LOCUS       CK530-H_TF137_STRA8     7930 bp ds-DNA     circular     13-JUL-2023
DEFINITION  .
COMMENT     Sequence created by bulk assembly. Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA Insert 5' end ligation method:
            homology with bases TTTGTACAAAA 3' end ligation method: homology
            with bases TTTCTTGTACAAAGT Backbone 5' end ligation method:
            homology with bases TTTCTTGTACAAAGT 3' end ligation method:
            homology with bases TTTGTACAAAA
FEATURES             Location/Qualifiers
     extracted re... 1..383
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          322..342
                     /label="mCMV_seq"
                     /note="sequence: GCTGTTTTGACCTCCATAGAA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(322..344)
                     /label="MPS467_EF1a-mTagBFP2-Gibson"
                     /note="sequence: TTCCTTTATGAGTTCACTCATGGTGGCtcttctatggaggtcaaaacagc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          348..367
                     /label="MPS324_mCMV_fwd2"
                     /note="sequence: CGGGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          350..367
                     /label="Universal_Primer_Forward"
                     /note="sequence: gtgctgtctcatcattttggcaaagGACCGATCCAGCCTACC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    368..383
                     /label="attR1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    368..392
                     /label="attB1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     attR1           369..383
                     /label="attR1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          372..391
                     /label="Genewiz_ATTB1"
                     /note="sequence: GTTTGTACAAAAAAGCAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(373..376)
                     /label="attL1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    373..392
                     /label="attB1_fragment"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    387..397
                     /label="Kozak?"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    398..1537
                     /label="STRA8"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             398..1537
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MATPEENSNPHDRATPQLPAQLQELEHRVARRRLSQARHRATLAALFNNLRKTVYSQSDLIASKWQVLNKAKSHIPELEQTLDNLLKLKASFNLEDGHASSLEEVKKEYASMYSGNDSLLSNSFPQNGSSPWCPTEAVRKDAEEEEDEEEEDQEEEEEEEEEEEEEEEEEEEEEEEEEKKVILYSPGTLSPDLMEFERYLNFYKQTMDLLTGSGIITPQEAALPIVSAAISHLWQNLSEERKASLRQAWAQKHRGPATLAEACREPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEVPSTSSSSSVLASCNPENPEEKFQLYMQIINFFKGLSCANTQVKQEASFPVDEEMIMLQCTETFDDEDL*"
     CDS             398..1594
                     /label="Translation 398-1594"
                     /translation="MATPEENSNPHDRATPQLPAQLQELEHRVARRRLSQARHRATLAALFNNLRKTVYSQSDLIASKWQVLNKAKSHIPELEQTLDNLLKLKASFNLEDGHASSLEEVKKEYASMYSGNDSLLSNSFPQNGSSPWCPTEAVRKDAEEEEDEEEEDQEEEEEEEEEEEEEEEEEEEEEEEEEKKVILYSPGTLSPDLMEFERYLNFYKQTMDLLTGSGIITPQEAALPIVSAAISHLWQNLSEERKASLRQAWAQKHRGPATLAEACREPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEVPSTSSSSSVLASCNPENPEEKFQLYMQIINFFKGLSCANTQVKQEASFPVDEEMIMLQCTETFDDEDL*LDPAFLYKVVYPYDVPDYA"
     misc_feature    1542..1563
                     /label="attB2_fragment"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1542..1565)
                     /label="Genewiz_ATTB2"
                     /note="sequence: CCACTTTGTACAAGAAAGCTGGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1542..1566)
                     /label="attB2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1545..1563)
                     /label="attP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     attR2           complement(1549..1565)
                     /label="attR2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     protein_bind    complement(1549..1566)
                     /label="attR2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     extracted re... 1549..1594
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1554..1563
                     /label="attL2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1560..1614
                     /label="MPS505_barcode_deletion_Gibson"
                     /note="sequence: aaGTGGTATACCCATACGATGTTCCAGATTACGCTCTTTCTCCGCGATCCAGACA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1568..1594
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1568..1594)
                     /label="MPS323_HA_rev"
                     /note="sequence: AGCGTAATCTGGAACATCGTATGGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1573..1589)
                     /label="Universal_Primer_Reverse"
                     /note="sequence: catatgactagtccccgaagctttTAAGCGTAATCTGGAACATCG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     motif           1595..1595
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     extracted re... 1595..1613
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1595..1614
                     /label="MPS505_barcode_deletion_Gibson"
                     /note="sequence: aaGTGGTATACCCATACGATGTTCCAGATTACGCTCTTTCTCCGCGATCCAGACA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1595..1630
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="LSPRSRHDKIH*"
     primer          complement(1601..1622)
                     /label="MPS294_barcode_rev2"
                     /note="sequence: TCTTATCATGTCTGGATCGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    1605..1739
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     extracted re... 1614..1769
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     extracted re... 1770..4480
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   complement(2011..2045)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2057..2072)
                     /label="M13F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2057..2072)
                     /label="Genewiz_M13F"
                     /note="sequence: GTAAAACGACGGCCAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2076..2092)
                     /label="Genewiz_M13-40FOR"
                     /note="sequence: GTTTTCCCAGTCACGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2281..2300)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     2402..2424
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2402..2424
                     /label="Genewiz_3GEX"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2463..2481)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2537..2556
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        2549..2653
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2654..3514
                     /label="AmpR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(2872..2891)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2872..2891)
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3685..4273
                     /label="ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     4174..4193
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4427..4444
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     extracted re... 4481..4569
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     extracted re... 4570..7864
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    4650..4671
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        4686..4716
                     /label="lac promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    4724..4740
                     /label="lac operator"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4731..4750
                     /label="Genewiz_M13-48REV"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     4748..4764
                     /label="M13R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4790..4852
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    5028..5109
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     5136..5155
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5143..5159
                     /label="Genewiz_EGFP-C-REV"
                     /note="sequence: GTTCAGGGGGAGGTGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5190..5209
                     /label="Genewiz_pFastBacR"
                     /note="sequence: CAAATGTGGTATGGCTGATT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5196..5245
                     /label="MPS317_HygR_rev"
                     /note="sequence: TGGTATGGCTGATTATGATCCTCTGGAGATctattcctttgccctcggac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(5202..5225)
                     /label="MPS318_resistance_rev"
                     /note="sequence: ATCTCCAGAGGATCATAATCAGCC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          5226..5245
                     /label="MPS317_HygR_rev"
                     /note="sequence: TGGTATGGCTGATTATGATCCTCTGGAGATctattcctttgccctcggac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(5226..6251)
                     /label="HygR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(5226..6251)
                     /label="Translation 6251-5226"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
     primer          complement(6231..6275)
                     /label="MPS316_P2A-HygR"
                     /note="sequence: GACGTGGAGGAGAACCCCGGCCCCatgaaaaagcctgaactcacc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          6252..6269
                     /label="MPS315_P2A-rev"
                     /note="sequence: GGGGCCGGGGTTCTCCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6252..6308)
                     /label="P2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6318..7061)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6459..6478)
                     /label="sCK131_Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          6662..6681
                     /label="qMPS015F"
                     /note="sequence: GAGCGTACAGTGCGTTCTCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     6704..6723
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(6758..6777)
                     /label="qMPS015R"
                     /note="sequence: CGGGGCTAAAGTGCATCTCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(7062..7085)
                     /label="FLAG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      complement(7085..7094)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(7114..7325)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      complement(7326..7705)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7854..7873
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     extracted re... 7865..7897
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     extracted re... 7898..7930
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(7907..7925)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTCCACCatg gcaacccctg aagaaaacag
      421 caatccccat gacagagcaa caccccagct gccagcacag ctgcaggagc ttgagcatcg
      481 ggtggcccgg agacggctgt cccaggcccg ccaccgagcc accctggcag cgctcttcaa
      541 caacctcagg aagacagtgt actctcagtc tgatctcata gcctcaaagt ggcaggttct
      601 gaataaggca aagagtcata ttccagaact ggagcaaacc ctggataatt tgctgaagct
      661 gaaagcatcc ttcaacctgg aagatgggca tgcaagcagc ttagaggagg tcaagaaaga
      721 atatgccagc atgtattctg gaaatgacag cctgctttca aacagttttc ctcagaatgg
      781 ttcctcccct tggtgcccaa ctgaggcagt caggaaggat gctgaggagg aggaagatga
      841 ggaagaggaa gatcaagaag aagaggagga ggaagaagaa gaggaggagg aggaagagga
      901 ggaagaggaa gaggaggagg aggaagagga gaaaaaagtg atcttatact ccccaggaac
      961 tttgtcgcct gacctcatgg aatttgaacg gtatctcaac ttttacaaac agacgatgga
     1021 ccttctgact ggcagcggga tcattacccc gcaggaggcg gcgctgccca tcgtctccgc
     1081 ggccatctcc cacctgtggc agaacctctc ggaggagagg aaggccagcc tccggcaggc
     1141 ctgggcgcag aagcaccgcg gccctgcgac cctggcggag gcctgccgag agccggcctg
     1201 tgccgagggc agcgtgaagg acagcggcgt ggacagccag ggggccagct gctcgctggt
     1261 ctccacgccc gaggagatcc tttttgagga tgcctttgat gtggcaagct tcctggacaa
     1321 aagtgaggtt ccgagtacat ctagctccag ttcagtgctt gccagctgca acccagaaaa
     1381 cccagaggag aagtttcagc tctatatgca gatcatcaac ttttttaaag gccttagctg
     1441 tgcaaacact caagtaaagc aggaagcatc ctttcccgtt gatgaagaga tgatcatgtt
     1501 gcagtgcaca gagacctttg acgatgaaga tttgtaaTTG GACCCAGCTT TCTTGTACAA
     1561 AGTGGTATAC CCATACGATG TTCCAGATTA CGCTCTTTCT CCGCGATCCA GACATGATAA
     1621 GATACATTGA TGAGTTTGGA CAAACCAAAA CTAGAATGCA GTGAAAAAAA TGCCTTATTT
     1681 GTGAAATTTG TGATGCTATT GCCTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
     1741 TCTATAACAA GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA
     1801 TATAATTATC GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT
     1861 GACTCACGCG GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT
     1921 CACGGGAGCT CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT
     1981 TTAGAAAGAG AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC
     2041 TAGGGTTAAG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT
     2101 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
     2161 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT
     2221 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG
     2281 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA
     2341 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCATGTC
     2401 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACACGC GAGTTCGAAA
     2461 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC
     2521 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
     2581 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     2641 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
     2701 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
     2761 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
     2821 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
     2881 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
     2941 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
     3001 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     3061 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     3121 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     3181 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     3241 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     3301 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     3361 TGAGCGTGGT TCACGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     3421 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     3481 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
     3541 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
     3601 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     3661 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     3721 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     3781 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     3841 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     3901 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     3961 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     4021 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     4081 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     4141 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     4201 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     4261 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     4321 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
     4381 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
     4441 CGAGGAAGCG GATGAGCGCC CAATACGCAA ACCGCCTCTC ACCGCGCGTT GGCCGATTCA
     4501 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAA
     4561 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC
     4621 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC
     4681 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA
     4741 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGG TCGACTTAAC CCTAGAAAGA
     4801 TAATCATATT GTGACGTACG TTAAAGATAA TCATGCGTAA AATTGACGCA TGTGTTTTAT
     4861 CGGTCTGTAT ATCGAGGTTT ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA
     4921 CACTTACATA CTAATAATAA ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA
     4981 AAAAAACAAA AACTCAAAAT TTCTTCTATA AAGTAACAAA ACTTTTAGCA GTGAAAAAAA
     5041 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT
     5101 AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG
     5161 GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCCTCTG
     5221 GAGATctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     5281 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     5341 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     5401 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     5461 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     5521 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     5581 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     5641 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     5701 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     5761 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     5821 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     5881 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     5941 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     6001 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     6061 tGaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     6121 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     6181 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     6241 ggctttttca tGGGGCCGGG GTTCTCCTCC ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG
     6301 TTGGTGGCGC CGCTGCCCCC GGGGAGCATG TCAAGGTCAA AATCGTCAAG AGCGTCAGCA
     6361 GGCAGCATAT CAAGGTCAAA GTCGTCAAGG GCATCGGCTG GGAGCATGTC TAAGTCAAAA
     6421 TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT TCGCACTTTA GCTGTTTCTC CAGGCCACAT
     6481 ATGATTAGTT CCAGGCCGAA AAGGAAGGCA GGTTCGGCTC CCTGCCGGTC GAACAGCTCA
     6541 ATTGCTTGTT TCAGAAGTGG GGGCATAGAA TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT
     6601 GCTACTTGAT GCTCCTGTTC CTCCAATACG CAGCCCAGTG TAAAGTGGCC CACGGCGGAC
     6661 AGAGCGTACA GTGCGTTCTC CAGGGAGAAG CCTTGCTGAC ACAGGAACGC GAGCTGATTT
     6721 TCCAGGGTTT CGTACTGTTT CTCTGTTGGG CGGGTGCCGA GATGCACTTT AGCCCCGTCA
     6781 CGATGTGAGA GGAGAGCACA GCGGTATGAC TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT
     6841 GACTCGCCTT CCAGGGGGCA GGAGTGGGTA TGATGCCTGT CCAGCATCTC GATTGGCAGG
     6901 GCATCGAGCA GGGCCCGCTT GTTCTTCACG TGCCAGTACA GGGTAGGCTG CTCAACTCCC
     6961 AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG CCTTCGATAC CGACTCCATT GAGTAATTCC
     7021 AGAGCAGAGT TTATGACTTT GCTCTTGTCC AGTCTAGACA TCTTATCGTC ATCGTCTTTG
     7081 TAATCCATGG TGGCGGATCC CGCGTCACGA CACCTGTGTT CTGGCGGCAA ACCCGTTGCG
     7141 AAAAAGAACG TTCACGGCGA CTACTGCACT TATATACGGT TCTCCCCCAC CCTCGGGAAA
     7201 AAGGCGGAGC CAGTACACGA CATCACTTTC CCAGTTTACC CCGCGCCACC TTCTCTAGGC
     7261 ACCGGTTCAA TTGCCGACCC CTCCCCCCAA CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC
     7321 TGCCCCATGG TAATAGCCAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTGCCAT
     7381 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG
     7441 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC
     7501 TCCACCCATT GACGTCAATG GAAAGTGCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT
     7561 ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT
     7621 TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT
     7681 TAATAACTAG TCAATAATCA ATGTCATAAG CTCTAATCGC ATAATCGAGC TAAGAGCGAT
     7741 TAATATGCTT AGCTAGCTTA GCTAAGCTAG ATTTCTCTAT TCTCCCACAA TATATAGCAG
     7801 CTATTAGCTT AGCAGTCCGA ATACGATCGT CCACGATCCA TAAGCGTAAC ACATTTGAGT
     7861 AGCGACTGAC GGGCACCGGA GCCACTCGAG TGGAATTACT GACGGGCACC GGAGCCACTC
     7921 GAGTGGAATT
//