LOCUS       pDonor2-FIGLA-mGreenLan 8212 bp ds-DNA     circular     09-JAN-2021
DEFINITION  .
KEYWORDS    "creator:Anastasiya Sybirna" "marker:AmpR"
FEATURES             Location/Qualifiers
     CDS             complement(171..962)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          794..813
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(963..1031)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1032..1136)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1120..1172
                     /label="MPS206_FIGLA_5'_gibson"
                     /note="sequence: caaataggggttccgcgcacatttccccgTCACTTGTATAGTGGAAAGACTCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1129..1148)
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1207..1228
                     /label="MPS203_FIGLA_geno_5'"
                     /note="sequence: ttttgtgtcagagactgctcct"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1219..1238
                     /label="MPS191_FIGLA_5'_Gibson"
                     /note="sequence: caaataggggttccgcgcacatttccccggactgctcctggcagtgtat"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            1913..1947
                     /label="FIGLA-001 Exon 4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1962..1981
                     /label="MPS204_FIGLA_geno_5'_inner"
                     /note="sequence: aacctgatgcatagtgccaa"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2191..2214)
                     /label="FIGLA_cterm"
                     /note="sequence: TACTTGTGGAAGTCTGAAACAGAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2191..2250)
                     /label="MPS192_FIGLA_T2A_Gibson"
                     /note="sequence: gcatgttagcagACTTCCTCTGCCCTCGCCAGAGCCTACTTGTGGAAGTCTGAAACAGAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     linker          2215..2223
                     /label="GSG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2215..2234
                     /label="MPS114_GSG-T2A_fwd"
                     /note="sequence: GGCTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2215..2277
                     /label="Translation 2215-2277"
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     primer          2224..2244
                     /label="MPS105_T2A_Gibson"
                     /note="sequence: GAGGGCAGAGGAAGTctgcta"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2224..2277
                     /label="T2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2234..2253)
                     /label="MPS106_T2A_Gibson_rev"
                     /note="sequence: accgcatgttagcagACTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2278..2297)
                     /label="MPS086_FP_rev_seq"
                     /note="sequence: TCCTCGCCCTTGCTCACCAT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2278..2997
                     /label="mGreenLantern"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2278..2997
                     /label="Translation 2278-2997"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIKANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLKERVTAAGITHDMDELYK*"
     primer          complement(2960..2981)
                     /label="MPS039_BsaI_FP_3'_rev"
                     /note="sequence: ATAGGTCTCcATGCcgagagtgatcccgg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2971..2994
                     /label="MPS038_BsaI_FP_3'_fwd"
                     /note="sequence: ATCGGTCTCgGCATggacgagctgtacaag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2976..2997
                     /label="MPS178_FP_3'"
                     /note="sequence: catggacgagctgtacaagtaa"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2976..2997)
                     /label="MPS179_FP_3'"
                     /note="sequence: ttacttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     recombinatio... 2998..3029
                     /label="Rox"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3036..3060
                     /label="MPS026_PGK_fwd"
                     /note="sequence: AGAAAGTATAGGAACTTCAGCTTGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3041..3065)
                     /label="MPS027_PGK_5'_rev"
                     /note="sequence: CGATATCAAGCTGAAGTTCCTATAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        3079..3578
                     /label="PGK promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3167..3167
                     /label="G->C"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3580..3605)
                     /label="MPS074_PGK_3'_BsaI"
                     /note="sequence: ATTGGTCTCaTGGTGGCGTTGGCTGCAGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3581..3600)
                     /label="MPS081_Resistance_marker_switching_5'"
                     /note="sequence: CATGGTGGCGTTGGCTGCAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3598..3617
                     /label="MPS047_Puro_fwd_seq"
                     /note="sequence: ATGGGtACCGAGTACAAGCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3598..5199
                     /label="PuroTK"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3598..5199
                     /label="Translation 3598-5199"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAGSMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN*"
     primer          4195..4214
                     /label="MPS045_TK_fwd"
                     /note="sequence: GCCGGATCCATGCCCACGCT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(5180..5199)
                     /label="MPS046_TK_rev"
                     /note="sequence: TCAGTTAGCCTCCCCCATCT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          5200..5222
                     /label="MPS082_Resistance_marker_switching_3'"
                     /note="sequence: GCTCTAGAGCTCGCTGATCAGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5227..5451
                     /label="polyA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     recombinatio... complement(5499..5530)
                     /label="Rox"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          5513..5571
                     /label="MPS193_FIGLA_Rox_Gibson"
                     /note="sequence: gccaattatttaaagttaGGCGCGCCGATATCTAGCAGGGTTACCTTCTAGAGACAAAT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(5527..5547)
                     /label="MPS113_Rox_rev"
                     /note="sequence: CTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5531..5546
                     /label="spacer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          5548..5571
                     /label="FIGLA_3'UTR_fwd"
                     /note="sequence: CAGGGTTACCTTCTAGAGACAAAT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(5574..5597)
                     /label="MPS205_FIGLA_geno_3'_inner"
                     /note="sequence: ACTCCAAAACTTTCAAGACTGCAT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(6300..6324)
                     /label="FIGLA_rev"
                     /note="sequence: actATGTCTTTGTGAGCAATGGTTA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6300..6352)
                     /label="MPS194_FIGLA_3'_Gibson"
                     /note="sequence: GAAAACCACACTGACAAGATCTGGAGGTactATGTCTTTGTGAGCAATGGTTA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          6325..6344
                     /label="MPS104_MC1_fwd"
                     /note="sequence: ACCTCCAGATCTTGTCAGTG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6340..6363
                     /label="primer 267"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Promoter        6340..6617
                     /label="MC1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(6588..6607)
                     /label="MPS131_MC1_rev_seq"
                     /note="sequence: TGCAGGGTCGCTCGGTGTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6618..7274
                     /label="DTA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6618..7275
                     /label="Translation 6618-7275"
                     /translation="MDPDDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSLCEGTLLLWCDIIGQTTYRDLKL*"
     misc_feature    7276..7341
                     /label="small T intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    7383..7620
                     /label="Mouse PGK poly(A)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          7591..7640
                     /label="MPS107_ori_PGKpA_Gibson"
                     /note="sequence: TTGCTTTATGATAATGTTTCATAGTTGgatgtttttccataggctccgcc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(7593..7620)
                     /label="MPS103_PGKpA_rev"
                     /note="sequence: atcCAACTATGAAACATTATCATAAAGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(7624..8212)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgTC ACTTGTATAG TGGAAAGACT CGATCAAGAT TAGaaaaaaa aaGCTACTCA
     1201 TACAtatttt gtgtcagaga ctgctcctgg cagtgtatac atattaacct atttaatttt
     1261 cacaaccacc ctgtgctata caaacaatta tatccatttt acatgtgagg aaactaagga
     1321 acagaagtaa cttgcccaat acctgcaagt cagtgaagtg ttggggttGG GGGGGATGTG
     1381 GGTGATAGTG TTAAGTCCTT TTTAACCACT TTCGTTCTAA CAAAACTCAA CTTTGAAATG
     1441 TAAAGGAGGG TGGACTAGAA AATAGATAAA TGTTTAAGGC TAAGTTAAAG CATTTGATTG
     1501 CGGTGCTTTA CCATCCACAG TGATGTACAT AATAGGTTTC AGTATCCAAA ATGCCTGACC
     1561 ATGACTTGAG CGGCTTTATT TTTCAGTCAT GGGTTTATTT TTTCTTCAAG TTGAGAGTTT
     1621 CAGAAAATTG AGCAGAGACA AAATGATCTT GCCACGTTAG TAAAACCACT GATGGAAATA
     1681 CACTAAAATC CTTCCCATTT CTTTTCCTTT GCAACTAAAA TAAAAAATTA TCATCAGGTA
     1741 CATAACATTG TGTGCCTGTT CTTGATTTTT CTATCACTGG CATGTTTATG TGACCCATTA
     1801 GGTGGGacaa tttttttact gttggccact atgttctcag ttttttgttc tcttaaacgt
     1861 gacaaataac cgatgtgttt agccctttct gtattttgta ttgtttttgc aggatagatt
     1921 cccagaagta gaactgctga gtcacaggta ggtcattcta taacctgatg catagtgcca
     1981 atttataaca ccactggctg tataataaga gtgctaattc catcacattc ttagcaatga
     2041 gctctattaa aagaaggaat cttaatgggt aaaaaagtag tatctttaaa tttaaacatc
     2101 tttgattact aataaaatta attttttgca tatttcttta tttcttttgt aaacggttct
     2161 tatttttaac tatttatAAT GGAATGTTTT CTCTGTTTCA GACTTCCACA AGTAGGCTCT
     2221 GGCGAGGGCA GAGGAAGTct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
     2281 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2341 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     2401 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2461 gtgaccacct taggctacgg cgtggcctgc ttcgcccgct accccgacca catgaagcag
     2521 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     2581 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2641 aaccgcatcg tgctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2701 ctggagtaca acttcaacag ccacaaggtc tatatcacgg ccgacaagca gaagaacggc
     2761 atcaaggcta acttcaagac ccgccacaac gttgaggacg gcggcgtgca gctcgccgac
     2821 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2881 ctgagccatc agtccaaact gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2941 aaggagaggg tgaccgccgc cgggattaca catgacatgg acgagctgta caagtaataa
     3001 ctttaaataa tgccaattat ttaaagttac atatgAGAAA GTATAGGAAC TTCAGCTTGA
     3061 TATCGAATTA ATTCTACCGG GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT GGAGCATGCG
     3121 CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT CGCACACATT
     3181 CCACATCCAC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC GCGCCACCTT
     3241 CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA GCTCGCGTCG TGCAGGACGT
     3301 GACAAATGGA AGTAGCACGT CTCACTAGTC TCGTGCAGAT GGACAGCACC GCTGAGCAAT
     3361 GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG
     3421 CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG
     3481 CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG CGCACGTCTG
     3541 CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC CTGCAGCCAA CGCCACCATG
     3601 GGGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA
     3661 CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC
     3721 CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC
     3781 ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG
     3841 AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT
     3901 TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG
     3961 CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC
     4021 AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG
     4081 GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC
     4141 GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCGGA
     4201 TCCATGCCCA CGCTACTGCG GGTTTATATA GACGGTCCTC ACGGGATGGG GAAAACCACC
     4261 ACCACGCAAC TGCTGGTGGC CCTGGGTTCG CGCGACGATA TCGTCTACGT ACCCGAGCCG
     4321 ATGACTTACT GGCAGGTGCT GGGGGCTTCC GAGACAATCG CGAACATCTA CACCACACAA
     4381 CACCGCCTCG ACCAGGGTGA GATATCGGCC GGGGACGCGG CGGTGGTAAT GACAAGCGCC
     4441 CAGATAACAA TGGGCATGCC TTATGCCGTG ACCGACGCCG TTCTGGCTCC TCATATCGGG
     4501 GGGGAGGCTG GGAGCTCACA TGCCCCGCCC CCGGCCCTCA CCCTCATCTT CGACCGCCAT
     4561 CCCATCGCCG CCCTCCTGTG CTACCCGGCC GCGCGATACC TTATGGGCAG CATGACCCCC
     4621 CAGGCCGTGC TGGCGTTCGT GGCCCTCATC CCGCCGACCT TGCCCGGCAC AAACATCGTG
     4681 TTGGGGGCCC TTCCGGAGGA CAGACACATC GACCGCCTGG CCAAACGCCA GCGCCCCGGC
     4741 GAGCGGCTTG ACCTGGCTAT GCTGGCCGCG ATTCGCCGCG TTTACGGGCT GCTTGCCAAT
     4801 ACGGTGCGGT ATCTGCAGGG CGGCGGGTCG TGGCGGGAGG ATTGGGGACA GCTTTCGGGG
     4861 ACGGCCGTGC CGCCCCAGGG TGCCGAGCCC CAGAGCAACG CGGGCCCACG ACCCCATATC
     4921 GGGGACACGT TATTTACCCT GTTTCGGGCC CCCGAGTTGC TGGCCCCCAA CGGCGACCTG
     4981 TACAACGTGT TTGCCTGGGC CTTGGACGTC TTGGCCAAAC GCCTCCGTCC CATGCACGTC
     5041 TTTATCCTGG ATTACGACCA ATCGCCCGCC GGCTGCCGGG ACGCCCTGCT GCAACTTACC
     5101 TCCGGGATGG TCCAGACCCA CGTCACCACC CCCGGCTCCA TACCGACGAT CTGCGACCTG
     5161 GCGCGCACGT TTGCCCGGGA GATGGGGGAG GCTAACTGAG CTCTAGAGCT CGCTGATCAG
     5221 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
     5281 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
     5341 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
     5401 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG
     5461 CGGAAAGAAC CAGCTGGGGC TCGACATACT AGagtactta actttaaata atgccaatta
     5521 tttaaagtta GGCGCGCCGA TATCTAGCAG GGTTACCTTC TAGAGACAAA TAAATGCAGT
     5581 CTTGAAAGTT TTGGAGTCTC TCtttttttt ttttttttga gatagagttt cactcttatc
     5641 acccaggctg gagtggcacg gtgtgatctc ggctcactgc aacctccgcc tcctgggttc
     5701 aagtgattct cctgcctcag cctcctgagt agctgggatt acaggcatgc gccaccacgc
     5761 ccagctaatt tttgtatttt tagtagagac agggcttctc cacgttggtc aggctggtct
     5821 cgaactcccg acctcaggtg attcaaccac ctcagcctcc caaagtgctg ggattacagg
     5881 aatgagccac tgtgcccCGT GGGAGTCTCT TTTTTAAAAA AGAAGTTTTG TAGTAATTCC
     5941 GAGTTTAACA ATTACCCTTT TAAATAGCCT TGCCTCTCTC AAGTTTACTC CACTCTGCCT
     6001 TCTCCTTAAC TTCATGCTTT GAAGAAATAG GCTTCAGGGG CTTCATCCTT TGCTTCCAAC
     6061 TCACTGTCAT CCTCTGGGGA CCTACATCTT TTTTTCATTG CTTTGAGAAA TAAGTGACTG
     6121 TTACCCTTCC AATAGAAATT AACCTTAATA GAAATGAAGA CTTAAGCTAA AAGAGTTGTA
     6181 AACATTGGCC AAGGGGAGTG AGAGTTTTCA AGGGTTTCTA GATACCCAGC TAAGGCCAAA
     6241 TGGCACCTTT TGTGCAAGGT AGACAGCCAA ACACCTTGAG CACAAAGACT TCGTCTTTAT
     6301 AACCATTGCT CACAAAGACA TagtACCTCC AGATCTTGTC AGTGTGGTTT TCAAGAGGAA
     6361 GCAAAAAGCC TCTCCACCCA GGCCTGGAAT GTTTCCACCC AATGTCGAGC AGTGTGGTTT
     6421 TGCAAGAGGA AGCAAAAAGC CTCTCCACCC AGGCCTGGAA TGTTTCCACC CAATGTCGAG
     6481 CAAACCCCGC CCAGCGTCTT GTCATTGGCG AATTCGAACA CGCAGATGCA GTCGGGGCGG
     6541 CGCGGTCCCA GGTCCACTTC GCATATTAAG GTGACGCGTG TGGCCTCGAA CACCGAGCGA
     6601 CCCTGCAGGT CCTCGCCATG GATCCTGATG ATGTTGTTGA TTCTTCTAAA TCTTTTGTGA
     6661 TGGAAAACTT TTCTTCGTAC CACGGGACTA AACCTGGTTA TGTAGATTCC ATTCAAAAAG
     6721 GTATACAAAA GCCAAAATCT GGTACACAAG GAAATTATGA CGATGATTGG AAAGGGTTTT
     6781 ATAGTACCGA CAATAAATAC GACGCTGCGG GATACTCTGT AGATAATGAA AACCCGCTCT
     6841 CTGGAAAAGC TGGAGGCGTG GTCAAAGTGA CGTATCCAGG ACTGACGAAG GTTCTCGCAC
     6901 TAAAAGTGGA TAATGCCGAA ACTATTAAGA AAGAGTTAGG TTTAAGTCTC ACTGAACCGT
     6961 TGATGGAGCA AGTCGGAACG GAAGAGTTTA TCAAAAGGTT CGGTGATGGT GCTTCGCGTG
     7021 TAGTGCTCAG CCTTCCCTTC GCTGAGGGGA GTTCTAGCGT TGAATATATT AATAACTGGG
     7081 AACAGGCGAA AGCGTTAAGC GTAGAACTTG AGATTAATTT TGAAACCCGT GGAAAACGTG
     7141 GCCAAGATGC GATGTATGAG TATATGGCTC AAGCCTGTGC AGGAAATCGT GTCAGGCGAT
     7201 CTCTTTGTGA AGGAACCTTA CTTCTGTGGT GTGACATAAT TGGACAAACT ACCTACAGAG
     7261 ATTTAAAGCT CTAAGGTAAA TATAAAATTT TTAAGTGTAT AATGTGTTAA ACTACTGATT
     7321 CTAATTGTTT GTGTATTTTA GATTCCAACC TATGGAACTG ATGAATGGGA GCAGTGGTGG
     7381 AATGCAGATC CACTAGAGTC GAGGGATGCA GAAATTGATG ATCTATTAAA CAATAAAGAT
     7441 GTCCACTAAA ATGGAAGTTT TTCCTGTCAT ACTTTGTTAA GAAGGGTGAG AACAGAGTAC
     7501 CTACATTTTG AATGGAAGGA TTGGAGCTAC GGGGGTGGGG GTGGGGTGGG ATTAGATAAA
     7561 TGCCTGCTCT TTACTGAAGG CTCTTTACTA TTGCTTTATG ATAATGTTTC ATAGTTGgat
     7621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8041 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aa
//