LOCUS       AT1R                    7643 bp    DNA     linear       20-OCT-2021
FEATURES             Location/Qualifiers
     misc_feature    1325..1348
                     /note="FLAG"
     misc_feature    1277..1324
                     /note="HA signal sequence"
     misc_feature    4843..5443
                     /note="puro resistance"
     misc_feature    1352..2425
                     /note="AT1R"
     CDS             join(2462..3076,3077..3460)
                     /dnas_title="tTA"
                     /note="In the Tet-Off%C2%AE system, tTA binds to the
                     Tet-responsive element%A0and stimulates transcription
                     only in the absence of tetracycline or doxycycline."
                     /transl_table=1
                     /codon_start=1
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGS
                     TIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAM
                     AHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
                     /product="tetracycline-controlled transactivator,
                     comprising a fusion of the tetracycline repressor TetR
                     with the C-terminal activation domain of herpes simplex
                     virus VP16"
     misc_feature    2438..2458
                     /note="TEV site"
     misc_feature    2459..3463
                     /note="tTa"
     source          1..7643
                     /dnas_title="AT1R TTA pTarget Puro"
ORIGIN      
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG
      661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
      721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC
      781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT
      841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA
      901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT
      961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC
     1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAAAATTG GATCTCCATT CGCCATTCAG
     1081 GCTGCGCAAC TGCTGGGAAG GACGATCAGA GCGGGCCTCT TCGCTATTAC GCCAGCTGGC
     1141 GAAAGGGACG TGGCAAGCAA GGCGATTAAG TTGAGTTACG CCAGGATTTT CCCAGTCACG
     1201 ACGTTGTAAA ACGACGGCCA GAGAATTATA ATACGACTCA CTATAGGGCG AATTCGGATC
     1261 CTTGCTAGCC GCCACCATGA AGACGATCAT CGCCCTGAGC TACATCTTCT GCCTGGTATT
     1321 CGCCGACTAC AAGGACGATG ATGACGCCGC CATTCTCAAC TCTTCTACTG AAGATGGTAT
     1381 TAAAAGAATC CAAGATGATT GTCCCAAAGC TGGAAGGCAT AATTACATAT TTGTCATGAT
     1441 TCCTACTTTA TACAGTATCA TCTTTGTGGT GGGAATATTT GGAAACAGCT TGGTGGTGAT
     1501 AGTCATTTAC TTTTATATGA AGCTGAAGAC TGTGGCCAGT GTTTTTCTTT TGAATTTAGC
     1561 ACTGGCTGAC TTATGCTTTT TACTGACTTT GCCACTATGG GCTGTCTACA CAGCTATGGA
     1621 ATACCGCTGG CCCTTTGGCA ATTACCTATG TAAGATTGCT TCAGCCAGCG TCAGTTTCAA
     1681 CCTGTACGCT AGTGTGTTTC TACTCACGTG TCTCAGCATT GATCGATACC TGGCTATTGT
     1741 TCACCCAATG AAGTCCCGCC TTCGACGCAC AATGCTTGTA GCCAAAGTCA CCTGCATCAT
     1801 CATTTGGCTG CTGGCAGGCT TGGCCAGTTT GCCAGCTATA ATCCATCGAA ATGTATTTTT
     1861 CATTGAGAAC ACCAATATTA CAGTTTGTGC TTTCCATTAT GAGTCCCAAA ATTCAACCCT
     1921 CCCGATAGGG CTGGGCCTGA CCAAAAATAT ACTGGGTTTC CTGTTTCCTT TTCTGATCAT
     1981 TCTTACAAGT TATACTCTTA TTTGGAAGGC CCTAAAGAAG GCTTATGAAA TTCAGAAGAA
     2041 CAAACCAAGA AATGATGATA TTTTTAAGAT AATTATGGCA ATTGTGCTTT TCTTTTTCTT
     2101 TTCCTGGATT CCCCACCAAA TATTCACTTT TCTGGATGTA TTGATTCAAC TAGGCATCAT
     2161 ACGTGACTGT AGAATTGCAG ATATTGTGGA CACGGCCATG CCTATCACCA TTTGTATAGC
     2221 TTATTTTAAC AATTGCCTGA ATCCTCTTTT TTATGGCTTT CTGGGGAAAA AATTTAAAAG
     2281 ATATTTTCTC CAGCTTCTAA AATATATTCC CCCAAAAGCC AAATCCCACT CAAACCTTTC
     2341 AACAAAAATG AGCACGCTTT CCTACCGCCC CTCAGATAAT GTAAGCTCAT CCACCAAGAA
     2401 GCCTGCACCA TGTTTTGAGG TTGAGacttc atcgaccggt gagaacctgt acttccagct
     2461 aagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     2521 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     2581 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     2641 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     2701 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     2761 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     2821 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     2881 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     2941 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     3001 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     3061 atgtgaaagt gggtccgcgt acagccgcgc gcgtacgaaa aacaattacg ggtctaccat
     3121 cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc tggcggctcc
     3181 gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc ccccgaccga
     3241 tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga
     3301 cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt
     3361 taccccccac gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca
     3421 gatgtttacc gatgcccttg gaattgacga gtacggtggg TAATGGTACC GTCGACTGCG
     3481 GCCGCGAATT CCAAGCTTGA GTATTCTATC GTGTCACCTA AATAACTTGG CGTAATCATG
     3541 GTCATATCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
     3601 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
     3661 GTTGCGCGAT GCTTCCATTT TGTGAGGGTT AATGCTTCGA GAAGACATGA TAAGATACAT
     3721 TGATGAGTTT GGACAAACCA CAACAAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT
     3781 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA
     3841 CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTAAAGCAA
     3901 GTAAAACCTC TACAAATGTG GTAAAATCCG ATAAGGATCG ATTCCGGAGC CTGAATGGCG
     3961 AATGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCACGTG
     4021 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC
     4081 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA
     4141 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT
     4201 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT
     4261 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT
     4321 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
     4381 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT CGCCTGTGTA CCTTCTGAGG
     4441 CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC
     4501 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC
     4561 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT
     4621 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
     4681 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
     4741 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTGA
     4801 TTCTTCTGAC ACAACAGTCT CGAACTTAAG GCTAGAGCCA CCATGACCGA GTACAAGCCC
     4861 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
     4921 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
     4981 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
     5041 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
     5101 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
     5161 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
     5221 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
     5281 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
     5341 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
     5401 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGCGGGACT CTGGGGTTCG
     5461 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGATG GCCGCAATAA AATATCTTTA
     5521 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAAGATC CGCGTATGGT GCACTCTCAG
     5581 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA
     5641 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC
     5701 CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG
     5761 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC
     5821 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA
     5881 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
     5941 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
     6001 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     6061 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     6121 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     6181 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     6241 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     6301 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     6361 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     6421 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     6481 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     6541 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     6601 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     6661 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     6721 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     6781 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     6841 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     6901 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     6961 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     7021 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     7081 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
     7141 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     7201 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     7261 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     7321 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     7381 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     7441 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     7501 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     7561 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     7621 TGCTCACATG GCTCGACAGA TCT
//

