LOCUS       pcDNA5-FRT-TO-FL        7877 bp DNA     circular SYN 11-JUN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    DD5063 pcDNA5-FRT/TO-Flag-CIP2A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7877
                     /mol_type="other DNA"
                     /note="/dnas_title=pcDNA5-FRT-TO-FLAG-CIP2A pDD5063"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #a6acb3"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tetracycline repressor TetR binding site
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/dnas_title=tetO"
                     /note="color: #31849b"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tetracycline repressor TetR binding site
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="/dnas_title=tetO"
                     /note="color: #31849b"
     CDS             990..3756
                     /codon_start=1
                     /label=FLAG-CIP2A
                     /note="FLAG-CIP2A"
                     /note="color: #993366"
                     /translation="MDYKDDDDKAASLNGAPMDSTACLKSLLLTVSQYKAVKSEANATQ
                     LLRHLEVISGQKLTRLFTSNQILTSECLSCLVELLEDPNISASLILSIIGLLSQLAVDI
                     ETRDCLQNTYNLNSVLAGVVCRSSHTDSVFLQCIQLLQKLTYNVKIFYSGANIDELITF
                     LIDHIQSSEDELKMPCLGLLANLCRHNLSVQTHIKTLSNVKSFYRTLITLLAHSSLTVV
                     VFALSILSSLTLNEEVGEKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKN
                     PKIADYLTRYEHFSSCLHQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFEQ
                     SPPGSATLGSHTKCLEPTVALLRWLSQPLDGSENCSVLALELFKEIFEDVIDAANCSSA
                     DRFVTLLLPTILDQLQFTEQNLDEALTRKKCERIAKAIEVLLTLCGDDTLKMHIAKILT
                     TVKCTTLIEQQFTYGKIDLGFGTKVADSELCKLAADVILKTLDLINKLKPLVPGMEVSF
                     YKILQDPRLITPLAFALTSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQ
                     ETEHIPRKMPWQSSNHSFPTSIKCLTPHLKDGVPGLNIEELIEKLQSGMVVKDQICDVR
                     ISDIMDVYEMKLSTLASKESRLQDLLETKALALAQADRLIAQHRCQRTQAETEARTLAS
                     MLREVERKNEELSVLLKAQQVESERAQSDIEHLFQHNRKLESVAEEHEILTKSYMELLQ
                     RNESTEKKNKDLQITCDSLNKQIETVKKLNESLKEQNEKSIAQLIEKEEQRKEVQNQLV
                     DREHKLANLHQKTKVQEEKIKTLQKEREDKEETIDILRKELSRTEQIRKELSIKASSLE
                     VQKAQLEGRLEEKESLVKLQQEELNKHSHMIAMIHSLSGGKINPETVNLSI"
     CDS             993..1016
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an
                     enterokinase cleavage site"
                     /label=FLAG(R) epitope tag, followed by an
                     enterokinas
                     /note="FLAG"
                     /note="/dnas_title=FLAG(R) epitope tag, followed by an
                     enterokinase cleavage site"
                     /note="color: #993366"
                     /translation="DYKDDDDK"
     insert          1041..3756
                     /direction=RIGHT
                     /codon_start=1
                     /note="/source=KIAA1524 (CIP2A)"
                     /note="/type=Custom cloned insert"
                     /note="color: #a6acb3"
                     /translation="MDSTACLKSLLLTVSQYKAVKSEANATQLLRHLEVISGQKLTRLF
                     TSNQILTSECLSCLVELLEDPNISASLILSIIGLLSQLAVDIETRDCLQNTYNLNSVLA
                     GVVCRSSHTDSVFLQCIQLLQKLTYNVKIFYSGANIDELITFLIDHIQSSEDELKMPCL
                     GLLANLCRHNLSVQTHIKTLSNVKSFYRTLITLLAHSSLTVVVFALSILSSLTLNEEVG
                     EKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKNPKIADYLTRYEHFSSCL
                     HQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFEQSPPGSATLGSHTKCLEP
                     TVALLRWLSQPLDGSENCSVLALELFKEIFEDVIDAANCSSADRFVTLLLPTILDQLQF
                     TEQNLDEALTRKKCERIAKAIEVLLTLCGDDTLKMHIAKILTTVKCTTLIEQQFTYGKI
                     DLGFGTKVADSELCKLAADVILKTLDLINKLKPLVPGMEVSFYKILQDPRLITPLAFAL
                     TSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQETEHIPRKMPWQSSNHS
                     FPTSIKCLTPHLKDGVPGLNIEELIEKLQSGMVVKDQICDVRISDIMDVYEMKLSTLAS
                     KESRLQDLLETKALALAQADRLIAQHRCQRTQAETEARTLASMLREVERKNEELSVLLK
                     AQQVESERAQSDIEHLFQHNRKLESVAEEHEILTKSYMELLQRNESTEKKNKDLQITCD
                     SLNKQIETVKKLNESLKEQNEKSIAQLIEKEEQRKEVQNQLVDREHKLANLHQKTKVQE
                     EKIKTLQKEREDKEETIDILRKELSRTEQIRKELSIKASSLEVQKAQLEGRLEEKESLV
                     KLQQEELNKHSHMIAMIHSLSGGKINPETVNLSI"
     primer_bind     1556..1575
                     /label=Primer 1
                     /note="color: black; sequence: CAATCTTTCTGTTCAAACGC"
     misc_RNA        1998..2017
                     /label=guide RNA#3
                     /note="guide RNA#3"
                     /note="color: #a6acb3; direction: RIGHT"
     PCR_primer      2088..2106
                     /note="color: #a6acb3"
     misc_feature    2147..2164
                     /note="color: #a6acb3"
     primer_bind     2647..2668
                     /label=Primer 2
                     /note="color: black; sequence: CTCCACTGCCAGATTTTCCTGC"
     misc_RNA        2992..3011
                     /label=guide RNA #2
                     /note="guide RNA #2"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        3647..3666
                     /label=guide RNA #1
                     /note="guide RNA #1"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    3835..4059
                     /label=bGH poly(A) signal
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    4343..4390
                     /label=FLP recombinase from the Saccharomyces
                     cerevisi
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b"
     CDS             4398..5420
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=aph(4)-Ia
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /note="/dnas_title=HygR"
                     /note="color: #993366"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5550..5671
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5720..5736)
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5744..5760
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5768..5798)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    5813..5834
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6122..6710)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/dnas_title=ori"
                     /note="color: #ffff00"
     CDS             complement(6881..7741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="/dnas_title=AmpR"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7742..7846)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="/dnas_title=AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggactacaa agacgatgac gataaagccg
     1021 ccagtttaaa cggcgcgcca atggactcca ctgcctgctt gaagtccttg ctcctgactg
     1081 tcagtcagta caaagccgtg aagtcagagg cgaacgccac tcagcttttg cggcacttgg
     1141 aggtaatttc tggacagaaa ctcacacgac tatttacatc aaatcagata ttaacaagtg
     1201 aatgcttgag ttgccttgta gagctacttg aagaccccaa cataagtgct tcactgatct
     1261 taagtattat cggtttgctg tctcaactag cagtagacat tgaaaccaga gattgtcttc
     1321 agaatacata taatctgaat agtgtgctgg cgggagtggt ttgtcggagc agccacactg
     1381 attcggtgtt tttgcagtgc attcaacttc tacagaagtt aacatataat gtcaaaattt
     1441 tctattctgg tgccaatata gatgaattaa ttacgttcct gatagatcac attcaatctt
     1501 ctgaagatga gttaaaaatg ccttgtctag gattattggc aaatctttgt cggcacaatc
     1561 tttctgttca aacgcacata aagacattga gtaatgtgaa atctttttat cgaactctta
     1621 tcaccttgtt ggcccatagt agtttaactg tggttgtgtt tgcactttca atattatcca
     1681 gtttgacatt aaatgaagag gtgggggaaa agctattcca tgctcgaaac attcatcaga
     1741 cttttcaact aatatttaat attctcataa acggtgatgg cactctaact agaaagtatt
     1801 cagttgacct actgatggat ctccttaaga atcctaaaat tgctgattat ctcaccagat
     1861 atgagcactt ttcttcatgt cttcaccaag tattaggtct tcttaatgga aaggatcctg
     1921 attcctcttc aaaggtttta gaattacttc ttgccttctg ttcagtgact cagctgcgcc
     1981 atatgctcac tcagatgatg tttgaacagt ctccacctgg cagcgccact ctgggaagcc
     2041 atactaaatg tttagaacct actgtggctc tactgcgctg gttaagccaa cctttggacg
     2101 gatcagaaaa ctgttctgtt ttagcattgg agttgttcaa ggaaatattt gaggatgtca
     2161 tagatgctgc taactgttcc tcggctgatc gttttgtgac ccttctgctg cctacaatcc
     2221 ttgatcaact tcagttcaca gaacaaaatc tagatgaggc tttaacaaga aaaaaatgtg
     2281 aaaggattgc caaggccatt gaagttttgt taactctctg tggagatgat acactaaaaa
     2341 tgcatattgc aaaaatcttg acaactgtca agtgtaccac tcttatagaa caacaattta
     2401 catatggcaa gattgacctg ggatttggaa caaaggttgc agattctgaa ttatgcaaac
     2461 ttgctgctga tgtaattttg aaaactcttg atttgattaa caaacttaaa ccattggttc
     2521 ctggtatgga agtaagcttc tacaaaatac ttcaggaccc acgtttgatt actcctttgg
     2581 cttttgcttt aacgtcagat aatagagaac aagtacagtc tggactgaga atattattgg
     2641 aggctgctcc actgccagat tttcctgctt tagtacttgg agaaagtata gcagcaaaca
     2701 atgcctatag acaacaggaa acagaacata tacccagaaa aatgccctgg caatcatcaa
     2761 atcacagttt tccaacatca ataaagtgtt taactcctca tttgaaagat ggtgttcctg
     2821 gattgaatat tgaagaatta atagagaaac ttcagtctgg aatggtggta aaggatcaga
     2881 tttgtgatgt gagaatatct gacataatgg atgtatatga aatgaaacta tccacattag
     2941 cttccaaaga aagcaggcta caagatcttt tggaaacaaa agctctagcc cttgcacagg
     3001 ctgatagact gattgctcag catcgctgtc aaagaactca agctgaaaca gaggcacgga
     3061 cacttgctag tatgttgaga gaagttgaga gaaaaaatga agagcttagt gtgttgctga
     3121 aggcgcagca agttgaatca gaaagagcgc agagtgatat tgagcatctc tttcaacata
     3181 ataggaagtt agagtctgtg gctgaagaac atgaaatact gacaaaatcc tacatggaac
     3241 ttcttcagag aaatgaaagt actgaaaaga agaataaaga tttacagatc acatgtgatt
     3301 ctctgaataa acaaattgag acagtgaaaa agttgaatga gtcactcaag gaacaaaatg
     3361 aaaaaagtat tgcccaatta atagagaaag aagaacagag aaaagaagta cagaatcagc
     3421 tagtagacag agaacataag ctagcaaatt tgcatcaaaa aacaaaagta caagaagaaa
     3481 agattaaaac cttacaaaag gaaagggaag ataaggaaga aaccattgat atccttagaa
     3541 aagaattaag cagaacagaa cagataagaa aagagttgag cattaaggct tcctccctag
     3601 aggttcaaaa ggcacaatta gaaggtcgtt tggaagagaa agagtccttg gtgaaacttc
     3661 agcaagagga attgaacaaa cactcccaca tgatagcaat gatccacagt ttaagtggtg
     3721 gaaaaataaa tccagaaact gtgaatctca gtatataaga attctgcaga tatccatcac
     3781 actggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     3841 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3901 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3961 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4021 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4081 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4141 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4201 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4261 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4321 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta
     4381 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     4441 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     4501 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     4561 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     4621 aagtgcttga cattggggat ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     4681 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     4741 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     4801 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     4861 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     4921 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     4981 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5041 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5101 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     5161 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     5221 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     5281 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     5341 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     5401 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg
     5461 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5521 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5581 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5641 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     5701 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5761 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5821 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5881 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5941 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6001 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6061 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6121 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6241 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6301 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6361 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6421 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6481 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6541 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6601 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6781 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6841 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6901 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6961 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7021 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7081 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7141 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7201 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7261 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7321 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7381 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7441 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7501 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7561 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7621 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7681 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7741 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7801 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7861 aagtgccacc tgacgtc
//
