LOCUS       FLAG-AT1R               6111 bp    DNA     linear       30-NOV-2022
FEATURES             Location/Qualifiers
     misc_feature    235..243
                     /note=""
     misc_feature    2118..2135
                     /note="BGHREV"
     promoter        310..817
                     /note="CMV promoter"
     misc_feature    768..788
                     /note="CMV Fwd Primer"
     gene            5115..5975
                     /note="Amp resistance"
     operon          818..837
                     /note="Tet Operator"
     operon          839..858
                     /note="Tet Operator"
     TATA_signal     803..809
                     /note="TATA"
     misc_feature    932..979
                     /note="HA Signal Sequence"
     misc_feature    980..1003
                     /note="FLAG"
     misc_feature    926..931
                     /note="Kozak"
     misc_feature    1004..2080
                     /note="AT1R"
     misc_feature    1718..1725
                     /note=""
     misc_feature    1643..1660
                     /note=""
     source          1..6111
                     /dnas_title="FLAG-AT1R pCDNA TZ"
ORIGIN      
        1 ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC AGTACAATCT GCTCTGATGC
       61 CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC
      121 GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC AATTGCATGA AGAATCTGCT
      181 TAGGGTTAGG CGTTTTGCGC TGCTTCGCGA TGTACGGGCC AGATATACGC GTTGACATTG
      241 ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT
      301 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC
      361 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA
      421 TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA
      481 TCATATGCCA AGTCCGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA
      541 TGCCCAGTAC ATGACCTTAC GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT
      601 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACACCAAT GGGCGTGGAT AGCGGTTTGA
      661 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA
      721 AAATCAACGG GACTTTCCAA AATGTCGTAA TAACCCCGCC CCGTTGACGC AAATGGGCGG
      781 TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCTC CCTATCAGTG ATAGAGATCT
      841 CCCTATCAGT GATAGAGATC GTCGACGAGC TCGTTTAGTG AACCGTCAGA TCTCTAGAAG
      901 CTGGGTACCA GCTGCTAGCA AGCTTGCCAC CATGAAGACG ATCATCGCCC TGAGCTACAT
      961 CTTCTGCCTG GTGTTCGCCG ACTACAAGGA CGATGATGAC AAAATTCTCA ACTCTTCTAC
     1021 TGAAGATGGT ATTAAAAGAA TCCAAGATGA TTGTCCCAAA GCTGGAAGGC ATAATTACAT
     1081 ATTTGTCATG ATTCCTACTT TATACAGTAT CATCTTTGTG GTGGGAATAT TTGGAAACAG
     1141 CTTGGTGGTG ATAGTCATTT ACTTTTATAT GAAGCTGAAG ACTGTGGCCA GTGTTTTTCT
     1201 TTTGAATTTA GCACTGGCTG ACTTATGCTT TTTACTGACT TTGCCACTAT GGGCTGTCTA
     1261 CACAGCTATG GAATACCGCT GGCCCTTTGG CAATTACCTA TGTAAGATTG CTTCAGCCAG
     1321 CGTCAGTTTC AACCTGTACG CTAGTGTGTT TCTACTCACG TGTCTCAGCA TTGATCGATA
     1381 CCTGGCTATT GTTCACCCAA TGAAGTCCCG CCTTCGACGC ACAATGCTTG TAGCCAAAGT
     1441 CACCTGCATC ATCATTTGGC TGCTGGCAGG CTTGGCCAGT TTGCCAGCTA TAATCCATCG
     1501 AAATGTATTT TTCATTGAGA ACACCAATAT TACAGTTTGT GCTTTCCATT ATGAGTCCCA
     1561 AAATTCAACC CTTCCGATAG GGCTGGGCCT GACCAAAAAT ATACTGGGTT TCCTGTTTCC
     1621 TTTTCTGATC ATTCTTACAA GTTATACTCT TATTTGGAAG GCCCTAAAGA AGGCTTATGA
     1681 AATTCAGAAG AACAAACCAA GAAATGATGA TATTTTTAAG ATAATTATGG CAATTGTGCT
     1741 TTTCTTTTTC TTTTCCTGGA TTCCCCACCA AATATTCACT TTTCTGGATG TATTGATTCA
     1801 ACTAGGCATC ATACGTGACT GTAGAATTGC AGATATTGTG GACACGGCCA TGCCTATCAC
     1861 CATTTGTATA GCTTATTTTA ACAATTGCCT GAATCCTCTT TTTTATGGCT TTCTGGGGAA
     1921 AAAATTTAAA AGATATTTTC TCCAGCTTCT AAAATATATT CCCCCAAAAG CCAAATCCCA
     1981 CTCAAACCTT TCAACAAAAA TGAGCACGCT TTCCTACCGC CCCTCAGATA ATGTAAGCTC
     2041 ATCCACCAAG AAGCCTGCAC CATGTTTTGA GGTTGAGTGA CTCGAGTCTA GAGGGCCCGT
     2101 TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC
     2161 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA
     2221 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG
     2281 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG
     2341 CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC
     2401 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT
     2461 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC
     2521 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT
     2581 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC
     2641 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT
     2701 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT
     2761 TTTGGGGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA
     2821 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGGCAGG
     2881 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG
     2941 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC
     3001 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
     3061 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT
     3121 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC
     3181 TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT
     3241 ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC
     3301 GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC
     3361 GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC
     3421 CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG
     3481 GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG
     3541 GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC
     3601 CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG
     3661 CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC
     3721 CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC
     3781 CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
     3841 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
     3901 TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG
     3961 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
     4021 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
     4081 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
     4141 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     4201 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     4261 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     4321 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     4381 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     4441 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     4501 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC
     4561 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     4621 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     4681 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     4741 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
     4801 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     4861 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     4921 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     4981 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     5041 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     5101 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     5161 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     5221 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
     5281 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     5341 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     5401 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     5461 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     5521 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     5581 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     5641 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     5701 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     5761 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     5821 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     5881 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     5941 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     6001 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     6061 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C
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