson"
                     /note="sequence: aaGTGGTATACCCATACGATGTTCCAGATTACGCTCTTTCTCCGCGATCCAGACA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1232..1258
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1232..1258)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1234..1266)
                     /label="MPS506_HA_stop_Gibson"
                     /note="sequence: GAAAGttaAGCGTAATCTGGAACATCGTATGGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1237..1281
                     /label="MPS507_barcode_deletion_Gibson_with_stop"
                     /note="sequence: ATACGATGTTCCAGATTACGCTtaaCTTTCTCCGCGATCCAGACA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     motif           1262..1262
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1262..1280
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1262..1281
                     /label="MPS505_barcode_deletion_Gibson"
                     /note="sequence: aaGTGGTATACCCATACGATGTTCCAGATTACGCTCTTTCTCCGCGATCCAGACA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1262..1297
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="LSPRSRHDKIH*"
     primer          complement(1268..1289)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    1272..1406
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1281..1436
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1437..4147
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(1678..1712)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(1724..1739)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1724..1739)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1743..1759)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(1948..1967)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2069..2091
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2069..2091
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2130..2148)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2204..2223
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        2216..2320
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2321..3181
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2539..2558)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2539..2558)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3352..3940
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     3841..3860
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4094..4111
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4148..4236
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4237..7531
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4317..4338
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4353..4383
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4391..4407
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4398..4417
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     4415..4431
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4457..4519
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4695..4776
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     4803..4822
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4810..4826
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4857..4876
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          4863..4912
                     /label="MPS317_HygR_rev"
                     /note="sequence: TGGTATGGCTGATTATGATCCTCTGGAGATctattcctttgccctcggac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4869..4892)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4893..4912
                     /label="MPS317_HygR_rev"
                     /note="sequence: TGGTATGGCTGATTATGATCCTCTGGAGATctattcctttgccctcggac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(4893..5918)
                     /label="HygR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(4893..5918)
                     /label="Translation 5918-4893"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
     primer          complement(5898..5942)
                     /label="MPS316_P2A-HygR"
                     /note="sequence: GACGTGGAGGAGAACCCCGGCCCCatgaaaaagcctgaactcacc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          5919..5936
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(5919..5975)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5985..6728)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6126..6145)
                     /label="sCK131_Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          6329..6348
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     6371..6390
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(6425..6444)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(6729..6752)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(6752..6761)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(6781..6992)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(6993..7372)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7521..7540
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7532..7564
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 7565..7597
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7574..7592)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg agctcgtact ttgtaaactc
      421 cttctcgggg cgttatccaa atggcccgga ctatcagttg ctaaattatg gcagtggcag
      481 ctctctgagc ggctcttaca gggatcccgc tgccatgcac accggctctt acggctacaa
      541 ttacaatggg atggacctca gcgtcaaccg ctcctcggcc tcctccagcc actttggggc
      601 ggtgggcgag agctcgcgcg ccttccccgc gcccgcccag gagccccgct tcaggcaagc
      661 ggcttcgagc tgctccctgt cctcgcccga gtccctgccc tgcaccaacg gcgacagcca
      721 cggcgccaag ccctctgctt cgtccccctc cgaccaggcg acctcagcca gctccagcgc
      781 caatttcacc gaaatagacg aggccagcgc gtcctcggag cctgaggaag cggcaagcca
      841 gctaagcagc cccagcctag ctcgggcgca gccagagccc atggccacct ccacagccgc
      901 gcccgagggg cagactccgc aaatattccc ctggatgagg aagcttcaca tcagccatga
      961 tatgaccggg ccggacggga aaagggcccg gaccgcgtat acccgctacc agaccctgga
     1021 gctggaaaag gagttccact tcaaccgcta cctgacccgg cgacggcgca tcgagatcgc
     1081 ccacgcactc tgcctgtccg agcgccagat caagatctgg ttccagaacc ggcgcatgaa
     1141 gtggaagaag gacaacaaat tgaaaagtat gagcctggct acagctggca gcgccttcca
     1201 gcccTTGCCA aCTTTCTTGT ACAAAGTGGT ATACCCATAC GATGTTCCAG ATTACGCTta
     1261 aCTTTCTCCG CGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCAAAACTA
     1321 GAATGCAGTG AAAAAAATGC CTTATTTGTG AAATTTGTGA TGCTATTGCC TTATTTGTAA
     1381 CCATTATAAG CTGCAATAAA CAAGTTATCT ATAACAAGAA AATATATATA TAATAAGTTA
     1441 TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA TGAGTTAAAT CTTAAAAGTC
     1501 ACGTAAAAGA TAATCATGCG TCATTTTGAC TCACGCGGTC GTTATAGTTC AAAATCAGTG
     1561 ACACTTACCG CATTGACAAG CACGCCTCAC GGGAGCTCCA AGCGGCGACT GAGATGTCCT
     1621 AAATGCACAG CGACGGATTC GCGCTATTTA GAAAGAGAGA GCAATATTTC AAGAATGCAT
     1681 GCGTCAATTT TACGCAGACT ATCTTTCTAG GGTTAAGAAT TCACTGGCCG TCGTTTTACA
     1741 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC
     1801 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG
     1861 CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT
     1921 TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA
     1981 GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC
     2041 CGCTTACAGA CAAGCTGTGA CCATGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG
     2101 TCATCACCGA AACACGCGAG TTCGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA
     2161 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     2221 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     2281 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     2341 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     2401 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     2461 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     2521 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     2581 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     2641 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT
     2701 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
     2761 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT
     2821 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC
     2881 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
     2941 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
     3001 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGTTCA CGCGGTATCA TTGCAGCACT
     3061 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
     3121 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA
     3181 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     3241 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     3301 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     3361 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     3421 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     3481 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     3541 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     3601 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     3661 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     3721 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     3781 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     3841 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     3901 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     3961 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     4021 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG
     4081 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAT GAGCGCCCAA TACGCAAACC
     4141 GCCTCTCACC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG
     4201 GAAAGCGGGC AGTGAGCGCA ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     4261 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     4321 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
     4381 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
     4441 GCCAAGGTCG ACTTAACCCT AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA
     4501 TGCGTAAAAT TGACGCATGT GTTTTATCGG TCTGTATATC GAGGTTTATT TATTAATTTG
     4561 AATAGATATT AAGTTTTATT ATATTTACAC TTACATACTA ATAATAAATT CAACAAACAA
     4621 TTTATTTATG TTTATTTATT TATTAAAAAA AAACAAAAAC TCAAAATTTC TTCTATAAAG
     4681 TAACAAAACT TTTAGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT
     4741 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT
     4801 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA
     4861 TGTGGTATGG CTGATTATGA TCCTCTGGAG ATctattcct ttgccctcgg acgagtgctg
     4921 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     4981 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     5041 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     5101 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     5161 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     5221 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     5281 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     5341 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     5401 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     5461 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     5521 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
     5581 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     5641 cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc
     5701 gggagcgcgg ccgatgcaaa gtgccgatGa acataacgat ctttgtagaa accatcggcg
     5761 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     5821 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     5881 ttctcgacag acgtcgcggt gagttcaggc tttttcatGG GGCCGGGGTT CTCCTCCACG
     5941 TCGCCGGCCT GCTTCAGCAG GCTGAAGTTG GTGGCGCCGC TGCCCCCGGG GAGCATGTCA
     6001 AGGTCAAAAT CGTCAAGAGC GTCAGCAGGC AGCATATCAA GGTCAAAGTC GTCAAGGGCA
     6061 TCGGCTGGGA GCATGTCTAA GTCAAAATCG TCAAGGGCGT CGGTCGGCCC GCCGCTTTCG
     6121 CACTTTAGCT GTTTCTCCAG GCCACATATG ATTAGTTCCA GGCCGAAAAG GAAGGCAGGT
     6181 TCGGCTCCCT GCCGGTCGAA CAGCTCAATT GCTTGTTTCA GAAGTGGGGG CATAGAATCG
     6241 GTGGTAGGTG TCTCTCTTTC CTCTTTTGCT ACTTGATGCT CCTGTTCCTC CAATACGCAG
     6301 CCCAGTGTAA AGTGGCCCAC GGCGGACAGA GCGTACAGTG CGTTCTCCAG GGAGAAGCCT
     6361 TGCTGACACA GGAACGCGAG CTGATTTTCC AGGGTTTCGT ACTGTTTCTC TGTTGGGCGG
     6421 GTGCCGAGAT GCACTTTAGC CCCGTCACGA TGTGAGAGGA GAGCACAGCG GTATGACTTG
     6481 GCGTTGTTCC GCAGAAAGTC TTGCCATGAC TCGCCTTCCA GGGGGCAGGA GTGGGTATGA
     6541 TGCCTGTCCA GCATCTCGAT TGGCAGGGCA TCGAGCAGGG CCCGCTTGTT CTTCACGTGC
     6601 CAGTACAGGG TAGGCTGCTC AACTCCCAGC TTTTGAGCGA GTTTCCTTGT CGTCAGGCCT
     6661 TCGATACCGA CTCCATTGAG TAATTCCAGA GCAGAGTTTA TGACTTTGCT CTTGTCCAGT
     6721 CTAGACATCT TATCGTCATC GTCTTTGTAA TCCATGGTGG CGGATCCCGC GTCACGACAC
     6781 CTGTGTTCTG GCGGCAAACC CGTTGCGAAA AAGAACGTTC ACGGCGACTA CTGCACTTAT
     6841 ATACGGTTCT CCCCCACCCT CGGGAAAAAG GCGGAGCCAG TACACGACAT CACTTTCCCA
     6901 GTTTACCCCG CGCCACCTTC TCTAGGCACC GGTTCAATTG CCGACCCCTC CCCCCAACTT
     6961 CTCGGGCACT GTGGGCCATG TGCGCTCTGC CCCATGGTAA TAGCCATGAC TAATACGTAG
     7021 ATGTACTGCC AAGTAGGAAA GTGCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC
     7081 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT
     7141 GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTGCCTATT
     7201 GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT
     7261 CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA
     7321 ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG TCATAAGCTC
     7381 TAATCGCATA ATCGAGCTAA GAGCGATTAA TATGCTTAGC TAGCTTAGCT AAGCTAGATT
     7441 TCTCTATTCT CCCACAATAT ATAGCAGCTA TTAGCTTAGC AGTCCGAATA CGATCGTCCA
     7501 CGATCCATAA GCGTAACACA TTTGAGTAGC GACTGACGGG CACCGGAGCC ACTCGAGTGG
     7561 AATTACTGAC GGGCACCGGA GCCACTCGAG TGGAATT
//