LOCUS       pDonor2-NPM2-mGreenLant 8523 bp ds-DNA     circular     02-MAR-2023
DEFINITION  .
KEYWORDS    "creator:Anastasiya Sybirna" "marker:AmpR"
FEATURES             Location/Qualifiers
     exon            921..954
                     /label="NPM2-001 Exon 8"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1018..1037
                     /label="MPS213_NPM2_5'_geno"
                     /note="sequence: GGTATGGAGAAGGGAACGGG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1085..1144)
                     /label="MPS210_NPM2_5'_T2A_Gibson"
                     /note="sequence: cgcatgttagcagACTTCCTCTGCCCTCGCCAGAGCCTTTCTTGAATCCTGGCTTCTTGG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1089..1107)
                     /label="NPM2-Cterm2 REV"
                     /note="sequence: aaacCATTTCTTGAATCCTGGCTTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     linker          1108..1116
                     /label="GSG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1108..1127
                     /label="MPS114_GSG-T2A_fwd"
                     /note="sequence: GGCTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1108..1137
                     /label="MPS078_DDX4_T2A_Gibson"
                     /note="sequence: CCCAATCCAGTAGATGATGAGTCATGGGATGGCTCTGGCGAGGGCAGAGGAAGTctgcta"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1108..1170
                     /label="Translation 1108-1170"
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     primer          1111..1127
                     /label="MPS470_BsaI-T2A-Gibson"
                     /note="sequence: agagaccattactgggtctccGGgTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1111..1127
                     /label="MPS476_GSG-T2A-fwd"
                     /note="sequence: GGgTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1111..1127
                     /label="MPS476_GSG-T2A-fwd"
                     /note="sequence: GGgTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1111..1127
                     /label="MPS470_BsaI-T2A-Gibson"
                     /note="sequence: agagaccattactgggtctccGGgTCTGGCGAGGGCAGAGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1111..1130
                     /label="MPS098_REC8-T2A-Gibson"
                     /note="sequence: CTCCTGATCCAGCCGGGGCCCAGATTCCACGGgTCTGGCGAGGGCAGAGGAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1111..1130
                     /label="MPS040_BsaI_T2A"
                     /note="sequence: ATAGGTCTCcggggggtctggCGAGGGCAGAGGAAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1111..1164
                     /label="MPS035_T2A_top"
                     /note="sequence: ggggggtctggcGAGGGCAGAGGAAGTctgctaacatgcggtgacgtcgaggagaatcct"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1117..1137
                     /label="MPS105_T2A_Gibson"
                     /note="sequence: GAGGGCAGAGGAAGTctgcta"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1117..1170
                     /label="T2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1127..1146)
                     /label="MPS106_T2A_Gibson_rev"
                     /note="sequence: accgcatgttagcagACTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1151..1170)
                     /label="deleted9"
                     /note="sequence: tgggccaggattctcctcga"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1151..1176)
                     /label="MPS461_T2A-SYCP3-Gibson"
                     /note="sequence: CCTGGAATACTTTTTTCCGGAGGACACCATtgggccaggattctcctcga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1151..1176)
                     /label="MPS461_T2A-SYCP3-Gibson"
                     /note="sequence: CCTGGAATACTTTTTTCCGGAGGACACCATtgggccaggattctcctcga"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1155..1173)
                     /label="T2A-rev-seq"
                     /note="sequence: CATtgggccaggattctcc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1163..1190
                     /label="MPS037_BsaI_T2A_FP"
                     /note="sequence: ATAGGTCTCtggcccaatggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1171..1190
                     /label="MPS043_BsaI_FP_5'"
                     /note="sequence: ATAGGTCTcCATGgtgagcaagggcgagga"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1171..1190
                     /label="MPS180_FP_5'_fwd"
                     /note="sequence: atggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1171..1190
                     /label="MPS031_BsaI_Linker_FP"
                     /note="sequence: ATAGGTCTCcggggggtctggtggcagtggagggggaagcatggtgagcaagggcgagga"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1171..1190)
                     /label="MPS086_FP_rev_seq"
                     /note="sequence: TCCTCGCCCTTGCTCACCAT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1171..1890
                     /label="mGreenLantern"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1171..1890
                     /label="Translation 1171-1890"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIKANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLKERVTAAGITHDMDELYK*"
     primer          1179..1199
                     /label="MPS459_SYCP3_mGL_Gibson"
                     /note="sequence: GTTTTTTAAAGTCAGGAAGCATCTAAATATGGTGTCCaagggcgaggagctgttcac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1179..1199
                     /label="MPS459_SYCP3_mGL_Gibson"
                     /note="sequence: GTTTTTTAAAGTCAGGAAGCATCTAAATATGGTGTCCaagggcgaggagctgttcac"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1210..1228
                     /label="MPS473_mGreenLantern_fwd"
                     /note="sequence: cccatcctggtcgagctgg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1210..1228
                     /label="MPS473_mGreenLantern_fwd"
                     /note="sequence: cccatcctggtcgagctgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1217..1237)
                     /label="Genewiz_EGFP-N"
                     /note="sequence: cgtcgccgtccagctcgacca"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1217..1237)
                     /label="Genewiz_EGFP-N"
                     /note="sequence: CGTCGCCGTCCAGCTCGACCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1217..1237)
                     /label="Genewiz_EGFP-N"
                     /note="sequence: CGTCGCCGTCCAGCTCGACCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1869..1887
                     /label="MPS038_BsaI_FP_3'_fwd"
                     /note="sequence: ATCGGTCTCgGCATggacgagctgtacaag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1869..1887)
                     /label="MPS487_mGreenLantern_T2A_Gibson"
                     /note="sequence: tcaccgcatgttagcagACTTCCTCTGCCCTCGCCAGAcCCcttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1869..1887)
                     /label="MPS487_mGreenLantern_T2A_Gibson"
                     /note="sequence: tcaccgcatgttagcagACTTCCTCTGCCCTCGCCAGAcCCcttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1869..1890
                     /label="MPS178_FP_3'"
                     /note="sequence: catggacgagctgtacaagtaa"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1869..1890)
                     /label="MPS471_GFP-SV40pA-Gibson"
                     /note="sequence: tcttatcatgtctggatcgcggagaaagcttacttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1869..1890)
                     /label="MPS471_GFP-SV40pA-Gibson"
                     /note="sequence: tcttatcatgtctggatcgcggagaaagcttacttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1869..1890)
                     /label="MPS179_FP_3'"
                     /note="sequence: ttacttgtacagctcgtccatg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     recombinatio... 1891..1922
                     /label="Rox"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1929..1953
                     /label="MPS026_PGK_fwd"
                     /note="sequence: AGAAAGTATAGGAACTTCAGCTTGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1934..1958)
                     /label="MPS027_PGK_5'_rev"
                     /note="sequence: CGATATCAAGCTGAAGTTCCTATAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        1972..2471
                     /label="PGK promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2406..2425
                     /label="PGK_seq"
                     /note="sequence: TTCTGCACGCTTCAAAAGCG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2473..2493)
                     /label="MPS074_PGK_3'_BsaI"
                     /note="sequence: ATTGGTCTCaTGGTGGCGTTGGCTGCAGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2473..2498)
                     /label="MPS074_PGK_3'_BsaI"
                     /note="sequence: ATTGGTCTCaTGGTGGCGTTGGCTGCAGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2474..2493)
                     /label="deleted8"
                     /note="sequence: agacgtcgcggtgagttcaggctttttcatGGTGGCGTTGGCTGCAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2474..2493)
                     /label="MPS081_Resistance_marker_switching_5'"
                     /note="sequence: CATGGTGGCGTTGGCTGCAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2491..2510
                     /label="MPS047_Puro_fwd_seq"
                     /note="sequence: ATGGGtACCGAGTACAAGCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2491..4092
                     /label="PuroTK"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2491..4092
                     /label="Translation 2491-4092"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAGSMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN*"
     primer          complement(2957..2975)
                     /label="MPS032_BsaI_PuroR_rev"
                     /note="sequence: ATTGGTCTCTTTCCAGGAAGGCGGGCACCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2977..2994
                     /label="MPS033_BsaI_PuroR_fwd"
                     /note="sequence: ATTGGTCTCTGGAAACCTCCGCGCCCCGCAAC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3088..3107
                     /label="MPS045_TK_fwd"
                     /note="sequence: GCCGGATCCATGCCCACGCT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4073..4092)
                     /label="MPS046_TK_rev"
                     /note="sequence: TCAGTTAGCCTCCCCCATCT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4090..4113
                     /label="Resistance_marker_switching_3'"
                     /note="sequence: TGAGCTCTAGAGCTCGCTGATCAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4093..4114
                     /label="HygR_Gibson_3'_fwd"
                     /note="sequence: cccagcactcgtccgagggcaaaggaatagGCTCTAGAGCTCGCTGATCAGC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          4093..4115
                     /label="MPS082_Resistance_marker_switching_3'"
                     /note="sequence: GCTCTAGAGCTCGCTGATCAGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          4093..4117
                     /label="deleted7"
                     /note="sequence: cccagcactcgtccgagggcaaaggaatagGCTCTAGAGCTCGCTGATCAGCCTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4114..4131)
                     /label="Genewiz_BGHR"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(4114..4131)
                     /label="Genewiz_BGHR"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    4120..4344
                     /label="polyA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          4341..4360
                     /label="polyA_seq"
                     /note="sequence: ATGGCTTCTGAGGCGGAAAG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4365..4385)
                     /label="MPS028_insert_rev"
                     /note="sequence: gcagcgagtcagtgagcgagGAGACCCTAGTATGTCGAGCCCCAGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4366..4385)
                     /label="MPS034_BsaI_Insert_3'_rev"
                     /note="sequence: AAAGGTCTCagcgagCTAGTATGTCGAGCCCCAGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     recombinatio... complement(4392..4423)
                     /label="Rox"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4406..4458
                     /label="MPS211_NPM2_3'_rox_Gibson"
                     /note="sequence: gccaattatttaaagttaGGCGCGCCGATATCTAGGGGGCACGGTGCAAAGTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4420..4440)
                     /label="MPS113_Rox_rev"
                     /note="sequence: CTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4420..4440)
                     /label="MPS095_Rox_SYCP1_Gibson_rev"
                     /note="sequence: AACTATAAGTTTCACGTTATTAGTCTCCTTCTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4420..4440)
                     /label="MPS032_Rox_DDX4_Gibson"
                     /note="sequence: ATTTTCTTCTCTGCATCAAAACCACAGACTCTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4420..4440)
                     /label="MPS077_Rox_DDX4_3'_Gibson"
                     /note="sequence: TATTTTCTTCTCTGCATCAAAACCACAGACTAGCTGGGTCTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4420..4442)
                     /label="MPS099_Rox_REC8_Gibson_rev"
                     /note="sequence: TCCTGGCAGCTTTGTAAATGGACTCTAACCCTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4420..4442)
                     /label="MPS060-Rox-REC8-Gibson"
                     /note="sequence: TGGCCGGTTTCCTGGCAGCTTTGTAAATGGACTCTAACCCTAGATATCGGCGCGCCtaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4424..4439
                     /label="spacer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          4440..4458
                     /label="NPM2_3'_fwd"
                     /note="sequence: GGGGGCACGGTGCAAAGTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4508..4527)
                     /label="MPS214_NPM2_3'_geno"
                     /note="sequence: TGCATTCAGACACAGGTGGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5478..5529)
                     /label="NPM2_3'_rev_inner"
                     /note="sequence: TTGAAAACCACACTGACAAGATCTGGAGGTCTAAGCAGGACAGCAGGACCAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5500..5519
                     /label="MPS104_MC1_fwd"
                     /note="sequence: ACCTCCAGATCTTGTCAGTG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          5500..5519
                     /label="MPS100_pBluescript-REC8_3'_Gibson"
                     /note="sequence: TTGGTACAGGCAAAATCTCTTCAGAGAGCAACCTCCAGATCTTGTCAGTG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          5500..5519
                     /label="MPS076_MC1_DDX4_3'_Gibson"
                     /note="sequence: ACCCAGTGAGGTGTAAATACTGGGGAGACAACCTCCAGATCTTGTCAGTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5506..5528
                     /label="deleted"
                     /note="sequence: ACCCAGTGAGGTGTAAATACTGGGGAGACAAGATCTTGTCAGTGTGGTTTTCA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          5506..5528
                     /label="MPS096_SYCP1_MC1_Gibson"
                     /note="sequence: ggcagattatgtagcactttaggccactgtAGATCTTGTCAGTGTGGTTTTCA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          5506..5528
                     /label="MPS049-REC8-3'-MC1-DTA-Gibson"
                     /note="sequence: TTGGTACAGGCAAAATCTCTTCAGAGAGCAAGATCTTGTCAGTGTGGTTTTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5515..5538
                     /label="primer 267"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     Promoter        5515..5792
                     /label="MC1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5763..5782)
                     /label="MPS131_MC1_rev_seq"
                     /note="sequence: TGCAGGGTCGCTCGGTGTTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5793..6449
                     /label="DTA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5793..6450
                     /label="Translation 5793-6450"
                     /translation="MDPDDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSLCEGTLLLWCDIIGQTTYRDLKL*"
     misc_feature    6451..6516
                     /label="small T intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    6558..6795
                     /label="Mouse PGK poly(A)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          6766..6815
                     /label="MPS107_ori_PGKpA_Gibson"
                     /note="sequence: TTGCTTTATGATAATGTTTCATAGTTGgatgtttttccataggctccgcc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6768..6795)
                     /label="MPS033_DTA_rev_seq"
                     /note="sequence: ggatcCAACTATGAAACATTATCATAAAGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6768..6795)
                     /label="MPS103_PGKpA_rev"
                     /note="sequence: atcCAACTATGAAACATTATCATAAAGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(6799..7387)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(7558..8349)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          8181..8200
                     /label="MPS101_Amp_rev"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(8221..8241)
                     /label="MPS474_AmpR_rev_Gibson"
                     /note="sequence: cccgaagaacgttttccaatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(8221..8241)
                     /label="AmpR_rev_gibson"
                     /note="sequence: cccgaagaacgttttccaatg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(8350..8418)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(8419..8523)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          8507..3
                     /label="AmpR_prom_seq"
                     /note="sequence: caaataggggttccgcgcac"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          8507..37
                     /label="MPS209_NPM2_5'_AmpR_Gibson"
                     /note="sequence: caaataggggttccgcgcacatttccccgAGTTCATACAGCAGGACATAAAGTC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(8516..12)
                     /label="MPS112_AmpR_prom_fwd"
                     /note="sequence: cggggaaatgtgcgcggaac"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 cacatttccc cgAGTTCATA CAGCAGGACA TAAAGTCATT TTTATTTTAT TTCACATATT
       61 TTTAAAAAGA GTTTGACCAg gccgggtacg gtggctcacg cctgtaatcc cagcactttg
      121 ggaagccaag gtgggaggat cacttgaggt caggagttca agaccagcct tgccaacata
      181 gcaaaaccct gtctctacta aaatacaaaa ttcagctggg cgtggtggca tgtgcctgta
      241 atcccagcta ctcgggaggc tgaggcagga gaatcactta gacccggaat gcgaaggatg
      301 cagtgaacca agatcacacc actgcacacc agcctgggca acagagcaag actccatctc
      361 aaaaaaaaaa aaaaaaGTTT GACCAAGAAA AAAATAATAA CCCTGAAAGA AAATACACCA
      421 AAATGTTTAG TGTGGGCAGT AAAGAAAACT ATAAGTAATG TATTTTCTTG TCTATTTGCT
      481 ATATTTTGTA CAAAATGGTT AATATTTTAT AATGAAAAAG ACATTTGTGG gccaggtgtg
      541 gtggtgcaca cctgcagtcc cagcttctca ggaggctgag gcaggaggat cactcgagcc
      601 caggaggtcg aggctgcagt gagctgtgat agtgtcactg cactccagcc tgggcaacag
      661 aaccagactc catctcaaaa acaaaacaaa acaaaaGACA TTTGTGATAA CTAAATGAAG
      721 ATGGAAGCCT AAGGAAAACA CATATGCGTA TGCATGCACA CGCACACACA TCCCTTTGTT
      781 TAAAGAGTCC GAGTGGTCCC CAGGAGGAGC AGCCAGGCTT GCTTTCCAGG GTGGGCACTG
      841 GGAGGGCCAC GCCGCTGGTC TGGAGCTGAG CTCTCTCCCT GACCCCAATC CCACTCCTGC
      901 TCCGCTCCAC CCTGTTGCAG AGCCAGCGTT AGAGACAAGA GCCCTGTGAA AAAGGTGAGT
      961 AGGACCAGAG GGCTTTGGCC CTTGGGACAG GCGAGTATTC TCTGGAGGGG GCTGCCTGGT
     1021 ATGGAGAAGG GAACGGGACC CTGGAGCCCT GCCTTCCCTC CACAGGCCAA AGCCACAGCC
     1081 AGAGCCAAGA AGCCAGGATT CAAGAAAGGC TCTGGCGAGG GCAGAGGAAG Tctgctaaca
     1141 tgcggtgacg tcgaggagaa tcctggccca atggtgagca agggcgagga gctgttcacc
     1201 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtc
     1261 cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
     1321 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttaggcta cggcgtggcc
     1381 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1441 gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta caagacccgc
     1501 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgtgctgaa gggcatcgac
     1561 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaag
     1621 gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa gacccgccac
     1681 aacgttgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1741 gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccaa actgagcaaa
     1801 gaccccaacg agaagcgcga tcacatggtc ctgaaggaga gggtgaccgc cgccgggatt
     1861 acacatgaca tggacgagct gtacaagtaa taactttaaa taatgccaat tatttaaagt
     1921 tacatatgAG AAAGTATAGG AACTTCAGCT TGATATCGAA TTAATTCTAC CGGGTAGGGG
     1981 AGGCGCTTTT CCCAAGGCAG TCTGGAGCAT GCGCTTTAGC AGCCCCGCTG GGCACTTGGC
     2041 GCTACACAAG TGGCCTCTGG CCTCGCACAC ATTCCACATC CACCGGTAGG CGCCAACCGG
     2101 CTCCGTTCTT TGGTGGCCCC TTCGCGCCAC CTTCTACTCC TCCCCTAGTC AGGAAGTTCC
     2161 CCCCCGCCCC GCAGCTCGCG TCGTGCAGGA CGTGACAAAT GGAAGTAGCA CGTCTCACTA
     2221 GTCTCGTGCA GATGGACAGC ACCGCTGAGC AATGGAAGCG GGTAGGCCTT TGGGGCAGCG
     2281 GCCAATAGCA GCTTTGCTCC TTCGCTTTCT GGGCTCAGAG GCTGGGAAGG GGTGGGTCCG
     2341 GGGGCGGGCT CAGGGGCGGG CTCAGGGGCG GGGCGGGCGC CCGAAGGTCC TCCGGAGGCC
     2401 CGGCATTCTG CACGCTTCAA AAGCGCACGT CTGCCGCGCT GTTCTCCTCT TCCTCATCTC
     2461 CGGGCCTTTC GACCTGCAGC CAACGCCACC ATGGGGACCG AGTACAAGCC CACGGTGCGC
     2521 CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC
     2581 TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG
     2641 CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC
     2701 GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC
     2761 GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG
     2821 GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC
     2881 GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG
     2941 GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC
     3001 TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC
     3061 ACCTGGTGCA TGACCCGCAA GCCCGGTGCC GGATCCATGC CCACGCTACT GCGGGTTTAT
     3121 ATAGACGGTC CTCACGGGAT GGGGAAAACC ACCACCACGC AACTGCTGGT GGCCCTGGGT
     3181 TCGCGCGACG ATATCGTCTA CGTACCCGAG CCGATGACTT ACTGGCAGGT GCTGGGGGCT
     3241 TCCGAGACAA TCGCGAACAT CTACACCACA CAACACCGCC TCGACCAGGG TGAGATATCG
     3301 GCCGGGGACG CGGCGGTGGT AATGACAAGC GCCCAGATAA CAATGGGCAT GCCTTATGCC
     3361 GTGACCGACG CCGTTCTGGC TCCTCATATC GGGGGGGAGG CTGGGAGCTC ACATGCCCCG
     3421 CCCCCGGCCC TCACCCTCAT CTTCGACCGC CATCCCATCG CCGCCCTCCT GTGCTACCCG
     3481 GCCGCGCGAT ACCTTATGGG CAGCATGACC CCCCAGGCCG TGCTGGCGTT CGTGGCCCTC
     3541 ATCCCGCCGA CCTTGCCCGG CACAAACATC GTGTTGGGGG CCCTTCCGGA GGACAGACAC
     3601 ATCGACCGCC TGGCCAAACG CCAGCGCCCC GGCGAGCGGC TTGACCTGGC TATGCTGGCC
     3661 GCGATTCGCC GCGTTTACGG GCTGCTTGCC AATACGGTGC GGTATCTGCA GGGCGGCGGG
     3721 TCGTGGCGGG AGGATTGGGG ACAGCTTTCG GGGACGGCCG TGCCGCCCCA GGGTGCCGAG
     3781 CCCCAGAGCA ACGCGGGCCC ACGACCCCAT ATCGGGGACA CGTTATTTAC CCTGTTTCGG
     3841 GCCCCCGAGT TGCTGGCCCC CAACGGCGAC CTGTACAACG TGTTTGCCTG GGCCTTGGAC
     3901 GTCTTGGCCA AACGCCTCCG TCCCATGCAC GTCTTTATCC TGGATTACGA CCAATCGCCC
     3961 GCCGGCTGCC GGGACGCCCT GCTGCAACTT ACCTCCGGGA TGGTCCAGAC CCACGTCACC
     4021 ACCCCCGGCT CCATACCGAC GATCTGCGAC CTGGCGCGCA CGTTTGCCCG GGAGATGGGG
     4081 GAGGCTAACT GAGCTCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC
     4141 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG
     4201 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC
     4261 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG
     4321 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCGACAT
     4381 ACTAGagtac ttaactttaa ataatgccaa ttatttaaag ttaGGCGCGC CGATATCTAG
     4441 GGGGCACGGT GCAAAGTGGG CCTTCCCTGG GCTGTGCTGC AGGCACAGGG TGCCCCTGTC
     4501 CAGCCCCTCC ACCTGTGTCT GAATGCAACA GGGGTGTTGC GGGGGCAACA TGAGAGCCCC
     4561 TCACCCCCAA CTCTCCACTT TCAGGAGGCC CCCAGTGAAG AGCCCCACCT CGGGGTCACA
     4621 ATAAAGTTGC CTGGTCAGGA CTTTCCTTCT CTTCCCTGGA GCCAGCCTCC TTGTCCGCTG
     4681 CACCAGCCCC AGTGCCCGGC AGAGGGCAGC CTTGAACCGG TGCACCCGGG CCCTGAGGTC
     4741 ATACCTGCCT CCCTGCACCC AGCCCCCGCG GCCTGAGCCT GCTGTGTCCC TCGTCCTCGG
     4801 CACCCCCAAT TCTCCCCCAG TGGCGAGGGA AGAGCCTAGA GTCTGCCTTC TGCTGAGCTG
     4861 TGTGTCAGGT GGATTTCCAG CCTGCACCCT CCCTCTGGGC AGAGCTAGGT TTATAGGCAC
     4921 CCAAGGGCTA CGGCTGCTCA AGCTACCAGA AGGGGCCTCG CCCTAGGGGG CCAGCCCCCA
     4981 GGGTCTTCTC CTGACCTTAT TCCTGTCAGG CAGCTACTGT GTGCAGAGCA TCTATAGGGA
     5041 ACTCAGGGAC ATCCGCTCTC CCTGCTTGCT TCCGTTAGGG GCCAGCTCAT CTTATAGGGA
     5101 CCTCCCACAT GTGAAGATCT GTGTCAGGCA GGAGCCAGAG GCCCGCACCT TCAAAAAAAC
     5161 CTTTGAGGTG GAGTAGACAG GGTGAGCTTT ACAGAGGCGC CAAGCCCCAC ATGCTATCGA
     5221 GTAGGCCTCA GTCAAGCATA GGGGCGAGGC CAAGAGAGGA CTGGAAAATG GGGTGGGGGA
     5281 CCCCCACCCT CTCCCTGGTG TGCAGAGGGG ACTCTGGAGG GCTGTTCACC TGTGGGTGAC
     5341 CCTGGCGCAG TTCCTAGAAC AGGCGGACAC ACAGATGAGC CCTACACTCT GGGTTATCCA
     5401 TGCAGCGCCT CTGCTGGCTT CTCCCTGCCC CTCCCCAGCA CCCTCTGGGG TCAGGCCCGA
     5461 AGTGAACCAG TGGGGAGCTG GTCCTGCTGT CCTGCTTAGA CCTCCAGATC TTGTCAGTGT
     5521 GGTTTTCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTTTC CACCCAATGT
     5581 CGAGCAGTGT GGTTTTGCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTTT
     5641 CCACCCAATG TCGAGCAAAC CCCGCCCAGC GTCTTGTCAT TGGCGAATTC GAACACGCAG
     5701 ATGCAGTCGG GGCGGCGCGG TCCCAGGTCC ACTTCGCATA TTAAGGTGAC GCGTGTGGCC
     5761 TCGAACACCG AGCGACCCTG CAGGTCCTCG CCATGGATCC TGATGATGTT GTTGATTCTT
     5821 CTAAATCTTT TGTGATGGAA AACTTTTCTT CGTACCACGG GACTAAACCT GGTTATGTAG
     5881 ATTCCATTCA AAAAGGTATA CAAAAGCCAA AATCTGGTAC ACAAGGAAAT TATGACGATG
     5941 ATTGGAAAGG GTTTTATAGT ACCGACAATA AATACGACGC TGCGGGATAC TCTGTAGATA
     6001 ATGAAAACCC GCTCTCTGGA AAAGCTGGAG GCGTGGTCAA AGTGACGTAT CCAGGACTGA
     6061 CGAAGGTTCT CGCACTAAAA GTGGATAATG CCGAAACTAT TAAGAAAGAG TTAGGTTTAA
     6121 GTCTCACTGA ACCGTTGATG GAGCAAGTCG GAACGGAAGA GTTTATCAAA AGGTTCGGTG
     6181 ATGGTGCTTC GCGTGTAGTG CTCAGCCTTC CCTTCGCTGA GGGGAGTTCT AGCGTTGAAT
     6241 ATATTAATAA CTGGGAACAG GCGAAAGCGT TAAGCGTAGA ACTTGAGATT AATTTTGAAA
     6301 CCCGTGGAAA ACGTGGCCAA GATGCGATGT ATGAGTATAT GGCTCAAGCC TGTGCAGGAA
     6361 ATCGTGTCAG GCGATCTCTT TGTGAAGGAA CCTTACTTCT GTGGTGTGAC ATAATTGGAC
     6421 AAACTACCTA CAGAGATTTA AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT
     6481 GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
     6541 TGGGAGCAGT GGTGGAATGC AGATCCACTA GAGTCGAGGG ATGCAGAAAT TGATGATCTA
     6601 TTAAACAATA AAGATGTCCA CTAAAATGGA AGTTTTTCCT GTCATACTTT GTTAAGAAGG
     6661 GTGAGAACAG AGTACCTACA TTTTGAATGG AAGGATTGGA GCTACGGGGG TGGGGGTGGG
     6721 GTGGGATTAG ATAAATGCCT GCTCTTTACT GAAGGCTCTT TACTATTGCT TTATGATAAT
     6781 GTTTCATAGT TGgatgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6841 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6901 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6961 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7021 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7081 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7141 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7201 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7261 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7321 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7381 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7441 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7501 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7561 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7621 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7681 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7741 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7801 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7861 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7921 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7981 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8041 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8101 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8161 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8221 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8281 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8341 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8401 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8461 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8521 gcg
//