LOCUS       CK570-_PB-cT3G-MG-Barco 7333 bp ds-DNA     circular     02-NOV-2022
DEFINITION  .
COMMENT     an expression clone created from CK530- PB-cT3G-MG-Barcoded-cERP2
            and CK306- pDONOR221 - HHEXan expression clone created from CK530-
            PB-cT3G-MG-Barcoded-cERP2  and CK306- pDONOR221 - HHEX
FEATURES             Location/Qualifiers
     extracted re... 1..375
                     /label="Extracted region from CK530- PB-cT3G-MG-Barcoded-cERP2 "
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Extracted interval: 1 -> 375 Original Circular Sequence Length: 6831"
     promoter        9..264
                     /label="TRE3GS Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created by: wyssuser"
     protein_bind    17..35
                     /label="tet operator (1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    53..71
                     /label="tet operator (2)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(1) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    89..107
                     /label="tet operator (3)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(2) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    125..143
                     /label="tet operator (4)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(3) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    161..179
                     /label="tet operator (5)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(4) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    197..215
                     /label="tet operator (6)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(5) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     protein_bind    233..251
                     /label="tet operator (7)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="gene: tetO bound_moiety: tetracycline repressor TetR note: bacterial operator O2 for the tetR and tetA genes locus_tag: tet operator(6) ApEinfo_label: tet operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind modified by: wyssuser"
     promoter        265..364
                     /label="mCMV Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created by: wyssuser"
     protein_bind    368..375
                     /label="attR1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of&nbsp;<i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     attB1           369..394
                     /label="attB1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="annotation group: attB"
     extracted re... 376..761
                     /label="Extracted region from HHEX - 3087"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Extracted interval: 8 -> 393 Original Circular Sequence Length: 3366"
     misc_feature    395..400
                     /label="Kozak"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Color: -1"
     mRNA            401..761
                     /label="HHEX mRNA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX product: hematopoietically expressed homeobox note: Derived by automated computational analysis using gene prediction method: BestRefSeq. transcript_id: NM_002729.5 db_xref: Ensembl:ENST00000282728.10 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 transcription: AGCTCTGCGAGGGGCCGGAGCGCGGCGGAGCCATGCAGTACCCGCACCCCGGGCCGGCGGCGGGCGCCGTGGGGGTGCCGCTGTACGCGCCCACGCCGCTGCTGCAACCCGCACACCCGACGCCCTTTTACATCGAGGACATCCTGGGCCGCGGGCCCGCCGCGCCCACGCCCGCCCCCACGCTGCCGTCCCCCAACTCCTCCTTCACCAGCCTCGTGTCCCCCTACCGGACCCCGGTGTACGAGCCCACGCCGATCCATCCAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTACGGACCCGGCGGCTTCGGGGGCCCTCTGTACCCCTTCCCGCGGACGGTGAACGACTACACGCACGCCCTGCTCCGCCACGACCCCCTGGGCAAACCTCTACTCTGGAGCCCCTTCTTGCAGAGGCCTCTGCATAAAAGGAAAGGCGGCCAGGTGAGATTCTCCAACGACCAGACCATCGAGCTGGAGAAGAAATTCGAGACGCAGAAATATCTCTCTCCGCCCGAGAGGAAGCGTCTGGCCAAGATGCTGCAGCTCAGCGAGAGACAGGTCAAAACCTGGTTTCAGAATCGACGCGCTAAATGGAGGAGACTAAAACAGGAGAACCCTCAAAGCAATAAAAAAGAAGAACTGGAAAGTTTGGACAGTTCCTGTGATCAGAGGCAAGATTTGCCCAGTGAACAGAATAAAGGTGCTTCTTTGGATAGCTCTCAATGTTCGCCCTCCCCTGCCTCCCAGGAAGACCTTGAATCAGAGATTTCAGAGGATTCTGATCAGGAAGTGGACATTGAGGGCGATAAAAGCTATTTTAATGCTGGATGATGACCACTGGCATTGGCATGTTCAGAAAACTGGATTTAGGAATAATGTTTTGCTACAGAAAATCTTCATAGAAGAACTGGAAGGCTATATAAGAAAGGGAATCAATTCTCTGGTATTCTGGAAACCTAAAAATATTTGGTGCACTGCTCAATTAACAAACCTACATGGAGACCTTAATTTTGACTTAACAAATAGTTTATGTACTGCTCTTAGGTTGTTTTGATAAAGTGACATTATAGTGATTAAATTCTTCCCCCTTTAAAAAAACAGTTAGTGGTTTTCACTATTTATAAAAAATTAATTTTGAACTTTTTGTTAAATTTTTAAGTTATAGCTTTAAAGGTTTTAATAGGACCTTCTTGAACGACTTTTCTGTAATCTGTTTATCTCCCACTTAATGGAAAGGCAAAGGGGTACCCCAAATCCAGAGGTGCCTACATTTCAGGCAGCCTTGGAGTATTTTAAAAGGAAAACATTCTTTACTTTTATATGACATTCTTATACTGCTGTCTCAAATCCAAAAACATTTCAGAGCTCTTGTCTCAGAGATGTGTGTTCTTTTTGTCAGAGATATGGTTGATGAGAATCTTAAATGCTTGTTTTGCACTATCACTTAGTACCTGTTTGACCAAGGTGTTAAGGGGATAGTACCTCCCAATTCAAGCAGAGAAACTGACCTGACTAAAGTTAATCGCAGATGAACTAGAAGTCACAGGTTAATTAAATGTAAGTAGATTGTAGATACTGTTTTATATCAAACAATGTTTATAATGTGTATATAGAATTGTTCACTGTAAAAAAAATGGCCAAAATGTGTTTTTTTTTTAATAAGTAACTTGACTATAAAATAAAGCCGTCCGTGGGACGACTGACCTCGTT NCBI Feature Key: mRNA NCBI Join Type: join"
     CDS             401..761
                     /label="HHEX CDS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX note: Derived by automated computational analysis using gene prediction method: BestRefSeq. codon_start: 1 transl_table: 1 product: hematopoietically-expressed homeobox protein HHEX protein_id: NP_002720.1 db_xref: GI:4506049 db_xref: CCDS:CCDS7423.1 db_xref: Ensembl:ENSP00000282728.5 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 translation: MQYPHPGPAAGAVGVPLYAPTPLLQPAHPTPFYIEDILGRGPAAPTPAPTLPSPNSSFTSLVSPYRTPVYEPTPIHPAFSHHSAAALAAAYGPGGFGGPLYPFPRTVNDYTHALLRHDPLGKPLLWSPFLQRPLHKRKGGQVRFSNDQTIELEKKFETQKYLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQENPQSNKKEELESLDSSCDQRQDLPSEQNKGASLDSSQCSPSPASQEDLESEISEDSDQEVDIEGDKSYFNAG NCBI Feature Key: CDS NCBI Join Type: join"
     mRNA            762..940
                     /label="HHEX mRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX product: hematopoietically expressed homeobox note: Derived by automated computational analysis using gene prediction method: BestRefSeq. transcript_id: NM_002729.5 db_xref: Ensembl:ENST00000282728.10 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 transcription: AGCTCTGCGAGGGGCCGGAGCGCGGCGGAGCCATGCAGTACCCGCACCCCGGGCCGGCGGCGGGCGCCGTGGGGGTGCCGCTGTACGCGCCCACGCCGCTGCTGCAACCCGCACACCCGACGCCCTTTTACATCGAGGACATCCTGGGCCGCGGGCCCGCCGCGCCCACGCCCGCCCCCACGCTGCCGTCCCCCAACTCCTCCTTCACCAGCCTCGTGTCCCCCTACCGGACCCCGGTGTACGAGCCCACGCCGATCCATCCAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTACGGACCCGGCGGCTTCGGGGGCCCTCTGTACCCCTTCCCGCGGACGGTGAACGACTACACGCACGCCCTGCTCCGCCACGACCCCCTGGGCAAACCTCTACTCTGGAGCCCCTTCTTGCAGAGGCCTCTGCATAAAAGGAAAGGCGGCCAGGTGAGATTCTCCAACGACCAGACCATCGAGCTGGAGAAGAAATTCGAGACGCAGAAATATCTCTCTCCGCCCGAGAGGAAGCGTCTGGCCAAGATGCTGCAGCTCAGCGAGAGACAGGTCAAAACCTGGTTTCAGAATCGACGCGCTAAATGGAGGAGACTAAAACAGGAGAACCCTCAAAGCAATAAAAAAGAAGAACTGGAAAGTTTGGACAGTTCCTGTGATCAGAGGCAAGATTTGCCCAGTGAACAGAATAAAGGTGCTTCTTTGGATAGCTCTCAATGTTCGCCCTCCCCTGCCTCCCAGGAAGACCTTGAATCAGAGATTTCAGAGGATTCTGATCAGGAAGTGGACATTGAGGGCGATAAAAGCTATTTTAATGCTGGATGATGACCACTGGCATTGGCATGTTCAGAAAACTGGATTTAGGAATAATGTTTTGCTACAGAAAATCTTCATAGAAGAACTGGAAGGCTATATAAGAAAGGGAATCAATTCTCTGGTATTCTGGAAACCTAAAAATATTTGGTGCACTGCTCAATTAACAAACCTACATGGAGACCTTAATTTTGACTTAACAAATAGTTTATGTACTGCTCTTAGGTTGTTTTGATAAAGTGACATTATAGTGATTAAATTCTTCCCCCTTTAAAAAAACAGTTAGTGGTTTTCACTATTTATAAAAAATTAATTTTGAACTTTTTGTTAAATTTTTAAGTTATAGCTTTAAAGGTTTTAATAGGACCTTCTTGAACGACTTTTCTGTAATCTGTTTATCTCCCACTTAATGGAAAGGCAAAGGGGTACCCCAAATCCAGAGGTGCCTACATTTCAGGCAGCCTTGGAGTATTTTAAAAGGAAAACATTCTTTACTTTTATATGACATTCTTATACTGCTGTCTCAAATCCAAAAACATTTCAGAGCTCTTGTCTCAGAGATGTGTGTTCTTTTTGTCAGAGATATGGTTGATGAGAATCTTAAATGCTTGTTTTGCACTATCACTTAGTACCTGTTTGACCAAGGTGTTAAGGGGATAGTACCTCCCAATTCAAGCAGAGAAACTGACCTGACTAAAGTTAATCGCAGATGAACTAGAAGTCACAGGTTAATTAAATGTAAGTAGATTGTAGATACTGTTTTATATCAAACAATGTTTATAATGTGTATATAGAATTGTTCACTGTAAAAAAAATGGCCAAAATGTGTTTTTTTTTTAATAAGTAACTTGACTATAAAATAAAGCCGTCCGTGGGACGACTGACCTCGTT NCBI Feature Key: mRNA NCBI Join Type: join"
     CDS             762..940
                     /label="HHEX CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX note: Derived by automated computational analysis using gene prediction method: BestRefSeq. codon_start: 1 transl_table: 1 product: hematopoietically-expressed homeobox protein HHEX protein_id: NP_002720.1 db_xref: GI:4506049 db_xref: CCDS:CCDS7423.1 db_xref: Ensembl:ENSP00000282728.5 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 translation: MQYPHPGPAAGAVGVPLYAPTPLLQPAHPTPFYIEDILGRGPAAPTPAPTLPSPNSSFTSLVSPYRTPVYEPTPIHPAFSHHSAAALAAAYGPGGFGGPLYPFPRTVNDYTHALLRHDPLGKPLLWSPFLQRPLHKRKGGQVRFSNDQTIELEKKFETQKYLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQENPQSNKKEELESLDSSCDQRQDLPSEQNKGASLDSSQCSPSPASQEDLESEISEDSDQEVDIEGDKSYFNAG NCBI Feature Key: CDS NCBI Join Type: join"
     extracted re... 762..940
                     /label="Extracted region from HHEX - 3087"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Extracted interval: 2414 -> 2592 Original Circular Sequence Length: 3366"
     CDS             941..991
                     /label="HHEX CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX note: Derived by automated computational analysis using gene prediction method: BestRefSeq. codon_start: 1 transl_table: 1 product: hematopoietically-expressed homeobox protein HHEX protein_id: NP_002720.1 db_xref: GI:4506049 db_xref: CCDS:CCDS7423.1 db_xref: Ensembl:ENSP00000282728.5 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 translation: MQYPHPGPAAGAVGVPLYAPTPLLQPAHPTPFYIEDILGRGPAAPTPAPTLPSPNSSFTSLVSPYRTPVYEPTPIHPAFSHHSAAALAAAYGPGGFGGPLYPFPRTVNDYTHALLRHDPLGKPLLWSPFLQRPLHKRKGGQVRFSNDQTIELEKKFETQKYLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQENPQSNKKEELESLDSSCDQRQDLPSEQNKGASLDSSQCSPSPASQEDLESEISEDSDQEVDIEGDKSYFNAG NCBI Feature Key: CDS NCBI Join Type: join"
     extracted re... 941..991
                     /label="Extracted region from HHEX - 3087"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="Extracted interval: 2748 -> 2798 Original Circular Sequence Length: 3366"
     mRNA            941..991
                     /label="HHEX mRNA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX product: hematopoietically expressed homeobox note: Derived by automated computational analysis using gene prediction method: BestRefSeq. transcript_id: NM_002729.5 db_xref: Ensembl:ENST00000282728.10 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 transcription: AGCTCTGCGAGGGGCCGGAGCGCGGCGGAGCCATGCAGTACCCGCACCCCGGGCCGGCGGCGGGCGCCGTGGGGGTGCCGCTGTACGCGCCCACGCCGCTGCTGCAACCCGCACACCCGACGCCCTTTTACATCGAGGACATCCTGGGCCGCGGGCCCGCCGCGCCCACGCCCGCCCCCACGCTGCCGTCCCCCAACTCCTCCTTCACCAGCCTCGTGTCCCCCTACCGGACCCCGGTGTACGAGCCCACGCCGATCCATCCAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTACGGACCCGGCGGCTTCGGGGGCCCTCTGTACCCCTTCCCGCGGACGGTGAACGACTACACGCACGCCCTGCTCCGCCACGACCCCCTGGGCAAACCTCTACTCTGGAGCCCCTTCTTGCAGAGGCCTCTGCATAAAAGGAAAGGCGGCCAGGTGAGATTCTCCAACGACCAGACCATCGAGCTGGAGAAGAAATTCGAGACGCAGAAATATCTCTCTCCGCCCGAGAGGAAGCGTCTGGCCAAGATGCTGCAGCTCAGCGAGAGACAGGTCAAAACCTGGTTTCAGAATCGACGCGCTAAATGGAGGAGACTAAAACAGGAGAACCCTCAAAGCAATAAAAAAGAAGAACTGGAAAGTTTGGACAGTTCCTGTGATCAGAGGCAAGATTTGCCCAGTGAACAGAATAAAGGTGCTTCTTTGGATAGCTCTCAATGTTCGCCCTCCCCTGCCTCCCAGGAAGACCTTGAATCAGAGATTTCAGAGGATTCTGATCAGGAAGTGGACATTGAGGGCGATAAAAGCTATTTTAATGCTGGATGATGACCACTGGCATTGGCATGTTCAGAAAACTGGATTTAGGAATAATGTTTTGCTACAGAAAATCTTCATAGAAGAACTGGAAGGCTATATAAGAAAGGGAATCAATTCTCTGGTATTCTGGAAACCTAAAAATATTTGGTGCACTGCTCAATTAACAAACCTACATGGAGACCTTAATTTTGACTTAACAAATAGTTTATGTACTGCTCTTAGGTTGTTTTGATAAAGTGACATTATAGTGATTAAATTCTTCCCCCTTTAAAAAAACAGTTAGTGGTTTTCACTATTTATAAAAAATTAATTTTGAACTTTTTGTTAAATTTTTAAGTTATAGCTTTAAAGGTTTTAATAGGACCTTCTTGAACGACTTTTCTGTAATCTGTTTATCTCCCACTTAATGGAAAGGCAAAGGGGTACCCCAAATCCAGAGGTGCCTACATTTCAGGCAGCCTTGGAGTATTTTAAAAGGAAAACATTCTTTACTTTTATATGACATTCTTATACTGCTGTCTCAAATCCAAAAACATTTCAGAGCTCTTGTCTCAGAGATGTGTGTTCTTTTTGTCAGAGATATGGTTGATGAGAATCTTAAATGCTTGTTTTGCACTATCACTTAGTACCTGTTTGACCAAGGTGTTAAGGGGATAGTACCTCCCAATTCAAGCAGAGAAACTGACCTGACTAAAGTTAATCGCAGATGAACTAGAAGTCACAGGTTAATTAAATGTAAGTAGATTGTAGATACTGTTTTATATCAAACAATGTTTATAATGTGTATATAGAATTGTTCACTGTAAAAAAAATGGCCAAAATGTGTTTTTTTTTTAATAAGTAACTTGACTATAAAATAAAGCCGTCCGTGGGACGACTGACCTCGTT NCBI Feature Key: mRNA NCBI Join Type: join"
     CDS             992..1213
                     /label="HHEX CDS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX note: Derived by automated computational analysis using gene prediction method: BestRefSeq. codon_start: 1 transl_table: 1 product: hematopoietically-expressed homeobox protein HHEX protein_id: NP_002720.1 db_xref: GI:4506049 db_xref: CCDS:CCDS7423.1 db_xref: Ensembl:ENSP00000282728.5 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 translation: MQYPHPGPAAGAVGVPLYAPTPLLQPAHPTPFYIEDILGRGPAAPTPAPTLPSPNSSFTSLVSPYRTPVYEPTPIHPAFSHHSAAALAAAYGPGGFGGPLYPFPRTVNDYTHALLRHDPLGKPLLWSPFLQRPLHKRKGGQVRFSNDQTIELEKKFETQKYLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQENPQSNKKEELESLDSSCDQRQDLPSEQNKGASLDSSQCSPSPASQEDLESEISEDSDQEVDIEGDKSYFNAG NCBI Feature Key: CDS NCBI Join Type: join"
     mRNA            992..1213
                     /label="HHEX mRNA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="gene: HHEX gene_synonym: HEX; HMPH; HOX11L-PEN; PRH; PRHX product: hematopoietically expressed homeobox note: Derived by automated computational analysis using gene prediction method: BestRefSeq. transcript_id: NM_002729.5 db_xref: Ensembl:ENST00000282728.10 db_xref: GeneID:3087 db_xref: HGNC:HGNC:4901 db_xref: MIM:604420 transcription: AGCTCTGCGAGGGGCCGGAGCGCGGCGGAGCCATGCAGTACCCGCACCCCGGGCCGGCGGCGGGCGCCGTGGGGGTGCCGCTGTACGCGCCCACGCCGCTGCTGCAACCCGCACACCCGACGCCCTTTTACATCGAGGACATCCTGGGCCGCGGGCCCGCCGCGCCCACGCCCGCCCCCACGCTGCCGTCCCCCAACTCCTCCTTCACCAGCCTCGTGTCCCCCTACCGGACCCCGGTGTACGAGCCCACGCCGATCCATCCAGCCTTCTCGCACCACTCCGCCGCCGCGCTGGCCGCTGCCTACGGACCCGGCGGCTTCGGGGGCCCTCTGTACCCCTTCCCGCGGACGGTGAACGACTACACGCACGCCCTGCTCCGCCACGACCCCCTGGGCAAACCTCTACTCTGGAGCCCCTTCTTGCAGAGGCCTCTGCATAAAAGGAAAGGCGGCCAGGTGAGATTCTCCAACGACCAGACCATCGAGCTGGAGAAGAAATTCGAGACGCAGAAATATCTCTCTCCGCCCGAGAGGAAGCGTCTGGCCAAGATGCTGCAGCTCAGCGAGAGACAGGTCAAAACCTGGTTTCAGAATCGACGCGCTAAATGGAGGAGACTAAAACAGGAGAACCCTCAAAGCAATAAAAAAGAAGAACTGGAAAGTTTGGACAGTTCCTGTGATCAGAGGCAAGATTTGCCCAGTGAACAGAATAAAGGTGCTTCTTTGGATAGCTCTCAATGTTCGCCCTCCCCTGCCTCCCAGGAAGACCTTGAATCAGAGATTTCAGAGGATTCTGATCAGGAAGTGGACATTGAGGGCGATAAAAGCTATTTTAATGCTGGATGATGACCACTGGCATTGGCATGTTCAGAAAACTGGATTTAGGAATAATGTTTTGCTACAGAAAATCTTCATAGAAGAACTGGAAGGCTATATAAGAAAGGGAATCAATTCTCTGGTATTCTGGAAACCTAAAAATATTTGGTGCACTGCTCAATTAACAAACCTACATGGAGACCTTAATTTTGACTTAACAAATAGTTTATGTACTGCTCTTAGGTTGTTTTGATAAAGTGACATTATAGTGATTAAATTCTTCCCCCTTTAAAAAAACAGTTAGTGGTTTTCACTATTTATAAAAAATTAATTTTGAACTTTTTGTTAAATTTTTAAGTTATAGCTTTAAAGGTTTTAATAGGACCTTCTTGAACGACTTTTCTGTAATCTGTTTATCTCCCACTTAATGGAAAGGCAAAGGGGTACCCCAAATCCAGAGGTGCCTACATTTCAGGCAGCCTTGGAGTATTTTAAAAGGAAAACATTCTTTACTTTTATATGACATTCTTATACTGCTGTCTCAAATCCAAAAACATTTCAGAGCTCTTGTCTCAGAGATGTGTGTTCTTTTTGTCAGAGATATGGTTGATGAGAATCTTAAATGCTTGTTTTGCACTATCACTTAGTACCTGTTTGACCAAGGTGTTAAGGGGATAGTACCTCCCAATTCAAGCAGAGAAACTGACCTGACTAAAGTTAATCGCAGATGAACTAGAAGTCACAGGTTAATTAAATGTAAGTAGATTGTAGATACTGTTTTATATCAAACAATGTTTATAATGTGTATATAGAATTGTTCACTGTAAAAAAAATGGCCAAAATGTGTTTTTTTTTTAATAAGTAACTTGACTATAAAATAAAGCCGTCCGTGGGACGACTGACCTCGTT NCBI Feature Key: mRNA NCBI Join Type: join"
     extracted re... 992..1224
                     /label="Extracted region from HHEX - 3087"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Extracted interval: 4593 -> 4825 Original Circular Sequence Length: 3366"
     attB2           complement(1214..1238)
                     /label="attB2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="annotation group: attB"
     protein_bind    complement(1225..1239)
                     /label="attR2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="bound_moiety: <html><body>LR Clonase™</body></html> gene: <html><body>mutant version of <i>att</i>R</body></html> note: <html><body>recombination site for the Gateway® LR reaction</body></html>"
     extracted re... 1225..1302
                     /label="Extracted region from CK490 - PB-cT3G-MG-cERP2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Extracted interval: 723 -> 800 Original Circular Sequence Length: 6786"
     CDS             1241..1267
                     /label="HA Tag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="created by: wyssuser"
     terminator      1268..1276
                     /label="STOPS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="organism: synthetic DNA construct mol_type: other DNA locus_tag: source:synthetic DNA construct(4) ApEinfo_label: source:synthetic DNA construct ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: source modified by: wyssuser"
     primer_bind     1280..1302
                     /label="oAMP123-PCR1_NGS_BC_Fwd"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     primer_bind     1280..1302
                     /label="BC_Fwd"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: ATACTCAGAAGATGTCACTCACC Hairpin Tm: None Tm: 57.4 Self Dimer Tm: None %GC: 43.5 created by: primer3 .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     extracted re... 1303..1372
                     /label="Extracted region from Barcode Fragment"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="Extracted interval: 8 -> 77 Original Circular Sequence Length: 6831"
     misc_difference 1307..1329
                     /label="Barcode linker 5'"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="created by: wyssuser"
     misc_feature    1330..1349
                     /label="20mer Barcode"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="created by: wyssuser modified by: wyssuser"
     misc_difference complement(1350..1372)
                     /label="Barcode linker 3'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 1373..1376
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Extracted interval: 223 -> 226 Original Circular Sequence Length: 6769"
     primer_bind     complement(1377..1399)
                     /label="BC_Rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3 .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     primer_bind     complement(1377..1399)
                     /label="oAMP124-PCR1_NGS_BC_Rev"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Sequence: CATTCATAGTTCTTGCTCAGTGG Hairpin Tm: None Tm: 57.5 Self Dimer Tm: None %GC: 43.5 created by: primer3 Extension: GAGTTCAGACGTGTGCTCTTCCGATCTNNN .hidden_Parent: h4/WfaYUZUO1YMa++1ncGxqKVrM.1614638334021.0"
     extracted re... 1377..1408
                     /label="Extracted region from Rev Primer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Extracted interval: -8 <- 23 Original Circular Sequence Length: 6769"
     motif           1400..1424
                     /label="FP NotI_BgIII cloning"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="created by: wyssuser modified by: wyssuser"
     extracted re... 1409..1442
                     /label="Extracted region from ATTR2-Right-FP modular"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Extracted interval: 259 -> 292 Original Circular Sequence Length: 6769"
     polyA_signal    1434..1568
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     extracted re... 1443..1598
                     /label="Extracted region from CK461-PB-cT3G-cERP2-Puro Base Vector #4-CMV_Enhancer - mCMV-Tet-Promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="Extracted interval: 636 -> 791 Original Circular Sequence Length: 6529"
     extracted re... 1599..4309
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="Extracted interval: 1035 -> 3745 Original Circular Sequence Length: 6769"
     repeat_region   complement(1840..1874)
                     /label="piggyBac left (5') inverted repeat"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac left (5') inverted repeat ApEinfo_label: piggyBac left (5') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region modified by: wyssuser"
     primer_bind     complement(1886..1901)
                     /label="M13F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Sequence: GTAAAACGACGGCCAG Hairpin Tm: None Tm: 52.4 Self Dimer Tm: 2.2 %GC: 56.3 created by: primer3"
     primer_bind     complement(2110..2129)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: pRS vectors, use to sequence yeast selectable marker locus_tag: pRS-marker ApEinfo_label: pRS-marker ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     2231..2253
                     /label="pGEX 3'"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: pGEX vectors, reverse primer locus_tag: pGEX 3' ApEinfo_label: pGEX 3' ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     complement(2292..2310)
                     /label="pBRforEco"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: pBR322 vectors, upsteam of EcoRI site, forward primer locus_tag: pBRforEco ApEinfo_label: pBRforEco ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     promoter        2378..2482
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: bla locus_tag: AmpR promoter ApEinfo_label: AmpR promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     CDS             2483..3343
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="codon_start: 1 gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, and related antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW locus_tag: AmpR ApEinfo_label: AmpR ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     primer_bind     complement(2701..2720)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: Ampicillin resistance gene, reverse primer locus_tag: Amp-R ApEinfo_label: Amp-R ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     rep_origin      3514..4102
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication locus_tag: ori ApEinfo_label: ori ApEinfo_fwdcolor: #999999 ApEinfo_revcolor: #999999 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: rep_origin"
     primer_bind     4003..4022
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: pBR322 origin, forward primer locus_tag: pBR322ori-F ApEinfo_label: pBR322ori-F ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     primer_bind     4256..4273
                     /label="L4440"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: L4440 vector, forward primer locus_tag: L4440 ApEinfo_label: L4440 ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind"
     extracted re... 4310..4398
                     /label="Extracted region from AP2-pBR322ori-F-SAPI.ab1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Extracted interval: 278 -> 366 Original Circular Sequence Length: 6769"
     extracted re... 4399..7267
                     /label="Extracted region from CK490 - PB-cT3G-MG-BsaIBC-FPMod-cERP2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 3835 -> 6703 Original Circular Sequence Length: 6769"
     protein_bind    4479..4500
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presenceof cAMP. locus_tag: CAP binding site ApEinfo_label: CAP binding site ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
     promoter        4515..4545
                     /label="lac promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: promoter for the E. coli lac operon locus_tag: lac promoter ApEinfo_label: lac promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     protein_bind    4553..4569
                     /label="lac operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). locus_tag: lac operator ApEinfo_label: lac operator ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: protein_bind"
     primer_bind     4577..4593
                     /label="M13R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: common sequencing primer, one of multiple similar variants locus_tag: M13 rev ApEinfo_label: M13 rev ApEinfo_fwdcolor: #14c0bd ApEinfo_revcolor: #4ec02b ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: primer_bind Sequence: CAGGAAACAGCTATGAC Hairpin Tm: None Tm: 49.1 Self Dimer Tm: None %GC: 47.1 created by: primer3 modified by: wyssuser"
     repeat_region   4619..4681
                     /label="piggyBac right (3') inverted repeat"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="note: piggyBac transposon-specific inverted terminal repeat sequence (ITR) locus_tag: piggyBac right (3') inverted repeat ApEinfo_label: piggyBac right (3') inverted repeat ApEinfo_fwdcolor: #0dfff7 ApEinfo_revcolor: #0dfff7 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: repeat_region"
     polyA_signal    4857..4938
                     /label="SV40 poly(A) signal(1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: SV40 polyadenylation signal locus_tag: SV40 poly(A) signal(1) ApEinfo_label: SV40 poly(A) signal ApEinfo_fwdcolor: #ff3eee ApEinfo_revcolor: #ff3eee ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: polyA_signal modified by: wyssuser"
     primer_bind     4965..4984
                     /label="Puro_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTTTCAGGTTCAGGGGGAGG Hairpin Tm: None Tm: 60.0 Self Dimer Tm: None %GC: 60.0 modified by: wyssuser"
     CDS             complement(5055..5654)
                     /label="Puromycin"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="created by: wyssuser"
     primer_bind     5540..5559
                     /label="Tet-ON_Fwd"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: GATCTACGGTATGGCGGGTC Hairpin Tm: None Tm: 59.8 Self Dimer Tm: None %GC: 60.0 Pair Dimer Tm: None Product Size: 35 annotation group: 69733439644894ee5c5e1-3592-4568-a0da-b1c47594b8cdFinal Plasmid modified by: wyssuser"
     CDS             complement(5655..5711)
                     /label="P2A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="codon_start: 1 product: 2A peptide from porcine teschovirus-1 polyprotein note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. translation: ATNFSLLKQAGDVEENPGP locus_tag: P2A ApEinfo_label: P2A ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     CDS             complement(5721..6464)
                     /label="Tet-On (3G)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="created by: wyssuser"
     primer_bind     complement(5862..5881)
                     /label="Puro_Rev_Seq Primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GTGGCCTGGAGAAACAGCTA Hairpin Tm: 37.2 Tm: 59.7 Self Dimer Tm: 3.3 %GC: 55.0 modified by: wyssuser"
     primer_bind     6107..6126
                     /label="CMV-Enhancer_Fwd_Seq Primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: GGAACGCGAGCTGATTTTCC Hairpin Tm: None Tm: 59.9 Self Dimer Tm: 15.9 %GC: 55.0 modified by: wyssuser"
     CDS             complement(6465..6488)
                     /label="FLAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="codon_start: 1 product: FLAG(R) epitope tag, followed by an enterokinasecleavage site translation: DYKDDDDK locus_tag: FLAG ApEinfo_label: FLAG ApEinfo_fwdcolor: #e9d024 ApEinfo_revcolor: #e9d024 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: CDS"
     regulatory      complement(6488..6497)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="regulatory_class: other note: vertebrate consensus sequence for strong initiationof translation (Kozak, 1987) locus_tag: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_label: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: regulatory modified by: wyssuser"
     promoter        complement(6517..6728)
                     /label="EF-1-alpha core promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: core promoter for human elongation factor EF-1-alpha locus_tag: EF-1-alpha core promoter ApEinfo_label: EF-1-alpha core promoter ApEinfo_fwdcolor: #346ee0 ApEinfo_revcolor: #346ee0 ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 NCBI Feature Key: promoter"
     regulatory      complement(6729..7108)
                     /label="CMV Enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="created by: wyssuser modified by: wyssuser"
     primer_bind     7257..7276
                     /label="pTet_Fwd"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="# Off-target Sites: 0 Off-target Template: Final Plasmid Sequence: TTTGAGTAGCGACTGACGGG annotation group: 646536956143517519a63-5614-47f8-a332-a32195d8b3a1Final Plasmid modified by: wyssuser Hairpin Tm: 42.8 Tm: 59.8 Self Dimer Tm: 3.5 %GC: 55.0 Pair Dimer Tm: None Product Size: 614"
     extracted re... 7268..7300
                     /label="Extracted region from Base Vector- PB-Tet-Puro"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="Extracted interval: 5833 -> 5865 Original Circular Sequence Length: 5865"
     extracted re... 7301..7333
                     /label="Extracted region from Puro Base Vector #2-CMV_Enhancer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Extracted interval: 6503 -> 6535 Original Circular Sequence Length: 6535"
     primer_bind     complement(7310..7328)
                     /label="CMV-Enhancer_Rev_Seq Primer"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="# Off-target Sites: 0 Off-target Template: Base Vector- PB-Tet-Puro Sequence: CACTCGAGTGGCTCCGGTG Hairpin Tm: 42.1 Tm: 62.6 Self Dimer Tm: 33.4 %GC: 68.4 modified by: wyssuser"
ORIGIN
        1 ATCACCTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC
       61 AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT
      121 TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA
      181 CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC
      241 AGTGATAGAG AACGTATAAG CTTGCCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA
      301 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGG GACCGATCCA
      361 GCCTACCACA AGTTTGTACA AAAAAGCAGG CTTAGCCACC ATGCAGTACC CGCACCCCGG
      421 GCCGGCGGCG GGCGCCGTGG GGGTGCCGCT GTACGCGCCC ACGCCGCTGC TGCAACCCGC
      481 ACACCCGACG CCCTTTTACA TCGAGGACAT CCTGGGCCGC GGGCCCGCCG CGCCCACGCC
      541 CGCCCCCACG CTGCCGTCCC CCAACTCCTC CTTCACCAGC CTCGTGTCCC CCTACCGGAC
      601 CCCGGTGTAC GAGCCCACGC CGATCCATCC AGCCTTCTCG CACCACTCCG CCGCCGCGCT
      661 GGCCGCTGCC TACGGACCCG GCGGCTTCGG GGGCCCTCTG TACCCCTTCC CGCGGACGGT
      721 GAACGACTAC ACGCACGCCC TGCTCCGCCA CGACCCCCTG GGCAAACCTC TACTCTGGAG
      781 CCCCTTCTTG CAGAGGCCTC TGCATAAAAG GAAAGGCGGC CAGGTGAGAT TCTCCAACGA
      841 CCAGACCATC GAGCTGGAGA AGAAATTCGA GACGCAGAAA TATCTCTCTC CGCCCGAGAG
      901 GAAGCGTCTG GCCAAGATGC TGCAGCTCAG CGAGAGACAG GTCAAAACCT GGTTTCAGAA
      961 TCGACGCGCT AAATGGAGGA GACTAAAACA GGAGAACCCT CAAAGCAATA AAAAAGAAGA
     1021 ACTGGAAAGT TTGGACAGTT CCTGTGATCA GAGGCAAGAT TTGCCCAGTG AACAGAATAA
     1081 AGGTGCTTCT TTGGATAGCT CTCAATGTTC GCCCTCCCCT GCCTCCCAGG AAGACCTTGA
     1141 ATCAGAGATT TCAGAGGATT CTGATCAGGA AGTGGACATT GAGGGCGATA AAAGCTATTT
     1201 TAATGCTGGA TGATACCCAG CTTTCTTGTA CAAAGTGGTA TACCCATACG ATGTTCCAGA
     1261 TTACGCTTAG TAATGAGAAA TACTCAGAAG ATGTCACTCA CCATAGTCCG CAGTGAAAGA
     1321 TAAATGATCN NNNNNNNNNN NNNNNNNNNG TTTTAGAGCT AGAAATAGCA AGTAGGCCAC
     1381 TGAGCAAGAA CTATGAATGA GATCTTTAGA CATATTGCGG CCGCTTTCTC CGCGATCCAG
     1441 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT
     1501 GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA
     1561 AACAAGTTAT CTATAACAAG AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG
     1621 AAATAACAAT ATAATTATCG TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG
     1681 CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA
     1741 AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT
     1801 TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA
     1861 CTATCTTTCT AGGGTTAAGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     1921 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     1981 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     2041 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG
     2101 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC
     2161 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT
     2221 GACCATGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACACGCG
     2281 AGTTCGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
     2341 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
     2401 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
     2461 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT
     2521 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA
     2581 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA
     2641 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT
     2701 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT
     2761 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA
     2821 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC
     2881 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT
     2941 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA
     3001 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC
     3061 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA
     3121 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC
     3181 TGGAGCCGGT GAGCGTGGTT CACGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC
     3241 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG
     3301 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA
     3361 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA
     3421 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC
     3481 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     3541 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     3601 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     3661 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     3721 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     3781 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     3841 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     3901 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     3961 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     4021 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     4081 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
     4141 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
     4201 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
     4261 GTCAGTGAGC GAGGAAGCGG ATGAGCGCCC AATACGCAAA CCGCCTCTCA CCGCGCGTTG
     4321 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
     4381 CAACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
     4441 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
     4501 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
     4561 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGGT CGACTTAACC
     4621 CTAGAAAGAT AATCATATTG TGACGTACGT TAAAGATAAT CATGCGTAAA ATTGACGCAT
     4681 GTGTTTTATC GGTCTGTATA TCGAGGTTTA TTTATTAATT TGAATAGATA TTAAGTTTTA
     4741 TTATATTTAC ACTTACATAC TAATAATAAA TTCAACAAAC AATTTATTTA TGTTTATTTA
     4801 TTTATTAAAA AAAAACAAAA ACTCAAAATT TCTTCTATAA AGTAACAAAA CTTTTAGCAG
     4861 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
     4921 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
     4981 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
     5041 GATCCTCTGG AGATTCATGC GCCAGGTTTT CTGGTCATGC ACCACGTTCG TGGGCCCTCA
     5101 GGAACCTCCA CGTCAGCGGT AACTGTGAAG CCCAGTCGCT CGTAAAAAGG AAGATTCCGG
     5161 GGAGCACTGG TTTCGAGGAA CGCCGGCACG CCAGCCCGTT CCGCGGCCTC AACACCAGGA
     5221 AGAACGACAG CGCTTCCCAA ACCTTTACCT TGGTGATCTG GGCTGACACC GACAGTGGCA
     5281 AGAAACCACG CAGGCTCCTT TGGTCTATGA GGAGCCAGCA AGCCCTCCAT TTGCTGTTGT
     5341 GCCGCCAACC TACTCCCTGA CAGCTCGGCC ATCCGCGGAC CTATTTCCGC GAACACTGCG
     5401 CCGGCTTCGA CGCTTTCCGG AGTAGTCCAG ACCGCAACTG CGGCACCATC GTCCGCAACC
     5461 CAAACTTTAC CAATGTCCAG ACCCACGCGC GTAAGAAACA GCTCTTGCAA CTCAGTTACC
     5521 CTCTCTATGT GTCTGTCCGG ATCTACGGTA TGGCGGGTCG CTGGATAATC TGCAAATGCT
     5581 GCGGCAAGTG TTCGGACAGC CCGTGGTACA TCGTCACGGG TAGCGAGCCT AACAGTGGGC
     5641 TTATATTCCG TCATGGGGCC GGGGTTCTCC TCCACGTCGC CGGCCTGCTT CAGCAGGCTG
     5701 AAGTTGGTGG CGCCGCTGCC CCCGGGGAGC ATGTCAAGGT CAAAATCGTC AAGAGCGTCA
     5761 GCAGGCAGCA TATCAAGGTC AAAGTCGTCA AGGGCATCGG CTGGGAGCAT GTCTAAGTCA
     5821 AAATCGTCAA GGGCGTCGGT CGGCCCGCCG CTTTCGCACT TTAGCTGTTT CTCCAGGCCA
     5881 CATATGATTA GTTCCAGGCC GAAAAGGAAG GCAGGTTCGG CTCCCTGCCG GTCGAACAGC
     5941 TCAATTGCTT GTTTCAGAAG TGGGGGCATA GAATCGGTGG TAGGTGTCTC TCTTTCCTCT
     6001 TTTGCTACTT GATGCTCCTG TTCCTCCAAT ACGCAGCCCA GTGTAAAGTG GCCCACGGCG
     6061 GACAGAGCGT ACAGTGCGTT CTCCAGGGAG AAGCCTTGCT GACACAGGAA CGCGAGCTGA
     6121 TTTTCCAGGG TTTCGTACTG TTTCTCTGTT GGGCGGGTGC CGAGATGCAC TTTAGCCCCG
     6181 TCACGATGTG AGAGGAGAGC ACAGCGGTAT GACTTGGCGT TGTTCCGCAG AAAGTCTTGC
     6241 CATGACTCGC CTTCCAGGGG GCAGGAGTGG GTATGATGCC TGTCCAGCAT CTCGATTGGC
     6301 AGGGCATCGA GCAGGGCCCG CTTGTTCTTC ACGTGCCAGT ACAGGGTAGG CTGCTCAACT
     6361 CCCAGCTTTT GAGCGAGTTT CCTTGTCGTC AGGCCTTCGA TACCGACTCC ATTGAGTAAT
     6421 TCCAGAGCAG AGTTTATGAC TTTGCTCTTG TCCAGTCTAG ACATCTTATC GTCATCGTCT
     6481 TTGTAATCCA TGGTGGCGGA TCCCGCGTCA CGACACCTGT GTTCTGGCGG CAAACCCGTT
     6541 GCGAAAAAGA ACGTTCACGG CGACTACTGC ACTTATATAC GGTTCTCCCC CACCCTCGGG
     6601 AAAAAGGCGG AGCCAGTACA CGACATCACT TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA
     6661 GGCACCGGTT CAATTGCCGA CCCCTCCCCC CAACTTCTCG GGCACTGTGG GCCATGTGCG
     6721 CTCTGCCCCA TGGTAATAGC CATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTGC
     6781 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA
     6841 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA
     6901 TACTCCACCC ATTGACGTCA ATGGAAAGTG CCTATTGGCG TTACTATGGG AACATACGTC
     6961 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA
     7021 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC
     7081 TATTAATAAC TAGTCAATAA TCAATGTCAT AAGCTCTAAT CGCATAATCG AGCTAAGAGC
     7141 GATTAATATG CTTAGCTAGC TTAGCTAAGC TAGATTTCTC TATTCTCCCA CAATATATAG
     7201 CAGCTATTAG CTTAGCAGTC CGAATACGAT CGTCCACGAT CCATAAGCGT AACACATTTG
     7261 AGTAGCGACT GACGGGCACC GGAGCCACTC GAGTGGAATT ACTGACGGGC ACCGGAGCCA
     7321 CTCGAGTGGA ATT
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