LOCUS       CK542-_PB-cT3G-MG-Barco 7780 bp ds-DNA     circular     02-NOV-2022
DEFINITION  .
COMMENT     an expression clone created from CK530- PB-cT3G-MG-Barcoded-cERP2
            and CK261- pDONOR221 - SOX17an expression clone created from CK530-
            PB-cT3G-MG-Barcoded-cERP2  and CK261- pDONOR221 - SOX17
FEATURES             Location/Qualifiers
     extracted re... 1..375
                     /label="Extracted region from CK530- PB-cT3G-MG-Barcoded-cERP2 "
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Extracted interval: 1 -> 375 Original Circular Sequence Length: 6831"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="created by: wyssuser"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(1) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(2) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(3) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(4) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(5) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(6) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created by: wyssuser"
     protein_bind    368..375
                     /label="attR1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of&nbsp;<i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     attB1           369..394
                     /label="attB1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="annotation group: attB"
     extracted re... 376..1642
                     /label="Extracted region from CK261- pDONOR221 - SOX17"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Extracted interval: 652 -> 1918 Original Circular Sequence Length: 3813"
     regulatory      395..400
                     /label="Kozak"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="modified by: wyssuser"
     CDS             401..1661
                     /label="SOX17"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created by: wyssuser"
     extracted re... 1643..1661
                     /label="Extracted region from 15-sCK146-ORF-BC-AMP_Rev.ab1 (reversed)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Extracted interval: 671 -> 689 Original Circular Sequence Length: 3813"
     extracted re... 1662..1671
                     /label="Extracted region from CK261- pDONOR221 - SOX17"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 1938 -> 1947 Original Circular Sequence Length: 3813"
     attB2           complement(1662..1685)
                     /label="attB2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="annotation group: attB"
     protein_bind    complement(1672..1686)
                     /label="attR2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of <i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     extracted re... 1672..1749
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Extracted interval: 723 -> 800 Original Circular Sequence Length: 6786"
     CDS             1688..1714
                     /label="HA Tag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created by: wyssuser"
     terminator      1715..1723
                     /label="STOPS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="organism: synthetic DNA construct mol_type: other DNA locus_tag: source:synthetic DNA construct(4) ApEinfo_label: source:synthetic DNA construct ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: source modified by: wyssuser"
     primer_bind     1727..1749
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3"
     primer_bind     1727..1749
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     extracted re... 1750..1819
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Extracted interval: 8 -> 77 Original Circular Sequence Length: 6831"
     misc_difference 1754..1776
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="created by: wyssuser"
     misc_feature    1777..1796
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="created by: wyssuser modified by: wyssuser"
     misc_difference complement(1797..1819)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 1820..1823
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Extracted interval: 223 -> 226 Original Circular Sequence Length: 6769"
     primer_bind     complement(1824..1846)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: GAGTTCAGACGTGTGCTCTTCCGATCTNNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     primer_bind     complement(1824..1846)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3"
     extracted re... 1824..1855
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Extracted interval: -8 <- 23 Original Circular Sequence Length: 6769"
     motif           1847..1871
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 1856..1889
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Extracted interval: 259 -> 292 Original Circular Sequence Length: 6769"
     polyA_signal    1881..2015
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     extracted re... 1890..2045
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Extracted interval: 636 -> 791 Original Circular Sequence Length: 6529"
     extracted re... 2046..4756
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Extracted interval: 1035 -> 3745 Original Circular Sequence Length: 6769"
     repeat_region   complement(2287..2321)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac left (5') inverted repeat ApEinfo_label: piggyBac left (5') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region modified by: wyssuser"
     primer_bind     complement(2333..2348)
                     /label="M13F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: GTAAAACGACGGCCAG Hairpin Tm: None Tm: 52.4 Self Dimer Tm: 2.2 %GC: 56.3 created by: primer3"
     primer_bind     complement(2557..2576)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: pRS vectors, use to sequence yeast selectable marker locus_tag: pRS-marker ApEinfo_label: pRS-marker ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     2678..2700
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: pGEX vectors, reverse primer locus_tag: pGEX 3' ApEinfo_label: pGEX 3' ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     complement(2739..2757)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: pBR322 vectors, upsteam of EcoRI site, forward primer locus_tag: pBRforEco ApEinfo_label: pBRforEco ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     promoter        2825..2929
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="gene: bla locus_tag: AmpR promoter ApEinfo_label: AmpR promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     CDS             2930..3790
                     /label="AmpR"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="codon_start: 1 gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, and related antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW locus_tag: AmpR ApEinfo_label: AmpR ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     primer_bind     complement(3148..3167)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: Ampicillin resistance gene, reverse primer locus_tag: Amp-R ApEinfo_label: Amp-R ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     rep_origin      3961..4549
                     /label="ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication locus_tag: ori ApEinfo_label: ori ApEinfo_fwdcolor: #999999 ApEinfo_revcolor: #999999 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: rep_origin"
     primer_bind     4450..4469
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: pBR322 origin, forward primer locus_tag: pBR322ori-F ApEinfo_label: pBR322ori-F ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     4703..4720
                     /label="L4440"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: L4440 vector, forward primer locus_tag: L4440 ApEinfo_label: L4440 ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     extracted re... 4757..4845
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Extracted interval: 278 -> 366 Original Circular Sequence Length: 6769"
     extracted re... 4846..7714
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Extracted interval: 3835 -> 6703 Original Circular Sequence Length: 6769"
     protein_bind    4926..4947
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presenceof cAMP. locus_tag: CAP binding site ApEinfo_label: CAP binding site ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
     promoter        4962..4992
                     /label="lac promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: promoter for the E. coli lac operon locus_tag: lac promoter ApEinfo_label: lac promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     protein_bind    5000..5016
                     /label="lac operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). locus_tag: lac operator ApEinfo_label: lac operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
     primer_bind     5024..5040
                     /label="M13R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: common sequencing primer, one of multiple similar variants locus_tag: M13 rev ApEinfo_label: M13 rev ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind Sequence: CAGGAAACAGCTATGAC Hairpin Tm: None Tm: 49.1 Self Dimer Tm: None %GC: 47.1 created by: primer3 modified by: wyssuser"
     repeat_region   5066..5128
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac right (3') inverted repeat ApEinfo_label: piggyBac right (3') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region"
     polyA_signal    5304..5385
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal(1) ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     primer_bind     5412..5431
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTTTCAGGTTCAGGGGGAGG Hairpin Tm: None Tm: 60.0 Self Dimer Tm: None %GC: 60.0 modified by: wyssuser"
     CDS             complement(5502..6101)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="created by: wyssuser"
     primer_bind     5987..6006
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: GATCTACGGTATGGCGGGTC Hairpin Tm: None Tm: 59.8 Self Dimer Tm: None %GC: 60.0 Pair Dimer Tm: None Product Size: 35 annotation group: 69733439644894ee5c5e1-3592-4568-a0da-b1c47594b8cdFinal Plasmid modified by: wyssuser"
     CDS             complement(6102..6158)
                     /label="P2A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 product: 2A peptide from porcine teschovirus-1 polyprotein note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. translation: ATNFSLLKQAGDVEENPGP locus_tag: P2A ApEinfo_label: P2A ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     CDS             complement(6168..6911)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="created by: wyssuser"
     primer_bind     complement(6309..6328)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTGGCCTGGAGAAACAGCTA Hairpin Tm: 37.2 Tm: 59.7 Self Dimer Tm: 3.3 %GC: 55.0 modified by: wyssuser"
     primer_bind     6554..6573
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GGAACGCGAGCTGATTTTCC Hairpin Tm: None Tm: 59.9 Self Dimer Tm: 15.9 %GC: 55.0 modified by: wyssuser"
     CDS             complement(6912..6935)
                     /label="FLAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="codon_start: 1 product: FLAG(R) epitope tag, followed by an enterokinasecleavage site translation: DYKDDDDK locus_tag: FLAG ApEinfo_label: FLAG ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     regulatory      complement(6935..6944)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="regulatory_class: other note: vertebrate consensus sequence for strong initiationof translation (Kozak, 1987) locus_tag: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_label: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: regulatory modified by: wyssuser"
     promoter        complement(6964..7175)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: core promoter for human elongation factor EF-1-alpha locus_tag: EF-1-alpha core promoter ApEinfo_label: EF-1-alpha core promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     regulatory      complement(7176..7555)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created by: wyssuser modified by: wyssuser"
     primer_bind     7704..7723
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: TTTGAGTAGCGACTGACGGG annotation group: 646536956143517519a63-5614-47f8-a332-a32195d8b3a1Final Plasmid modified by: wyssuser Hairpin Tm: 42.8 Tm: 59.8 Self Dimer Tm: 3.5 %GC: 55.0 Pair Dimer Tm: None Product Size: 614"
     extracted re... 7715..7747
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Extracted interval: 5833 -> 5865 Original Circular Sequence Length: 5865"
     extracted re... 7748..7780
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Extracted interval: 6503 -> 6535 Original Circular Sequence Length: 6535"
     primer_bind     complement(7757..7775)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: CACTCGAGTGGCTCCGGTG Hairpin Tm: 42.1 Tm: 62.6 Self Dimer Tm: 33.4 %GC: 68.4 modified by: wyssuser"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTTAGCCACC ATGAGCAGCC CGGATGCGGG
      421 ATACGCCAGT GACGACCAGA GCCAGACCCA GAGCGCGCTG CCCGCGGTGA TGGCCGGGCT
      481 GGGCCCCTGC CCCTGGGCCG AGTCGCTGAG CCCCATCGGG GACATGAAGG TGAAGGGCGA
      541 GGCGCCGGCG AACAGCGGAG CACCGGCCGG GGCCGCGGGC CGAGCCAAGG GCGAGTCCCG
      601 TATCCGGCGG CCGATGAACG CTTTCATGGT GTGGGCTAAG GACGAGCGCA AGCGGCTGGC
      661 GCAGCAGAAT CCAGACCTGC ACAACGCCGA GTTGAGCAAG ATGCTGGGCA AGTCGTGGAA
      721 GGCGCTGACG CTGGCGGAGA AGCGGCCCTT CGTGGAGGAG GCAGAGCGGC TGCGCGTGCA
      781 GCACATGCAG GACCACCCCA ACTACAAGTA CCGGCCGCGG CGGCGCAAGC AGGTGAAGCG
      841 GCTGAAGCGG GTGGAGGGCG GCTTCCTGCA CGGCCTGGCT GAGCCGCAGG CGGCCGCGCT
      901 GGGCCCCGAG GGCGGCCGCG TGGCCATGGA CGGCCTGGGC CTCCAGTTCC CCGAGCAGGG
      961 CTTCCCCGCC GGCCCGCCGC TGCTGCCTCC GCACATGGGC GGCCACTACC GCGACTGCCA
     1021 GAGTCTGGGC GCGCCTCCGC TCGACGGCTA CCCGTTGCCC ACGCCCGACA CGTCCCCGCT
     1081 GGACGGCGTG GACCCCGACC CGGCTTTCTT CGCCGCCCCG ATGCCCGGGG ACTGCCCGGC
     1141 GGCCGGCACC TACAGCTACG CGCAGGTCTC GGACTACGCT GGCCCCCCGG AGCCTCCCGC
     1201 CGGTCCCATG CACCCCCGAC TCGGCCCAGA GCCCGCGGGT CCCTCGATTC CGGGCCTCCT
     1261 GGCGCCACCC AGCGCCCTTC ACGTGTACTA CGGCGCGATG GGCTCGCCCG GGGCGGGCGG
     1321 CGGGCGCGGC TTCCAGATGC AGCCGCAACA CCAGCACCAG CACCAGCACC AGCACCACCC
     1381 CCCGGGCCCC GGACAGCCGT CGCCCCCTCC GGAGGCACTG CCCTGCCGGG ACGGCACGGA
     1441 CCCCAGTCAG CCCGCCGAGC TCCTCGGGGA GGTGGACCGC ACGGAATTTG AACAGTATCT
     1501 GCACTTCGTG TGCAAGCCTG AGATGGGCCT CCCCTACCAG GGGCATGACT CCGGTGTGAA
     1561 TCTCCCCGAC AGCCACGGGG CCATTTCCTC GGTGGTGTCC GACGCCAGCT CCGCGGTATA
     1621 TTACTGCAAC TATCCTGACG TGTAGCTCGA GTGCGGCCGC AACCCAGCTT TCTTGTACAA
     1681 AGTGGTATAC CCATACGATG TTCCAGATTA CGCTTAGTAA TGAGAAATAC TCAGAAGATG
     1741 TCACTCACCA TAGTCCGCAG TGAAAGATAA ATGATCNNNN NNNNNNNNNN NNNNNNGTTT
     1801 TAGAGCTAGA AATAGCAAGT AGGCCACTGA GCAAGAACTA TGAATGAGAT CTTTAGACAT
     1861 ATTGCGGCCG CTTTCTCCGC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
     1921 CCAAAACTAG AATGCAGTGA AAAAAATGCC TTATTTGTGA AATTTGTGAT GCTATTGCCT
     1981 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTATCTA TAACAAGAAA ATATATATAT
     2041 AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC
     2101 TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA
     2161 AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG
     2221 AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA
     2281 AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAAGAATT CACTGGCCGT
     2341 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
     2401 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA
     2461 ACAGTTGCGC AGCCTGAATG GCGAATGGCG CCTGATGCGG TATTTTCTCC TTACGCATCT
     2521 GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     2581 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     2641 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CATGTCTCCG GGAGCTGCAT GTGTCAGAGG
     2701 TTTTCACCGT CATCACCGAA ACACGCGAGT TCGAAAGGGC CTCGTGATAC GCCTATTTTT
     2761 ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA
     2821 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     2881 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
     2941 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
     3001 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
     3061 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
     3121 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
     3181 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
     3241 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
     3301 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
     3361 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
     3421 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
     3481 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
     3541 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
     3601 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGTTCAC GCGGTATCAT
     3661 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
     3721 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
     3781 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     3841 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     3901 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     3961 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     4021 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     4081 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     4141 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     4201 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     4261 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     4321 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     4381 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     4441 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     4501 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     4561 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
     4621 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
     4681 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGATG AGCGCCCAAT
     4741 ACGCAAACCG CCTCTCACCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     4801 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
     4861 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
     4921 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
     4981 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
     5041 CATGATTACG CCAAGGTCGA CTTAACCCTA GAAAGATAAT CATATTGTGA CGTACGTTAA
     5101 AGATAATCAT GCGTAAAATT GACGCATGTG TTTTATCGGT CTGTATATCG AGGTTTATTT
     5161 ATTAATTTGA ATAGATATTA AGTTTTATTA TATTTACACT TACATACTAA TAATAAATTC
     5221 AACAAACAAT TTATTTATGT TTATTTATTT ATTAAAAAAA AACAAAAACT CAAAATTTCT
     5281 TCTATAAAGT AACAAAACTT TTAGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT
     5341 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC
     5401 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC
     5461 CTCTACAAAT GTGGTATGGC TGATTATGAT CCTCTGGAGA TTCATGCGCC AGGTTTTCTG
     5521 GTCATGCACC ACGTTCGTGG GCCCTCAGGA ACCTCCACGT CAGCGGTAAC TGTGAAGCCC
     5581 AGTCGCTCGT AAAAAGGAAG ATTCCGGGGA GCACTGGTTT CGAGGAACGC CGGCACGCCA
     5641 GCCCGTTCCG CGGCCTCAAC ACCAGGAAGA ACGACAGCGC TTCCCAAACC TTTACCTTGG
     5701 TGATCTGGGC TGACACCGAC AGTGGCAAGA AACCACGCAG GCTCCTTTGG TCTATGAGGA
     5761 GCCAGCAAGC CCTCCATTTG CTGTTGTGCC GCCAACCTAC TCCCTGACAG CTCGGCCATC
     5821 CGCGGACCTA TTTCCGCGAA CACTGCGCCG GCTTCGACGC TTTCCGGAGT AGTCCAGACC
     5881 GCAACTGCGG CACCATCGTC CGCAACCCAA ACTTTACCAA TGTCCAGACC CACGCGCGTA
     5941 AGAAACAGCT CTTGCAACTC AGTTACCCTC TCTATGTGTC TGTCCGGATC TACGGTATGG
     6001 CGGGTCGCTG GATAATCTGC AAATGCTGCG GCAAGTGTTC GGACAGCCCG TGGTACATCG
     6061 TCACGGGTAG CGAGCCTAAC AGTGGGCTTA TATTCCGTCA TGGGGCCGGG GTTCTCCTCC
     6121 ACGTCGCCGG CCTGCTTCAG CAGGCTGAAG TTGGTGGCGC CGCTGCCCCC GGGGAGCATG
     6181 TCAAGGTCAA AATCGTCAAG AGCGTCAGCA GGCAGCATAT CAAGGTCAAA GTCGTCAAGG
     6241 GCATCGGCTG GGAGCATGTC TAAGTCAAAA TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT
     6301 TCGCACTTTA GCTGTTTCTC CAGGCCACAT ATGATTAGTT CCAGGCCGAA AAGGAAGGCA
     6361 GGTTCGGCTC CCTGCCGGTC GAACAGCTCA ATTGCTTGTT TCAGAAGTGG GGGCATAGAA
     6421 TCGGTGGTAG GTGTCTCTCT TTCCTCTTTT GCTACTTGAT GCTCCTGTTC CTCCAATACG
     6481 CAGCCCAGTG TAAAGTGGCC CACGGCGGAC AGAGCGTACA GTGCGTTCTC CAGGGAGAAG
     6541 CCTTGCTGAC ACAGGAACGC GAGCTGATTT TCCAGGGTTT CGTACTGTTT CTCTGTTGGG
     6601 CGGGTGCCGA GATGCACTTT AGCCCCGTCA CGATGTGAGA GGAGAGCACA GCGGTATGAC
     6661 TTGGCGTTGT TCCGCAGAAA GTCTTGCCAT GACTCGCCTT CCAGGGGGCA GGAGTGGGTA
     6721 TGATGCCTGT CCAGCATCTC GATTGGCAGG GCATCGAGCA GGGCCCGCTT GTTCTTCACG
     6781 TGCCAGTACA GGGTAGGCTG CTCAACTCCC AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG
     6841 CCTTCGATAC CGACTCCATT GAGTAATTCC AGAGCAGAGT TTATGACTTT GCTCTTGTCC
     6901 AGTCTAGACA TCTTATCGTC ATCGTCTTTG TAATCCATGG TGGCGGATCC CGCGTCACGA
     6961 CACCTGTGTT CTGGCGGCAA ACCCGTTGCG AAAAAGAACG TTCACGGCGA CTACTGCACT
     7021 TATATACGGT TCTCCCCCAC CCTCGGGAAA AAGGCGGAGC CAGTACACGA CATCACTTTC
     7081 CCAGTTTACC CCGCGCCACC TTCTCTAGGC ACCGGTTCAA TTGCCGACCC CTCCCCCCAA
     7141 CTTCTCGGGC ACTGTGGGCC ATGTGCGCTC TGCCCCATGG TAATAGCCAT GACTAATACG
     7201 TAGATGTACT GCCAAGTAGG AAAGTGCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG
     7261 GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT
     7321 ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTGCCT
     7381 ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC
     7441 GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA
     7501 TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAG TCAATAATCA ATGTCATAAG
     7561 CTCTAATCGC ATAATCGAGC TAAGAGCGAT TAATATGCTT AGCTAGCTTA GCTAAGCTAG
     7621 ATTTCTCTAT TCTCCCACAA TATATAGCAG CTATTAGCTT AGCAGTCCGA ATACGATCGT
     7681 CCACGATCCA TAAGCGTAAC ACATTTGAGT AGCGACTGAC GGGCACCGGA GCCACTCGAG
     7741 TGGAATTACT GACGGGCACC GGAGCCACTC GAGTGGAATT
//