LOCUS       pcDNA5-FRT-TO-FL        8896 bp DNA     circular SYN 04-JAN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5-FRT-TO-FLAG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    987..992
                     /label=kozac+Met
                     /note="color: #ccffff"
     CDS             993..1016
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    1040
                     /label=in frame
                     /note="color: #a6acb3"
     CDS             1041..4785
                     /codon_start=1
                     /label=BRIP1 cDNA
                     /note="color: #a6acb3"
                     /translation="SSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLES
                     PTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTS
                     RHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPL
                     ETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQ
                     GNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHT
                     CVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELV
                     SLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIED
                     CARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVER
                     DYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPV
                     ISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNK
                     KRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLE
                     ANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFL
                     PSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDG
                     ALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPG
                     RQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHS
                     TFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTPPYLLEAASHLSP
                     ENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTS
                     PTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPF
                     TDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLV
                     SEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILA
                     KDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKT
                     HEIEIKNFKPSPSKNKGMFPGFK"
     misc_feature    1041..1074
                     /label=anneal
                     /note="color: #a6acb3"
     misc_feature    1070..1099
                     /label=F1
                     /note="color: #ccffcc"
     misc_feature    1439..1464
                     /label=R6
                     /note="color: #ffcc99"
     misc_feature    1681..1708
                     /label=F2
                     /note="color: #ccffcc"
     misc_feature    2088..2115
                     /label=R5
                     /note="color: #ffcc99"
     misc_feature    2362..2389
                     /label=F3
                     /note="color: #ccffcc"
     misc_feature    2707..2735
                     /label=R4
                     /note="color: #ffcc99"
     misc_feature    3026..3054
                     /label=F4
                     /note="color: #ccffcc"
     misc_feature    3344..3367
                     /label=R3
                     /note="color: #ffcc99"
     misc_feature    3675..3699
                     /label=F5
                     /note="color: #ccffcc"
     misc_feature    3994..4018
                     /label=R2
                     /note="color: #ffcc99"
     misc_feature    4287..4314
                     /label=F6
                     /note="color: #ccffcc"
     misc_feature    4601..4630
                     /label=R1
                     /note="color: #ffcc99"
     misc_feature    4749..4785
                     /label=anneal
                     /note="color: #ccffff"
     polyA_signal    4854..5078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    5362..5409
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: RIGHT"
     CDS             5417..6439
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6569..6690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6739..6755)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6763..6779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6787..6817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6787 .. 6793 / #ffffff / -10
                        2: 6794 .. 6811 / #ffffff
                        3: 6812 .. 6817 / #ffffff / -35"
     protein_bind    6832..6853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7141..7729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7900..8760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7900 .. 8691 / #ccffcc
                        2: 8692 .. 8760 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8761..8865)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac gataaagccg
     1021 ccagtttaaa cggcgcgccg tcttcaatgt ggtctgaata tacaattggt ggggtgaaga
     1081 tttactttcc ttataaagct tacccgtcac agcttgctat gatgaattct attctcagag
     1141 gattaaacag caagcaacat tgtttgttgg agagtcccac aggaagtgga aaaagcttag
     1201 ccttactttg ttctgcttta gcatggcaac aatctcttag tgggaaacca gcagatgagg
     1261 gcgtaagtga aaaagctgaa gtacaattgt catgttgttg tgcatgccat tcaaaggatt
     1321 ttacaaacaa tgacatgaac caaggaactt cacgtcattt caactatcca agcacaccac
     1381 cttctgaaag aaatggcact tcatcaactt gtcaagactc ccctgaaaaa accactctgg
     1441 ctgcaaagtt atctgctaag aaacaggcat ccatatacag agatgaaaat gatgattttc
     1501 aagtagagaa gaaaagaatt cgacccttag aaactacaca gcagattaga aaacgtcatt
     1561 gctttggaac agaagtacac aatttggatg caaaagttga ttcaggaaag actgtaaaac
     1621 tcaactctcc actggaaaag ataaactcct tttcgccaca gaaaccccct ggccactgtt
     1681 ctaggtgctg ttgttctact aaacaaggaa acagtcaaga gtcatcgaat accattaaga
     1741 aggatcatac agggaaatcc aagataccca aaatatattt tgggacacgc acacacaagc
     1801 agattgctca gattactaga gagctccgga ggacggcata ttcaggggtt ccaatgacta
     1861 ttctttccag cagggatcat acttgtgtcc atcctgaggt agtcggtaac ttcaacagaa
     1921 atgagaagtg catggaattg ctagatggga aaaacggaaa atcctgctat ttttatcatg
     1981 gagttcataa aattagtgat cagcacacat tacagacttt ccaagggatg tgcaaagcct
     2041 gggatataga agaacttgtc agcctgggga agaaactaaa ggcctgtcca tattacacag
     2101 cccgagaact aatacaagat gctgacatca tattttgtcc ctacaactat cttctagatg
     2161 cacaaataag ggaaagtatg gatttaaatc tgaaagaaca ggttgtcatt ttagatgaag
     2221 ctcataacat cgaggactgt gctcgggaat cagcaagtta cagtgtaaca gaagttcagc
     2281 ttcggtttgc tcgggatgaa ctagatagta tggtcaacaa taatataagg aagaaagatc
     2341 atgaacccct acgagctgtg tgctgtagcc tcattaattg gttagaagca aacgctgaat
     2401 atcttgtaga aagagattat gaatcagctt gtaaaatatg gagtggaaat gaaatgctct
     2461 taactttaca caaaatgggt atcaccactg ctacttttcc cattttgcag ggacattttt
     2521 ctgctgttct tcaaaaagag gaaaaaatct caccaattta tggtaaagag gaggcaagag
     2581 aagtacctgt tattagtgca tcaactcaaa taatgcttaa aggacttttt atggtacttg
     2641 actatctttt taggcaaaat agcagatttg cagatgatta taaaattgcg attcaacaga
     2701 cttactcctg gacaaatcag attgatattt cagacaaaaa tgggttgttg gttctaccaa
     2761 aaaataagaa acgttcacga cagaaaactg cagttcatgt gctaaacttt tggtgcttaa
     2821 atccagctgt ggccttttca gatattaatg gcaaagttca gaccattgtt ttgacatctg
     2881 gtacattatc accaatgaaa tccttttcgt cagaacttgg tgttacattt actatccagc
     2941 tggaggctaa tcatatcatt aaaaattcac aggtttgggt tggtaccatt gggtcaggcc
     3001 ccaagggtcg gaatctctgt gctaccttcc agaatactga aacatttgag ttccaagatg
     3061 aagtgggagc acttttgtta tctgtgtgcc agactgtgag ccaaggaatt ttgtgtttct
     3121 tgccatctta caagttatta gaaaaattaa aagaacgttg gctctctact ggtttatggc
     3181 ataatctgga gttggtgaag acagtcattg tagaaccaca gggaggagaa aaaacaaatt
     3241 ttgatgaatt actgcaggtg tactatgacg caatcaaata caaaggagag aaagatggag
     3301 ctctcctggt agcagtttgt cgtggtaaag tgagtgaggg tctggatttc tcagatgaca
     3361 atgcccgtgc tgtcataaca ataggaattc cttttccaaa tgtgaaagat ctacaggttg
     3421 aactaaaacg acaatacaat gaccaccatt caaaattgag aggtcttcta cctggccgtc
     3481 agtggtatga aattcaagca tacagggcct taaaccaggc ccttggtaga tgtattagac
     3541 acagaaatga ttggggagct cttattctag tggatgatcg ctttaggaat aacccaagtc
     3601 gctatatatc tggactttct aaatgggtac ggcagcagat tcagcaccat tcaacctttg
     3661 aaagtgcact ggagtccttg gctgaatttt ccaaaaagca tcaaaaagtt cttaatgtat
     3721 ccataaagga cagaaccaat atacaggaca atgagtctac acttgaagtg acctctttaa
     3781 agtacagtac cccaccttat ttactggaag cagcaagtca tctatcacca gaaaattttg
     3841 tggaagatga agcaaagata tgtgtccagg aactacagtg tcctaaaatt attaccaaaa
     3901 attcacctct accaagtagc attatctcca gaaaggagaa aaatgatcca gtattcctgg
     3961 aagaagcagg gaaagcagaa aaaattgtga tttccagatc cacaagccca actttcaaca
     4021 aacaaacaaa gagagttagc tggtcaagct ttaattcttt gggacagtat tttactggta
     4081 aaataccgaa ggcaacacct gagctcgggt catcagagaa tagtgcctct agtcctcccc
     4141 gtttcaaaac agagaagatg gaaagtaaaa ctgttttgcc cttcactgat aaatgtgaat
     4201 cctcaaatct gacagtaaac acatcgtttg gatcatgccc tcaatcagaa accattattt
     4261 catcattaaa gattgatgcc acccttacta gaaaaaatca ttctgaacat ccgctctgtt
     4321 ctgaagaagc cctggatcca gacattgaat tgtctctagt aagtgaagaa gataaacagt
     4381 ccacttcaaa tagagatttt gaaacagaag cagaagatga atctatctat tttacacctg
     4441 aactttacga tcctgaagat acagatgaag aaaaaaatga cctagctgaa actgatagag
     4501 gaaatagatt ggctaacaat tcagattgca ttttagctaa agaccttttt gaaattagaa
     4561 ctataaaaga agtagattca gccagagaag tgaaagctga ggattgcata gatacaaagt
     4621 tgaatggaat tctgcatatt gaagaaagta aaattgatga cattgatggt aatgtaaaaa
     4681 caacttggat aaatgaactg gaactgggaa aaactcatga aatagaaata aagaacttta
     4741 aaccatctcc ttccaaaaat aaaggcatgt ttcctggttt taagtgagat atccatcaca
     4801 ctggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5041 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5101 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5341 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     5401 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     5461 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     5521 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     5581 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     5641 agtgcttgac attggggatt tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     5701 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     5761 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     5821 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     5881 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     5941 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6001 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6061 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6121 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6181 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6241 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6301 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6361 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6421 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     6481 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6541 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6601 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6661 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     6721 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6781 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6841 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6901 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8881 agtgccacct gacgtc
//
